Processing 1 helix: Transmembrane helix 1 from 47 (in) to 75 (out) : 709.394 Score = 0.727051 Processing 1 helix: Transmembrane helix 1 from 47 (out) to 75 (in) : 705.806 Score = 0.688149 Processing 22 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -811.324 Transmembrane helix 2 from 23 (out) to 38 (in) : -828.82 Transmembrane helix 3 from 42 (in) to 57 (out) : 186.673 Transmembrane helix 4 from 61 (out) to 76 (in) : 435.645 Transmembrane helix 5 from 80 (in) to 95 (out) : -1387.74 Transmembrane helix 6 from 99 (out) to 114 (in) : -588.058 Transmembrane helix 7 from 118 (in) to 133 (out) : -679.436 Transmembrane helix 8 from 137 (out) to 152 (in) : -523.635 Transmembrane helix 9 from 156 (in) to 171 (out) : -848.077 Transmembrane helix 10 from 175 (out) to 190 (in) : 27.372 Transmembrane helix 11 from 194 (in) to 209 (out) : -754.456 Transmembrane helix 12 from 213 (out) to 228 (in) : -656.281 Transmembrane helix 13 from 232 (in) to 247 (out) : -882.068 Transmembrane helix 14 from 251 (out) to 266 (in) : -846.45 Transmembrane helix 15 from 270 (in) to 285 (out) : -1036.19 Transmembrane helix 16 from 291 (out) to 306 (in) : -1132.26 Transmembrane helix 17 from 310 (in) to 325 (out) : -873.458 Transmembrane helix 18 from 329 (out) to 344 (in) : -1082.62 Transmembrane helix 19 from 348 (in) to 363 (out) : -649.341 Transmembrane helix 20 from 367 (out) to 382 (in) : -965.717 Transmembrane helix 21 from 386 (in) to 401 (out) : -971.359 Transmembrane helix 22 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -801.176 Transmembrane helix 2 from 28 (in) to 43 (out) : -682.248 Transmembrane helix 3 from 47 (out) to 62 (in) : 368.766 Transmembrane helix 4 from 66 (in) to 81 (out) : 113.171 Transmembrane helix 5 from 97 (out) to 112 (in) : -609.994 Transmembrane helix 6 from 116 (in) to 131 (out) : -741.413 Transmembrane helix 7 from 135 (out) to 150 (in) : -428.665 Transmembrane helix 8 from 156 (in) to 171 (out) : -848.077 Transmembrane helix 9 from 175 (out) to 190 (in) : 27.372 Transmembrane helix 10 from 194 (in) to 209 (out) : -754.456 Transmembrane helix 11 from 213 (out) to 228 (in) : -656.281 Transmembrane helix 12 from 232 (in) to 247 (out) : -882.068 Transmembrane helix 13 from 251 (out) to 266 (in) : -846.45 Transmembrane helix 14 from 270 (in) to 285 (out) : -1036.19 Transmembrane helix 15 from 291 (out) to 306 (in) : -1132.26 Transmembrane helix 16 from 310 (in) to 325 (out) : -873.458 Transmembrane helix 17 from 329 (out) to 344 (in) : -1082.62 Transmembrane helix 18 from 348 (in) to 363 (out) : -649.341 Transmembrane helix 19 from 367 (out) to 382 (in) : -965.717 Transmembrane helix 20 from 386 (in) to 401 (out) : -971.359 Transmembrane helix 21 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : -812.732 Transmembrane helix 2 from 28 (out) to 43 (in) : -676.652 Transmembrane helix 3 from 47 (in) to 62 (out) : 375.834 Transmembrane helix 4 from 66 (out) to 81 (in) : 120.229 Transmembrane helix 5 from 98 (in) to 113 (out) : -574.046 Transmembrane helix 6 from 117 (out) to 132 (in) : -713.14 Transmembrane helix 7 from 136 (in) to 151 (out) : -492.899 Transmembrane helix 8 from 155 (out) to 170 (in) : -925.709 Transmembrane helix 9 from 174 (in) to 189 (out) : 70.038 Transmembrane helix 10 from 193 (out) to 208 (in) : -733.069 Transmembrane helix 11 from 212 (in) to 227 (out) : -679.89 Transmembrane helix 12 from 231 (out) to 246 (in) : -893.277 Transmembrane helix 13 from 250 (in) to 265 (out) : -856.319 Transmembrane helix 14 from 269 (out) to 284 (in) : -989.11 Transmembrane helix 15 from 296 (in) to 311 (out) : -952.721 Transmembrane helix 16 from 315 (out) to 330 (in) : -989.743 Transmembrane helix 17 from 339 (in) to 354 (out) : -776.966 Transmembrane