BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (96 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P76165 Uncharacterized protein ydfX n=44 Tax=Escherichi...   196   2e-49
UniRef50_B5R8N9 Predicted phage protein n=15 Tax=Salmonella ente...    83   2e-15
UniRef50_C8U4T3 Probable phage regulatory protein CII n=2 Tax=Es...    42   0.005

>UniRef50_P76165 Uncharacterized protein ydfX n=44 Tax=Escherichia
          RepID=YDFX_ECOLI
          Length = 96

 Score =  196 bits (497), Expect = 2e-49,   Method: Compositional matrix adjust.
 Identities = 96/96 (100%), Positives = 96/96 (100%)

Query: 1  MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRAL 60
          MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRAL
Sbjct: 1  MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRAL 60

Query: 61 GVNRVKIFERWKAIDTRDKREKFTALVPAIMEATTG 96
          GVNRVKIFERWKAIDTRDKREKFTALVPAIMEATTG
Sbjct: 61 GVNRVKIFERWKAIDTRDKREKFTALVPAIMEATTG 96


>UniRef50_B5R8N9 Predicted phage protein n=15 Tax=Salmonella enterica subsp.
          enterica RepID=B5R8N9_SALG2
          Length = 164

 Score = 83.2 bits (204), Expect = 2e-15,   Method: Compositional matrix adjust.
 Identities = 43/91 (47%), Positives = 57/91 (62%)

Query: 3  ITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRALGV 62
          ITPE A +AL +W+    +TQE AT LIT AF     RP I V R+  + G +D  A   
Sbjct: 2  ITPETASQALSSWLAYLQITQETATQLITRAFLEQPARPEIAVHRIERDDGTVDYDAWRR 61

Query: 63 NRVKIFERWKAIDTRDKREKFTALVPAIMEA 93
          NR+ IF+RW+  +T +  EKF+AL PAI+EA
Sbjct: 62 NRINIFQRWRKRETAEHCEKFSALTPAILEA 92


>UniRef50_C8U4T3 Probable phage regulatory protein CII n=2 Tax=Escherichia coli
          RepID=C8U4T3_ECO10
          Length = 145

 Score = 42.4 bits (98), Expect = 0.005,   Method: Compositional matrix adjust.
 Identities = 33/93 (35%), Positives = 47/93 (50%), Gaps = 12/93 (12%)

Query: 1  MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKER--PNIDVQRVTYEGGAIDQR 58
          MKI  E  R A++AW+  P + +++    I  A++ L+    P  D    T EG      
Sbjct: 1  MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPMHDTS--TTEG------ 52

Query: 59 ALGVNRVKIFERWKAIDTRDKREKFTALVPAIM 91
           +G+N   IF RW   DT D  EK  AL+PAI+
Sbjct: 53 -IGLNIQNIF-RWLEKDTPDAVEKIQALIPAIL 83


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P76165 Uncharacterized protein ydfX n=44 Tax=Escherichi...   154   1e-36
UniRef50_B5R8N9 Predicted phage protein n=15 Tax=Salmonella ente...   145   4e-34

Sequences not found previously or not previously below threshold:

UniRef50_C8U4T3 Probable phage regulatory protein CII n=2 Tax=Es...    47   2e-04
UniRef50_A7ZND8 Putative uncharacterized protein n=2 Tax=Escheri...    46   5e-04
UniRef50_B7UQF8 Probable phage regulatory protein n=95 Tax=Enter...    41   0.012
UniRef50_Q7N1J8 Similarities with unknown protein and with YdaT ...    38   0.099

>UniRef50_P76165 Uncharacterized protein ydfX n=44 Tax=Escherichia
          RepID=YDFX_ECOLI
          Length = 96

 Score =  154 bits (388), Expect = 1e-36,   Method: Composition-based stats.
 Identities = 96/96 (100%), Positives = 96/96 (100%)

Query: 1  MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRAL 60
          MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRAL
Sbjct: 1  MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRAL 60

