BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (45 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q0THU0 Stationary-phase-induced ribosome-associated pro...    93   3e-18
UniRef50_Q57P89 Stationary-phase-induced ribosome-associated pro...    82   7e-15

>UniRef50_Q0THU0 Stationary-phase-induced ribosome-associated protein n=47
          Tax=Enterobacteriaceae RepID=SRA_ECOL5
          Length = 45

 Score = 92.8 bits (229), Expect = 3e-18,   Method: Compositional matrix adjust.
 Identities = 45/45 (100%), Positives = 45/45 (100%)

Query: 1  MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAEK 45
          MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAEK
Sbjct: 1  MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAEK 45


>UniRef50_Q57P89 Stationary-phase-induced ribosome-associated protein n=28
          Tax=Enterobacteriaceae RepID=SRA_SALCH
          Length = 47

 Score = 81.6 bits (200), Expect = 7e-15,   Method: Compositional matrix adjust.
 Identities = 38/44 (86%), Positives = 42/44 (95%)

Query: 1  MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE 44
          MKSNRQARHILGLD++ISNQRK+VTEGD SSVVNNPTGRKR A+
Sbjct: 1  MKSNRQARHILGLDYRISNQRKVVTEGDTSSVVNNPTGRKRRAD 44


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_Q0THU0 Stationary-phase-induced ribosome-associated pro...    68   7e-11
UniRef50_Q57P89 Stationary-phase-induced ribosome-associated pro...    65   7e-10

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_Q0THU0 Stationary-phase-induced ribosome-associated protein n=47
          Tax=Enterobacteriaceae RepID=SRA_ECOL5
          Length = 45

 Score = 68.2 bits (165), Expect = 7e-11,   Method: Composition-based stats.
 Identities = 45/45 (100%), Positives = 45/45 (100%)

Query: 1  MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAEK 45
          MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAEK
Sbjct: 1  MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAEK 45


>UniRef50_Q57P89 Stationary-phase-induced ribosome-associated protein n=28
          Tax=Enterobacteriaceae RepID=SRA_SALCH
          Length = 47

 Score = 65.1 bits (157), Expect = 7e-10,   Method: Composition-based stats.
 Identities = 38/44 (86%), Positives = 42/44 (95%)

Query: 1  MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE 44
          MKSNRQARHILGLD++ISNQRK+VTEGD SSVVNNPTGRKR A+
Sbjct: 1  MKSNRQARHILGLDYRISNQRKVVTEGDTSSVVNNPTGRKRRAD 44


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.303    0.132    0.362 

Lambda     K      H
   0.267   0.0391    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 158,040,219
Number of Sequences: 3077464
Number of extensions: 3462948
Number of successful extensions: 5857
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 5853
Number of HSP's gapped (non-prelim): 4
length of query: 45
length of database: 1,040,396,356
effective HSP length: 19
effective length of query: 26
effective length of database: 981,924,540
effective search space: 25530038040
effective search space used: 25530038040
T: 11
A: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.3 bits)
S2: 87 (38.2 bits)