Processing 1 helix: Transmembrane helix 1 from 316 (in) to 346 (out) : 4645.68 Score = 4.645684 Processing 1 helix: Transmembrane helix 1 from 316 (out) to 346 (in) : 4593.82 Score = 4.593815 Processing 23 helices: Transmembrane helix 1 from 8 (in) to 23 (out) : 310.161 Transmembrane helix 2 from 27 (out) to 42 (in) : 1321.46 Transmembrane helix 3 from 46 (in) to 61 (out) : 136.822 Transmembrane helix 4 from 65 (out) to 80 (in) : 543.209 Transmembrane helix 5 from 84 (in) to 99 (out) : 931.505 Transmembrane helix 6 from 103 (out) to 118 (in) : 438.257 Transmembrane helix 7 from 122 (in) to 137 (out) : 129.37 Transmembrane helix 8 from 141 (out) to 156 (in) : 1269.93 Transmembrane helix 9 from 160 (in) to 175 (out) : 421.797 Transmembrane helix 10 from 179 (out) to 194 (in) : 1159.43 Transmembrane helix 11 from 198 (in) to 213 (out) : -796.222 Transmembrane helix 12 from 217 (out) to 232 (in) : -1764.51 Transmembrane helix 13 from 236 (in) to 251 (out) : -79.545 Transmembrane helix 14 from 255 (out) to 270 (in) : 1843.11 Transmembrane helix 15 from 274 (in) to 289 (out) : 633.184 Transmembrane helix 16 from 293 (out) to 308 (in) : 1478.19 Transmembrane helix 17 from 312 (in) to 327 (out) : 2716.3 Transmembrane helix 18 from 331 (out) to 346 (in) : 2089.43 Transmembrane helix 19 from 350 (in) to 365 (out) : 452.678 Transmembrane helix 20 from 369 (out) to 384 (in) : 648.952 Transmembrane helix 21 from 388 (in) to 403 (out) : 688.423 Transmembrane helix 22 from 407 (out) to 422 (in) : 1994.3 Transmembrane helix 23 from 426 (in) to 441 (out) : -878.209 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 8 (out) to 23 (in) : 293.039 Transmembrane helix 2 from 27 (in) to 42 (out) : 1347.14 Transmembrane helix 3 from 46 (out) to 61 (in) : 148.578 Transmembrane helix 4 from 65 (in) to 80 (out) : 520.691 Transmembrane helix 5 from 84 (out) to 99 (in) : 899.895 Transmembrane helix 6 from 103 (in) to 118 (out) : 430.143 Transmembrane helix 7 from 122 (out) to 137 (in) : 139.33 Transmembrane helix 8 from 141 (in) to 156 (out) : 1287.67 Transmembrane helix 9 from 160 (out) to 175 (in) : 423.203 Transmembrane helix 10 from 179 (in) to 194 (out) : 1150.57 Transmembrane helix 11 from 198 (out) to 213 (in) : -810.378 Transmembrane helix 12 from 231 (in) to 246 (out) : -1088.03 Transmembrane helix 13 from 250 (out) to 265 (in) : 2325.65 Transmembrane helix 14 from 269 (in) to 284 (out) : 265.624 Transmembrane helix 15 from 288 (out) to 303 (in) : 2312.02 Transmembrane helix 16 from 307 (in) to 322 (out) : 1883.19 Transmembrane helix 17 from 326 (out) to 341 (in) : 1883.41 Transmembrane helix 18 from 345 (in) to 360 (out) : 1215.91 Transmembrane helix 19 from 364 (out) to 379 (in) : 286.212 Transmembrane helix 20 from 383 (in) to 398 (out) : 668.833 Transmembrane helix 21 from 402 (out) to 417 (in) : 2596.59 Transmembrane helix 22 from 421 (in) to 436 (out) : -614.201 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 8 (in) to 23 (out) : 310.161 Transmembrane helix 2 from 27 (out) to 42 (in) : 1321.46 Transmembrane helix 3 from 46 (in) to 61 (out) : 136.822 Transmembrane helix 4 from 65 (out) to 80 (in) : 543.209 Transmembrane helix 5 from 84 (in) to 99 (out) : 931.505 Transmembrane helix 6 from 103 (out) to 118 (in) : 438.257 Transmembrane helix 7 from 122 (in) to 137 (out) : 129.37 Transmembrane helix 8 from 141 (out) to 156 (in) : 1269.93 Transmembrane helix 9 from 160 (in) to 175 (out) : 421.797 Transmembrane helix 10 from 179 (out) to 194 (in) : 1159.43 Transmembrane helix 11 from 198 (in) to 213 (out) : -796.222 Transmembrane helix 12 from 246 (out) to 261 (in) : 2067.53 Transmembrane helix 13 from 265 (in) to 280 (out) : 507.032 Transmembrane helix 14 from 284 (out) to 303 (in) : 2582.64 Transmembrane helix 15 from 307 (in) to 322 (out) : 1883.19 Transmembrane helix 16 from 326 (out) to 341 (in) : 1883.41 Transmembrane helix 17 from 345 (in) to 360 (out) : 1215.91 Transmembrane helix 18 from 364 (out) to 379 (in) : 286.212 Transmembrane helix 19 from 383 (in) to 398 (out) : 668.833 Transmembrane helix 20 from 402 (out) to 417 (in) : 2596.59 Transmembrane helix 21 from 421 (in) to 436 (out) : -614.201 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 14 (out) to 29 (in) : 1263.59 Transmembrane helix 2 from 33 (in) to 48 (out) : 581.844 Transmembrane helix 3 from 52 (out) to 67 (in) : 