helix 18 from 358 (out) to 373 (in) : -701.485 Transmembrane helix 19 from 386 (in) to 401 (out) : -971.359 Transmembrane helix 20 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -801.176 Transmembrane helix 2 from 28 (in) to 43 (out) : -682.248 Transmembrane helix 3 from 47 (out) to 62 (in) : 368.766 Transmembrane helix 4 from 66 (in) to 81 (out) : 113.171 Transmembrane helix 5 from 97 (out) to 112 (in) : -609.994 Transmembrane helix 6 from 116 (in) to 131 (out) : -741.413 Transmembrane helix 7 from 135 (out) to 150 (in) : -428.665 Transmembrane helix 8 from 166 (in) to 181 (out) : 11.308 Transmembrane helix 9 from 185 (out) to 200 (in) : -548.341 Transmembrane helix 10 from 204 (in) to 219 (out) : -824.723 Transmembrane helix 11 from 223 (out) to 238 (in) : -704.6 Transmembrane helix 12 from 242 (in) to 257 (out) : -886.959 Transmembrane helix 13 from 261 (out) to 276 (in) : -788.164 Transmembrane helix 14 from 296 (in) to 311 (out) : -952.721 Transmembrane helix 15 from 315 (out) to 330 (in) : -989.743 Transmembrane helix 16 from 339 (in) to 354 (out) : -776.966 Transmembrane helix 17 from 358 (out) to 373 (in) : -701.485 Transmembrane helix 18 from 386 (in) to 401 (out) : -971.359 Transmembrane helix 19 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : -812.732 Transmembrane helix 2 from 28 (out) to 43 (in) : -676.652 Transmembrane helix 3 from 47 (in) to 62 (out) : 375.834 Transmembrane helix 4 from 66 (out) to 81 (in) : 120.229 Transmembrane helix 5 from 98 (in) to 113 (out) : -574.046 Transmembrane helix 6 from 117 (out) to 132 (in) : -713.14 Transmembrane helix 7 from 136 (in) to 151 (out) : -492.899 Transmembrane helix 8 from 166 (out) to 181 (in) : -2.50801 Transmembrane helix 9 from 185 (in) to 200 (out) : -561.859 Transmembrane helix 10 from 204 (out) to 219 (in) : -825.377 Transmembrane helix 11 from 223 (in) to 238 (out) : -709.3 Transmembrane helix 12 from 242 (out) to 257 (in) : -883.541 Transmembrane helix 13 from 261 (in) to 276 (out) : -801.636 Transmembrane helix 14 from 302 (out) to 317 (in) : -853.694 Transmembrane helix 15 from 339 (in) to 354 (out) : -776.966 Transmembrane helix 16 from 358 (out) to 373 (in) : -701.485 Transmembrane helix 17 from 386 (in) to 401 (out) : -971.359 Transmembrane helix 18 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -801.176 Transmembrane helix 2 from 28 (in) to 43 (out) : -682.248 Transmembrane helix 3 from 47 (out) to 62 (in) : 368.766 Transmembrane helix 4 from 66 (in) to 81 (out) : 113.171 Transmembrane helix 5 from 97 (out) to 112 (in) : -609.994 Transmembrane helix 6 from 116 (in) to 131 (out) : -741.413 Transmembrane helix 7 from 135 (out) to 150 (in) : -428.665 Transmembrane helix 8 from 166 (in) to 181 (out) : 11.308 Transmembrane helix 9 from 185 (out) to 200 (in) : -548.341 Transmembrane helix 10 from 204 (in) to 219 (out) : -824.723 Transmembrane helix 11 from 223 (out) to 238 (in) : -704.6 Transmembrane helix 12 from 242 (in) to 257 (out) : -886.959 Transmembrane helix 13 from 261 (out) to 276 (in) : -788.164 Transmembrane helix 14 from 303 (in) to 318 (out) : -852.861 Transmembrane helix 15 from 339 (out) to 354 (in) : -775.534 Transmembrane helix 16 from 358 (in) to 373 (out) : -710.115 Transmembrane helix 17 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : -812.732 Transmembrane helix 2 from 28 (out) to 43 (in) : -676.652 Transmembrane helix 3 from 47 (in) to 62 (out) : 375.834 Transmembrane helix 4 from 66 (out) to 81 (in) : 120.229 Transmembrane helix 5 from 98 (in) to 113 (out) : -574.046 Transmembrane helix 6 from 117 (out) to 132 (in) : -713.14 Transmembrane helix 7 from 136 (in) to 151 (out) : -492.899 Transmembrane helix 8 from 167 (out) to 182 (in) : 38.224 Transmembrane helix 