Processing 1 helix: Transmembrane helix 1 from 118 (in) to 133 (out) : -255.883 Score = -0.254441 Processing 1 helix: Transmembrane helix 1 from 118 (out) to 133 (in) : -270.917 Score = -0.272359 Processing 7 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -27479.4 Transmembrane helix 2 from 23 (out) to 38 (in) : -652.863 Transmembrane helix 3 from 42 (in) to 57 (out) : -967.302 Transmembrane helix 4 from 61 (out) to 76 (in) : -942.925 Transmembrane helix 5 from 80 (in) to 95 (out) : -545.79 Transmembrane helix 6 from 99 (out) to 114 (in) : -417.199 Transmembrane helix 7 from 118 (in) to 133 (out) : -255.883 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 23 (out) to 38 (in) : -652.863 Transmembrane helix 2 from 42 (in) to 57 (out) : -967.302 Transmembrane helix 3 from 61 (out) to 76 (in) : -942.925 Transmembrane helix 4 from 80 (in) to 95 (out) : -545.79 Transmembrane helix 5 from 99 (out) to 114 (in) : -417.199 Transmembrane helix 6 from 118 (in) to 133 (out) : -255.883 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 30 (in) to 45 (out) : -467.44 Transmembrane helix 2 from 61 (out) to 76 (in) : -942.925 Transmembrane helix 3 from 80 (in) to 95 (out) : -545.79 Transmembrane helix 4 from 99 (out) to 114 (in) : -417.199 Transmembrane helix 5 from 118 (in) to 133 (out) : -255.883 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 29 (out) to 44 (in) : -457.49 Transmembrane helix 2 from 80 (in) to 95 (out) : -545.79 Transmembrane helix 3 from 99 (out) to 114 (in) : -417.199 Transmembrane helix 4 from 118 (in) to 133 (out) : -255.883 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 80 (in) to 95 (out) : -545.79 Transmembrane helix 2 from 99 (out) to 114 (in) : -417.199 Transmembrane helix 3 from 118 (in) to 133 (out) : -255.883 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 29 (out) to 44 (in) : -457.49 Transmembrane helix 2 from 118 (in) to 133 (out) : -255.883 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -27455.3 Transmembrane helix 2 from 23 (in) to 38 (out) : -667.037 Transmembrane helix 3 from 42 (out) to 57 (in) : -975.598 Transmembrane helix 4 from 61 (in) to 76 (out) : -938.875 Transmembrane helix 5 from 80 (out) to 95 (in) : -544.71 Transmembrane helix 6 from 99 (in) to 114 (out) : -420.001 Transmembrane helix 7 from 118 (out) to 133 (in) : -270.917 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 23 (in) to 38 (out) : -667.037 Transmembrane helix 2 from 42 (out) to 57 (in) : -975.598 Transmembrane helix 3 from 61 (in) to 76 (out) : -938.875 Transmembrane helix 4 from 80 (out) to 95 (in) : -544.71 Transmembrane helix 5 from 99 (in) to 114 (out) : -420.001 Transmembrane helix 6 from 118 (out) to 133 (in) : -270.917 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 29 (out) to 44 (in) : -457.49 Transmembrane helix 2 from 61 (in) to 76 (out) : -938.875 Transmembrane helix 3 from 80 (out) to 95 (in) : -544.71 Transmembrane helix 4 from 99 (in) to 114 (out) : -420.001 Transmembrane helix 5 from 118 (out) to 133 (in) : -270.917 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 30 (in) to 45 (out) : -467.44 Transmembrane helix 2 from 80 (out) to 95 (in) : -544.71 Transmembrane helix 3 from 99 (in) to 114 (out) : -420.001 Transmembrane helix 4 from 118 (out) to 133 (in) : -270.917 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 29 (out) to 44 (in) : -457.49 Transmembrane helix 2 from 96 (in) to 111 (out) : -415.818 Transmembrane helix 3 from 118 (out) to 133 (in) : -270.917 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 96 (in) to 111 (out) : -415.818 Transmembrane helix 2 from 118 (out) to 133 (in) : -270.917 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.254441 1 helix (-) : Score = -0.272359 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 Results: Signal peptide: 1-18 Signal score: 60.399 Topology: 118-133 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: -0.254441