727.098 Transmembrane helix 4 from 71 (in) to 86 (out) : 165.814 Transmembrane helix 5 from 90 (out) to 105 (in) : 834.405 Transmembrane helix 6 from 109 (in) to 124 (out) : 657.441 Transmembrane helix 7 from 128 (out) to 143 (in) : -135.042 Transmembrane helix 8 from 147 (in) to 166 (out) : 1950.62 Transmembrane helix 9 from 170 (out) to 185 (in) : 887.754 Transmembrane helix 10 from 189 (in) to 204 (out) : -21.926 Transmembrane helix 11 from 246 (out) to 261 (in) : 2067.53 Transmembrane helix 12 from 265 (in) to 280 (out) : 507.032 Transmembrane helix 13 from 284 (out) to 303 (in) : 2582.64 Transmembrane helix 14 from 307 (in) to 322 (out) : 1883.19 Transmembrane helix 15 from 326 (out) to 341 (in) : 1883.41 Transmembrane helix 16 from 345 (in) to 360 (out) : 1215.91 Transmembrane helix 17 from 364 (out) to 379 (in) : 286.212 Transmembrane helix 18 from 383 (in) to 398 (out) : 668.833 Transmembrane helix 19 from 402 (out) to 417 (in) : 2596.59 Transmembrane helix 20 from 421 (in) to 436 (out) : -614.201 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : 1278.11 Transmembrane helix 2 from 33 (out) to 48 (in) : 584.256 Transmembrane helix 3 from 52 (in) to 67 (out) : 722.302 Transmembrane helix 4 from 71 (out) to 86 (in) : 179.486 Transmembrane helix 5 from 90 (in) to 105 (out) : 847.895 Transmembrane helix 6 from 109 (out) to 124 (in) : 679.759 Transmembrane helix 7 from 128 (in) to 143 (out) : -132.858 Transmembrane helix 8 from 147 (out) to 162 (in) : 1784.06 Transmembrane helix 9 from 166 (in) to 181 (out) : 549.899 Transmembrane helix 10 from 185 (out) to 200 (in) : 459.338 Transmembrane helix 11 from 246 (in) to 261 (out) : 2118.07 Transmembrane helix 12 from 265 (out) to 280 (in) : 503.168 Transmembrane helix 13 from 284 (in) to 304 (out) : 2540.05 Transmembrane helix 14 from 311 (out) to 330 (in) : 2957.12 Transmembrane helix 15 from 334 (in) to 349 (out) : 2379.21 Transmembrane helix 16 from 353 (out) to 368 (in) : 244.649 Transmembrane helix 17 from 372 (in) to 387 (out) : 842.125 Transmembrane helix 18 from 391 (out) to 406 (in) : 1088.67 Transmembrane helix 19 from 410 (in) to 425 (out) : 1347.65 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 14 (out) to 29 (in) : 1263.59 Transmembrane helix 2 from 33 (in) to 48 (out) : 581.844 Transmembrane helix 3 from 52 (out) to 67 (in) : 727.098 Transmembrane helix 4 from 71 (in) to 86 (out) : 165.814 Transmembrane helix 5 from 90 (out) to 105 (in) : 834.405 Transmembrane helix 6 from 109 (in) to 124 (out) : 657.441 Transmembrane helix 7 from 128 (out) to 143 (in) : -135.042 Transmembrane helix 8 from 147 (in) to 166 (out) : 1950.62 Transmembrane helix 9 from 170 (out) to 185 (in) : 887.754 Transmembrane helix 10 from 189 (in) to 204 (out) : -21.926 Transmembrane helix 11 from 246 (out) to 261 (in) : 2067.53 Transmembrane helix 12 from 265 (in) to 280 (out) : 507.032 Transmembrane helix 13 from 284 (out) to 304 (in) : 2588.55 Transmembrane helix 14 from 310 (in) to 330 (out) : 3011.55 Transmembrane helix 15 from 335 (out) to 356 (in) : 2792.33 Transmembrane helix 16 from 360 (in) to 375 (out) : 106.725 Transmembrane helix 17 from 379 (out) to 394 (in) : 932 Transmembrane helix 18 from 401 (in) to 420 (out) : 2712.2 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : 1278.11 Transmembrane helix 2 from 33 (out) to 48 (in) : 584.256 Transmembrane helix 3 from 52 (in) to 67 (out) : 722.302 Transmembrane helix 4 from 71 (out) to 86 (in) : 179.486 Transmembrane helix 5 from 90 (in) to 105 (out) : 847.895 Transmembrane helix 6 from 109 (out) to 124 (in) : 679.759 Transmembrane helix 7 from 128 (in) to 143 (out) : -132.858 Transmembrane helix 8 from 147 (out) to 162 (in) : 1784.06 Transmembrane helix 9 from 166 (in) to 181 (out) : 549.899 Transmembrane helix 10 from 185 (out) to 200 (in) : 459.338 Transmembrane helix 11 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 12 from 283 (out) to 304 (in) : 2608.84 Transmembrane helix 13 from 310 (in) to 330 (out) : 3011.55 Transmembrane helix 14 from 335 (out) to 356 (in) : 2792.33 Transmembrane helix 15 from 360 (in) to 375 (out) : 106.725 Transmembrane helix 16 from 379 (out) to 394 (in) : 932 Transmembrane helix 17 from 401 (in) to 420 (out) : 2712.2 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 17 (out) to 33 (in) : 1760.74 Transmembrane helix 2 from 37 (in) to 52 (out) : 