9 from 186 (in) to 201 (out) : -599.6 Transmembrane helix 10 from 215 (out) to 230 (in) : -614.644 Transmembrane helix 11 from 234 (in) to 249 (out) : -901.705 Transmembrane helix 12 from 257 (out) to 272 (in) : -750.951 Transmembrane helix 13 from 303 (in) to 318 (out) : -852.861 Transmembrane helix 14 from 339 (out) to 354 (in) : -775.534 Transmembrane helix 15 from 358 (in) to 373 (out) : -710.115 Transmembrane helix 16 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -801.176 Transmembrane helix 2 from 28 (in) to 43 (out) : -682.248 Transmembrane helix 3 from 47 (out) to 62 (in) : 368.766 Transmembrane helix 4 from 66 (in) to 81 (out) : 113.171 Transmembrane helix 5 from 97 (out) to 112 (in) : -609.994 Transmembrane helix 6 from 116 (in) to 131 (out) : -741.413 Transmembrane helix 7 from 135 (out) to 150 (in) : -428.665 Transmembrane helix 8 from 167 (in) to 182 (out) : 50.976 Transmembrane helix 9 from 186 (out) to 201 (in) : -585.7 Transmembrane helix 10 from 219 (in) to 234 (out) : -604.317 Transmembrane helix 11 from 257 (out) to 272 (in) : -750.951 Transmembrane helix 12 from 303 (in) to 318 (out) : -852.861 Transmembrane helix 13 from 339 (out) to 354 (in) : -775.534 Transmembrane helix 14 from 358 (in) to 373 (out) : -710.115 Transmembrane helix 15 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : -812.732 Transmembrane helix 2 from 28 (out) to 43 (in) : -676.652 Transmembrane helix 3 from 47 (in) to 62 (out) : 375.834 Transmembrane helix 4 from 66 (out) to 81 (in) : 120.229 Transmembrane helix 5 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 6 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 7 from 167 (in) to 182 (out) : 50.976 Transmembrane helix 8 from 186 (out) to 201 (in) : -585.7 Transmembrane helix 9 from 219 (in) to 234 (out) : -604.317 Transmembrane helix 10 from 257 (out) to 272 (in) : -750.951 Transmembrane helix 11 from 303 (in) to 318 (out) : -852.861 Transmembrane helix 12 from 339 (out) to 354 (in) : -775.534 Transmembrane helix 13 from 358 (in) to 373 (out) : -710.115 Transmembrane helix 14 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -801.176 Transmembrane helix 2 from 41 (in) to 56 (out) : 165.803 Transmembrane helix 3 from 60 (out) to 75 (in) : 458.866 Transmembrane helix 4 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 5 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 6 from 167 (in) to 182 (out) : 50.976 Transmembrane helix 7 from 186 (out) to 201 (in) : -585.7 Transmembrane helix 8 from 219 (in) to 234 (out) : -604.317 Transmembrane helix 9 from 257 (out) to 272 (in) : -750.951 Transmembrane helix 10 from 303 (in) to 318 (out) : -852.861 Transmembrane helix 11 from 339 (out) to 354 (in) : -775.534 Transmembrane helix 12 from 358 (in) to 373 (out) : -710.115 Transmembrane helix 13 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : -812.732 Transmembrane helix 2 from 28 (out) to 43 (in) : -676.652 Transmembrane helix 3 from 47 (in) to 62 (out) : 375.834 Transmembrane helix 4 from 66 (out) to 81 (in) : 120.229 Transmembrane helix 5 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 6 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 7 from 167 (in) to 182 (out) : 50.976 Transmembrane helix 8 from 186 (out) to 201 (in) : -585.7 Transmembrane helix 9 from 219 (in) to 234 (out) : -604.317 Transmembrane helix 10 from 257 (out) to 272 (in) : -750.951 Transmembrane helix 11 from 349 (in) to 364 (out) : -636.363 Transmembrane helix 12 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -801.176 Transmembrane helix 2 from 41 (in) to 56 (out) : 165.803 Transmembrane helix 3 from 60 (out) to 75 (in) : 458.866 Transmembrane helix 4 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 5 