BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (57 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_A1AAH4 Uncharacterized protein yciY n=72 Tax=Enterobact...   113   2e-24
UniRef50_D1RS99 Putative uncharacterized protein n=1 Tax=Serrati...    52   4e-06
UniRef50_A7FI49 Putative uncharacterized protein n=10 Tax=Entero...    43   0.004

>UniRef50_A1AAH4 Uncharacterized protein yciY n=72 Tax=Enterobacteriaceae
          RepID=YCIY_ECOK1
          Length = 57

 Score =  113 bits (282), Expect = 2e-24,   Method: Compositional matrix adjust.
 Identities = 57/57 (100%), Positives = 57/57 (100%)

Query: 1  MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILNKQRNSLLFAIYNI 57
          MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILNKQRNSLLFAIYNI
Sbjct: 1  MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILNKQRNSLLFAIYNI 57


>UniRef50_D1RS99 Putative uncharacterized protein n=1 Tax=Serratia odorifera 4Rx13
          RepID=D1RS99_SEROD
          Length = 58

 Score = 52.4 bits (124), Expect = 4e-06,   Method: Compositional matrix adjust.
 Identities = 28/54 (51%), Positives = 38/54 (70%)

Query: 1  MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILNKQRNSLLFAI 54
          M+RSR EVGRWRM RQ+ RR+ RWLE QS  N  I  +R+ + ++ R +LLF +
Sbjct: 1  MRRSRNEVGRWRMLRQSQRRRHRWLERQSCSNRHIIRVRRRLDDQHRRALLFVV 54


>UniRef50_A7FI49 Putative uncharacterized protein n=10 Tax=Enterobacteriaceae
          RepID=A7FI49_YERP3
          Length = 46

 Score = 42.7 bits (99), Expect = 0.004,   Method: Compositional matrix adjust.
 Identities = 21/42 (50%), Positives = 29/42 (69%)

Query: 13 MQRQASRRKSRWLEGQSRRNMRIHSIRKCILNKQRNSLLFAI 54
          M RQ  RRK+RWLEGQSRR   I+ +R+   ++ R +LLF +
Sbjct: 1  MLRQNQRRKTRWLEGQSRRYRHIYCVRQLHEHQHRRALLFTV 42


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_A1AAH4 Uncharacterized protein yciY n=72 Tax=Enterobact...    59   5e-08
UniRef50_D1RS99 Putative uncharacterized protein n=1 Tax=Serrati...    57   2e-07

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_A1AAH4 Uncharacterized protein yciY n=72 Tax=Enterobacteriaceae
          RepID=YCIY_ECOK1
          Length = 57

 Score = 58.9 bits (141), Expect = 5e-08,   Method: Composition-based stats.
 Identities = 57/57 (100%), Positives = 57/57 (100%)

Query: 1  MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILNKQRNSLLFAIYNI 57
          MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILNKQRNSLLFAIYNI
Sbjct: 1  MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILNKQRNSLLFAIYNI 57


>UniRef50_D1RS99 Putative uncharacterized protein n=1 Tax=Serratia odorifera 4Rx13
          RepID=D1RS99_SEROD
          Length = 58

 Score = 57.3 bits (137), Expect = 2e-07,   Method: Composition-based stats.
 Identities = 28/54 (51%), Positives = 38/54 (70%)

Query: 1  MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILNKQRNSLLFAI 54
          M+RSR EVGRWRM RQ+ RR+ RWLE QS  N  I  +R+ + ++ R +LLF +
Sbjct: 1  MRRSRNEVGRWRMLRQSQRRRHRWLERQSCSNRHIIRVRRRLDDQHRRALLFVV 54


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.320    0.139    0.438 

Lambda     K      H
   0.267   0.0426    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 232,839,455
Number of Sequences: 3077464
Number of extensions: 6815225
Number of successful extensions: 49360
Number of sequences better than 1.0e-01: 6
Number of HSP's better than  0.1 without gapping: 9
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 49343
Number of HSP's gapped (non-prelim): 15
length of query: 57
length of database: 1,040,396,356
effective HSP length: 30
effective length of query: 27
effective length of database: 948,072,436
effective search space: 25597955772
effective search space used: 25597955772
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.3 bits)
S2: 87 (38.1 bits)