126.152 Transmembrane helix 3 from 57 (out) to 74 (in) : 1109.2 Transmembrane helix 4 from 84 (in) to 103 (out) : 1099.86 Transmembrane helix 5 from 108 (out) to 123 (in) : 627.993 Transmembrane helix 6 from 127 (in) to 142 (out) : -115.015 Transmembrane helix 7 from 146 (out) to 162 (in) : 1829.89 Transmembrane helix 8 from 166 (in) to 181 (out) : 549.899 Transmembrane helix 9 from 185 (out) to 200 (in) : 459.338 Transmembrane helix 10 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 11 from 283 (out) to 304 (in) : 2608.84 Transmembrane helix 12 from 310 (in) to 330 (out) : 3011.55 Transmembrane helix 13 from 335 (out) to 356 (in) : 2792.33 Transmembrane helix 14 from 360 (in) to 375 (out) : 106.725 Transmembrane helix 15 from 379 (out) to 394 (in) : 932 Transmembrane helix 16 from 401 (in) to 420 (out) : 2712.2 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 17 (in) to 33 (out) : 1772.86 Transmembrane helix 2 from 37 (out) to 52 (in) : 132.348 Transmembrane helix 3 from 56 (in) to 74 (out) : 1092.98 Transmembrane helix 4 from 84 (out) to 103 (in) : 1062.94 Transmembrane helix 5 from 107 (in) to 122 (out) : 558.526 Transmembrane helix 6 from 126 (out) to 141 (in) : -87.836 Transmembrane helix 7 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 8 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 9 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 10 from 283 (out) to 304 (in) : 2608.84 Transmembrane helix 11 from 310 (in) to 330 (out) : 3011.55 Transmembrane helix 12 from 335 (out) to 356 (in) : 2792.33 Transmembrane helix 13 from 360 (in) to 375 (out) : 106.725 Transmembrane helix 14 from 379 (out) to 394 (in) : 932 Transmembrane helix 15 from 401 (in) to 420 (out) : 2712.2 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 17 (out) to 33 (in) : 1760.74 Transmembrane helix 2 from 37 (in) to 52 (out) : 126.152 Transmembrane helix 3 from 57 (out) to 74 (in) : 1109.2 Transmembrane helix 4 from 84 (in) to 103 (out) : 1099.86 Transmembrane helix 5 from 109 (out) to 129 (in) : 779.736 Transmembrane helix 6 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 7 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 8 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 9 from 283 (out) to 304 (in) : 2608.84 Transmembrane helix 10 from 310 (in) to 330 (out) : 3011.55 Transmembrane helix 11 from 335 (out) to 356 (in) : 2792.33 Transmembrane helix 12 from 360 (in) to 375 (out) : 106.725 Transmembrane helix 13 from 379 (out) to 394 (in) : 932 Transmembrane helix 14 from 401 (in) to 420 (out) : 2712.2 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 17 (in) to 42 (out) : 2240.41 Transmembrane helix 2 from 57 (out) to 74 (in) : 1109.2 Transmembrane helix 3 from 84 (in) to 103 (out) : 1099.86 Transmembrane helix 4 from 109 (out) to 129 (in) : 779.736 Transmembrane helix 5 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 6 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 7 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 8 from 283 (out) to 304 (in) : 2608.84 Transmembrane helix 9 from 310 (in) to 330 (out) : 3011.55 Transmembrane helix 10 from 335 (out) to 356 (in) : 2792.33 Transmembrane helix 11 from 360 (in) to 375 (out) : 106.725 Transmembrane helix 12 from 379 (out) to 394 (in) : 932 Transmembrane helix 13 from 401 (in) to 420 (out) : 2712.2 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 17 (out) to 42 (in) : 2200.39 Transmembrane helix 2 from 57 (in) to 87 (out) : 1126.56 Transmembrane helix 3 from 91 (out) to 121 (in) : 1315.65 Transmembrane helix 4 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 5 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 6 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 7 from 283 (out) to 304 (in) : 2608.84 Transmembrane helix 8 from 310 (in) to 330 (out) : 3011.55 Transmembrane helix 9 from 335 (out) to 356 (in) : 2792.33 Transmembrane helix 10 from 360 (in) to 375 (out) : 106.725 Transmembrane helix 11 from 379 (out) to 394 (in) : 932 Transmembrane helix 12 from 401 (in) to 420 (out) : 2712.2 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 17 (in) to 42 (out) : 2240.41 Transmembrane helix 2 from 57 (out) to 74 (in) : 1109.2 Transmembrane helix 3 from 84 (in) to 103 (out) : 1099.86 Transmembrane helix 4 from 109 (out) to 129 (in) : 779.736 Transmembrane helix 5 