Processing 1 helix: Transmembrane helix 1 from 145 (in) to 160 (out) : 2.403 Score = -0.002264 Processing 1 helix: Transmembrane helix 1 from 144 (out) to 159 (in) : -4.322 Score = 0.000386 Processing 10 helices: Transmembrane helix 1 from 5 (in) to 20 (out) : -627.189 Transmembrane helix 2 from 24 (out) to 39 (in) : -1066.06 Transmembrane helix 3 from 50 (in) to 65 (out) : -102.87 Transmembrane helix 4 from 69 (out) to 84 (in) : -1075.8 Transmembrane helix 5 from 88 (in) to 103 (out) : -153.158 Transmembrane helix 6 from 107 (out) to 122 (in) : -409.431 Transmembrane helix 7 from 129 (in) to 144 (out) : -493.877 Transmembrane helix 8 from 148 (out) to 163 (in) : -46.374 Transmembrane helix 9 from 167 (in) to 182 (out) : -710.5 Transmembrane helix 10 from 187 (out) to 202 (in) : -381.22 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 8 (out) to 23 (in) : -609.446 Transmembrane helix 2 from 50 (in) to 65 (out) : -102.87 Transmembrane helix 3 from 69 (out) to 84 (in) : -1075.8 Transmembrane helix 4 from 88 (in) to 103 (out) : -153.158 Transmembrane helix 5 from 107 (out) to 122 (in) : -409.431 Transmembrane helix 6 from 129 (in) to 144 (out) : -493.877 Transmembrane helix 7 from 148 (out) to 163 (in) : -46.374 Transmembrane helix 8 from 167 (in) to 182 (out) : -710.5 Transmembrane helix 9 from 187 (out) to 202 (in) : -381.22 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 6 (in) to 21 (out) : -625.334 Transmembrane helix 2 from 49 (out) to 64 (in) : -116.212 Transmembrane helix 3 from 87 (in) to 102 (out) : -163.397 Transmembrane helix 4 from 106 (out) to 121 (in) : -393.265 Transmembrane helix 5 from 129 (in) to 144 (out) : -493.877 Transmembrane helix 6 from 148 (out) to 163 (in) : -46.374 Transmembrane helix 7 from 167 (in) to 182 (out) : -710.5 Transmembrane helix 8 from 187 (out) to 202 (in) : -381.22 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 8 (out) to 23 (in) : -609.446 Transmembrane helix 2 from 50 (in) to 65 (out) : -102.87 Transmembrane helix 3 from 88 (out) to 103 (in) : -134.542 Transmembrane helix 4 from 107 (in) to 122 (out) : -411.369 Transmembrane helix 5 from 133 (out) to 148 (in) : -323.919 Transmembrane helix 6 from 152 (in) to 167 (out) : -137.759 Transmembrane helix 7 from 187 (out) to 202 (in) : -381.22 Re-entrant helix 1 from 171 (out) to 183 (out) : 910 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 50 (in) to 65 (out) : -102.87 Transmembrane helix 2 from 88 (out) to 103 (in) : -134.542 Transmembrane helix 3 from 107 (in) to 122 (out) : -411.369 Transmembrane helix 4 from 133 (out) to 148 (in) : -323.919 Transmembrane helix 5 from 152 (in) to 167 (out) : -137.759 Transmembrane helix 6 from 187 (out) to 202 (in) : -381.22 Re-entrant helix 1 from 171 (out) to 183 (out) : 910 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 49 (out) to 64 (in) : -116.212 Transmembrane helix 2 from 87 (in) to 102 (out) : -163.397 Transmembrane helix 3 from 106 (out) to 121 (in) : -393.265 Transmembrane helix 4 from 145 (in) to 160 (out) : 2.403 Transmembrane helix 5 from 187 (out) to 202 (in) : -381.22 Re-entrant helix 1 from 164 (out) to 183 (out) : 1400 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 50 (in) to 65 (out) : -102.87 Transmembrane helix 2 from 91 (out) to 106 (in) : -121.774 Transmembrane helix 3 from 145 (in) to 160 (out) : 2.403 Transmembrane helix 4 from 187 (out) to 202 (in) : -381.22 Re-entrant helix 1 from 164 (out) to 183 (out) : 1400 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 49 (out) to 64 (in) : -116.212 Transmembrane helix 2 from 92 (in) to 107 (out) : -146.262 Transmembrane helix 3 from 143 (out) to 158 (in) : -4.814 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 50 (in) to 65 (out) : -102.87 Transmembrane helix 2 from 144 (out) to 159 (in) : -4.322 