Processing 1 helix: Transmembrane helix 1 from 16 (in) to 31 (out) : 588.723 Score = 0.549584 Processing 1 helix: Transmembrane helix 1 from 16 (out) to 31 (in) : 571.077 Score = 0.610216 Processing 13 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -14070.5 Transmembrane helix 2 from 23 (out) to 38 (in) : -490.944 Transmembrane helix 3 from 42 (in) to 57 (out) : -886.299 Transmembrane helix 4 from 61 (out) to 76 (in) : -143.166 Transmembrane helix 5 from 80 (in) to 95 (out) : 0.757 Transmembrane helix 6 from 99 (out) to 114 (in) : 5.005 Transmembrane helix 7 from 118 (in) to 133 (out) : 2.086 Transmembrane helix 8 from 137 (out) to 152 (in) : 2.194 Transmembrane helix 9 from 156 (in) to 171 (out) : 0 Transmembrane helix 10 from 175 (out) to 190 (in) : 0 Transmembrane helix 11 from 194 (in) to 209 (out) : 0 Transmembrane helix 12 from 213 (out) to 228 (in) : 0 Transmembrane helix 13 from 232 (in) to 247 (out) : 0 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 571.077 Transmembrane helix 2 from 42 (in) to 57 (out) : -886.299 Transmembrane helix 3 from 61 (out) to 76 (in) : -143.166 Transmembrane helix 4 from 80 (in) to 95 (out) : 0.757 Transmembrane helix 5 from 99 (out) to 114 (in) : 5.005 Transmembrane helix 6 from 118 (in) to 133 (out) : 2.086 Transmembrane helix 7 from 137 (out) to 152 (in) : 2.194 Transmembrane helix 8 from 156 (in) to 171 (out) : 0 Transmembrane helix 9 from 175 (out) to 190 (in) : 0 Transmembrane helix 10 from 194 (in) to 209 (out) : 0 Transmembrane helix 11 from 213 (out) to 228 (in) : 0 Transmembrane helix 12 from 232 (in) to 247 (out) : 0 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 588.723 Transmembrane helix 2 from 61 (out) to 76 (in) : -143.166 Transmembrane helix 3 from 80 (in) to 95 (out) : 0.757 Transmembrane helix 4 from 99 (out) to 114 (in) : 5.005 Transmembrane helix 5 from 118 (in) to 133 (out) : 2.086 Transmembrane helix 6 from 137 (out) to 152 (in) : 2.194 Transmembrane helix 7 from 156 (in) to 171 (out) : 0 Transmembrane helix 8 from 175 (out) to 190 (in) : 0 Transmembrane helix 9 from 194 (in) to 209 (out) : 0 Transmembrane helix 10 from 213 (out) to 228 (in) : 0 Transmembrane helix 11 from 232 (in) to 247 (out) : 0 Re-entrant helix 1 from 35 (out) to 57 (out) : 1610 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 571.077 Transmembrane helix 2 from 63 (in) to 78 (out) : 7.576 Transmembrane helix 3 from 99 (out) to 114 (in) : 5.005 Transmembrane helix 4 from 118 (in) to 133 (out) : 2.086 Transmembrane helix 5 from 137 (out) to 152 (in) : 2.194 Transmembrane helix 6 from 156 (in) to 171 (out) : 0 Transmembrane helix 7 from 175 (out) to 190 (in) : 0 Transmembrane helix 8 from 194 (in) to 209 (out) : 0 Transmembrane helix 9 from 213 (out) to 228 (in) : 0 Transmembrane helix 10 from 232 (in) to 247 (out) : 0 Re-entrant helix 1 from 35 (in) to 59 (in) : 1750 Re-entrant helix 2 from 82 (out) to 95 (out) : 980 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 588.723 Transmembrane helix 2 from 92 (out) to 109 (in) : 5.874 Transmembrane helix 3 from 113 (in) to 128 (out) : 1.963 Transmembrane helix 4 from 132 (out) to 147 (in) : 5.98 Transmembrane helix 5 from 151 (in) to 166 (out) : 0.352 Transmembrane