Processing 1 helix: Transmembrane helix 1 from 115 (in) to 141 (out) : 4581.17 Score = 4.581165 Processing 1 helix: Transmembrane helix 1 from 115 (out) to 141 (in) : 4629.44 Score = 4.629435 Processing 22 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : -1377.17 Transmembrane helix 2 from 28 (out) to 43 (in) : -757.195 Transmembrane helix 3 from 47 (in) to 62 (out) : -971.343 Transmembrane helix 4 from 66 (out) to 81 (in) : 1870.45 Transmembrane helix 5 from 85 (in) to 100 (out) : 221.755 Transmembrane helix 6 from 104 (out) to 119 (in) : 524.34 Transmembrane helix 7 from 123 (in) to 138 (out) : 3404.03 Transmembrane helix 8 from 142 (out) to 157 (in) : -513.052 Transmembrane helix 9 from 161 (in) to 176 (out) : 1172.82 Transmembrane helix 10 from 180 (out) to 195 (in) : 930.813 Transmembrane helix 11 from 199 (in) to 214 (out) : 941.517 Transmembrane helix 12 from 218 (out) to 233 (in) : -1245.35 Transmembrane helix 13 from 237 (in) to 252 (out) : -1037 Transmembrane helix 14 from 256 (out) to 271 (in) : 513.491 Transmembrane helix 15 from 275 (in) to 290 (out) : 1752.95 Transmembrane helix 16 from 294 (out) to 309 (in) : -463.358 Transmembrane helix 17 from 313 (in) to 328 (out) : 2705.23 Transmembrane helix 18 from 332 (out) to 347 (in) : 450.943 Transmembrane helix 19 from 351 (in) to 366 (out) : -122.114 Transmembrane helix 20 from 370 (out) to 385 (in) : 715.105 Transmembrane helix 21 from 389 (in) to 404 (out) : 1939.52 Transmembrane helix 22 from 408 (out) to 423 (in) : -646.545 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 13 (out) to 28 (in) : -1212.04 Transmembrane helix 2 from 32 (in) to 47 (out) : -717.313 Transmembrane helix 3 from 51 (out) to 66 (in) : -931.402 Transmembrane helix 4 from 70 (in) to 85 (out) : 2436.58 Transmembrane helix 5 from 89 (out) to 104 (in) : -233.561 Transmembrane helix 6 from 108 (in) to 123 (out) : 1252.17 Transmembrane helix 7 from 127 (out) to 142 (in) : 2991.22 Transmembrane helix 8 from 146 (in) to 161 (out) : -454.201 Transmembrane helix 9 from 165 (out) to 180 (in) : 1068.23 Transmembrane helix 10 from 184 (in) to 199 (out) : 1626.33 Transmembrane helix 11 from 203 (out) to 218 (in) : 33.187 Transmembrane helix 12 from 232 (in) to 247 (out) : -960.61 Transmembrane helix 13 from 252 (out) to 267 (in) : -394.67 Transmembrane helix 14 from 271 (in) to 286 (out) : 2549.13 Transmembrane helix 15 from 290 (out) to 305 (in) : -460.255 Transmembrane helix 16 from 309 (in) to 324 (out) : 1898.82 Transmembrane helix 17 from 328 (out) to 343 (in) : 1484.87 Transmembrane helix 18 from 347 (in) to 362 (out) : -550.566 Transmembrane helix 19 from 366 (out) to 381 (in) : 1038.77 Transmembrane helix 20 from 385 (in) to 400 (out) : 1463.43 Transmembrane helix 21 from 404 (out) to 419 (in) : 47.539 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 26 (in) to 41 (out) : -770.916 Transmembrane helix 2 from 45 (out) to 60 (in) : -923.816 Transmembrane helix 3 from 69 (in) to 85 (out) : 2478.47 Transmembrane helix 4 from 89 (out) to 104 (in) : -233.561 Transmembrane helix 5 from 108 (in) to 123 (out) : 1252.17 Transmembrane helix 6 from 127 (out) to 142 (in) : 2991.22 Transmembrane helix 7 from 146 (in) to 161 (out) : -454.201 Transmembrane helix 8 from 165 (out) to 180 (in) : 1068.23 Transmembrane helix 9 from 184 (in) to 199 (out) : 1626.33 Transmembrane helix 10 from 203 (out) to 218 (in) : 33.187 Transmembrane helix 11 from 232 (in) to 247 (out) : -960.61 Transmembrane helix 12 from 252 (out) to 267 (in) : -394.67 Transmembrane helix 13 from 271 (in) to 286 (out) : 2549.13 Transmembrane helix 14 from 290 (out) to 305 (in) : -460.255 Transmembrane helix 15 from 309 (in) to 324 (out) : 1898.82 Transmembrane helix 16 from 328 (out) to 343 (in) : 1484.87 Transmembrane helix 17 from 347 (in) to 362 (out) : -550.566 Transmembrane helix 18 from 366 (out) to 381 (in) : 1038.77 Transmembrane helix 19 from 385 (in) to 400 (out) : 1463.43 Transmembrane helix 20 from 404 (out) to 419 (in) : 47.539 