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 6 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 7 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 8 from 283 (out) to 304 (in) : 2608.84 Transmembrane helix 9 from 310 (in) to 330 (out) : 3011.55 Transmembrane helix 10 from 335 (out) to 361 (in) : 2830.03 Transmembrane helix 11 from 387 (in) to 417 (out) : 2993.67 Score = 23.199192 Processing 10 helices: Transmembrane helix 1 from 17 (out) to 42 (in) : 2200.39 Transmembrane helix 2 from 57 (in) to 87 (out) : 1126.56 Transmembrane helix 3 from 91 (out) to 121 (in) : 1315.65 Transmembrane helix 4 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 5 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 6 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 7 from 283 (out) to 304 (in) : 2608.84 Transmembrane helix 8 from 310 (in) to 330 (out) : 3011.55 Transmembrane helix 9 from 335 (out) to 361 (in) : 2830.03 Transmembrane helix 10 from 387 (in) to 417 (out) : 2993.67 Score = 22.507620 Processing 9 helices: Transmembrane helix 1 from 17 (in) to 42 (out) : 2240.41 Transmembrane helix 2 from 88 (out) to 118 (in) : 1353.57 Transmembrane helix 3 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 4 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 5 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 6 from 283 (out) to 304 (in) : 2608.84 Transmembrane helix 7 from 310 (in) to 330 (out) : 3011.55 Transmembrane helix 8 from 335 (out) to 361 (in) : 2830.03 Transmembrane helix 9 from 387 (in) to 417 (out) : 2993.67 Score = 21.526861 Processing 8 helices: Transmembrane helix 1 from 17 (out) to 42 (in) : 2200.39 Transmembrane helix 2 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 3 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 4 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 5 from 283 (out) to 304 (in) : 2608.84 Transmembrane helix 6 from 310 (in) to 330 (out) : 3011.55 Transmembrane helix 7 from 335 (out) to 361 (in) : 2830.03 Transmembrane helix 8 from 387 (in) to 417 (out) : 2993.67 Score = 20.062429 Processing 7 helices: Transmembrane helix 1 from 17 (in) to 42 (out) : 2240.41 Transmembrane helix 2 from 88 (out) to 118 (in) : 1353.57 Transmembrane helix 3 from 150 (in) to 180 (out) : 2115.47 Transmembrane helix 4 from 247 (out) to 274 (in) : 2877.37 Transmembrane helix 5 from 285 (in) to 315 (out) : 2970.79 Transmembrane helix 6 from 319 (out) to 349 (in) : 4356.41 Transmembrane helix 7 from 387 (in) to 417 (out) : 2993.67 Score = 18.854624 Processing 6 helices: Transmembrane helix 1 from 17 (out) to 42 (in) : 2200.39 Transmembrane helix 2 from 150 (in) to 180 (out) : 2115.47 Transmembrane helix 3 from 247 (out) to 274 (in) : 2877.37 Transmembrane helix 4 from 285 (in) to 315 (out) : 2970.79 Transmembrane helix 5 from 319 (out) to 349 (in) : 4356.41 Transmembrane helix 6 from 387 (in) to 417 (out) : 2993.67 Score = 17.390007 Processing 5 helices: Transmembrane helix 1 from 17 (in) to 42 (out) : 2240.41 Transmembrane helix 2 from 247 (out) to 274 (in) : 2877.37 Transmembrane helix 3 from 285 (in) to 315 (out) : 2970.79 Transmembrane helix 4 from 319 (out) to 349 (in) : 4356.41 Transmembrane helix 5 from 387 (in) to 417 (out) : 2993.67 Score = 15.360871 Processing 4 helices: Transmembrane helix 1 from 247 (out) to 274 (in) : 2877.37 Transmembrane helix 2 from 285 (in) to 315 (out) : 2970.79 Transmembrane helix 3 from 319 (out) to 349 (in) : 4356.41 Transmembrane helix 4 from 387 (in) to 417 (out) : 2993.67 Score = 13.097303 Processing 3 helices: Transmembrane helix 1 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 2 from 316 (out) to 346 (in) : 4593.82 Transmembrane helix 3 from 387 (in) to 417 (out) : 2993.67 Score = 10.502863 Processing 2 helices: Transmembrane helix 1 from 316 (out) to 346 (in) : 4593.82 Transmembrane helix 2 from 387 (in) to 417 (out) : 2993.67 Score = 7.507397 Processing 23 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -398.422 Transmembrane helix 2 from 23 (in) to 38 (out) : 1657.44 Transmembrane helix 3 from 42 (out) to 57 (in) : -63.234 Transmembrane helix 4 from 61 (in) to 76 (out) : 876.995 Transmembrane helix 5 from 80 (out) to 95 (in) : 592.034 Transmembrane helix 6 from 99 (in) to 114 (out) : 461.772 Transmembrane helix 7 from 118 (out) to 133 (in) : 383.118 Transmembrane helix 8 from 137 (in) to 152 (out) : 614.899 