helix 6 from 170 (out) to 185 (in) : 0 Transmembrane helix 7 from 189 (in) to 204 (out) : 0 Transmembrane helix 8 from 208 (out) to 223 (in) : 0 Transmembrane helix 9 from 227 (in) to 242 (out) : 0 Re-entrant helix 1 from 35 (out) to 69 (out) : 2450 Re-entrant helix 2 from 70 (out) to 88 (out) : 1330 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 571.077 Transmembrane helix 2 from 63 (in) to 78 (out) : 7.576 Transmembrane helix 3 from 132 (out) to 147 (in) : 5.98 Transmembrane helix 4 from 151 (in) to 166 (out) : 0.352 Transmembrane helix 5 from 170 (out) to 185 (in) : 0 Transmembrane helix 6 from 189 (in) to 204 (out) : 0 Transmembrane helix 7 from 208 (out) to 223 (in) : 0 Transmembrane helix 8 from 227 (in) to 242 (out) : 0 Re-entrant helix 1 from 35 (in) to 59 (in) : 1750 Re-entrant helix 2 from 82 (out) to 116 (out) : 2450 Re-entrant helix 3 from 117 (out) to 128 (out) : 840 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 588.723 Transmembrane helix 2 from 92 (out) to 109 (in) : 5.874 Transmembrane helix 3 from 113 (in) to 128 (out) : 1.963 Transmembrane helix 4 from 132 (out) to 147 (in) : 5.98 Transmembrane helix 5 from 151 (in) to 166 (out) : 0.352 Transmembrane helix 6 from 170 (out) to 185 (in) : 0 Transmembrane helix 7 from 189 (in) to 204 (out) : 0 Re-entrant helix 1 from 35 (out) to 69 (out) : 2450 Re-entrant helix 2 from 70 (out) to 88 (out) : 1330 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 571.077 Transmembrane helix 2 from 63 (in) to 78 (out) : 7.576 Transmembrane helix 3 from 132 (out) to 147 (in) : 5.98 Transmembrane helix 4 from 151 (in) to 166 (out) : 0.352 Transmembrane helix 5 from 170 (out) to 185 (in) : 0 Transmembrane helix 6 from 189 (in) to 204 (out) : 0 Re-entrant helix 1 from 35 (in) to 59 (in) : 1750 Re-entrant helix 2 from 82 (out) to 116 (out) : 2450 Re-entrant helix 3 from 117 (out) to 128 (out) : 840 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 588.723 Transmembrane helix 2 from 92 (out) to 109 (in) : 5.874 Transmembrane helix 3 from 113 (in) to 128 (out) : 1.963 Transmembrane helix 4 from 132 (out) to 147 (in) : 5.98 Transmembrane helix 5 from 151 (in) to 166 (out) : 0.352 Re-entrant helix 1 from 35 (out) to 69 (out) : 2450 Re-entrant helix 2 from 70 (out) to 88 (out) : 1330 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 571.077 Transmembrane helix 2 from 63 (in) to 78 (out) : 7.576 Transmembrane helix 3 from 132 (out) to 147 (in) : 5.98 Transmembrane helix 4 from 151 (in) to 166 (out) : 0.352 Re-entrant helix 1 from 35 (in) to 59 (in) : 1750 Re-entrant helix 2 from 82 (out) to 116 (out) : 2450 Re-entrant helix 3 from 117 (out) to 128 (out) : 840 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 588.723 Transmembrane helix 2 from 92 (out) to 122 (in) : 13.105 Transmembrane helix 3 from 184 (in) to 199 (out) : 0 Re-entrant helix 1 from 35 (out) to 69 (out) : 2450 Re-entrant helix 2 from 70 (out) to 88 (out) : 1330 Re-entrant helix 3 from 126 (in) to 160 (in) : 2450 Re-entrant helix 4 from 161 (in) to 180 (in) : 1400 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 571.077 Transmembrane helix 2 from 115 (in) to 133 (out) : 2.729 Re-entrant helix 1 from 35 (in) to 69 (in) : 