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 32 (out) to 47 (in) : -724.587 Transmembrane helix 2 from 51 (in) to 66 (out) : -944.198 Transmembrane helix 3 from 70 (out) to 85 (in) : 2413.82 Transmembrane helix 4 from 89 (in) to 104 (out) : -234.939 Transmembrane helix 5 from 108 (out) to 123 (in) : 1262.13 Transmembrane helix 6 from 127 (in) to 142 (out) : 2972.08 Transmembrane helix 7 from 146 (out) to 161 (in) : -447.299 Transmembrane helix 8 from 165 (in) to 180 (out) : 1090.47 Transmembrane helix 9 from 184 (out) to 199 (in) : 1646.47 Transmembrane helix 10 from 203 (in) to 218 (out) : 28.813 Transmembrane helix 11 from 232 (out) to 247 (in) : -959.69 Transmembrane helix 12 from 265 (in) to 282 (out) : 2943.94 Transmembrane helix 13 from 286 (out) to 301 (in) : -368.467 Transmembrane helix 14 from 305 (in) to 320 (out) : 1167.39 Transmembrane helix 15 from 324 (out) to 339 (in) : 2364.22 Transmembrane helix 16 from 344 (in) to 359 (out) : -708.668 Transmembrane helix 17 from 363 (out) to 378 (in) : 1083.14 Transmembrane helix 18 from 382 (in) to 397 (out) : 1023.38 Transmembrane helix 19 from 401 (out) to 416 (in) : 629.535 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 33 (in) to 48 (out) : -714.391 Transmembrane helix 2 from 69 (out) to 85 (in) : 2455.73 Transmembrane helix 3 from 89 (in) to 104 (out) : -234.939 Transmembrane helix 4 from 108 (out) to 123 (in) : 1262.13 Transmembrane helix 5 from 127 (in) to 142 (out) : 2972.08 Transmembrane helix 6 from 146 (out) to 161 (in) : -447.299 Transmembrane helix 7 from 165 (in) to 180 (out) : 1090.47 Transmembrane helix 8 from 184 (out) to 199 (in) : 1646.47 Transmembrane helix 9 from 203 (in) to 218 (out) : 28.813 Transmembrane helix 10 from 232 (out) to 247 (in) : -959.69 Transmembrane helix 11 from 265 (in) to 282 (out) : 2943.94 Transmembrane helix 12 from 286 (out) to 301 (in) : -368.467 Transmembrane helix 13 from 305 (in) to 320 (out) : 1167.39 Transmembrane helix 14 from 324 (out) to 339 (in) : 2364.22 Transmembrane helix 15 from 344 (in) to 359 (out) : -708.668 Transmembrane helix 16 from 363 (out) to 378 (in) : 1083.14 Transmembrane helix 17 from 382 (in) to 397 (out) : 1023.38 Transmembrane helix 18 from 401 (out) to 416 (in) : 629.535 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 35 (out) to 50 (in) : -722.134 Transmembrane helix 2 from 69 (in) to 85 (out) : 2478.47 Transmembrane helix 3 from 89 (out) to 104 (in) : -233.561 Transmembrane helix 4 from 108 (in) to 123 (out) : 1252.17 Transmembrane helix 5 from 127 (out) to 142 (in) : 2991.22 Transmembrane helix 6 from 146 (in) to 161 (out) : -454.201 Transmembrane helix 7 from 165 (out) to 180 (in) : 1068.23 Transmembrane helix 8 from 184 (in) to 199 (out) : 1626.33 Transmembrane helix 9 from 203 (out) to 218 (in) : 33.187 Transmembrane helix 10 from 265 (in) to 282 (out) : 2943.94 Transmembrane helix 11 from 286 (out) to 301 (in) : -368.467 Transmembrane helix 12 from 305 (in) to 320 (out) : 1167.39 Transmembrane helix 13 from 324 (out) to 339 (in) : 2364.22 Transmembrane helix 14 from 344 (in) to 359 (out) : -708.668 Transmembrane helix 15 from 363 (out) to 378 (in) : 1083.14 Transmembrane helix 16 from 382 (in) to 397 (out) : 1023.38 Transmembrane helix 17 from 401 (out) to 416 (in) : 629.535 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 33 (in) to 48 (out) : -714.391 Transmembrane helix 2 from 69 (out) to 85 (in) : 2455.73 Transmembrane helix 3 from 89 (in) to 104 (out) : -234.939 Transmembrane helix 4 from 108 (out) to 123 (in) : 1262.13 Transmembrane helix 5 from 127 (in) to 142 (out) : 2972.08 Transmembrane helix 6 from 152 (out) to 167 (in) : 276.353 Transmembrane helix 7 from 171 (in) to 186 (out) : 556.838 Transmembrane helix 8 from 190 (out) to 208 (in) : 2424.55 Transmembrane helix 9 from 265 (in) to 282 (out) : 2943.94 Transmembrane helix 10 from 286 (out) to 301 (in) : -368.467 Transmembrane helix 11 from 305 (in) to 320 (out) : 1167.39 Transmembrane helix 12 from 324 (out) to 339 (in) : 2364.22 Transmembrane