Query: 61 GVNRVKIFERWKAIDTRDKREKFTALVPAIMEATTG 96
          GVNRVKIFERWKAIDTRDKREKFTALVPAIMEATTG
Sbjct: 61 GVNRVKIFERWKAIDTRDKREKFTALVPAIMEATTG 96


>UniRef50_B5R8N9 Predicted phage protein n=15 Tax=Salmonella enterica subsp.
          enterica RepID=B5R8N9_SALG2
          Length = 164

 Score =  145 bits (366), Expect = 4e-34,   Method: Composition-based stats.
 Identities = 43/91 (47%), Positives = 57/91 (62%)

Query: 3  ITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRALGV 62
          ITPE A +AL +W+    +TQE AT LIT AF     RP I V R+  + G +D  A   
Sbjct: 2  ITPETASQALSSWLAYLQITQETATQLITRAFLEQPARPEIAVHRIERDDGTVDYDAWRR 61

Query: 63 NRVKIFERWKAIDTRDKREKFTALVPAIMEA 93
          NR+ IF+RW+  +T +  EKF+AL PAI+EA
Sbjct: 62 NRINIFQRWRKRETAEHCEKFSALTPAILEA 92


>UniRef50_C8U4T3 Probable phage regulatory protein CII n=2 Tax=Escherichia coli
          RepID=C8U4T3_ECO10
          Length = 145

 Score = 47.0 bits (110), Expect = 2e-04,   Method: Composition-based stats.
 Identities = 30/91 (32%), Positives = 45/91 (49%), Gaps = 8/91 (8%)

Query: 1  MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRAL 60
          MKI  E  R A++AW+  P + +++    I  A++ L E     +   +   G      +
Sbjct: 1  MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFEL-EMTYPPMHDTSTTEG------I 53

Query: 61 GVNRVKIFERWKAIDTRDKREKFTALVPAIM 91
          G+N   IF RW   DT D  EK  AL+PAI+
Sbjct: 54 GLNIQNIF-RWLEKDTPDAVEKIQALIPAIL 83


>UniRef50_A7ZND8 Putative uncharacterized protein n=2 Tax=Escherichia coli
          RepID=A7ZND8_ECO24
          Length = 158

 Score = 45.9 bits (107), Expect = 5e-04,   Method: Composition-based stats.
 Identities = 28/89 (31%), Positives = 45/89 (50%), Gaps = 4/89 (4%)

Query: 5  PEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRALGVNR 64
          PE+ ++ +  W  R G  QE  TI I  A+++      + +       G +D RA+  NR
Sbjct: 4  PEELQKEILTWAARAG--QELVTIEICRAWFSQGRNDELRLHEFEDADGNVDWRAINNNR 61

Query: 65 VKIFERWKAIDTRDKREKFTALVPAIMEA 93
           KIF RW   +T   R K T ++ ++M+A
Sbjct: 62 QKIF-RWLRGETTAARRK-TQVLASVMKA 88


>UniRef50_B7UQF8 Probable phage regulatory protein n=95 Tax=Enterobacteriaceae
          RepID=B7UQF8_ECO27
          Length = 141

 Score = 40.9 bits (94), Expect = 0.012,   Method: Composition-based stats.
 Identities = 34/94 (36%), Positives = 44/94 (46%), Gaps = 12/94 (12%)

Query: 1  MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKER-PNIDVQRVTYEGGAIDQRA 59
          MKI  E  R A++ W   P   +  A   IT+A++ L    P +      Y+       A
Sbjct: 1  MKIKHEHIRMAMNVW-AHPDGEKVPAAK-ITKAYFELGMTFPEL------YDDS--HPEA 50

Query: 60 LGVNRVKIFERWKAIDTRDKREKFTALVPAIMEA 93
          L  N  KIF RW   DT D  EK  AL+PAI +A
Sbjct: 51 LARNTQKIF-RWLDKDTPDAVEKMQALLPAIEKA 83