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 6 from 167 (in) to 182 (out) : 50.976 Transmembrane helix 7 from 186 (out) to 201 (in) : -585.7 Transmembrane helix 8 from 219 (in) to 234 (out) : -604.317 Transmembrane helix 9 from 257 (out) to 272 (in) : -750.951 Transmembrane helix 10 from 349 (in) to 364 (out) : -636.363 Transmembrane helix 11 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : 165.803 Transmembrane helix 2 from 60 (out) to 75 (in) : 458.866 Transmembrane helix 3 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 4 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 5 from 167 (in) to 182 (out) : 50.976 Transmembrane helix 6 from 186 (out) to 201 (in) : -585.7 Transmembrane helix 7 from 219 (in) to 234 (out) : -604.317 Transmembrane helix 8 from 257 (out) to 272 (in) : -750.951 Transmembrane helix 9 from 349 (in) to 364 (out) : -636.363 Transmembrane helix 10 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -801.176 Transmembrane helix 2 from 41 (in) to 56 (out) : 165.803 Transmembrane helix 3 from 60 (out) to 75 (in) : 458.866 Transmembrane helix 4 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 5 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 6 from 171 (in) to 186 (out) : 144.254 Transmembrane helix 7 from 219 (out) to 234 (in) : -600.883 Transmembrane helix 8 from 349 (in) to 364 (out) : -636.363 Transmembrane helix 9 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : 165.803 Transmembrane helix 2 from 60 (out) to 75 (in) : 458.866 Transmembrane helix 3 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 4 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 5 from 171 (in) to 186 (out) : 144.254 Transmembrane helix 6 from 219 (out) to 234 (in) : -600.883 Transmembrane helix 7 from 349 (in) to 364 (out) : -636.363 Transmembrane helix 8 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : 152.397 Transmembrane helix 2 from 60 (in) to 75 (out) : 451.734 Transmembrane helix 3 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 4 from 171 (in) to 186 (out) : 144.254 Transmembrane helix 5 from 219 (out) to 234 (in) : -600.883 Transmembrane helix 6 from 349 (in) to 364 (out) : -636.363 Transmembrane helix 7 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : 165.803 Transmembrane helix 2 from 60 (out) to 75 (in) : 458.866 Transmembrane helix 3 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 4 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 5 from 171 (in) to 186 (out) : 144.254 Transmembrane helix 6 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : 152.397 Transmembrane helix 2 from 60 (in) to 75 (out) : 451.734 Transmembrane helix 3 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 4 from 171 (in) to 186 (out) : 144.254 Transmembrane helix 5 from 405 (out) to 420 (in) : -452.199 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : 165.803 Transmembrane helix 2 from 60 (out) to 75 (in) : 458.866 Transmembrane helix 3 from 130 (in) to 145 (out) : -350.079 Transmembrane helix 4 from 171 (out) to 186 (in) : 138.746 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : 152.397 Transmembrane helix 2 from 60 (in) to 75 (out) : 451.734 Transmembrane helix 3 from 171 (out) to 186 (in) : 138.746 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 47 (in) to 75 (out) : 709.394 Transmembrane helix 2 from 171 (out) to 186 (in) : 138.746 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -801.176 Transmembrane helix 2 from 23 (in) to 38 (out) : -819.28 Transmembrane helix 3 from 42 (out) to 57 (in) : 172.127 Transmembrane helix 4 from 61 (in) to 76 (out) : 427.755 Transmembrane helix 5 from 80 (out) to 95 (in) : -1388.56 Transmembrane helix 6 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 7 from 118 (out) to 133 (in) : -675.264 Transmembrane helix 8 from 137 (in) to 152 (out) : -542.165 Transmembrane helix 9 from 156 (out) to 171 (in) : -854.523 Transmembrane helix 10 from 175 (in) to 190 (out) : 28.828 Transmembrane helix 11 from 194 (out) to 209 (in) : -749.544 Transmembrane helix 12 from 213 (in) to 228 (out) : -657.919 Transmembrane helix 13 from 232 (out) to 247 (in) : -905.832 Transmembrane helix 14 from 251 (in) to 266 (out) : -845.75 Transmembrane helix 15 from 270 (out) to 285 (in) : -1024.41 Transmembrane helix 16 from 291 (in) to 306 (out) : -1131.94 Transmembrane helix 17 from 310 (out) to 325 (in) : -873.542 Transmembrane helix 18 from 329 (in) to 344 (out) : -1071.78 Transmembrane helix 19 from 348 (out) to 363 (in) : -643.459 Transmembrane helix 20 from 367 (in) to 382 (out) : -965.583 Transmembrane helix 21 from 386 (out) to 401 (in) : -979.741 Transmembrane helix 22 from 405 (in) to 420 (out) : -462.901 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : -812.732 Transmembrane helix 2 from 28 (out) to 43 (in) : -676.652 Transmembrane helix 3 from 47 (in) to 62 (out) : 375.834 Transmembrane helix 4 from 66 (out) to 81 (in) : 120.229 Transmembrane helix 5 from 98 (in) to 113 (out) : -574.046 Transmembrane helix 6 from 117 (out) to 132 (in) : -713.14 Transmembrane helix 7 from 136 (in) to 151 (out) : -492.899 Transmembrane helix 8 from 155 (out) to 170 (in) : -925.709 Transmembrane helix 9 from 174 (in) to 189 (out) : 70.038 Transmembrane helix 10 from 193 (out) to 208 (in) : -733.069 Transmembrane helix 11 from 212 (in) to 227 (out) : -679.89 Transmembrane helix 12 from 231 (out) to 246 (in) : -893.277 Transmembrane helix 13 from 250 (in) to 265 (out) : -856.319 Transmembrane helix 14 from 269 (out) to 284 (in) : -989.11 Transmembrane helix 15 from 291 (in) to 306 (out) : -1131.94 Transmembrane helix 16 from 310 (out) to 325 (in) : -873.542 Transmembrane helix 17 from 329 (in) to 344 (out) : -1071.78 Transmembrane helix 18 from 348 (out) to 363 (in) : -643.459 Transmembrane helix 19 from 367 (in) to 382 (out) : -965.583 Transmembrane helix 20 from 386 (out) to 401 (in) : -979.741 Transmembrane helix 21 from 405 (in) to 420 (out) : -462.901 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -801.176 Transmembrane helix 2 from 28 (in) to 43 (out) : -682.248 Transmembrane helix 3 from 47 (out) to 62 (in) : 368.766 Transmembrane helix 4 from 66 (in) to 81 (out) : 113.171 Transmembrane helix 5 from 97 (out) to 112 (in) : -609.994 Transmembrane helix 6 from 116 (in) to 131 (out) : -741.413 Transmembrane helix 7 from 135 (out) to 150 (in) : -428.665 Transmembrane helix 8 from 166 (in) to 181 (out) : 11.308 Transmembrane helix 9 from 185 (out) to 200 (in) : -548.341 Transmembrane helix 10 from 204 (in) to 219 (out) : -824.723 Transmembrane helix 11 from 223 (out) to 238 (in) : -704.6 Transmembrane helix 12 from 242 (in) to 257 (out) : -886.959 Transmembrane helix 13 from 261 (out) to 276 (in) : -788.164 Transmembrane helix 14 from 291 (in) to 306 (out) : -1131.94 Transmembrane helix 15 from 310 (out) to 325 (in) : -873.542 Transmembrane helix 16 from 329 (in) to 344 (out) : -1071.78 Transmembrane helix 17 from 348 (out) to 363 (in) : -643.459 Transmembrane helix 18 from 367 (in) to 382 (out) : -965.583 Transmembrane helix 19 from 386 (out) to 401 (in) : -979.741 Transmembrane helix 20 from 405 (in) to 420 (out) : -462.901 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : -812.732 Transmembrane helix 2 from 28 (out) to 43 (in) : -676.652 Transmembrane helix 3 from 47 (in) to 62 (out) : 375.834 Transmembrane helix 4 from 66 (out) to 81 (in) : 120.229 Transmembrane helix 5 from 98 (in) to 113 (out) : -574.046 Transmembrane helix 6 from 117 (out) to 132 (in) : -713.14 Transmembrane helix 7 from 136 (in) to 151 (out) : -492.899 Transmembrane helix 8 from 166 (out) to 181 (in) : -2.50801 Transmembrane helix 9 from 185 (in) to 200 (out) : -561.859 Transmembrane helix 10 from 204 (out) to 219 (in) : -825.377 Transmembrane helix 11 from 223 (in) to 238 (out) : -709.3 Transmembrane helix 12 from 242 (out) to 257 (in) : -883.541 Transmembrane helix 13 from 261 (in) to 276 (out) : -801.636 Transmembrane helix 14 from 295 (out) to 310 (in) : -979.641 Transmembrane helix 15 from 314 (in) to 329 (out) : -955.57 Transmembrane helix 16 from 339 (out) to 354 (in) : -775.534 Transmembrane helix 17 from 358 (in) to 373 (out) : -710.115 Transmembrane helix 18 from 386 (out) to 401 (in) : -979.741 Transmembrane helix 19 from 405 (in) to 420 (out) : -462.901 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -801.176 Transmembrane helix 2 from 28 (in) to 43 (out) : -682.248 Transmembrane helix 3 from 47 (out) to 62 (in) : 368.766 Transmembrane helix 4 from 66 (in) to 81 (out) : 113.171 Transmembrane helix 5 from 97 (out) to 112 (in) : -609.994 Transmembrane helix 6 from 116 (in) to 131 (out) : -741.413 Transmembrane helix 7 from 135 (out) to 150 (in) : -428.665 Transmembrane helix 8 from 166 (in) to 181 (out) : 11.308 Transmembrane helix 9 from 185 (out) to 200 (in) : -548.341 Transmembrane helix 10 from 204 (in) to 219 (out) : -824.723 Transmembrane helix 11 from 223 (out) to 238 (in) : -704.6 Transmembrane helix 12 from 242 (in) to 257 (out) : -886.959 Transmembrane helix 13 from 261 (out) to 276 (in) : -788.164 Transmembrane helix 14 from 303 (in) to 318 (out) : -852.861 Transmembrane helix 15 from 339 (out) to 354 (in) : -775.534 Transmembrane helix 16 from 358 (in) to 373 (out) : -710.115 Transmembrane helix 17 from 386 (out) to 401 (in) : -979.741 Transmembrane helix 18 from 405 (in) to 420 (out) : -462.901 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : -812.732 Transmembrane helix 2 from 28 (out) to 43 (in) : -676.652 Transmembrane helix 3 from 47 (in) to 62 (out) : 375.834 Transmembrane helix 4 from 66 (out) to 81 (in) : 120.229 Transmembrane helix 5 from 98 (in) to 113 (out) : -574.046 Transmembrane helix 6 from 117 (out) to 132 (in) : -713.14 Transmembrane helix 7 from 136 (in) to 151 (out) : -492.899 Transmembrane helix 8 from 167 (out) to 182 (in) : 38.224 Transmembrane helix 9 from 186 (in) to 201 (out) : -599.6 Transmembrane helix 10 from 215 (out) to 230 (in) : -614.644 Transmembrane helix 11 from 234 (in) to 249 (out) : -901.705 Transmembrane helix 12 from 257 (out) to 272 (in) : -750.951 Transmembrane helix 13 from 303 (in) to 318 (out) : -852.861 Transmembrane helix 14 from 339 (out) to 354 (in) : -775.534 Transmembrane helix 15 from 358 (in) to 373 (out) : -710.115 Transmembrane helix 16 from 386 (out) to 401 (in) : -979.741 Transmembrane helix 17 from 405 (in) to 420 (out) : -462.901 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -801.176 Transmembrane helix 2 from 28 (in) to 43 (out) : -682.248 Transmembrane helix 3 from 47 (out) to 62 (in) : 368.766 Transmembrane helix 4 from 66 (in) to 81 (out) : 113.171 Transmembrane helix 5 from 97 (out) to 112 (in) : -609.994 Transmembrane helix 6 from 116 (in) to 131 (out) : -741.413 Transmembrane helix 7 from 135 (out) to 150 (in) : -428.665 Transmembrane helix 8 from 167 (in) to 182 (out) : 50.976 Transmembrane helix 9 from 186 (out) to 201 (in) : -585.7 Transmembrane helix 10 from 219 (in) to 234 (out) : -604.317 Transmembrane helix 11 from 242 (out) to 257 (in) : -883.541 Transmembrane helix 12 from 261 (in) to 276 (out) : -801.636 Transmembrane helix 13 from 302 (out) to 317 (in) : -853.694 Transmembrane helix 14 from 339 (in) to 354 (out) : -776.966 Transmembrane