Processing 1 helix: Transmembrane helix 1 from 111 (in) to 126 (out) : 138.445 Score = 0.138445 Processing 1 helix: Transmembrane helix 1 from 111 (out) to 126 (in) : 122.455 Score = 0.122455 Processing 22 helices: Transmembrane helix 1 from 17 (in) to 32 (out) : -2294.18 Transmembrane helix 2 from 36 (out) to 51 (in) : -887.008 Transmembrane helix 3 from 55 (in) to 70 (out) : -787.141 Transmembrane helix 4 from 74 (out) to 89 (in) : -1107.39 Transmembrane helix 5 from 93 (in) to 108 (out) : -633.371 Transmembrane helix 6 from 112 (out) to 127 (in) : 117.776 Transmembrane helix 7 from 131 (in) to 146 (out) : -1097.05 Transmembrane helix 8 from 150 (out) to 165 (in) : -946.26 Transmembrane helix 9 from 169 (in) to 184 (out) : -929.327 Transmembrane helix 10 from 188 (out) to 203 (in) : -710.267 Transmembrane helix 11 from 207 (in) to 222 (out) : -999.498 Transmembrane helix 12 from 226 (out) to 241 (in) : -358.077 Transmembrane helix 13 from 245 (in) to 260 (out) : -304.223 Transmembrane helix 14 from 264 (out) to 279 (in) : -1151.86 Transmembrane helix 15 from 283 (in) to 298 (out) : -1044.64 Transmembrane helix 16 from 302 (out) to 317 (in) : -905.663 Transmembrane helix 17 from 321 (in) to 336 (out) : -1096.46 Transmembrane helix 18 from 340 (out) to 355 (in) : -1030.11 Transmembrane helix 19 from 359 (in) to 374 (out) : -1143.95 Transmembrane helix 20 from 378 (out) to 393 (in) : -220.413 Transmembrane helix 21 from 397 (in) to 412 (out) : -211.992 Transmembrane helix 22 from 416 (out) to 431 (in) : -675.465 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 21 (out) to 36 (in) : -1038.05 Transmembrane helix 2 from 41 (in) to 56 (out) : -849.214 Transmembrane helix 3 from 60 (out) to 75 (in) : -845.176 Transmembrane helix 4 from 80 (in) to 95 (out) : -1093.3 Transmembrane helix 5 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 6 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 7 from 139 (out) to 154 (in) : -1180.56 Transmembrane helix 8 from 158 (in) to 173 (out) : -966.003 Transmembrane helix 9 from 177 (out) to 192 (in) : -778.777 Transmembrane helix 10 from 196 (in) to 211 (out) : -899.412 Transmembrane helix 11 from 215 (out) to 230 (in) : -882.791 Transmembrane helix 12 from 234 (in) to 249 (out) : -56.489 Transmembrane helix 13 from 253 (out) to 268 (in) : -679.485 Transmembrane helix 14 from 272 (in) to 287 (out) : -1185.56 Transmembrane helix 15 from 291 (out) to 306 (in) : -941.616 Transmembrane helix 16 from 310 (in) to 325 (out) : -1005.33 Transmembrane helix 17 from 331 (out) to 346 (in) : -755.353 Transmembrane helix 18 from 359 (in) to 374 (out) : -1143.95 Transmembrane helix 19 from 378 (out) to 393 (in) : -220.413 Transmembrane helix 20 from 397 (in) to 412 (out) : -211.992 Transmembrane helix 21 from 416 (out) to 431 (in) : -675.465 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 21 (in) to 36 (out) : -1043.25 Transmembrane helix 2 from 40 (out) to 55 (in) : -844.663 Transmembrane helix 3 from 59 (in) to 74 (out) : -830.15 Transmembrane helix 4 from 80 (out) to 95 (in) : -1083.8 Transmembrane helix 5 from 99 (in) to 114 (out) : -372.334 Transmembrane helix 6 from 118 (out) to 133 (in) : -30.769 Transmembrane helix 7 from 150 (in) to 165 (out) : -939.94 Transmembrane helix 8 from 169 (out) to 184 (in) : -928.373 Transmembrane helix 9 from 188 (in) to 203 (out) : -705.233 Transmembrane helix 10 from 207 (out) to 222 (in) : -998.602 Transmembrane helix 11 from 227 (in) to 242 (out) : -294.468 Transmembrane helix 12 from 246 (out) to 261 (in) : -345.393 Transmembrane helix 13 from 265 (in) to 280 (out) : -1156.51 Transmembrane helix 14 from 288 (out) to 303 (in) : -962.069 Transmembrane helix 15 from 307 (in) to 322 (out) : -938.743 Transmembrane helix 16 from 331 (out) to 346 (in) : -755.353 Transmembrane helix 17 from 359 (in) to 374 (out) : -1143.95 Transmembrane helix 18 from 378 (out) to 393 (in) : -220.413 