helix 13 from 344 (in) to 359 (out) : -708.668 Transmembrane helix 14 from 363 (out) to 378 (in) : 1083.14 Transmembrane helix 15 from 382 (in) to 397 (out) : 1023.38 Transmembrane helix 16 from 401 (out) to 416 (in) : 629.535 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 35 (out) to 50 (in) : -722.134 Transmembrane helix 2 from 69 (in) to 85 (out) : 2478.47 Transmembrane helix 3 from 89 (out) to 104 (in) : -233.561 Transmembrane helix 4 from 108 (in) to 123 (out) : 1252.17 Transmembrane helix 5 from 127 (out) to 142 (in) : 2991.22 Transmembrane helix 6 from 153 (in) to 168 (out) : 401.595 Transmembrane helix 7 from 172 (out) to 187 (in) : 445.718 Transmembrane helix 8 from 191 (in) to 208 (out) : 2368.26 Transmembrane helix 9 from 265 (out) to 285 (in) : 3110.02 Transmembrane helix 10 from 289 (in) to 304 (out) : -457.462 Transmembrane helix 11 from 308 (out) to 323 (in) : 1723.51 Transmembrane helix 12 from 327 (in) to 342 (out) : 1726.08 Transmembrane helix 13 from 354 (out) to 369 (in) : 271.527 Transmembrane helix 14 from 373 (in) to 388 (out) : 495.184 Transmembrane helix 15 from 392 (out) to 409 (in) : 2080.86 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 69 (in) to 85 (out) : 2478.47 Transmembrane helix 2 from 89 (out) to 104 (in) : -233.561 Transmembrane helix 3 from 108 (in) to 123 (out) : 1252.17 Transmembrane helix 4 from 127 (out) to 142 (in) : 2991.22 Transmembrane helix 5 from 153 (in) to 168 (out) : 401.595 Transmembrane helix 6 from 172 (out) to 187 (in) : 445.718 Transmembrane helix 7 from 191 (in) to 208 (out) : 2368.26 Transmembrane helix 8 from 265 (out) to 285 (in) : 3110.02 Transmembrane helix 9 from 289 (in) to 304 (out) : -457.462 Transmembrane helix 10 from 308 (out) to 323 (in) : 1723.51 Transmembrane helix 11 from 327 (in) to 342 (out) : 1726.08 Transmembrane helix 12 from 354 (out) to 369 (in) : 271.527 Transmembrane helix 13 from 373 (in) to 388 (out) : 495.184 Transmembrane helix 14 from 392 (out) to 409 (in) : 2080.86 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 69 (out) to 85 (in) : 2455.73 Transmembrane helix 2 from 89 (in) to 104 (out) : -234.939 Transmembrane helix 3 from 108 (out) to 123 (in) : 1262.13 Transmembrane helix 4 from 127 (in) to 142 (out) : 2972.08 Transmembrane helix 5 from 152 (out) to 167 (in) : 276.353 Transmembrane helix 6 from 171 (in) to 186 (out) : 556.838 Transmembrane helix 7 from 190 (out) to 208 (in) : 2424.55 Transmembrane helix 8 from 265 (in) to 286 (out) : 3159.03 Transmembrane helix 9 from 305 (out) to 320 (in) : 1190.41 Transmembrane helix 10 from 324 (in) to 339 (out) : 2352.38 Transmembrane helix 11 from 354 (out) to 369 (in) : 271.527 Transmembrane helix 12 from 373 (in) to 388 (out) : 495.184 Transmembrane helix 13 from 392 (out) to 409 (in) : 2080.86 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 69 (in) to 85 (out) : 2478.47 Transmembrane helix 2 from 89 (out) to 104 (in) : -233.561 Transmembrane helix 3 from 108 (in) to 123 (out) : 1252.17 Transmembrane helix 4 from 127 (out) to 142 (in) : 2991.22 Transmembrane helix 5 from 161 (in) to 177 (out) : 1202.26 Transmembrane helix 6 from 187 (out) to 208 (in) : 2470.17 Transmembrane helix 7 from 265 (in) to 286 (out) : 3159.03 Transmembrane helix 8 from 305 (out) to 320 (in) : 1190.41 Transmembrane helix 9 from 324 (in) to 339 (out) : 2352.38 Transmembrane helix 10 from 354 (out) to 369 (in) : 271.527 Transmembrane helix 11 from 373 (in) to 388 (out) : 495.184 Transmembrane helix 12 from 392 (out) to 409 (in) : 2080.86 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 69 (out) to 85 (in) : 2455.73 Transmembrane helix 2 from 89 (in) to 104 (out) : -234.939 Transmembrane helix 3 from 108 (out) to 123 (in) : 1262.13 Transmembrane helix 4 from 127 (in) to 142 (out) : 2972.08 Transmembrane helix 5 from 152 (out) to 167 (in) : 276.353 Transmembrane helix 6 from 171 (in) to 186 (out) : 556.838 Transmembrane helix 7 from 190 (out) to 208 (in) : 2424.55 Transmembrane helix 8 from 265 (in) to 286 (out) : 