>UniRef50_Q7N1J8 Similarities with unknown protein and with YdaT protein of
          Escherichia coli n=5 Tax=Enterobacteriaceae
          RepID=Q7N1J8_PHOLL
          Length = 149

 Score = 37.8 bits (86), Expect = 0.099,   Method: Composition-based stats.
 Identities = 28/83 (33%), Positives = 43/83 (51%), Gaps = 5/83 (6%)

Query: 12 LDAWICRPGMTQEQATILITEAFWALKERP-NIDVQRVTYEGGAIDQRALGVNRVKIFER 70
          ++AW    G  QE   I I+  ++ L ER  +  +  +   G A + +A+  NR +IF R
Sbjct: 12 VEAWAAEKG--QEYVAIEISRMYFLLCERTVSAKLHPIEINGNA-NWKAINNNRQQIF-R 67

Query: 71 WKAIDTRDKREKFTALVPAIMEA 93
          W   D+R  R K + L+PAI  A
Sbjct: 68 WLRSDSRAARRKVSELLPAIQSA 90


Searching..................................................done


Results from round 3


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P76165 Uncharacterized protein ydfX n=44 Tax=Escherichi...   135   4e-31
UniRef50_B5R8N9 Predicted phage protein n=15 Tax=Salmonella ente...   126   2e-28
UniRef50_C8U4T3 Probable phage regulatory protein CII n=2 Tax=Es...   115   4e-25
UniRef50_A7ZND8 Putative uncharacterized protein n=2 Tax=Escheri...   104   6e-22

Sequences not found previously or not previously below threshold:

UniRef50_B7UQF8 Probable phage regulatory protein n=95 Tax=Enter...    77   2e-13
UniRef50_Q7N1J8 Similarities with unknown protein and with YdaT ...    68   9e-11
UniRef50_C0AW01 Putative uncharacterized protein n=2 Tax=Enterob...    66   4e-10
UniRef50_P76064 Uncharacterized protein ydaT n=20 Tax=Enterobact...    57   2e-07
UniRef50_C4SVT0 tRNA-(Guanine-N1)-methyltransferase n=1 Tax=Yers...    53   3e-06
UniRef50_B4T224 Putative uncharacterized protein n=2 Tax=Salmone...    51   2e-05

>UniRef50_P76165 Uncharacterized protein ydfX n=44 Tax=Escherichia
          RepID=YDFX_ECOLI
          Length = 96

 Score =  135 bits (339), Expect = 4e-31,   Method: Composition-based stats.
 Identities = 96/96 (100%), Positives = 96/96 (100%)

Query: 1  MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRAL 60
          MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRAL
Sbjct: 1  MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRAL 60

Query: 61 GVNRVKIFERWKAIDTRDKREKFTALVPAIMEATTG 96
          GVNRVKIFERWKAIDTRDKREKFTALVPAIMEATTG
Sbjct: 61 GVNRVKIFERWKAIDTRDKREKFTALVPAIMEATTG 96


>UniRef50_B5R8N9 Predicted phage protein n=15 Tax=Salmonella enterica subsp.
          enterica RepID=B5R8N9_SALG2
          Length = 164

 Score =  126 bits (317), Expect = 2e-28,   Method: Composition-based stats.
 Identities = 43/91 (47%), Positives = 57/91 (62%)

Query: 3  ITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRALGV 62
          ITPE A +AL +W+    +TQE AT LIT AF     RP I V R+  + G +D  A   
Sbjct: 2  ITPETASQALSSWLAYLQITQETATQLITRAFLEQPARPEIAVHRIERDDGTVDYDAWRR 61

Query: 63 NRVKIFERWKAIDTRDKREKFTALVPAIMEA 93
          NR+ IF+RW+  +T +  EKF+AL PAI+EA
Sbjct: 62 NRINIFQRWRKRETAEHCEKFSALTPAILEA 92


>UniRef50_C8U4T3 Probable phage regulatory protein CII n=2 Tax=Escherichia coli
          RepID=C8U4T3_ECO10
          Length = 145