Transmembrane helix 19 from 397 (in) to 412 (out) : -211.992 Transmembrane helix 20 from 416 (out) to 431 (in) : -675.465 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 21 (out) to 36 (in) : -1038.05 Transmembrane helix 2 from 41 (in) to 56 (out) : -849.214 Transmembrane helix 3 from 60 (out) to 75 (in) : -845.176 Transmembrane helix 4 from 80 (in) to 95 (out) : -1093.3 Transmembrane helix 5 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 6 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 7 from 151 (out) to 166 (in) : -936.431 Transmembrane helix 8 from 172 (in) to 187 (out) : -858.911 Transmembrane helix 9 from 191 (out) to 206 (in) : -763.243 Transmembrane helix 10 from 214 (in) to 229 (out) : -904.335 Transmembrane helix 11 from 233 (out) to 248 (in) : -91.197 Transmembrane helix 12 from 252 (in) to 267 (out) : -622.147 Transmembrane helix 13 from 288 (out) to 303 (in) : -962.069 Transmembrane helix 14 from 307 (in) to 322 (out) : -938.743 Transmembrane helix 15 from 331 (out) to 346 (in) : -755.353 Transmembrane helix 16 from 359 (in) to 374 (out) : -1143.95 Transmembrane helix 17 from 378 (out) to 393 (in) : -220.413 Transmembrane helix 18 from 397 (in) to 412 (out) : -211.992 Transmembrane helix 19 from 416 (out) to 431 (in) : -675.465 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 21 (in) to 36 (out) : -1043.25 Transmembrane helix 2 from 40 (out) to 55 (in) : -844.663 Transmembrane helix 3 from 59 (in) to 74 (out) : -830.15 Transmembrane helix 4 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 5 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 6 from 151 (out) to 166 (in) : -936.431 Transmembrane helix 7 from 172 (in) to 187 (out) : -858.911 Transmembrane helix 8 from 191 (out) to 206 (in) : -763.243 Transmembrane helix 9 from 214 (in) to 229 (out) : -904.335 Transmembrane helix 10 from 233 (out) to 248 (in) : -91.197 Transmembrane helix 11 from 252 (in) to 267 (out) : -622.147 Transmembrane helix 12 from 288 (out) to 303 (in) : -962.069 Transmembrane helix 13 from 307 (in) to 322 (out) : -938.743 Transmembrane helix 14 from 331 (out) to 346 (in) : -755.353 Transmembrane helix 15 from 359 (in) to 374 (out) : -1143.95 Transmembrane helix 16 from 378 (out) to 393 (in) : -220.413 Transmembrane helix 17 from 397 (in) to 412 (out) : -211.992 Transmembrane helix 18 from 416 (out) to 431 (in) : -675.465 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 21 (out) to 36 (in) : -1038.05 Transmembrane helix 2 from 41 (in) to 56 (out) : -849.214 Transmembrane helix 3 from 60 (out) to 75 (in) : -845.176 Transmembrane helix 4 from 80 (in) to 95 (out) : -1093.3 Transmembrane helix 5 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 6 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 7 from 151 (out) to 166 (in) : -936.431 Transmembrane helix 8 from 172 (in) to 187 (out) : -858.911 Transmembrane helix 9 from 191 (out) to 206 (in) : -763.243 Transmembrane helix 10 from 214 (in) to 229 (out) : -904.335 Transmembrane helix 11 from 233 (out) to 248 (in) : -91.197 Transmembrane helix 12 from 252 (in) to 267 (out) : -622.147 Transmembrane helix 13 from 304 (out) to 319 (in) : -892.411 Transmembrane helix 14 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 15 from 378 (out) to 393 (in) : -220.413 Transmembrane helix 16 from 397 (in) to 412 (out) : -211.992 Transmembrane helix 17 from 416 (out) to 431 (in) : -675.465 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 21 (in) to 36 (out) : -1043.25 Transmembrane helix 2 from 40 (out) to 55 (in) : -844.663 Transmembrane helix 3 from 59 (in) to 74 (out) : -830.15 Transmembrane helix 4 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 5 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 6 from 151 (out) to 166 (in) : -936.431 Transmembrane helix 7 from 172 (in) to 187 (out) : -858.911 Transmembrane helix 8 from 191 (out) to 206 (in) : -763.243 