Transmembrane helix 9 from 156 (out) to 171 (in) : 879.777 Transmembrane helix 10 from 175 (in) to 190 (out) : 1228.14 Transmembrane helix 11 from 194 (out) to 209 (in) : -527.762 Transmembrane helix 12 from 213 (in) to 228 (out) : -1723.54 Transmembrane helix 13 from 232 (out) to 247 (in) : -949.263 Transmembrane helix 14 from 251 (in) to 266 (out) : 2332.18 Transmembrane helix 15 from 270 (out) to 285 (in) : 261.817 Transmembrane helix 16 from 289 (in) to 304 (out) : 2167.6 Transmembrane helix 17 from 308 (out) to 323 (in) : 2020.78 Transmembrane helix 18 from 327 (in) to 342 (out) : 1944.7 Transmembrane helix 19 from 346 (out) to 361 (in) : 1073.04 Transmembrane helix 20 from 365 (in) to 380 (out) : 365.478 Transmembrane helix 21 from 384 (out) to 399 (in) : 584.907 Transmembrane helix 22 from 403 (in) to 418 (out) : 2553.73 Transmembrane helix 23 from 422 (out) to 437 (in) : -697.414 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 8 (in) to 23 (out) : 310.161 Transmembrane helix 2 from 27 (out) to 42 (in) : 1321.46 Transmembrane helix 3 from 46 (in) to 61 (out) : 136.822 Transmembrane helix 4 from 65 (out) to 80 (in) : 543.209 Transmembrane helix 5 from 84 (in) to 99 (out) : 931.505 Transmembrane helix 6 from 103 (out) to 118 (in) : 438.257 Transmembrane helix 7 from 122 (in) to 137 (out) : 129.37 Transmembrane helix 8 from 141 (out) to 156 (in) : 1269.93 Transmembrane helix 9 from 160 (in) to 175 (out) : 421.797 Transmembrane helix 10 from 179 (out) to 194 (in) : 1159.43 Transmembrane helix 11 from 198 (in) to 213 (out) : -796.222 Transmembrane helix 12 from 230 (out) to 245 (in) : -1215.16 Transmembrane helix 13 from 249 (in) to 264 (out) : 2382.87 Transmembrane helix 14 from 268 (out) to 283 (in) : 272.227 Transmembrane helix 15 from 287 (in) to 303 (out) : 2386.92 Transmembrane helix 16 from 307 (out) to 322 (in) : 1835.2 Transmembrane helix 17 from 326 (in) to 341 (out) : 1900.09 Transmembrane helix 18 from 345 (out) to 360 (in) : 1245.59 Transmembrane helix 19 from 364 (in) to 379 (out) : 295.188 Transmembrane helix 20 from 383 (out) to 398 (in) : 650.667 Transmembrane helix 21 from 402 (in) to 417 (out) : 2568.81 Transmembrane helix 22 from 421 (out) to 436 (in) : -613.799 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 8 (out) to 23 (in) : 293.039 Transmembrane helix 2 from 27 (in) to 42 (out) : 1347.14 Transmembrane helix 3 from 46 (out) to 61 (in) : 148.578 Transmembrane helix 4 from 65 (in) to 80 (out) : 520.691 Transmembrane helix 5 from 84 (out) to 99 (in) : 899.895 Transmembrane helix 6 from 103 (in) to 118 (out) : 430.143 Transmembrane helix 7 from 122 (out) to 137 (in) : 139.33 Transmembrane helix 8 from 141 (in) to 156 (out) : 1287.67 Transmembrane helix 9 from 160 (out) to 175 (in) : 423.203 Transmembrane helix 10 from 179 (in) to 194 (out) : 1150.57 Transmembrane helix 11 from 198 (out) to 213 (in) : -810.378 Transmembrane helix 12 from 246 (in) to 261 (out) : 2118.07 Transmembrane helix 13 from 265 (out) to 280 (in) : 503.168 Transmembrane helix 14 from 284 (in) to 303 (out) : 2538.56 Transmembrane helix 15 from 307 (out) to 322 (in) : 1835.2 Transmembrane helix 16 from 326 (in) to 341 (out) : 1900.09 Transmembrane helix 17 from 345 (out) to 360 (in) : 1245.59 Transmembrane helix 18 from 364 (in) to 379 (out) : 295.188 Transmembrane helix 19 from 383 (out) to 398 (in) : 650.667 Transmembrane helix 20 from 402 (in) to 417 (out) : 2568.81 Transmembrane helix 21 from 421 (out) to 436 (in) : -613.799 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : 1278.11 Transmembrane helix 2 from 33 (out) to 48 (in) : 584.256 Transmembrane helix 3 from 52 (in) to 67 (out) : 722.302 Transmembrane helix 4 from 71 (out) to 86 (in) : 179.486 Transmembrane helix 5 from 90 (in) to 105 (out) : 847.895 Transmembrane helix 6 from 109 (out) to 124 (in) : 679.759 Transmembrane helix 7 from 128 (in) to 143 (out) : -132.858 Transmembrane helix 8 from 147 (out) to 162 (in) : 1784.06 Transmembrane helix 9 from 166 (in) to 181 (out) : 549.899 Transmembrane helix 10 from 185 (out) to 200 (in) : 459.338 Transmembrane helix 11 from 246 (in) to 261 (out) : 2118.07 Transmembrane helix 12 from 265 (out) to 280 (in) : 503.168 Transmembrane helix 13 from 284 (in) to 303 (out) : 2538.56 Transmembrane helix 14 from 307 (out) to 322 (in) : 1835.2 Transmembrane helix 15 from 326 (in) to 341 (out) : 1900.09 Transmembrane helix 16 from 345 (out) to 360 (in) : 1245.59 Transmembrane helix 17 from 364 (in) to 379 (out) : 295.188 Transmembrane helix 18 from 383 (out) to 398 (in) : 650.667 Transmembrane helix 19 from 402 (in) to 417 (out) : 2568.81 Transmembrane helix 20 from 421 (out) to 436 (in) : -613.799 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 14 (out) to 29 (in) : 1263.59 Transmembrane helix 2 from 33 (in) to 48 (out) : 581.844 Transmembrane helix 3 from 52 (out) to 67 (in) : 727.098 Transmembrane helix 4 from 71 (in) to 86 (out) : 165.814 Transmembrane helix 5 from 90 (out) to 105 (in) : 834.405 Transmembrane helix 6 from 109 (in) to 124 (out) : 657.441 Transmembrane helix 7 from 128 (out) to 143 (in) : -135.042 Transmembrane helix 8 from 147 (in) to 166 (out) : 1950.62 Transmembrane helix 9 from 170 (out) to 185 (in) : 887.754 Transmembrane helix 10 from 189 (in) to 204 (out) : -21.926 Transmembrane helix 11 from 246 (out) to 261 (in) : 2067.53 Transmembrane helix 12 from 265 (in) to 280 (out) : 507.032 Transmembrane helix 13 from 284 (out) to 304 (in) : 2588.55 Transmembrane helix 14 from 310 (in) to 330 (out) : 3011.55 Transmembrane helix 15 from 334 (out) to 349 (in) : 2375.39 Transmembrane helix 16 from 353 (in) to 368 (out) : 221.151 Transmembrane helix 17 from 372 (out) to 387 (in) : 838.875 Transmembrane helix 18 from 391 (in) to 406 (out) : 1087.33 Transmembrane helix 19 from 410 (out) to 425 (in) : 1365.15 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : 1278.11 Transmembrane helix 2 from 33 (out) to 48 (in) : 584.256 Transmembrane helix 3 from 52 (in) to 67 (out) : 722.302 Transmembrane helix 4 from 71 (out) to 86 (in) : 179.486 Transmembrane helix 5 from 90 (in) to 105 (out) : 847.895 Transmembrane helix 6 from 109 (out) to 124 (in) : 679.759 Transmembrane helix 7 from 128 (in) to 143 (out) : -132.858 Transmembrane helix 8 from 147 (out) to 162 (in) : 1784.06 Transmembrane helix 9 from 166 (in) to 181 (out) : 549.899 Transmembrane helix 10 from 185 (out) to 200 (in) : 459.338 Transmembrane helix 11 from 246 (in) to 261 (out) : 2118.07 Transmembrane helix 12 from 265 (out) to 280 (in) : 503.168 Transmembrane helix 13 from 284 (in) to 304 (out) : 2540.05 Transmembrane helix 14 from 311 (out) to 330 (in) : 2957.12 Transmembrane helix 15 from 335 (in) to 356 (out) : 2776.27 Transmembrane helix 16 from 360 (out) to 375 (in) : 115.275 Transmembrane helix 17 from 379 (in) to 394 (out) : 944.4 Transmembrane helix 18 from 401 (out) to 420 (in) : 2750.6 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 17 (out) to 33 (in) : 1760.74 Transmembrane helix 2 from 37 (in) to 52 (out) : 126.152 Transmembrane helix 3 from 57 (out) to 74 (in) : 1109.2 Transmembrane helix 4 from 84 (in) to 103 (out) : 1099.86 Transmembrane helix 5 from 108 (out) to 123 (in) : 627.993 Transmembrane helix 6 from 127 (in) to 142 (out) : -115.015 Transmembrane helix 7 from 146 (out) to 162 (in) : 1829.89 Transmembrane helix 8 from 166 (in) to 181 (out) : 549.899 Transmembrane helix 9 from 185 (out) to 200 (in) : 459.338 Transmembrane helix 10 from 246 (in) to 261 (out) : 2118.07 Transmembrane helix 11 from 265 (out) to 280 (in) : 503.168 Transmembrane helix 12 from 284 (in) to 304 (out) : 2540.05 Transmembrane helix 13 from 311 (out) to 330 (in) : 2957.12 Transmembrane helix 14 from 335 (in) to 356 (out) : 2776.27 Transmembrane helix 15 from 360 (out) to 375 (in) : 115.275 Transmembrane helix 16 from 379 (in) to 394 (out) : 944.4 Transmembrane helix 17 from 401 (out) to 420 (in) : 2750.6 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 17 (in) to 33 (out) : 1772.86 Transmembrane helix 2 from 37 (out) to 52 (in) : 132.348 Transmembrane helix 3 from 56 (in) to 74 (out) : 1092.98 Transmembrane helix 4 from 84 (out) to 103 (in) : 1062.94 Transmembrane helix 5 from 107 (in) to 122 (out) : 558.526 Transmembrane helix 6 from 126 (out) to 141 (in) : -87.836 Transmembrane helix 7 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 8 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 9 from 246 (in) to 261 (out) : 2118.07 Transmembrane helix 10 from 265 (out) to 280 (in) : 503.168 Transmembrane helix 11 from 284 (in) to 304 (out) : 2540.05 Transmembrane helix 12 from 311 (out) to 330 (in) : 2957.12 Transmembrane helix 13 from 335 (in) to 356 (out) : 2776.27 Transmembrane helix 