BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (84 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P0AB34 Biofilm regulator bssS n=144 Tax=Enterobacteriac...   177   1e-43

>UniRef50_P0AB34 Biofilm regulator bssS n=144 Tax=Enterobacteriaceae
          RepID=BSSS_ECOL6
          Length = 84

 Score =  177 bits (448), Expect = 1e-43,   Method: Compositional matrix adjust.
 Identities = 84/84 (100%), Positives = 84/84 (100%)

Query: 1  MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGTEVGQTLWLTTDVARQF 60
          MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGTEVGQTLWLTTDVARQF
Sbjct: 1  MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGTEVGQTLWLTTDVARQF 60

Query: 61 ISILEAGIAKIESGDFQVNEYRRH 84
          ISILEAGIAKIESGDFQVNEYRRH
Sbjct: 61 ISILEAGIAKIESGDFQVNEYRRH 84


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P0AB34 Biofilm regulator bssS n=144 Tax=Enterobacteriac...   181   9e-45

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P0AB34 Biofilm regulator bssS n=144 Tax=Enterobacteriaceae
          RepID=BSSS_ECOL6
          Length = 84

 Score =  181 bits (458), Expect = 9e-45,   Method: Composition-based stats.
 Identities = 84/84 (100%), Positives = 84/84 (100%)

Query: 1  MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGTEVGQTLWLTTDVARQF 60
          MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGTEVGQTLWLTTDVARQF
Sbjct: 1  MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGTEVGQTLWLTTDVARQF 60

Query: 61 ISILEAGIAKIESGDFQVNEYRRH 84
          ISILEAGIAKIESGDFQVNEYRRH
Sbjct: 61 ISILEAGIAKIESGDFQVNEYRRH 84


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.315    0.131    0.383 

Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 322,422,019
Number of Sequences: 3077464
Number of extensions: 10006449
Number of successful extensions: 20318
Number of sequences better than 1.0e-01: 1
Number of HSP's better than  0.1 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 20316
Number of HSP's gapped (non-prelim): 2
length of query: 84
length of database: 1,040,396,356
effective HSP length: 54
effective length of query: 30
effective length of database: 874,213,300
effective search space: 26226399000
effective search space used: 26226399000
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 87 (38.1 bits)