Transmembrane helix 9 from 214 (in) to 229 (out) : -904.335 Transmembrane helix 10 from 233 (out) to 248 (in) : -91.197 Transmembrane helix 11 from 252 (in) to 267 (out) : -622.147 Transmembrane helix 12 from 304 (out) to 319 (in) : -892.411 Transmembrane helix 13 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 14 from 378 (out) to 393 (in) : -220.413 Transmembrane helix 15 from 397 (in) to 412 (out) : -211.992 Transmembrane helix 16 from 416 (out) to 431 (in) : -675.465 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 38 (out) to 53 (in) : -859.234 Transmembrane helix 2 from 57 (in) to 72 (out) : -807.795 Transmembrane helix 3 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 4 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 5 from 151 (out) to 166 (in) : -936.431 Transmembrane helix 6 from 172 (in) to 187 (out) : -858.911 Transmembrane helix 7 from 191 (out) to 206 (in) : -763.243 Transmembrane helix 8 from 214 (in) to 229 (out) : -904.335 Transmembrane helix 9 from 233 (out) to 248 (in) : -91.197 Transmembrane helix 10 from 252 (in) to 267 (out) : -622.147 Transmembrane helix 11 from 304 (out) to 319 (in) : -892.411 Transmembrane helix 12 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 13 from 378 (out) to 393 (in) : -220.413 Transmembrane helix 14 from 397 (in) to 412 (out) : -211.992 Transmembrane helix 15 from 416 (out) to 431 (in) : -675.465 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 21 (in) to 36 (out) : -1043.25 Transmembrane helix 2 from 40 (out) to 55 (in) : -844.663 Transmembrane helix 3 from 59 (in) to 74 (out) : -830.15 Transmembrane helix 4 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 5 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 6 from 160 (out) to 175 (in) : -935.534 Transmembrane helix 7 from 183 (in) to 198 (out) : -670.359 Transmembrane helix 8 from 228 (out) to 243 (in) : -243.947 Transmembrane helix 9 from 247 (in) to 262 (out) : -378.793 Transmembrane helix 10 from 304 (out) to 319 (in) : -892.411 Transmembrane helix 11 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 12 from 378 (out) to 393 (in) : -220.413 Transmembrane helix 13 from 397 (in) to 412 (out) : -211.992 Transmembrane helix 14 from 416 (out) to 431 (in) : -675.465 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 38 (out) to 53 (in) : -859.234 Transmembrane helix 2 from 57 (in) to 72 (out) : -807.795 Transmembrane helix 3 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 4 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 5 from 160 (out) to 175 (in) : -935.534 Transmembrane helix 6 from 183 (in) to 198 (out) : -670.359 Transmembrane helix 7 from 228 (out) to 243 (in) : -243.947 Transmembrane helix 8 from 247 (in) to 262 (out) : -378.793 Transmembrane helix 9 from 304 (out) to 319 (in) : -892.411 Transmembrane helix 10 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 11 from 378 (out) to 393 (in) : -220.413 Transmembrane helix 12 from 397 (in) to 412 (out) : -211.992 Transmembrane helix 13 from 416 (out) to 431 (in) : -675.465 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 49 (in) to 64 (out) : -741.022 Transmembrane helix 2 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 3 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 4 from 160 (out) to 175 (in) : -935.534 Transmembrane helix 5 from 183 (in) to 198 (out) : -670.359 Transmembrane helix 6 from 228 (out) to 243 (in) : -243.947 Transmembrane helix 7 from 247 (in) to 262 (out) : -378.793 Transmembrane helix 8 from 304 (out) to 319 (in) : -892.411 Transmembrane helix 9 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 10 from 378 (out) to 393 (in) : -220.413 Transmembrane helix 11 from 397 (in) to 412 (out) : -211.992 Transmembrane helix 12 from 416 (out) to 431 (in) : -675.465 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 38 (out) to 53 (in) : -859.234 Transmembrane helix 2 from 57 (in) to 72 (out) : -807.795 