BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (418 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P36661 Uncharacterized protein yccE n=37 Tax=Enterobact...   860   0.0  
UniRef50_B1ERU3 Putative uncharacterized protein n=1 Tax=Escheri...    48   7e-04

>UniRef50_P36661 Uncharacterized protein yccE n=37 Tax=Enterobacteriaceae
           RepID=YCCE_ECOLI
          Length = 418

 Score =  860 bits (2223), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 418/418 (100%), Positives = 418/418 (100%)

Query: 1   MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVCFMRLYNELRRKVNEED 60
           MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVCFMRLYNELRRKVNEED
Sbjct: 1   MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVCFMRLYNELRRKVNEED 60

Query: 61  TPCLECESLEKEFEEMQNDNDLSLFMRILRTNDTQIYSGVSGGITYTIQYVRDIDIVRVS 120
           TPCLECESLEKEFEEMQNDNDLSLFMRILRTNDTQIYSGVSGGITYTIQYVRDIDIVRVS
Sbjct: 61  TPCLECESLEKEFEEMQNDNDLSLFMRILRTNDTQIYSGVSGGITYTIQYVRDIDIVRVS 120

Query: 121 LPGRASESITDFKGYYWYNFMEYIENINACDDVFSEYCFDDENISVQPERINTPGISDLD 180
           LPGRASESITDFKGYYWYNFMEYIENINACDDVFSEYCFDDENISVQPERINTPGISDLD
Sbjct: 121 LPGRASESITDFKGYYWYNFMEYIENINACDDVFSEYCFDDENISVQPERINTPGISDLD 180

Query: 181 SDIDLSGISFIQRETNQALGLKYAPVDGDGYCLLRAILVLKQHDYSWALVSYKMQKEVYN 240
           SDIDLSGISFIQRETNQALGLKYAPVDGDGYCLLRAILVLKQHDYSWALVSYKMQKEVYN
Sbjct: 181 SDIDLSGISFIQRETNQALGLKYAPVDGDGYCLLRAILVLKQHDYSWALVSYKMQKEVYN 240

Query: 241 EFIKMVDKKTIEALVDTAFYNLREDVKTLFGVDLQSDNQIQGQSSLMSWSFLFFKKQFID 300
           EFIKMVDKKTIEALVDTAFYNLREDVKTLFGVDLQSDNQIQGQSSLMSWSFLFFKKQFID
Sbjct: 241 EFIKMVDKKTIEALVDTAFYNLREDVKTLFGVDLQSDNQIQGQSSLMSWSFLFFKKQFID 300

Query: 301 SCLNNEKCILHLPEFIFNDNKNLLALDTDTSDRIKAVKNFLVVLSDSICSLFIVNSNVAS 360
           SCLNNEKCILHLPEFIFNDNKNLLALDTDTSDRIKAVKNFLVVLSDSICSLFIVNSNVAS
Sbjct: 301 SCLNNEKCILHLPEFIFNDNKNLLALDTDTSDRIKAVKNFLVVLSDSICSLFIVNSNVAS 360

Query: 361 ISLGNESFSTDEDLEYGYLMNTGNHYDVYLPPELFAQAYKLNNKEMNAQLDYLNRYAI 418
           ISLGNESFSTDEDLEYGYLMNTGNHYDVYLPPELFAQAYKLNNKEMNAQLDYLNRYAI
Sbjct: 361 ISLGNESFSTDEDLEYGYLMNTGNHYDVYLPPELFAQAYKLNNKEMNAQLDYLNRYAI 418


>UniRef50_B1ERU3 Putative uncharacterized protein n=1 Tax=Escherichia albertii
           TW07627 RepID=B1ERU3_9ESCH
          Length = 175

 Score = 47.8 bits (112), Expect = 7e-04,   Method: Compositional matrix adjust.
 Identities = 44/193 (22%), Positives = 86/193 (44%), Gaps = 37/193 (19%)

Query: 213 LLRAILVLKQHDYSWALVSYKMQKEVYNEFIKMVDKKTIEALVDTAFYNLREDVKTLFGV 272
           + RAIL +   D SWA  + K   EVY  F                      D+  L  +
Sbjct: 1   MFRAILAILDKDSSWADKNVKTSSEVYGSF--------------------ASDIANLNQI 40