3159.03 Transmembrane helix 9 from 312 (out) to 338 (in) : 4022.69 Transmembrane helix 10 from 364 (in) to 379 (out) : 1110.67 Transmembrane helix 11 from 387 (out) to 409 (in) : 2202.24 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 69 (in) to 85 (out) : 2478.47 Transmembrane helix 2 from 89 (out) to 104 (in) : -233.561 Transmembrane helix 3 from 108 (in) to 123 (out) : 1252.17 Transmembrane helix 4 from 127 (out) to 142 (in) : 2991.22 Transmembrane helix 5 from 161 (in) to 177 (out) : 1202.26 Transmembrane helix 6 from 187 (out) to 208 (in) : 2470.17 Transmembrane helix 7 from 265 (in) to 286 (out) : 3159.03 Transmembrane helix 8 from 312 (out) to 338 (in) : 4022.69 Transmembrane helix 9 from 364 (in) to 379 (out) : 1110.67 Transmembrane helix 10 from 387 (out) to 409 (in) : 2202.24 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 69 (out) to 89 (in) : 2635.98 Transmembrane helix 2 from 96 (in) to 111 (out) : -303.013 Transmembrane helix 3 from 115 (out) to 141 (in) : 4629.44 Transmembrane helix 4 from 161 (in) to 177 (out) : 1202.26 Transmembrane helix 5 from 187 (out) to 208 (in) : 2470.17 Transmembrane helix 6 from 265 (in) to 286 (out) : 3159.03 Transmembrane helix 7 from 312 (out) to 338 (in) : 4022.69 Transmembrane helix 8 from 364 (in) to 379 (out) : 1110.67 Transmembrane helix 9 from 387 (out) to 409 (in) : 2202.24 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 69 (in) to 89 (out) : 2675.02 Transmembrane helix 2 from 115 (out) to 141 (in) : 4629.44 Transmembrane helix 3 from 161 (in) to 177 (out) : 1202.26 Transmembrane helix 4 from 187 (out) to 208 (in) : 2470.17 Transmembrane helix 5 from 265 (in) to 286 (out) : 3159.03 Transmembrane helix 6 from 312 (out) to 338 (in) : 4022.69 Transmembrane helix 7 from 364 (in) to 379 (out) : 1110.67 Transmembrane helix 8 from 387 (out) to 409 (in) : 2202.24 Score = 21.663609 Processing 7 helices: Transmembrane helix 1 from 69 (out) to 89 (in) : 2635.98 Transmembrane helix 2 from 114 (in) to 141 (out) : 4578.87 Transmembrane helix 3 from 175 (out) to 205 (in) : 2556.97 Transmembrane helix 4 from 265 (in) to 286 (out) : 3159.03 Transmembrane helix 5 from 312 (out) to 338 (in) : 4022.69 Transmembrane helix 6 from 364 (in) to 379 (out) : 1110.67 Transmembrane helix 7 from 387 (out) to 409 (in) : 2202.24 Score = 20.275555 Processing 6 helices: Transmembrane helix 1 from 69 (in) to 89 (out) : 2675.02 Transmembrane helix 2 from 115 (out) to 141 (in) : 4629.44 Transmembrane helix 3 from 173 (in) to 203 (out) : 2554.51 Transmembrane helix 4 from 265 (out) to 286 (in) : 3124.77 Transmembrane helix 5 from 312 (in) to 338 (out) : 3989.51 Transmembrane helix 6 from 374 (out) to 404 (in) : 2319.33 Score = 19.300688 Processing 5 helices: Transmembrane helix 1 from 69 (out) to 89 (in) : 2635.98 Transmembrane helix 2 from 114 (in) to 141 (out) : 4578.87 Transmembrane helix 3 from 175 (out) to 205 (in) : 2556.97 Transmembrane helix 4 from 265 (in) to 286 (out) : 3159.03 Transmembrane helix 5 from 312 (out) to 338 (in) : 4022.69 Score = 16.901871 Processing 4 helices: Transmembrane helix 1 from 69 (in) to 89 (out) : 2675.02 Transmembrane helix 2 from 115 (out) to 141 (in) : 4629.44 Transmembrane helix 3 from 265 (in) to 286 (out) : 3159.03 Transmembrane helix 4 from 312 (out) to 338 (in) : 4022.69 Score = 14.566813 Processing 3 helices: Transmembrane helix 1 from 115 (out) to 141 (in) : 4629.44 Transmembrane helix 2 from 265 (in) to 286 (out) : 3159.03 Transmembrane helix 3 from 312 (out) to 338 (in) : 4022.69 Score = 11.850836 Processing 2 helices: Transmembrane helix 1 from 115 (in) to 141 (out) : 4581.17 Transmembrane helix 2 from 312 (out) to 338 (in) : 4022.69 Score = 8.603858 Processing 22 helices: Transmembrane helix 1 from 9 (out) to 24 (in) : -1382.33 Transmembrane helix 2 from 28 (in) to 43 (out) : -748.305 Transmembrane helix 3 from 47 (out) to 62 (in) : -950.057 Transmembrane helix 4 from 66 (in) to 81 (out) : 1876.35 Transmembrane helix 5 from 85 (out) to 100 (in) : 221.645 Transmembrane