Transmembrane helix 3 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 4 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 5 from 183 (out) to 198 (in) : -675.941 Transmembrane helix 6 from 228 (in) to 243 (out) : -239.853 Transmembrane helix 7 from 247 (out) to 262 (in) : -388.107 Transmembrane helix 8 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 9 from 378 (out) to 393 (in) : -220.413 Transmembrane helix 10 from 397 (in) to 412 (out) : -211.992 Transmembrane helix 11 from 416 (out) to 431 (in) : -675.465 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 49 (in) to 64 (out) : -741.022 Transmembrane helix 2 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 3 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 4 from 183 (out) to 198 (in) : -675.941 Transmembrane helix 5 from 228 (in) to 243 (out) : -239.853 Transmembrane helix 6 from 247 (out) to 262 (in) : -388.107 Transmembrane helix 7 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 8 from 378 (out) to 393 (in) : -220.413 Transmembrane helix 9 from 397 (in) to 412 (out) : -211.992 Transmembrane helix 10 from 416 (out) to 431 (in) : -675.465 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 2 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 3 from 183 (out) to 198 (in) : -675.941 Transmembrane helix 4 from 228 (in) to 243 (out) : -239.853 Transmembrane helix 5 from 247 (out) to 262 (in) : -388.107 Transmembrane helix 6 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 7 from 378 (out) to 393 (in) : -220.413 Transmembrane helix 8 from 397 (in) to 412 (out) : -211.992 Transmembrane helix 9 from 416 (out) to 431 (in) : -675.465 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 49 (in) to 64 (out) : -741.022 Transmembrane helix 2 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 3 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 4 from 183 (out) to 198 (in) : -675.941 Transmembrane helix 5 from 228 (in) to 243 (out) : -239.853 Transmembrane helix 6 from 247 (out) to 262 (in) : -388.107 Transmembrane helix 7 from 383 (in) to 398 (out) : 5.88 Transmembrane helix 8 from 402 (out) to 417 (in) : -304.83 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 2 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 3 from 183 (out) to 198 (in) : -675.941 Transmembrane helix 4 from 228 (in) to 243 (out) : -239.853 Transmembrane helix 5 from 247 (out) to 262 (in) : -388.107 Transmembrane helix 6 from 383 (in) to 398 (out) : 5.88 Transmembrane helix 7 from 402 (out) to 417 (in) : -304.83 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 99 (in) to 114 (out) : -372.334 Transmembrane helix 2 from 118 (out) to 133 (in) : -30.769 Transmembrane helix 3 from 228 (in) to 243 (out) : -239.853 Transmembrane helix 4 from 247 (out) to 262 (in) : -388.107 Transmembrane helix 5 from 383 (in) to 398 (out) : 5.88 Transmembrane helix 6 from 402 (out) to 417 (in) : -304.83 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 2 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 3 from 236 (out) to 251 (in) : -31.003 Transmembrane helix 4 from 383 (in) to 398 (out) : 5.88 Transmembrane helix 5 from 402 (out) to 417 (in) : -304.83 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 111 (in) to 126 (out) : 138.445 Transmembrane helix 2 from 236 (out) to 251 (in) : -31.003 Transmembrane helix 3 from 383 (in) to 398 (out) : 5.88 Transmembrane helix 4 from 402 (out) to 417 (in) : -304.83 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 111 (out) to 126 (in) : 122.455 Transmembrane helix 2 from 236 (in) to 251 (out) : -28.597 Transmembrane helix 3 from 387 (out) to 402 (in) : 14.274 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 111 (in) to 126 (out) : 138.445 Transmembrane helix 2 from 387 (out) to 402 (in) : 14.274 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 17 (out) to 32 (in) : -2290.82 Transmembrane helix 2 from 36 (in) to 51 (out) : -903.192 