 Score =  115 bits (287), Expect = 4e-25,   Method: Composition-based stats.
 Identities = 30/91 (32%), Positives = 45/91 (49%), Gaps = 8/91 (8%)

Query: 1  MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRAL 60
          MKI  E  R A++AW+  P + +++    I  A++ L E     +   +   G      +
Sbjct: 1  MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFEL-EMTYPPMHDTSTTEG------I 53

Query: 61 GVNRVKIFERWKAIDTRDKREKFTALVPAIM 91
          G+N   IF RW   DT D  EK  AL+PAI+
Sbjct: 54 GLNIQNIF-RWLEKDTPDAVEKIQALIPAIL 83


>UniRef50_A7ZND8 Putative uncharacterized protein n=2 Tax=Escherichia coli
          RepID=A7ZND8_ECO24
          Length = 158

 Score =  104 bits (260), Expect = 6e-22,   Method: Composition-based stats.
 Identities = 28/89 (31%), Positives = 45/89 (50%), Gaps = 4/89 (4%)

Query: 5  PEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRALGVNR 64
          PE+ ++ +  W  R G  QE  TI I  A+++      + +       G +D RA+  NR
Sbjct: 4  PEELQKEILTWAARAG--QELVTIEICRAWFSQGRNDELRLHEFEDADGNVDWRAINNNR 61

Query: 65 VKIFERWKAIDTRDKREKFTALVPAIMEA 93
           KIF RW   +T   R K T ++ ++M+A
Sbjct: 62 QKIF-RWLRGETTAARRK-TQVLASVMKA 88


>UniRef50_B7UQF8 Probable phage regulatory protein n=95 Tax=Enterobacteriaceae
          RepID=B7UQF8_ECO27
          Length = 141

 Score = 76.8 bits (187), Expect = 2e-13,   Method: Composition-based stats.
 Identities = 31/93 (33%), Positives = 44/93 (47%), Gaps = 10/93 (10%)

Query: 1  MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRAL 60
          MKI  E  R A++ W   P   ++     IT+A++ L      ++   ++        AL
Sbjct: 1  MKIKHEHIRMAMNVW-AHPD-GEKVPAAKITKAYFELG-MTFPELYDDSHPE------AL 51

Query: 61 GVNRVKIFERWKAIDTRDKREKFTALVPAIMEA 93
            N  KIF RW   DT D  EK  AL+PAI +A
Sbjct: 52 ARNTQKIF-RWLDKDTPDAVEKMQALLPAIEKA 83


>UniRef50_Q7N1J8 Similarities with unknown protein and with YdaT protein of
          Escherichia coli n=5 Tax=Enterobacteriaceae
          RepID=Q7N1J8_PHOLL
          Length = 149

 Score = 68.0 bits (164), Expect = 9e-11,   Method: Composition-based stats.
 Identities = 27/87 (31%), Positives = 43/87 (49%), Gaps = 5/87 (5%)

Query: 8  AREALDAWICRPGMTQEQATILITEAFWALKERP-NIDVQRVTYEGGAIDQRALGVNRVK 66
           +  ++AW    G  QE   I I+  ++ L ER  +  +  +    G  + +A+  NR +
Sbjct: 8  LKAEVEAWAAEKG--QEYVAIEISRMYFLLCERTVSAKLHPIE-INGNANWKAINNNRQQ 64

Query: 67 IFERWKAIDTRDKREKFTALVPAIMEA 93
          IF RW   D+R  R K + L+PAI  A
Sbjct: 65 IF-RWLRSDSRAARRKVSELLPAIQSA 90


>UniRef50_C0AW01 Putative uncharacterized protein n=2 Tax=Enterobacteriaceae
          RepID=C0AW01_9ENTR
          Length = 151

 Score = 65.7 bits (158), Expect = 4e-10,   Method: Composition-based stats.
 Identities = 25/84 (29%), Positives = 38/84 (45%), Gaps = 4/84 (4%)