helix 6 from 104 (in) to 119 (out) : 535.16 Transmembrane helix 7 from 123 (out) to 138 (in) : 3427.17 Transmembrane helix 8 from 142 (in) to 157 (out) : -515.748 Transmembrane helix 9 from 161 (out) to 176 (in) : 1150.38 Transmembrane helix 10 from 180 (in) to 195 (out) : 932.687 Transmembrane helix 11 from 199 (out) to 214 (in) : 938.283 Transmembrane helix 12 from 218 (in) to 233 (out) : -1243.95 Transmembrane helix 13 from 237 (out) to 252 (in) : -1038.6 Transmembrane helix 14 from 256 (in) to 271 (out) : 513.009 Transmembrane helix 15 from 275 (out) to 290 (in) : 1738.45 Transmembrane helix 16 from 294 (in) to 309 (out) : -458.142 Transmembrane helix 17 from 313 (out) to 328 (in) : 2718.17 Transmembrane helix 18 from 332 (in) to 347 (out) : 456.757 Transmembrane helix 19 from 351 (out) to 366 (in) : -115.986 Transmembrane helix 20 from 370 (in) to 385 (out) : 741.495 Transmembrane helix 21 from 389 (out) to 404 (in) : 1959.88 Transmembrane helix 22 from 408 (in) to 423 (out) : -646.155 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -1220.16 Transmembrane helix 2 from 32 (out) to 47 (in) : -724.587 Transmembrane helix 3 from 51 (in) to 66 (out) : -944.198 Transmembrane helix 4 from 70 (out) to 85 (in) : 2413.82 Transmembrane helix 5 from 89 (in) to 104 (out) : -234.939 Transmembrane helix 6 from 108 (out) to 123 (in) : 1262.13 Transmembrane helix 7 from 127 (in) to 142 (out) : 2972.08 Transmembrane helix 8 from 146 (out) to 161 (in) : -447.299 Transmembrane helix 9 from 165 (in) to 180 (out) : 1090.47 Transmembrane helix 10 from 184 (out) to 199 (in) : 1646.47 Transmembrane helix 11 from 203 (in) to 218 (out) : 28.813 Transmembrane helix 12 from 232 (out) to 247 (in) : -959.69 Transmembrane helix 13 from 252 (in) to 267 (out) : -406.53 Transmembrane helix 14 from 271 (out) to 286 (in) : 2524.47 Transmembrane helix 15 from 290 (in) to 305 (out) : -465.745 Transmembrane helix 16 from 309 (out) to 324 (in) : 1919.88 Transmembrane helix 17 from 328 (in) to 343 (out) : 1480.43 Transmembrane helix 18 from 347 (out) to 362 (in) : -537.834 Transmembrane helix 19 from 366 (in) to 381 (out) : 1063.03 Transmembrane helix 20 from 385 (out) to 400 (in) : 1482.97 Transmembrane helix 21 from 404 (in) to 419 (out) : 37.361 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 32 (out) to 47 (in) : -724.587 Transmembrane helix 2 from 51 (in) to 66 (out) : -944.198 Transmembrane helix 3 from 70 (out) to 85 (in) : 2413.82 Transmembrane helix 4 from 89 (in) to 104 (out) : -234.939 Transmembrane helix 5 from 108 (out) to 123 (in) : 1262.13 Transmembrane helix 6 from 127 (in) to 142 (out) : 2972.08 Transmembrane helix 7 from 146 (out) to 161 (in) : -447.299 Transmembrane helix 8 from 165 (in) to 180 (out) : 1090.47 Transmembrane helix 9 from 184 (out) to 199 (in) : 1646.47 Transmembrane helix 10 from 203 (in) to 218 (out) : 28.813 Transmembrane helix 11 from 232 (out) to 247 (in) : -959.69 Transmembrane helix 12 from 252 (in) to 267 (out) : -406.53 Transmembrane helix 13 from 271 (out) to 286 (in) : 2524.47 Transmembrane helix 14 from 290 (in) to 305 (out) : -465.745 Transmembrane helix 15 from 309 (out) to 324 (in) : 1919.88 Transmembrane helix 16 from 328 (in) to 343 (out) : 1480.43 Transmembrane helix 17 from 347 (out) to 362 (in) : -537.834 Transmembrane helix 18 from 366 (in) to 381 (out) : 1063.03 Transmembrane helix 19 from 385 (out) to 400 (in) : 1482.97 Transmembrane helix 20 from 404 (in) to 419 (out) : 37.361 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 26 (in) to 41 (out) : -770.916 Transmembrane helix 2 from 45 (out) to 60 (in) : -923.816 Transmembrane helix 3 from 69 (in) to 85 (out) : 2478.47 Transmembrane helix 4 from 89 (out) to 104 (in) : -233.561 Transmembrane helix 5 from 108 (in) to 123 (out) : 1252.17 Transmembrane helix 6 from 127 (out) to 142 (in) : 2991.22 Transmembrane helix 7 from 146 (in) to 161 (out) : -454.201 Transmembrane helix 8 from 165 (out) to 180 (in) : 1068.23 Transmembrane helix 9 from 184 (in) to 199 (out) : 1626.33 