Transmembrane helix 3 from 55 (out) to 70 (in) : -798.859 Transmembrane helix 4 from 74 (in) to 89 (out) : -1106.31 Transmembrane helix 5 from 93 (out) to 108 (in) : -636.329 Transmembrane helix 6 from 112 (in) to 127 (out) : 132.924 Transmembrane helix 7 from 131 (out) to 146 (in) : -1083.45 Transmembrane helix 8 from 150 (in) to 165 (out) : -939.94 Transmembrane helix 9 from 169 (out) to 184 (in) : -928.373 Transmembrane helix 10 from 188 (in) to 203 (out) : -705.233 Transmembrane helix 11 from 207 (out) to 222 (in) : -998.602 Transmembrane helix 12 from 226 (in) to 241 (out) : -358.923 Transmembrane helix 13 from 245 (out) to 260 (in) : -314.077 Transmembrane helix 14 from 264 (in) to 279 (out) : -1145.84 Transmembrane helix 15 from 283 (out) to 298 (in) : -1041.96 Transmembrane helix 16 from 302 (in) to 317 (out) : -907.237 Transmembrane helix 17 from 321 (out) to 336 (in) : -1100.44 Transmembrane helix 18 from 340 (in) to 355 (out) : -1019.69 Transmembrane helix 19 from 359 (out) to 374 (in) : -1144.45 Transmembrane helix 20 from 378 (in) to 393 (out) : -224.287 Transmembrane helix 21 from 397 (out) to 412 (in) : -213.008 Transmembrane helix 22 from 416 (in) to 431 (out) : -678.135 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 21 (in) to 36 (out) : -1043.25 Transmembrane helix 2 from 40 (out) to 55 (in) : -844.663 Transmembrane helix 3 from 59 (in) to 74 (out) : -830.15 Transmembrane helix 4 from 80 (out) to 95 (in) : -1083.8 Transmembrane helix 5 from 99 (in) to 114 (out) : -372.334 Transmembrane helix 6 from 118 (out) to 133 (in) : -30.769 Transmembrane helix 7 from 138 (in) to 153 (out) : -1195.02 Transmembrane helix 8 from 158 (out) to 173 (in) : -951.297 Transmembrane helix 9 from 177 (in) to 192 (out) : -775.823 Transmembrane helix 10 from 196 (out) to 211 (in) : -889.188 Transmembrane helix 11 from 215 (in) to 230 (out) : -880.909 Transmembrane helix 12 from 234 (out) to 249 (in) : -61.011 Transmembrane helix 13 from 253 (in) to 268 (out) : -682.515 Transmembrane helix 14 from 272 (out) to 287 (in) : -1184.44 Transmembrane helix 15 from 291 (in) to 306 (out) : -953.784 Transmembrane helix 16 from 310 (out) to 325 (in) : -1008.87 Transmembrane helix 17 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 18 from 359 (out) to 374 (in) : -1144.45 Transmembrane helix 19 from 378 (in) to 393 (out) : -224.287 Transmembrane helix 20 from 397 (out) to 412 (in) : -213.008 Transmembrane helix 21 from 416 (in) to 431 (out) : -678.135 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 21 (out) to 36 (in) : -1038.05 Transmembrane helix 2 from 41 (in) to 56 (out) : -849.214 Transmembrane helix 3 from 60 (out) to 75 (in) : -845.176 Transmembrane helix 4 from 80 (in) to 95 (out) : -1093.3 Transmembrane helix 5 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 6 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 7 from 151 (out) to 166 (in) : -936.431 Transmembrane helix 8 from 172 (in) to 187 (out) : -858.911 Transmembrane helix 9 from 191 (out) to 206 (in) : -763.243 Transmembrane helix 10 from 210 (in) to 225 (out) : -994.745 Transmembrane helix 11 from 229 (out) to 244 (in) : -209.222 Transmembrane helix 12 from 248 (in) to 263 (out) : -427.728 Transmembrane helix 13 from 267 (out) to 282 (in) : -1187.98 Transmembrane helix 14 from 288 (in) to 303 (out) : -971.331 Transmembrane helix 15 from 307 (out) to 322 (in) : -936.357 Transmembrane helix 16 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 17 from 359 (out) to 374 (in) : -1144.45 Transmembrane helix 18 from 378 (in) to 393 (out) : -224.287 Transmembrane helix 19 from 397 (out) to 412 (in) : -213.008 Transmembrane helix 20 from 416 (in) to 431 (out) : -678.135 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 21 (in) to 36 (out) : -1043.25 Transmembrane helix 2 from 40 (out) to 55 (in) : -844.663 Transmembrane helix 3 from 59 (in) to 74 (out) : -830.15 