Query: 8  AREALDAWICRPGMTQEQATILITEAFWALKERPNI-DVQRVTYEGGAIDQRALGVNRVK 66
           R  ++ W    G  QE   I I+ A+  L        +  +  + G  D +A+  NR +
Sbjct: 8  IRAEIEDWAVEQG--QEHVAIEISRAYLRLVINQEHGRLHVIEDQTGRADWKAINNNRQQ 65

Query: 67 IFERWKAIDTRDKREKFTALVPAI 90
          IF RW   D+R  + K   L+PAI
Sbjct: 66 IF-RWLRGDSRASQRKIAELMPAI 88


>UniRef50_P76064 Uncharacterized protein ydaT n=20 Tax=Enterobacteriaceae
          RepID=YDAT_ECOLI
          Length = 140

 Score = 56.8 bits (135), Expect = 2e-07,   Method: Composition-based stats.
 Identities = 26/93 (27%), Positives = 35/93 (37%), Gaps = 17/93 (18%)

Query: 1  MKITPEQAREALDAWICRPGMTQEQATILITEAFWALK--ERPNIDVQRVTYEGGAIDQR 58
          MKI  E     L A     G  Q      ITE +      E P +            D  
Sbjct: 1  MKIKHEHIESVLFALAAEKG--QAWVANAITEEYLRQGGGELPLVP---------GKDW- 48

Query: 59 ALGVNRVKIFERWKAIDTRDKREKFTALVPAIM 91
              N+  I+ RW   +T+ +REK   L+PAI+
Sbjct: 49 ---NNQQNIYHRWLKGETKTQREKIQKLIPAIL 78


>UniRef50_C4SVT0 tRNA-(Guanine-N1)-methyltransferase n=1 Tax=Yersinia
          frederiksenii ATCC 33641 RepID=C4SVT0_YERFR
          Length = 146

 Score = 53.0 bits (125), Expect = 3e-06,   Method: Composition-based stats.
 Identities = 25/96 (26%), Positives = 38/96 (39%), Gaps = 10/96 (10%)

Query: 1  MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNIDVQRVTYEGGAIDQRAL 60
          MK+  +     L  W       QE     I +A++ L     + +  V       D+ A 
Sbjct: 1  MKLKHDAICAELRGWAA--ETKQEIVAAEIAQAYFVLGGGD-LPLTPVN------DEHAT 51

Query: 61 GVNRVKIFERWKAIDTRDKREKFTALVPAIMEATTG 96
            N+ ++F RW   DT   + K   L PAI+ A  G
Sbjct: 52 HNNKQRLF-RWIDSDTDRSKTKIAELTPAILRALPG 86


>UniRef50_B4T224 Putative uncharacterized protein n=2 Tax=Salmonella enterica
          subsp. enterica RepID=B4T224_SALNS
          Length = 115

 Score = 50.6 bits (119), Expect = 2e-05,   Method: Composition-based stats.
 Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 1/51 (1%)

Query: 43 IDVQRVTYEGGAIDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEA 93
          + + ++    G  D RA+  NR +IF RW   +T+  R K  AL  A+  A
Sbjct: 8  VKLHQMEDSKGNADWRAINNNRQQIF-RWLRGETKAARTKTKALAKAMEAA 57


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.301    0.117    0.299 

Lambda     K      H
   0.267   0.0359    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 464,119,236
Number of Sequences: 3077464
Number of extensions: 12681404
Number of successful extensions: 33574
Number of sequences better than 1.0e-01: 10
Number of HSP's better than  0.1 without gapping: 11
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 33551
Number of HSP's gapped (non-prelim): 21
length of query: 96
length of database: 1,040,396,356
effective HSP length: 65
effective length of query: 31
effective length of database: 840,361,196
effective search space: 26051197076
effective search space used: 26051197076
T: 11
A: 40
X1: 16 ( 6.9 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.9 bits)
S2: 86 (37.9 bits)