Transmembrane helix 10 from 203 (out) to 218 (in) : 33.187 Transmembrane helix 11 from 232 (in) to 247 (out) : -960.61 Transmembrane helix 12 from 265 (out) to 284 (in) : 3062.75 Transmembrane helix 13 from 288 (in) to 303 (out) : -439.553 Transmembrane helix 14 from 307 (out) to 322 (in) : 1519.11 Transmembrane helix 15 from 326 (in) to 341 (out) : 1955.38 Transmembrane helix 16 from 345 (out) to 360 (in) : -665.512 Transmembrane helix 17 from 364 (in) to 379 (out) : 1110.67 Transmembrane helix 18 from 383 (out) to 398 (in) : 1187.33 Transmembrane helix 19 from 402 (in) to 417 (out) : 420.476 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 35 (out) to 50 (in) : -722.134 Transmembrane helix 2 from 69 (in) to 85 (out) : 2478.47 Transmembrane helix 3 from 89 (out) to 104 (in) : -233.561 Transmembrane helix 4 from 108 (in) to 123 (out) : 1252.17 Transmembrane helix 5 from 127 (out) to 142 (in) : 2991.22 Transmembrane helix 6 from 146 (in) to 161 (out) : -454.201 Transmembrane helix 7 from 165 (out) to 180 (in) : 1068.23 Transmembrane helix 8 from 184 (in) to 199 (out) : 1626.33 Transmembrane helix 9 from 203 (out) to 218 (in) : 33.187 Transmembrane helix 10 from 232 (in) to 247 (out) : -960.61 Transmembrane helix 11 from 265 (out) to 284 (in) : 3062.75 Transmembrane helix 12 from 288 (in) to 303 (out) : -439.553 Transmembrane helix 13 from 307 (out) to 322 (in) : 1519.11 Transmembrane helix 14 from 326 (in) to 341 (out) : 1955.38 Transmembrane helix 15 from 345 (out) to 360 (in) : -665.512 Transmembrane helix 16 from 364 (in) to 379 (out) : 1110.67 Transmembrane helix 17 from 383 (out) to 398 (in) : 1187.33 Transmembrane helix 18 from 402 (in) to 417 (out) : 420.476 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 33 (in) to 48 (out) : -714.391 Transmembrane helix 2 from 69 (out) to 85 (in) : 2455.73 Transmembrane helix 3 from 89 (in) to 104 (out) : -234.939 Transmembrane helix 4 from 108 (out) to 123 (in) : 1262.13 Transmembrane helix 5 from 127 (in) to 142 (out) : 2972.08 Transmembrane helix 6 from 146 (out) to 161 (in) : -447.299 Transmembrane helix 7 from 165 (in) to 180 (out) : 1090.47 Transmembrane helix 8 from 184 (out) to 199 (in) : 1646.47 Transmembrane helix 9 from 203 (in) to 218 (out) : 28.813 Transmembrane helix 10 from 265 (out) to 284 (in) : 3062.75 Transmembrane helix 11 from 288 (in) to 303 (out) : -439.553 Transmembrane helix 12 from 307 (out) to 322 (in) : 1519.11 Transmembrane helix 13 from 326 (in) to 341 (out) : 1955.38 Transmembrane helix 14 from 345 (out) to 360 (in) : -665.512 Transmembrane helix 15 from 364 (in) to 379 (out) : 1110.67 Transmembrane helix 16 from 383 (out) to 398 (in) : 1187.33 Transmembrane helix 17 from 402 (in) to 417 (out) : 420.476 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 35 (out) to 50 (in) : -722.134 Transmembrane helix 2 from 69 (in) to 85 (out) : 2478.47 Transmembrane helix 3 from 89 (out) to 104 (in) : -233.561 Transmembrane helix 4 from 108 (in) to 123 (out) : 1252.17 Transmembrane helix 5 from 127 (out) to 142 (in) : 2991.22 Transmembrane helix 6 from 153 (in) to 168 (out) : 401.595 Transmembrane helix 7 from 172 (out) to 187 (in) : 445.718 Transmembrane helix 8 from 191 (in) to 208 (out) : 2368.26 Transmembrane helix 9 from 265 (out) to 284 (in) : 3062.75 Transmembrane helix 10 from 288 (in) to 303 (out) : -439.553 Transmembrane helix 11 from 307 (out) to 322 (in) : 1519.11 Transmembrane helix 12 from 326 (in) to 341 (out) : 1955.38 Transmembrane helix 13 from 345 (out) to 360 (in) : -665.512 Transmembrane helix 14 from 364 (in) to 379 (out) : 1110.67 Transmembrane helix 15 from 383 (out) to 398 (in) : 1187.33 Transmembrane helix 16 from 402 (in) to 417 (out) : 420.476 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 33 (in) to 48 (out) : -714.391 Transmembrane helix 2 from 69 (out) to 85 (in) : 2455.73 Transmembrane helix 3 from 89 (in) to 104 (out) : -234.939 Transmembrane helix 4 from 108 (out) to 123 (in) : 1262.13 Transmembrane helix 5 from 127 (in) to 142 (out) : 