Transmembrane helix 4 from 80 (out) to 95 (in) : -1083.8 Transmembrane helix 5 from 99 (in) to 114 (out) : -372.334 Transmembrane helix 6 from 118 (out) to 133 (in) : -30.769 Transmembrane helix 7 from 151 (in) to 166 (out) : -932.069 Transmembrane helix 8 from 172 (out) to 187 (in) : -860.689 Transmembrane helix 9 from 191 (in) to 206 (out) : -767.457 Transmembrane helix 10 from 214 (out) to 229 (in) : -906.765 Transmembrane helix 11 from 233 (in) to 248 (out) : -84.703 Transmembrane helix 12 from 252 (out) to 267 (in) : -620.453 Transmembrane helix 13 from 288 (in) to 303 (out) : -971.331 Transmembrane helix 14 from 307 (out) to 322 (in) : -936.357 Transmembrane helix 15 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 16 from 359 (out) to 374 (in) : -1144.45 Transmembrane helix 17 from 378 (in) to 393 (out) : -224.287 Transmembrane helix 18 from 397 (out) to 412 (in) : -213.008 Transmembrane helix 19 from 416 (in) to 431 (out) : -678.135 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 21 (out) to 36 (in) : -1038.05 Transmembrane helix 2 from 41 (in) to 56 (out) : -849.214 Transmembrane helix 3 from 60 (out) to 75 (in) : -845.176 Transmembrane helix 4 from 80 (in) to 95 (out) : -1093.3 Transmembrane helix 5 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 6 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 7 from 151 (out) to 166 (in) : -936.431 Transmembrane helix 8 from 172 (in) to 187 (out) : -858.911 Transmembrane helix 9 from 191 (out) to 206 (in) : -763.243 Transmembrane helix 10 from 214 (in) to 229 (out) : -904.335 Transmembrane helix 11 from 233 (out) to 248 (in) : -91.197 Transmembrane helix 12 from 252 (in) to 267 (out) : -622.147 Transmembrane helix 13 from 288 (out) to 303 (in) : -962.069 Transmembrane helix 14 from 307 (in) to 322 (out) : -938.743 Transmembrane helix 15 from 331 (out) to 346 (in) : -755.353 Transmembrane helix 16 from 378 (in) to 393 (out) : -224.287 Transmembrane helix 17 from 397 (out) to 412 (in) : -213.008 Transmembrane helix 18 from 416 (in) to 431 (out) : -678.135 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 21 (in) to 36 (out) : -1043.25 Transmembrane helix 2 from 40 (out) to 55 (in) : -844.663 Transmembrane helix 3 from 59 (in) to 74 (out) : -830.15 Transmembrane helix 4 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 5 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 6 from 151 (out) to 166 (in) : -936.431 Transmembrane helix 7 from 172 (in) to 187 (out) : -858.911 Transmembrane helix 8 from 191 (out) to 206 (in) : -763.243 Transmembrane helix 9 from 214 (in) to 229 (out) : -904.335 Transmembrane helix 10 from 233 (out) to 248 (in) : -91.197 Transmembrane helix 11 from 252 (in) to 267 (out) : -622.147 Transmembrane helix 12 from 288 (out) to 303 (in) : -962.069 Transmembrane helix 13 from 307 (in) to 322 (out) : -938.743 Transmembrane helix 14 from 331 (out) to 346 (in) : -755.353 Transmembrane helix 15 from 378 (in) to 393 (out) : -224.287 Transmembrane helix 16 from 397 (out) to 412 (in) : -213.008 Transmembrane helix 17 from 416 (in) to 431 (out) : -678.135 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 38 (out) to 53 (in) : -859.234 Transmembrane helix 2 from 57 (in) to 72 (out) : -807.795 Transmembrane helix 3 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 4 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 5 from 151 (out) to 166 (in) : -936.431 Transmembrane helix 6 from 172 (in) to 187 (out) : -858.911 Transmembrane helix 7 from 191 (out) to 206 (in) : -763.243 Transmembrane helix 8 from 214 (in) to 229 (out) : -904.335 Transmembrane helix 9 from 233 (out) to 248 (in) : -91.197 Transmembrane helix 10 from 252 (in) to 267 (out) : -622.147 Transmembrane helix 11 from 288 (out) to 303 (in) : -962.069 Transmembrane helix 12 from 307 (in) to 322 (out) : -938.743 Transmembrane helix 13 from 331 (out) to 346 (in) : -755.353 Transmembrane helix 14 from 