Query: 273 DLQSDNQIQGQSSLMSWSFLF--FKKQFIDSCLNNEKCILHLPEFIFND-NKNLLALDTD 329
            +    +++G    +S   L   F++  +  C  + +C+++ P  IFN  + + +++D +
Sbjct: 41  AVNVCEELEGMEFFLSDYPLLEDFEENVVKKCFRDNECVIYSPTGIFNACSVSEMSIDDN 100

Query: 330 TSDRIKAVKNFLVVLSDSICSLFIVNSNVASISLGNES--FSTDED----LEYGYLMNT- 382
            SD       +L + + S  +  + +  +    + ++   F  D+D    ++ GY++N  
Sbjct: 101 YSD-------YLSIWASSFAAKLLEHYGIVIKKIADDGTEFWEDQDKLNHIDNGYVLNVN 153

Query: 383 GNHYDVYLPPELF 395
           GNHY+V LP ++F
Sbjct: 154 GNHYNVRLPLDIF 166


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P36661 Uncharacterized protein yccE n=37 Tax=Enterobact...   764   0.0  
UniRef50_B1ERU3 Putative uncharacterized protein n=1 Tax=Escheri...   212   2e-53

Sequences not found previously or not previously below threshold:

UniRef50_UPI0001850E26 hypothetical protein Bcoam_11275 n=1 Tax=...    41   0.098


CONVERGED!
>UniRef50_P36661 Uncharacterized protein yccE n=37 Tax=Enterobacteriaceae
           RepID=YCCE_ECOLI
          Length = 418

 Score =  764 bits (1973), Expect = 0.0,   Method: Composition-based stats.
 Identities = 418/418 (100%), Positives = 418/418 (100%)

Query: 1   MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVCFMRLYNELRRKVNEED 60
           MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVCFMRLYNELRRKVNEED
Sbjct: 1   MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVCFMRLYNELRRKVNEED 60

Query: 61  TPCLECESLEKEFEEMQNDNDLSLFMRILRTNDTQIYSGVSGGITYTIQYVRDIDIVRVS 120
           TPCLECESLEKEFEEMQNDNDLSLFMRILRTNDTQIYSGVSGGITYTIQYVRDIDIVRVS
Sbjct: 61  TPCLECESLEKEFEEMQNDNDLSLFMRILRTNDTQIYSGVSGGITYTIQYVRDIDIVRVS 120

Query: 121 LPGRASESITDFKGYYWYNFMEYIENINACDDVFSEYCFDDENISVQPERINTPGISDLD 180
           LPGRASESITDFKGYYWYNFMEYIENINACDDVFSEYCFDDENISVQPERINTPGISDLD
Sbjct: 121 LPGRASESITDFKGYYWYNFMEYIENINACDDVFSEYCFDDENISVQPERINTPGISDLD 180

Query: 181 SDIDLSGISFIQRETNQALGLKYAPVDGDGYCLLRAILVLKQHDYSWALVSYKMQKEVYN 240
           SDIDLSGISFIQRETNQALGLKYAPVDGDGYCLLRAILVLKQHDYSWALVSYKMQKEVYN
Sbjct: 181 SDIDLSGISFIQRETNQALGLKYAPVDGDGYCLLRAILVLKQHDYSWALVSYKMQKEVYN 240

Query: 241 EFIKMVDKKTIEALVDTAFYNLREDVKTLFGVDLQSDNQIQGQSSLMSWSFLFFKKQFID 300
           EFIKMVDKKTIEALVDTAFYNLREDVKTLFGVDLQSDNQIQGQSSLMSWSFLFFKKQFID
Sbjct: 241 EFIKMVDKKTIEALVDTAFYNLREDVKTLFGVDLQSDNQIQGQSSLMSWSFLFFKKQFID 300

Query: 301 SCLNNEKCILHLPEFIFNDNKNLLALDTDTSDRIKAVKNFLVVLSDSICSLFIVNSNVAS 360
           SCLNNEKCILHLPEFIFNDNKNLLALDTDTSDRIKAVKNFLVVLSDSICSLFIVNSNVAS
Sbjct: 301 SCLNNEKCILHLPEFIFNDNKNLLALDTDTSDRIKAVKNFLVVLSDSICSLFIVNSNVAS 360