helix 15 from 358 (out) to 373 (in) : -701.485 Transmembrane helix 16 from 404 (in) to 419 (out) : -460.624 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : -812.732 Transmembrane helix 2 from 28 (out) to 43 (in) : -676.652 Transmembrane helix 3 from 47 (in) to 62 (out) : 375.834 Transmembrane helix 4 from 66 (out) to 81 (in) : 120.229 Transmembrane helix 5 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 6 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 7 from 167 (in) to 182 (out) : 50.976 Transmembrane helix 8 from 186 (out) to 201 (in) : -585.7 Transmembrane helix 9 from 219 (in) to 234 (out) : -604.317 Transmembrane helix 10 from 242 (out) to 257 (in) : -883.541 Transmembrane helix 11 from 261 (in) to 276 (out) : -801.636 Transmembrane helix 12 from 302 (out) to 317 (in) : -853.694 Transmembrane helix 13 from 339 (in) to 354 (out) : -776.966 Transmembrane helix 14 from 358 (out) to 373 (in) : -701.485 Transmembrane helix 15 from 404 (in) to 419 (out) : -460.624 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -801.176 Transmembrane helix 2 from 28 (in) to 43 (out) : -682.248 Transmembrane helix 3 from 47 (out) to 62 (in) : 368.766 Transmembrane helix 4 from 66 (in) to 81 (out) : 113.171 Transmembrane helix 5 from 97 (out) to 112 (in) : -609.994 Transmembrane helix 6 from 116 (in) to 131 (out) : -741.413 Transmembrane helix 7 from 135 (out) to 150 (in) : -428.665 Transmembrane helix 8 from 167 (in) to 182 (out) : 50.976 Transmembrane helix 9 from 186 (out) to 201 (in) : -585.7 Transmembrane helix 10 from 219 (in) to 234 (out) : -604.317 Transmembrane helix 11 from 257 (out) to 272 (in) : -750.951 Transmembrane helix 12 from 303 (in) to 318 (out) : -852.861 Transmembrane helix 13 from 349 (out) to 364 (in) : -626.837 Transmembrane helix 14 from 404 (in) to 419 (out) : -460.624 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : -812.732 Transmembrane helix 2 from 28 (out) to 43 (in) : -676.652 Transmembrane helix 3 from 47 (in) to 62 (out) : 375.834 Transmembrane helix 4 from 66 (out) to 81 (in) : 120.229 Transmembrane helix 5 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 6 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 7 from 167 (in) to 182 (out) : 50.976 Transmembrane helix 8 from 186 (out) to 201 (in) : -585.7 Transmembrane helix 9 from 219 (in) to 234 (out) : -604.317 Transmembrane helix 10 from 257 (out) to 272 (in) : -750.951 Transmembrane helix 11 from 303 (in) to 318 (out) : -852.861 Transmembrane helix 12 from 349 (out) to 364 (in) : -626.837 Transmembrane helix 13 from 404 (in) to 419 (out) : -460.624 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -801.176 Transmembrane helix 2 from 41 (in) to 56 (out) : 165.803 Transmembrane helix 3 from 60 (out) to 75 (in) : 458.866 Transmembrane helix 4 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 5 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 6 from 167 (in) to 182 (out) : 50.976 Transmembrane helix 7 from 186 (out) to 201 (in) : -585.7 Transmembrane helix 8 from 219 (in) to 234 (out) : -604.317 Transmembrane helix 9 from 257 (out) to 272 (in) : -750.951 Transmembrane helix 10 from 303 (in) to 318 (out) : -852.861 Transmembrane helix 11 from 349 (out) to 364 (in) : -626.837 Transmembrane helix 12 from 404 (in) to 419 (out) : -460.624 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : 165.803 Transmembrane helix 2 from 60 (out) to 75 (in) : 458.866 Transmembrane helix 3 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 4 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 5 from 167 (in) to 182 (out) : 50.976 Transmembrane helix 6 from 186 (out) to 201 (in) : -585.7 Transmembrane helix 7 from 219 (in) to 234 (out) : -604.317 Transmembrane helix 8 from 257 (out) to 272 (in) : -750.951 Transmembrane helix 9 from 303 (in) to 318 (out) : -852.861 Transmembrane helix 10 from 349 (out) to 364 (in) : -626.837 Transmembrane helix 11 from 404 (in) to 419 (out) : -460.624 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -801.176 Transmembrane helix 2 from 41 (in) to 56 (out) : 165.803 Transmembrane helix 3 from 60 (out) to 75 (in) : 458.866 Transmembrane helix 4 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 5 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 6 from 167 (in) to 182 (out) : 50.976 Transmembrane helix 7 from 186 (out) to 201 (in) : -585.7 Transmembrane helix 8 from 219 (in) to 234 (out) : -604.317 Transmembrane helix 9 from 349 (out) to 364 (in) : -626.837 Transmembrane helix 10 from 404 (in) to 419 (out) : -460.624 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : 165.803 Transmembrane helix 2 from 60 (out) to 75 (in) : 458.866 Transmembrane helix 3 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 4 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 5 from 167 (in) to 182 (out) : 50.976 Transmembrane helix 6 from 186 (out) to 201 (in) : -585.7 Transmembrane helix 7 from 219 (in) to 234 (out) : -604.317 Transmembrane helix 8 from 349 (out) to 364 (in) : -626.837 Transmembrane helix 9 from 404 (in) to 419 (out) : -460.624 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -801.176 Transmembrane helix 2 from 41 (in) to 56 (out) : 165.803 Transmembrane helix 3 from 60 (out) to 75 (in) : 458.866 Transmembrane helix 4 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 5 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 6 from 171 (in) to 186 (out) : 144.254 Transmembrane helix 7 from 219 (out) to 234 (in) : -600.883 Transmembrane helix 8 from 404 (in) to 419 (out) : -460.624 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : 165.803 Transmembrane helix 2 from 60 (out) to 75 (in) : 458.866 Transmembrane helix 3 from 99 (in) to 114 (out) : -572.042 Transmembrane helix 4 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 5 from 171 (in) to 186 (out) : 144.254 Transmembrane helix 6 from 219 (out) to 234 (in) : -600.883 Transmembrane helix 7 from 404 (in) to 419 (out) : -460.624 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : 152.397 Transmembrane helix 2 from 60 (in) to 75 (out) : 451.734 Transmembrane helix 3 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 4 from 171 (in) to 186 (out) : 144.254 Transmembrane helix 5 from 219 (out) to 234 (in) : -600.883 Transmembrane helix 6 from 404 (in) to 419 (out) : -460.624 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : 165.803 Transmembrane helix 2 from 60 (out) to 75 (in) : 458.866 Transmembrane helix 3 from 130 (in) to 145 (out) : -350.079 Transmembrane helix 4 from 171 (out) to 186 (in) : 138.746 Transmembrane helix 5 from 404 (in) to 419 (out) : -460.624 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : 152.397 Transmembrane helix 2 from 60 (in) to 75 (out) : 451.734 Transmembrane helix 3 from 130 (out) to 145 (in) : -339.421 Transmembrane helix 4 from 171 (in) to 186 (out) : 144.254 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : 165.803 Transmembrane helix 2 from 60 (out) to 75 (in) : 458.866 Transmembrane helix 3 from 171 (in) to 186 (out) : 144.254 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 47 (out) to 75 (in) : 705.806 Transmembrane helix 2 from 171 (in) to 186 (out) : 144.254 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.727051 1 helix (-) : Score = 0.688149 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 22 helices (+) : Score = -100000 22 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 47-75 Re-entrant helices: Not detected. Helix count: 1 N-terminal: in Score: 0.727051