378 (in) to 393 (out) : -224.287 Transmembrane helix 15 from 397 (out) to 412 (in) : -213.008 Transmembrane helix 16 from 416 (in) to 431 (out) : -678.135 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 21 (in) to 36 (out) : -1043.25 Transmembrane helix 2 from 40 (out) to 55 (in) : -844.663 Transmembrane helix 3 from 59 (in) to 74 (out) : -830.15 Transmembrane helix 4 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 5 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 6 from 160 (out) to 175 (in) : -935.534 Transmembrane helix 7 from 183 (in) to 198 (out) : -670.359 Transmembrane helix 8 from 228 (out) to 243 (in) : -243.947 Transmembrane helix 9 from 247 (in) to 262 (out) : -378.793 Transmembrane helix 10 from 288 (out) to 303 (in) : -962.069 Transmembrane helix 11 from 307 (in) to 322 (out) : -938.743 Transmembrane helix 12 from 331 (out) to 346 (in) : -755.353 Transmembrane helix 13 from 378 (in) to 393 (out) : -224.287 Transmembrane helix 14 from 397 (out) to 412 (in) : -213.008 Transmembrane helix 15 from 416 (in) to 431 (out) : -678.135 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 38 (out) to 53 (in) : -859.234 Transmembrane helix 2 from 57 (in) to 72 (out) : -807.795 Transmembrane helix 3 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 4 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 5 from 160 (out) to 175 (in) : -935.534 Transmembrane helix 6 from 183 (in) to 198 (out) : -670.359 Transmembrane helix 7 from 228 (out) to 243 (in) : -243.947 Transmembrane helix 8 from 247 (in) to 262 (out) : -378.793 Transmembrane helix 9 from 288 (out) to 303 (in) : -962.069 Transmembrane helix 10 from 307 (in) to 322 (out) : -938.743 Transmembrane helix 11 from 331 (out) to 346 (in) : -755.353 Transmembrane helix 12 from 378 (in) to 393 (out) : -224.287 Transmembrane helix 13 from 397 (out) to 412 (in) : -213.008 Transmembrane helix 14 from 416 (in) to 431 (out) : -678.135 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 49 (in) to 64 (out) : -741.022 Transmembrane helix 2 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 3 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 4 from 160 (out) to 175 (in) : -935.534 Transmembrane helix 5 from 183 (in) to 198 (out) : -670.359 Transmembrane helix 6 from 228 (out) to 243 (in) : -243.947 Transmembrane helix 7 from 247 (in) to 262 (out) : -378.793 Transmembrane helix 8 from 288 (out) to 303 (in) : -962.069 Transmembrane helix 9 from 307 (in) to 322 (out) : -938.743 Transmembrane helix 10 from 331 (out) to 346 (in) : -755.353 Transmembrane helix 11 from 378 (in) to 393 (out) : -224.287 Transmembrane helix 12 from 397 (out) to 412 (in) : -213.008 Transmembrane helix 13 from 416 (in) to 431 (out) : -678.135 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 38 (out) to 53 (in) : -859.234 Transmembrane helix 2 from 57 (in) to 72 (out) : -807.795 Transmembrane helix 3 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 4 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 5 from 160 (out) to 175 (in) : -935.534 Transmembrane helix 6 from 183 (in) to 198 (out) : -670.359 Transmembrane helix 7 from 228 (out) to 243 (in) : -243.947 Transmembrane helix 8 from 247 (in) to 262 (out) : -378.793 Transmembrane helix 9 from 304 (out) to 319 (in) : -892.411 Transmembrane helix 10 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 11 from 382 (out) to 397 (in) : -23.305 Transmembrane helix 12 from 401 (in) to 416 (out) : -283.847 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 49 (in) to 64 (out) : -741.022 Transmembrane helix 2 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 3 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 4 from 160 (out) to 175 (in) : -935.534 Transmembrane helix 5 from 183 (in) to 198 (out) : -670.359 Transmembrane helix 6 from 228 (out) to 243 (in) : -243.947 Transmembrane helix 7 from 247 (in) to 262 (out) : -378.793 Transmembrane