BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (32 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_C1P602 Uncharacterized membrane protein yoaK n=14 Tax=E...    61   1e-08
UniRef50_C4X6Y1 Putative uncharacterized protein n=6 Tax=Enterob...    52   7e-06

>UniRef50_C1P602 Uncharacterized membrane protein yoaK n=14 Tax=Enterobacteriaceae
          RepID=YOAK_ECOLI
          Length = 32

 Score = 60.8 bits (146), Expect = 1e-08,   Method: Compositional matrix adjust.
 Identities = 32/32 (100%), Positives = 32/32 (100%)

Query: 1  MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA 32
          MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA
Sbjct: 1  MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA 32


>UniRef50_C4X6Y1 Putative uncharacterized protein n=6 Tax=Enterobacteriaceae
          RepID=C4X6Y1_KLEPN
          Length = 45

 Score = 51.6 bits (122), Expect = 7e-06,   Method: Compositional matrix adjust.
 Identities = 24/32 (75%), Positives = 28/32 (87%)

Query: 1  MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA 32
          M++GI+FPV IFI AVVFL WFF+GGYAAPG 
Sbjct: 13 MKLGILFPVAIFIIAVVFLGWFFVGGYAAPGG 44


Searching..................................................done



 ***** No hits found ******

  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.332    0.163    0.576 

Lambda     K      H
   0.267   0.0516    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 169,971,240
Number of Sequences: 3077464
Number of extensions: 3737240
Number of successful extensions: 32875
Number of sequences better than 1.0e-01: 3
Number of HSP's better than  0.1 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 32869
Number of HSP's gapped (non-prelim): 5
length of query: 32
length of database: 1,040,396,356
effective HSP length: 7
effective length of query: 25
effective length of database: 1,018,854,108
effective search space: 25471352700
effective search space used: 25471352700
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.3 bits)
S2: 88 (38.2 bits)