2972.08 Transmembrane helix 6 from 152 (out) to 167 (in) : 276.353 Transmembrane helix 7 from 171 (in) to 186 (out) : 556.838 Transmembrane helix 8 from 190 (out) to 208 (in) : 2424.55 Transmembrane helix 9 from 265 (in) to 285 (out) : 3142.38 Transmembrane helix 10 from 289 (out) to 304 (in) : -452.038 Transmembrane helix 11 from 308 (in) to 323 (out) : 1701.79 Transmembrane helix 12 from 327 (out) to 342 (in) : 1733.42 Transmembrane helix 13 from 355 (in) to 370 (out) : 400.738 Transmembrane helix 14 from 374 (out) to 389 (in) : 412.886 Transmembrane helix 15 from 393 (in) to 408 (out) : 2005.5 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 69 (out) to 85 (in) : 2455.73 Transmembrane helix 2 from 89 (in) to 104 (out) : -234.939 Transmembrane helix 3 from 108 (out) to 123 (in) : 1262.13 Transmembrane helix 4 from 127 (in) to 142 (out) : 2972.08 Transmembrane helix 5 from 152 (out) to 167 (in) : 276.353 Transmembrane helix 6 from 171 (in) to 186 (out) : 556.838 Transmembrane helix 7 from 190 (out) to 208 (in) : 2424.55 Transmembrane helix 8 from 265 (in) to 285 (out) : 3142.38 Transmembrane helix 9 from 289 (out) to 304 (in) : -452.038 Transmembrane helix 10 from 308 (in) to 323 (out) : 1701.79 Transmembrane helix 11 from 327 (out) to 342 (in) : 1733.42 Transmembrane helix 12 from 355 (in) to 370 (out) : 400.738 Transmembrane helix 13 from 374 (out) to 389 (in) : 412.886 Transmembrane helix 14 from 393 (in) to 408 (out) : 2005.5 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 69 (in) to 85 (out) : 2478.47 Transmembrane helix 2 from 89 (out) to 104 (in) : -233.561 Transmembrane helix 3 from 108 (in) to 123 (out) : 1252.17 Transmembrane helix 4 from 127 (out) to 142 (in) : 2991.22 Transmembrane helix 5 from 153 (in) to 168 (out) : 401.595 Transmembrane helix 6 from 172 (out) to 187 (in) : 445.718 Transmembrane helix 7 from 191 (in) to 208 (out) : 2368.26 Transmembrane helix 8 from 265 (out) to 286 (in) : 3124.77 Transmembrane helix 9 from 290 (in) to 305 (out) : -465.745 Transmembrane helix 10 from 312 (out) to 338 (in) : 4022.69 Transmembrane helix 11 from 355 (in) to 370 (out) : 400.738 Transmembrane helix 12 from 374 (out) to 389 (in) : 412.886 Transmembrane helix 13 from 393 (in) to 408 (out) : 2005.5 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 69 (out) to 85 (in) : 2455.73 Transmembrane helix 2 from 89 (in) to 104 (out) : -234.939 Transmembrane helix 3 from 108 (out) to 123 (in) : 1262.13 Transmembrane helix 4 from 127 (in) to 142 (out) : 2972.08 Transmembrane helix 5 from 152 (out) to 167 (in) : 276.353 Transmembrane helix 6 from 171 (in) to 186 (out) : 556.838 Transmembrane helix 7 from 190 (out) to 208 (in) : 2424.55 Transmembrane helix 8 from 265 (in) to 286 (out) : 3159.03 Transmembrane helix 9 from 312 (out) to 338 (in) : 4022.69 Transmembrane helix 10 from 355 (in) to 370 (out) : 400.738 Transmembrane helix 11 from 374 (out) to 389 (in) : 412.886 Transmembrane helix 12 from 393 (in) to 408 (out) : 2005.5 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 69 (in) to 85 (out) : 2478.47 Transmembrane helix 2 from 89 (out) to 104 (in) : -233.561 Transmembrane helix 3 from 108 (in) to 123 (out) : 1252.17 Transmembrane helix 4 from 127 (out) to 142 (in) : 2991.22 Transmembrane helix 5 from 161 (in) to 177 (out) : 1202.26 Transmembrane helix 6 from 187 (out) to 208 (in) : 2470.17 Transmembrane helix 7 from 265 (in) to 286 (out) : 3159.03 Transmembrane helix 8 from 312 (out) to 338 (in) : 4022.69 Transmembrane helix 9 from 355 (in) to 370 (out) : 400.738 Transmembrane helix 10 from 374 (out) to 389 (in) : 412.886 Transmembrane helix 11 from 393 (in) to 408 (out) : 2005.5 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 69 (out) to 89 (in) : 2635.98 Transmembrane helix 2 from 96 (in) to 111 (out) : -303.013 Transmembrane helix 3 from 115 (out) to 141 (in) : 4629.44 Transmembrane helix 4 from 161 (in) to 177 (out) : 1202.26 Transmembrane helix 5 from 187 (out) to 208 (in) : 2470.17 Transmembrane helix 6 from 265 (in) to 286 (out) : 3159.03 Transmembrane helix 7 from 312 (out) to 338 (in) : 4022.69 Transmembrane helix 8 from 355 (in) to 370 (out) : 400.738 Transmembrane helix 9 from 374 (out) to 389 (in) : 412.886 Transmembrane helix 10 from 393 (in) to 408 (out) : 2005.5 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 69 (in) to 89 (out) : 2675.02 Transmembrane helix 2 from 115 (out) to 141 (in) : 4629.44 Transmembrane helix 3 from 161 (in) to 177 (out) : 1202.26 Transmembrane helix 4 from 187 (out) to 208 (in) : 2470.17 Transmembrane helix 5 from 265 (in) to 286 (out) : 3159.03 Transmembrane helix 6 from 312 (out) to 338 (in) : 4022.69 Transmembrane helix 7 from 355 (in) to 370 (out) : 400.738 Transmembrane helix 8 from 374 (out) to 389 (in) : 412.886 Transmembrane helix 9 from 393 (in) to 408 (out) : 2005.5 Score = 21.111023 Processing 8 helices: Transmembrane helix 1 from 69 (out) to 89 (in) : 2635.98 Transmembrane helix 2 from 114 (in) to 141 (out) : 4578.87 Transmembrane helix 3 from 161 (out) to 177 (in) : 1177.14 Transmembrane helix 4 from 186 (in) to 208 (out) : 2440.44 Transmembrane helix 5 from 265 (out) to 286 (in) : 3124.77 Transmembrane helix 6 from 312 (in) to 338 (out) : 3989.51 Transmembrane helix 7 from 364 (out) to 379 (in) : 1086.83 Transmembrane helix 8 from 387 (in) to 408 (out) : 2172.46 Score = 21.017645 Processing 7 helices: Transmembrane helix 1 from 69 (in) to 89 (out) : 2675.02 Transmembrane helix 2 from 115 (out) to 141 (in) : 4629.44 Transmembrane helix 3 from 161 (in) to 177 (out) : 1202.26 Transmembrane helix 4 from 187 (out) to 208 (in) : 2470.17 Transmembrane helix 5 from 265 (in) to 286 (out) : 3159.03 Transmembrane helix 6 from 312 (out) to 338 (in) : 4022.69 Transmembrane helix 7 from 374 (in) to 404 (out) : 2320.07 Score = 20.658546 Processing 6 helices: Transmembrane helix 1 from 69 (out) to 89 (in) : 2635.98 Transmembrane helix 2 from 114 (in) to 141 (out) : 4578.87 Transmembrane helix 3 from 175 (out) to 205 (in) : 2556.97 Transmembrane helix 4 from 265 (in) to 286 (out) : 3159.03 Transmembrane helix 5 from 312 (out) to 338 (in) : 4022.69 Transmembrane helix 6 from 374 (in) to 404 (out) : 2320.07 Score = 19.270493 Processing 5 helices: Transmembrane helix 1 from 69 (in) to 89 (out) : 2675.02 Transmembrane helix 2 from 115 (out) to 141 (in) : 4629.44 Transmembrane helix 3 from 173 (in) to 203 (out) : 2554.51 Transmembrane helix 4 from 265 (out) to 286 (in) : 3124.77 Transmembrane helix 5 from 312 (in) to 338 (out) : 3989.51 Score = 17.029909 Processing 4 helices: Transmembrane helix 1 from 115 (out) to 141 (in) : 4629.44 Transmembrane helix 2 from 173 (in) to 203 (out) : 2554.51 Transmembrane helix 3 from 265 (out) to 286 (in) : 3124.77 Transmembrane helix 4 from 312 (in) to 338 (out) : 3989.51 Score = 14.313932 Processing 3 helices: Transmembrane helix 1 from 115 (in) to 141 (out) : 4581.17 Transmembrane helix 2 from 265 (out) to 286 (in) : 3124.77 Transmembrane helix 3 from 312 (in) to 338 (out) : 3989.51 Score = 11.655766 Processing 2 helices: Transmembrane helix 1 from 115 (out) to 141 (in) : 4629.44 Transmembrane helix 2 from 312 (in) to 338 (out) : 3989.51 Score = 8.618941 Summary of topology analysis: 1 helix (+) : Score = 4.58117 1 helix (-) : Score = 4.62944 2 helices (+) : Score = 8.60386 2 helices (-) : Score = 8.61894 3 helices (+) : Score = 11.6558 3 helices (-) : Score = 11.8508 4 helices (+) : Score = 14.5668 4 helices (-) : Score = 14.3139 5 helices (+) : Score = 17.0299 5 helices (-) : Score = 16.9019 6 helices (+) : Score = 19.3007 6 helices (-) : Score = 19.2705 7 helices (+) : Score = 20.6585 7 helices (-) : Score = 20.2756 8 helices (+) : Score = 21.6636 8 helices (-) : Score = 21.0176 9 helices (+) : Score = 21.111 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 22 helices (+) : Score = -100000 22 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 69-89,115-141,161-177,187-208,265-286,312-338,364-379,387-409 Re-entrant helices: Not detected. Helix count: 8 N-terminal: in Score: 21.6636