helix 8 from 304 (out) to 319 (in) : -892.411 Transmembrane helix 9 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 10 from 382 (out) to 397 (in) : -23.305 Transmembrane helix 11 from 401 (in) to 416 (out) : -283.847 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 38 (out) to 53 (in) : -859.234 Transmembrane helix 2 from 57 (in) to 72 (out) : -807.795 Transmembrane helix 3 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 4 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 5 from 183 (out) to 198 (in) : -675.941 Transmembrane helix 6 from 228 (in) to 243 (out) : -239.853 Transmembrane helix 7 from 247 (out) to 262 (in) : -388.107 Transmembrane helix 8 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 9 from 382 (out) to 397 (in) : -23.305 Transmembrane helix 10 from 401 (in) to 416 (out) : -283.847 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 49 (in) to 64 (out) : -741.022 Transmembrane helix 2 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 3 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 4 from 183 (out) to 198 (in) : -675.941 Transmembrane helix 5 from 228 (in) to 243 (out) : -239.853 Transmembrane helix 6 from 247 (out) to 262 (in) : -388.107 Transmembrane helix 7 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 8 from 382 (out) to 397 (in) : -23.305 Transmembrane helix 9 from 401 (in) to 416 (out) : -283.847 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 2 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 3 from 183 (out) to 198 (in) : -675.941 Transmembrane helix 4 from 228 (in) to 243 (out) : -239.853 Transmembrane helix 5 from 247 (out) to 262 (in) : -388.107 Transmembrane helix 6 from 331 (in) to 346 (out) : -755.847 Transmembrane helix 7 from 382 (out) to 397 (in) : -23.305 Transmembrane helix 8 from 401 (in) to 416 (out) : -283.847 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 99 (in) to 114 (out) : -372.334 Transmembrane helix 2 from 118 (out) to 133 (in) : -30.769 Transmembrane helix 3 from 183 (in) to 198 (out) : -670.359 Transmembrane helix 4 from 228 (out) to 243 (in) : -243.947 Transmembrane helix 5 from 247 (in) to 262 (out) : -378.793 Transmembrane helix 6 from 382 (out) to 397 (in) : -23.305 Transmembrane helix 7 from 401 (in) to 416 (out) : -283.847 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 99 (out) to 114 (in) : -371.666 Transmembrane helix 2 from 118 (in) to 133 (out) : -8.631 Transmembrane helix 3 from 228 (out) to 243 (in) : -243.947 Transmembrane helix 4 from 247 (in) to 262 (out) : -378.793 Transmembrane helix 5 from 382 (out) to 397 (in) : -23.305 Transmembrane helix 6 from 401 (in) to 416 (out) : -283.847 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 99 (in) to 114 (out) : -372.334 Transmembrane helix 2 from 118 (out) to 133 (in) : -30.769 Transmembrane helix 3 from 236 (in) to 251 (out) : -28.597 Transmembrane helix 4 from 382 (out) to 397 (in) : -23.305 Transmembrane helix 5 from 401 (in) to 416 (out) : -283.847 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 111 (out) to 126 (in) : 122.455 Transmembrane helix 2 from 236 (in) to 251 (out) : -28.597 Transmembrane helix 3 from 382 (out) to 397 (in) : -23.305 Transmembrane helix 4 from 401 (in) to 416 (out) : -283.847 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 111 (in) to 126 (out) : 138.445 Transmembrane helix 2 from 236 (out) to 251 (in) : -31.003 Transmembrane helix 3 from 387 (in) to 402 (out) : 23.526 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 111 (out) to 126 (in) : 122.455 Transmembrane helix 2 from 387 (in) to 402 (out) : 23.526 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.138445 1 helix (-) : Score = 0.122455 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 22 helices (+) : Score = -100000 22 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 46.092 Topology: 111-126 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.138445