Processing 1 helix: Transmembrane helix 1 from 99 (in) to 122 (out) : 2735.05 Score = 2.731943 Processing 1 helix: Transmembrane helix 1 from 99 (out) to 122 (in) : 2743.75 Score = 2.746856 Processing 6 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 440.281 Transmembrane helix 2 from 23 (out) to 38 (in) : -5.24 Transmembrane helix 3 from 42 (in) to 57 (out) : 1138.45 Transmembrane helix 4 from 62 (out) to 77 (in) : 154.306 Transmembrane helix 5 from 81 (in) to 96 (out) : -162.174 Transmembrane helix 6 from 100 (out) to 122 (in) : 2732.87 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 5 (out) to 22 (in) : 535.738 Transmembrane helix 2 from 35 (in) to 54 (out) : 1486.12 Transmembrane helix 3 from 62 (out) to 77 (in) : 154.306 Transmembrane helix 4 from 81 (in) to 96 (out) : -162.174 Transmembrane helix 5 from 100 (out) to 122 (in) : 2732.87 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 6 (in) to 22 (out) : 511.711 Transmembrane helix 2 from 35 (out) to 54 (in) : 1500.68 Transmembrane helix 3 from 68 (in) to 83 (out) : 338.82 Transmembrane helix 4 from 99 (out) to 122 (in) : 2743.75 Score = 5.090592 Processing 3 helices: Transmembrane helix 1 from 5 (out) to 22 (in) : 535.738 Transmembrane helix 2 from 35 (in) to 54 (out) : 1486.12 Transmembrane helix 3 from 99 (out) to 122 (in) : 2743.75 Score = 4.776056 Processing 2 helices: Transmembrane helix 1 from 35 (in) to 54 (out) : 1486.12 Transmembrane helix 2 from 99 (out) to 122 (in) : 2743.75 Score = 4.252409 Processing 6 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 463.419 Transmembrane helix 2 from 23 (in) to 38 (out) : 2.14 Transmembrane helix 3 from 42 (out) to 57 (in) : 1109.56 Transmembrane helix 4 from 62 (in) to 77 (out) : 133.594 Transmembrane helix 5 from 81 (out) to 96 (in) : -176.926 Transmembrane helix 6 from 100 (in) to 122 (out) : 2726.13 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 6 (in) to 22 (out) : 511.711 Transmembrane helix 2 from 35 (out) to 54 (in) : 1500.68 Transmembrane helix 3 from 62 (in) to 77 (out) : 133.594 Transmembrane helix 4 from 81 (out) to 96 (in) : -176.926 Transmembrane helix 5 from 100 (in) to 122 (out) : 2726.13 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 5 (out) to 22 (in) : 535.738 Transmembrane helix 2 from 35 (in) to 54 (out) : 1486.12 Transmembrane helix 3 from 68 (out) to 83 (in) : 355.48 Transmembrane helix 4 from 99 (in) to 122 (out) : 2735.05 Score = 5.116509 Processing 3 helices: Transmembrane helix 1 from 6 (in) to 22 (out) : 511.711 Transmembrane helix 2 from 35 (out) to 54 (in) : 1500.68 Transmembrane helix 3 from 99 (in) to 122 (out) : 2735.05 Score = 4.736744 Processing 2 helices: Transmembrane helix 1 from 35 (out) to 54 (in) : 1500.68 Transmembrane helix 2 from 99 (in) to 122 (out) : 2735.05 Score = 4.213191 Summary of topology analysis: 1 helix (+) : Score = 2.73194 1 helix (-) : Score = 2.74686 2 helices (+) : Score = 4.25241 2 helices (-) : Score = 4.21319 3 helices (+) : Score = 4.73674 3 helices (-) : Score = 4.77606 4 helices (+) : Score = 5.09059 4 helices (-) : Score = 5.11651 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 5-22,35-54,68-83,99-122 Re-entrant helices: Not detected. Helix count: 4 N-terminal: out Score: 5.11651