2450 Re-entrant helix 2 from 70 (in) to 104 (in) : 2450 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -14038.7 Transmembrane helix 2 from 23 (in) to 38 (out) : -493.056 Transmembrane helix 3 from 42 (out) to 57 (in) : -871.401 Transmembrane helix 4 from 61 (in) to 76 (out) : -131.434 Transmembrane helix 5 from 80 (out) to 95 (in) : -0.757 Transmembrane helix 6 from 99 (in) to 114 (out) : -5.005 Transmembrane helix 7 from 118 (out) to 133 (in) : -2.086 Transmembrane helix 8 from 137 (in) to 152 (out) : -2.194 Transmembrane helix 9 from 156 (out) to 171 (in) : 0 Transmembrane helix 10 from 175 (in) to 190 (out) : 0 Transmembrane helix 11 from 194 (out) to 209 (in) : 0 Transmembrane helix 12 from 213 (in) to 228 (out) : 0 Transmembrane helix 13 from 232 (out) to 247 (in) : 0 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 588.723 Transmembrane helix 2 from 42 (out) to 57 (in) : -871.401 Transmembrane helix 3 from 61 (in) to 76 (out) : -131.434 Transmembrane helix 4 from 80 (out) to 95 (in) : -0.757 Transmembrane helix 5 from 99 (in) to 114 (out) : -5.005 Transmembrane helix 6 from 118 (out) to 133 (in) : -2.086 Transmembrane helix 7 from 137 (in) to 152 (out) : -2.194 Transmembrane helix 8 from 156 (out) to 171 (in) : 0 Transmembrane helix 9 from 175 (in) to 190 (out) : 0 Transmembrane helix 10 from 194 (out) to 209 (in) : 0 Transmembrane helix 11 from 213 (in) to 228 (out) : 0 Transmembrane helix 12 from 232 (out) to 247 (in) : 0 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 571.077 Transmembrane helix 2 from 61 (in) to 76 (out) : -131.434 Transmembrane helix 3 from 80 (out) to 95 (in) : -0.757 Transmembrane helix 4 from 99 (in) to 114 (out) : -5.005 Transmembrane helix 5 from 118 (out) to 133 (in) : -2.086 Transmembrane helix 6 from 137 (in) to 152 (out) : -2.194 Transmembrane helix 7 from 156 (out) to 171 (in) : 0 Transmembrane helix 8 from 175 (in) to 190 (out) : 0 Transmembrane helix 9 from 194 (out) to 209 (in) : 0 Transmembrane helix 10 from 213 (in) to 228 (out) : 0 Transmembrane helix 11 from 232 (out) to 247 (in) : 0 Re-entrant helix 1 from 35 (in) to 57 (in) : 1610 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 588.723 Transmembrane helix 2 from 80 (out) to 95 (in) : -0.757 Transmembrane helix 3 from 99 (in) to 114 (out) : -5.005 Transmembrane helix 4 from 118 (out) to 133 (in) : -2.086 Transmembrane helix 5 from 137 (in) to 152 (out) : -2.194 Transmembrane helix 6 from 156 (out) to 171 (in) : 0 Transmembrane helix 7 from 175 (in) to 190 (out) : 0 Transmembrane helix 8 from 194 (out) to 209 (in) : 0 Transmembrane helix 9 from 213 (in) to 228 (out) : 0 Transmembrane helix 10 from 232 (out) to 247 (in) : 0 Re-entrant helix 1 from 35 (out) to 69 (out) : 2450 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 571.077 Transmembrane helix 2 from 63 (in) to 78 (out) : 7.576 Transmembrane helix 3 from 118 (out) to 133 (in) : -2.086 Transmembrane helix 4 from 137 (in) to 152 (out) : -2.194 Transmembrane helix 5 from 156 (out) to 171 (in) : 0 Transmembrane helix 6 from 175 (in) to 190 (out) : 0 Transmembrane helix 7 from 194 (out) to 209 (in) : 0 Transmembrane helix 8 from 213 (in) to 228 (out) : 0 Transmembrane helix 9 from 232 (out) to 247 (in) : 0 Re-entrant helix 1 from 35 (in) to 59 (in) : 1750 Re-entrant helix 2 from 82 (out) to 114 (out) : 2310 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 588.723 Transmembrane helix 2 from 92 (out) to 109 (in) : 5.874 Transmembrane helix 3 from 113 (in) to 128 (out) : 1.963 Transmembrane helix 4 from 132 (out) to 147 (in) : 5.98 Transmembrane helix 5 from 151 (in) to 166 (out) : 0.352 Transmembrane helix 6 from 170 (out) to 185 (in) : 0 Transmembrane helix 7 from 189 (in) to 204 (out) : 0 Transmembrane helix 8 from 208 (out) to 223 (in) : 0 Re-entrant helix 1 from 35 (out) to 69 (out) : 2450 Re-entrant helix 2 from 70 (out) to 88 (out) : 1330 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 571.077 Transmembrane helix 2 from 63 (in) to 78 (out) : 7.576 Transmembrane helix 3 from 132 (out) to 147 (in) : 5.98 Transmembrane helix 4 from 151 (in) to 166 (out) : 0.352 Transmembrane helix 5 from 170 (out) to 185 (in) : 0 Transmembrane helix 6 from 189 (in) to 204 (out) : 0 Transmembrane helix 7 from 208 (out) to 223 (in) : 0 Re-entrant helix 1 from 35 (in) to 59 (in) : 1750 Re-entrant helix 2 from 82 (out) to 116 (out) : 2450 Re-entrant helix 3 from 117 (out) to 128 (out) : 840 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 588.723 Transmembrane helix 2 from 92 (out) to 109 (in) : 5.874 Transmembrane helix 3 from 113 (in) to 128 (out) : 1.963 Transmembrane helix 4 from 132 (out) to 147 (in) : 5.98 Transmembrane helix 5 from 151 (in) to 166 (out) : 0.352 Transmembrane helix 6 from 170 (out) to 185 (in) : 0 Re-entrant helix 1 from 35 (out) to 69 (out) : 2450 Re-entrant helix 2 from 70 (out) to 88 (out) : 1330 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 571.077 Transmembrane helix 2 from 63 (in) to 78 (out) : 7.576 Transmembrane helix 3 from 132 (out) to 147 (in) : 5.98 Transmembrane helix 4 from 151 (in) to 166 (out) : 0.352 Transmembrane helix 5 from 170 (out) to 185 (in) : 0 Re-entrant helix 1 from 35 (in) to 59 (in) : 1750 Re-entrant helix 2 from 82 (out) to 116 (out) : 2450 Re-entrant helix 3 from 117 (out) to 128 (out) : 840 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 588.723 Transmembrane helix 2 from 92 (out) to 112 (in) : 8.282 Transmembrane helix 3 from 116 (in) to 131 (out) : 2.625 Transmembrane helix 4 from 135 (out) to 151 (in) : 3.642 Re-entrant helix 1 from 35 (out) to 69 (out) : 2450 Re-entrant helix 2 from 70 (out) to 88 (out) : 1330 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 571.077 Transmembrane helix 2 from 63 (in) to 78 (out) : 7.576 Transmembrane helix 3 from 132 (out) to 151 (in) : 6.892 Re-entrant helix 1 from 35 (in) to 59 (in) : 1750 Re-entrant helix 2 from 82 (out) to 116 (out) : 2450 Re-entrant helix 3 from 117 (out) to 128 (out) : 840 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 588.723 Transmembrane helix 2 from 92 (out) to 122 (in) : 13.105 Re-entrant helix 1 from 35 (out) to 69 (out) : 2450 Re-entrant helix 2 from 70 (out) to 88 (out) : 1330 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.549584 1 helix (-) : Score = 0.610216 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 32.698 Topology: 16-31 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.610216