14 from 360 (out) to 375 (in) : 115.275 Transmembrane helix 15 from 379 (in) to 394 (out) : 944.4 Transmembrane helix 16 from 401 (out) to 420 (in) : 2750.6 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 17 (out) to 33 (in) : 1760.74 Transmembrane helix 2 from 37 (in) to 52 (out) : 126.152 Transmembrane helix 3 from 57 (out) to 74 (in) : 1109.2 Transmembrane helix 4 from 84 (in) to 103 (out) : 1099.86 Transmembrane helix 5 from 109 (out) to 129 (in) : 779.736 Transmembrane helix 6 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 7 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 8 from 246 (in) to 261 (out) : 2118.07 Transmembrane helix 9 from 265 (out) to 280 (in) : 503.168 Transmembrane helix 10 from 284 (in) to 304 (out) : 2540.05 Transmembrane helix 11 from 311 (out) to 330 (in) : 2957.12 Transmembrane helix 12 from 335 (in) to 356 (out) : 2776.27 Transmembrane helix 13 from 360 (out) to 375 (in) : 115.275 Transmembrane helix 14 from 379 (in) to 394 (out) : 944.4 Transmembrane helix 15 from 401 (out) to 420 (in) : 2750.6 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 17 (in) to 42 (out) : 2240.41 Transmembrane helix 2 from 57 (out) to 74 (in) : 1109.2 Transmembrane helix 3 from 84 (in) to 103 (out) : 1099.86 Transmembrane helix 4 from 109 (out) to 129 (in) : 779.736 Transmembrane helix 5 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 6 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 7 from 246 (in) to 261 (out) : 2118.07 Transmembrane helix 8 from 265 (out) to 280 (in) : 503.168 Transmembrane helix 9 from 284 (in) to 304 (out) : 2540.05 Transmembrane helix 10 from 311 (out) to 330 (in) : 2957.12 Transmembrane helix 11 from 335 (in) to 356 (out) : 2776.27 Transmembrane helix 12 from 360 (out) to 375 (in) : 115.275 Transmembrane helix 13 from 379 (in) to 394 (out) : 944.4 Transmembrane helix 14 from 401 (out) to 420 (in) : 2750.6 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 17 (out) to 33 (in) : 1760.74 Transmembrane helix 2 from 37 (in) to 52 (out) : 126.152 Transmembrane helix 3 from 57 (out) to 74 (in) : 1109.2 Transmembrane helix 4 from 84 (in) to 103 (out) : 1099.86 Transmembrane helix 5 from 109 (out) to 129 (in) : 779.736 Transmembrane helix 6 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 7 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 8 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 9 from 283 (out) to 304 (in) : 2608.84 Transmembrane helix 10 from 310 (in) to 330 (out) : 3011.55 Transmembrane helix 11 from 335 (out) to 361 (in) : 2830.03 Transmembrane helix 12 from 370 (in) to 393 (out) : 1176.98 Transmembrane helix 13 from 401 (out) to 420 (in) : 2750.6 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 17 (in) to 42 (out) : 2240.41 Transmembrane helix 2 from 57 (out) to 74 (in) : 1109.2 Transmembrane helix 3 from 84 (in) to 103 (out) : 1099.86 Transmembrane helix 4 from 109 (out) to 129 (in) : 779.736 Transmembrane helix 5 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 6 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 7 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 8 from 283 (out) to 304 (in) : 2608.84 Transmembrane helix 9 from 310 (in) to 330 (out) : 3011.55 Transmembrane helix 10 from 335 (out) to 361 (in) : 2830.03 Transmembrane helix 11 from 370 (in) to 393 (out) : 1176.98 Transmembrane helix 12 from 401 (out) to 420 (in) : 2750.6 Score = 24.204027 Processing 11 helices: Transmembrane helix 1 from 17 (out) to 42 (in) : 2200.39 Transmembrane helix 2 from 57 (in) to 87 (out) : 1126.56 Transmembrane helix 3 from 91 (out) to 121 (in) : 1315.65 Transmembrane helix 4 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 5 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 6 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 7 from 283 (out) to 304 (in) : 2608.84 Transmembrane helix 8 from 310 (in) to 330 (out) : 3011.55 Transmembrane helix 9 from 335 (out) to 361 (in) : 2830.03 Transmembrane helix 10 from 370 (in) to 393 (out) : 1176.98 Transmembrane helix 11 from 401 (out) to 420 (in) : 2750.6 Score = 23.512455 Processing 10 helices: Transmembrane helix 1 from 17 (in) to 42 (out) : 2240.41 Transmembrane helix 2 from 88 (out) to 118 (in) : 1353.57 Transmembrane helix 3 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 4 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 