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 5 (out) to 20 (in) : -618.411 Transmembrane helix 2 from 24 (in) to 39 (out) : -1069.34 Transmembrane helix 3 from 49 (out) to 64 (in) : -116.212 Transmembrane helix 4 from 72 (in) to 87 (out) : -1009.18 Transmembrane helix 5 from 91 (out) to 106 (in) : -121.774 Transmembrane helix 6 from 110 (in) to 125 (out) : -503.765 Transmembrane helix 7 from 129 (out) to 144 (in) : -503.523 Transmembrane helix 8 from 148 (in) to 163 (out) : -26.926 Transmembrane helix 9 from 167 (out) to 182 (in) : -713.7 Transmembrane helix 10 from 187 (in) to 202 (out) : -369.38 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 5 (in) to 20 (out) : -627.189 Transmembrane helix 2 from 24 (out) to 39 (in) : -1066.06 Transmembrane helix 3 from 50 (in) to 65 (out) : -102.87 Transmembrane helix 4 from 88 (out) to 103 (in) : -134.542 Transmembrane helix 5 from 107 (in) to 122 (out) : -411.369 Transmembrane helix 6 from 129 (out) to 144 (in) : -503.523 Transmembrane helix 7 from 148 (in) to 163 (out) : -26.926 Transmembrane helix 8 from 167 (out) to 182 (in) : -713.7 Transmembrane helix 9 from 187 (in) to 202 (out) : -369.38 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 8 (out) to 23 (in) : -609.446 Transmembrane helix 2 from 50 (in) to 65 (out) : -102.87 Transmembrane helix 3 from 88 (out) to 103 (in) : -134.542 Transmembrane helix 4 from 107 (in) to 122 (out) : -411.369 Transmembrane helix 5 from 129 (out) to 144 (in) : -503.523 Transmembrane helix 6 from 148 (in) to 163 (out) : -26.926 Transmembrane helix 7 from 167 (out) to 182 (in) : -713.7 Transmembrane helix 8 from 187 (in) to 202 (out) : -369.38 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 6 (in) to 21 (out) : -625.334 Transmembrane helix 2 from 49 (out) to 64 (in) : -116.212 Transmembrane helix 3 from 87 (in) to 102 (out) : -163.397 Transmembrane helix 4 from 106 (out) to 121 (in) : -393.265 Transmembrane helix 5 from 136 (in) to 151 (out) : -187.367 Transmembrane helix 6 from 155 (out) to 170 (in) : -285.144 Transmembrane helix 7 from 187 (in) to 202 (out) : -369.38 Re-entrant helix 1 from 174 (in) to 183 (in) : 700 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 49 (out) to 64 (in) : -116.212 Transmembrane helix 2 from 87 (in) to 102 (out) : -163.397 Transmembrane helix 3 from 106 (out) to 121 (in) : -393.265 Transmembrane helix 4 from 136 (in) to 151 (out) : -187.367 Transmembrane helix 5 from 155 (out) to 170 (in) : -285.144 Transmembrane helix 6 from 187 (in) to 202 (out) : -369.38 Re-entrant helix 1 from 174 (in) to 183 (in) : 700 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 50 (in) to 65 (out) : -102.87 Transmembrane helix 2 from 91 (out) to 106 (in) : -121.774 Transmembrane helix 3 from 136 (in) to 151 (out) : -187.367 Transmembrane helix 4 from 155 (out) to 170 (in) : -285.144 Transmembrane helix 5 from 187 (in) to 202 (out) : -369.38 Re-entrant helix 1 from 174 (in) to 183 (in) : 700 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 49 (out) to 64 (in) : -116.212 Transmembrane helix 2 from 87 (in) to 102 (out) : -163.397 Transmembrane helix 3 from 106 (out) to 121 (in) : -393.265 Transmembrane helix 4 from 145 (in) to 160 (out) : 2.403 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 50 (in) to 65 (out) : -102.87 Transmembrane helix 2 from 91 (out) to 106 (in) : -121.774 Transmembrane helix 3 from 145 (in) to 160 (out) : 2.403 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 91 (out) to 106 (in) : -121.774 Transmembrane helix 2 from 145 (in) to 160 (out) : 2.403 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.002264 1 helix (-) : Score = 0.000385998 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0.299 Topology: 144-159 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.000385998