Query: 361 ISLGNESFSTDEDLEYGYLMNTGNHYDVYLPPELFAQAYKLNNKEMNAQLDYLNRYAI 418
           ISLGNESFSTDEDLEYGYLMNTGNHYDVYLPPELFAQAYKLNNKEMNAQLDYLNRYAI
Sbjct: 361 ISLGNESFSTDEDLEYGYLMNTGNHYDVYLPPELFAQAYKLNNKEMNAQLDYLNRYAI 418


>UniRef50_B1ERU3 Putative uncharacterized protein n=1 Tax=Escherichia albertii
           TW07627 RepID=B1ERU3_9ESCH
          Length = 175

 Score =  212 bits (540), Expect = 2e-53,   Method: Composition-based stats.
 Identities = 44/193 (22%), Positives = 86/193 (44%), Gaps = 37/193 (19%)

Query: 213 LLRAILVLKQHDYSWALVSYKMQKEVYNEFIKMVDKKTIEALVDTAFYNLREDVKTLFGV 272
           + RAIL +   D SWA  + K   EVY  F                      D+  L  +
Sbjct: 1   MFRAILAILDKDSSWADKNVKTSSEVYGSF--------------------ASDIANLNQI 40

Query: 273 DLQSDNQIQGQSSLMSWSFLF--FKKQFIDSCLNNEKCILHLPEFIFND-NKNLLALDTD 329
            +    +++G    +S   L   F++  +  C  + +C+++ P  IFN  + + +++D +
Sbjct: 41  AVNVCEELEGMEFFLSDYPLLEDFEENVVKKCFRDNECVIYSPTGIFNACSVSEMSIDDN 100

Query: 330 TSDRIKAVKNFLVVLSDSICSLFIVNSNVASISLGNES--FSTDED----LEYGYLMNT- 382
            SD       +L + + S  +  + +  +    + ++   F  D+D    ++ GY++N  
Sbjct: 101 YSD-------YLSIWASSFAAKLLEHYGIVIKKIADDGTEFWEDQDKLNHIDNGYVLNVN 153

Query: 383 GNHYDVYLPPELF 395
           GNHY+V LP ++F
Sbjct: 154 GNHYNVRLPLDIF 166


>UniRef50_UPI0001850E26 hypothetical protein Bcoam_11275 n=1 Tax=Bacillus coahuilensis m4-4
           RepID=UPI0001850E26
          Length = 355

 Score = 40.8 bits (94), Expect = 0.098,   Method: Composition-based stats.
 Identities = 33/143 (23%), Positives = 58/143 (40%), Gaps = 5/143 (3%)

Query: 233 KMQKEVYNEFIKMVDKKTIEALVDTAFYNLREDVKTLFGVDLQSDNQIQGQSSLMSWSFL 292
           K+  E+Y E I +++K+  E  VD +   +R     + G   Q  +Q+ G  S+M W   
Sbjct: 168 KLTGELYKEIIDILEKE--EFPVDPSIITIRLSGYQVAGKTTQQSSQLLGMDSVMYWYRF 225

Query: 293 FFKKQFIDSCLNNEKCILHLPEFIFNDNKNLLALDTDTSDRIKAVKNFLVVLSDSICSLF 352
                +I   + + +       F+  D  +   L   T+   + VK  L +   S     
Sbjct: 226 LEGITYIVKLIKDNQREFPHLSFLVADLMSDFTLTESTTKTYELVKRGLSLKEISSARKL 285

Query: 353 ---IVNSNVASISLGNESFSTDE 372
               +  +V  I+L + SFS D 
Sbjct: 286 RLSTIEDHVIEIALNDSSFSIDH 308


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.318    0.132    0.375 

Lambda     K      H
   0.267   0.0405    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,658,254,956
Number of Sequences: 3077464
Number of extensions: 70162186
Number of successful extensions: 193840
Number of sequences better than 1.0e-01: 3
Number of HSP's better than  0.1 without gapping: 3
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 193828
Number of HSP's gapped (non-prelim): 7
length of query: 418
length of database: 1,040,396,356
effective HSP length: 131
effective length of query: 287
effective length of database: 637,248,572
effective search space: 182890340164
effective search space used: 182890340164
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 94 (40.8 bits)