5 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 6 from 283 (out) to 304 (in) : 2608.84 Transmembrane helix 7 from 310 (in) to 330 (out) : 3011.55 Transmembrane helix 8 from 335 (out) to 361 (in) : 2830.03 Transmembrane helix 9 from 370 (in) to 393 (out) : 1176.98 Transmembrane helix 10 from 401 (out) to 420 (in) : 2750.6 Score = 22.531696 Processing 9 helices: Transmembrane helix 1 from 17 (out) to 42 (in) : 2200.39 Transmembrane helix 2 from 57 (in) to 87 (out) : 1126.56 Transmembrane helix 3 from 91 (out) to 121 (in) : 1315.65 Transmembrane helix 4 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 5 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 6 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 7 from 284 (out) to 314 (in) : 2924.13 Transmembrane helix 8 from 318 (in) to 348 (out) : 4519.2 Transmembrane helix 9 from 387 (out) to 417 (in) : 3018.53 Score = 21.613712 Processing 8 helices: Transmembrane helix 1 from 17 (in) to 42 (out) : 2240.41 Transmembrane helix 2 from 88 (out) to 118 (in) : 1353.57 Transmembrane helix 3 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 4 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 5 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 6 from 284 (out) to 314 (in) : 2924.13 Transmembrane helix 7 from 318 (in) to 348 (out) : 4519.2 Transmembrane helix 8 from 387 (out) to 417 (in) : 3018.53 Score = 20.632956 Processing 7 helices: Transmembrane helix 1 from 17 (out) to 42 (in) : 2200.39 Transmembrane helix 2 from 145 (in) to 166 (out) : 2020.08 Transmembrane helix 3 from 174 (out) to 194 (in) : 1351.19 Transmembrane helix 4 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 5 from 284 (out) to 314 (in) : 2924.13 Transmembrane helix 6 from 318 (in) to 348 (out) : 4519.2 Transmembrane helix 7 from 387 (out) to 417 (in) : 3018.53 Score = 19.168524 Processing 6 helices: Transmembrane helix 1 from 17 (in) to 42 (out) : 2240.41 Transmembrane helix 2 from 150 (out) to 180 (in) : 2105.93 Transmembrane helix 3 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 4 from 284 (out) to 314 (in) : 2924.13 Transmembrane helix 5 from 318 (in) to 348 (out) : 4519.2 Transmembrane helix 6 from 387 (out) to 417 (in) : 3018.53 Score = 17.890417 Processing 5 helices: Transmembrane helix 1 from 17 (out) to 42 (in) : 2200.39 Transmembrane helix 2 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 3 from 284 (out) to 314 (in) : 2924.13 Transmembrane helix 4 from 318 (in) to 348 (out) : 4519.2 Transmembrane helix 5 from 387 (out) to 417 (in) : 3018.53 Score = 15.698155 Processing 4 helices: Transmembrane helix 1 from 247 (in) to 274 (out) : 2956.43 Transmembrane helix 2 from 284 (out) to 314 (in) : 2924.13 Transmembrane helix 3 from 318 (in) to 348 (out) : 4519.2 Transmembrane helix 4 from 387 (out) to 417 (in) : 3018.53 Score = 13.520923 Processing 3 helices: Transmembrane helix 1 from 247 (out) to 274 (in) : 2877.37 Transmembrane helix 2 from 316 (in) to 346 (out) : 4645.68 Transmembrane helix 3 from 387 (out) to 417 (in) : 3018.53 Score = 10.582636 Processing 2 helices: Transmembrane helix 1 from 316 (in) to 346 (out) : 4645.68 Transmembrane helix 2 from 387 (out) to 417 (in) : 3018.53 Score = 7.744303 Summary of topology analysis: 1 helix (+) : Score = 4.64568 1 helix (-) : Score = 4.59382 2 helices (+) : Score = 7.7443 2 helices (-) : Score = 7.5074 3 helices (+) : Score = 10.5029 3 helices (-) : Score = 10.5826 4 helices (+) : Score = 13.5209 4 helices (-) : Score = 13.0973 5 helices (+) : Score = 15.3609 5 helices (-) : Score = 15.6982 6 helices (+) : Score = 17.8904 6 helices (-) : Score = 17.39 7 helices (+) : Score = 18.8546 7 helices (-) : Score = 19.1685 8 helices (+) : Score = 20.633 8 helices (-) : Score = 20.0624 9 helices (+) : Score = 21.5269 9 helices (-) : Score = 21.6137 10 helices (+) : Score = 22.5317 10 helices (-) : Score = 22.5076 11 helices (+) : Score = 23.1992 11 helices (-) : Score = 23.5125 12 helices (+) : Score = 24.204 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 22 helices (+) : Score = -100000 22 helices (-) : Score = -100000 23 helices (+) : Score = -100000 23 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 17-42,57-74,84-103,109-129,145-166,174-194,247-274,283-304,310-330,335-361,370-393,401-420 Re-entrant helices: Not detected. Helix count: 12 N-terminal: in Score: 24.204