Processing 1 helix: Transmembrane helix 1 from 18 (in) to 35 (out) : 3410.21 Score = 3.450911 Processing 1 helix: Transmembrane helix 1 from 18 (out) to 35 (in) : 3381.19 Score = 3.340489 Processing 28 helices: Transmembrane helix 1 from 5 (in) to 20 (out) : -290.608 Transmembrane helix 2 from 24 (out) to 39 (in) : 2430.56 Transmembrane helix 3 from 43 (in) to 58 (out) : -663.017 Transmembrane helix 4 from 62 (out) to 77 (in) : -701.076 Transmembrane helix 5 from 81 (in) to 96 (out) : -1131.47 Transmembrane helix 6 from 100 (out) to 115 (in) : -1071.61 Transmembrane helix 7 from 119 (in) to 134 (out) : -1061.1 Transmembrane helix 8 from 139 (out) to 154 (in) : -1402.98 Transmembrane helix 9 from 158 (in) to 173 (out) : -902.279 Transmembrane helix 10 from 177 (out) to 192 (in) : -781.425 Transmembrane helix 11 from 196 (in) to 211 (out) : -935.74 Transmembrane helix 12 from 215 (out) to 230 (in) : -631.068 Transmembrane helix 13 from 234 (in) to 249 (out) : -333.987 Transmembrane helix 14 from 253 (out) to 268 (in) : -1397.18 Transmembrane helix 15 from 272 (in) to 287 (out) : -1064.85 Transmembrane helix 16 from 291 (out) to 306 (in) : -529.906 Transmembrane helix 17 from 310 (in) to 325 (out) : -710.668 Transmembrane helix 18 from 329 (out) to 344 (in) : -866.325 Transmembrane helix 19 from 348 (in) to 363 (out) : -1095.1 Transmembrane helix 20 from 367 (out) to 382 (in) : -867.714 Transmembrane helix 21 from 386 (in) to 401 (out) : -1434.73 Transmembrane helix 22 from 405 (out) to 420 (in) : -864.251 Transmembrane helix 23 from 424 (in) to 439 (out) : -1591.37 Transmembrane helix 24 from 443 (out) to 458 (in) : -1386.01 Transmembrane helix 25 from 468 (in) to 483 (out) : -1712.41 Transmembrane helix 26 from 487 (out) to 502 (in) : -1392.29 Transmembrane helix 27 from 506 (in) to 521 (out) : -1419.51 Transmembrane helix 28 from 527 (out) to 542 (in) : -1488.94 Score = -100000.000000 Processing 27 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -564.823 Transmembrane helix 2 from 23 (in) to 38 (out) : 2693.13 Transmembrane helix 3 from 42 (out) to 57 (in) : -684.643 Transmembrane helix 4 from 61 (in) to 76 (out) : -700.633 Transmembrane helix 5 from 80 (out) to 95 (in) : -1115.11 Transmembrane helix 6 from 99 (in) to 114 (out) : -1101.82 Transmembrane helix 7 from 118 (out) to 133 (in) : -1004.39 Transmembrane helix 8 from 144 (in) to 159 (out) : -1257.21 Transmembrane helix 9 from 163 (out) to 178 (in) : -853.822 Transmembrane helix 10 from 182 (in) to 197 (out) : -774.267 Transmembrane helix 11 from 201 (out) to 216 (in) : -971.539 Transmembrane helix 12 from 220 (in) to 235 (out) : -568.134 Transmembrane helix 13 from 239 (out) to 254 (in) : -426.434 Transmembrane helix 14 from 259 (in) to 274 (out) : -1415.74 Transmembrane helix 15 from 278 (out) to 293 (in) : -879.506 Transmembrane helix 16 from 297 (in) to 312 (out) : -467.457 Transmembrane helix 17 from 316 (out) to 331 (in) : -886.348 Transmembrane helix 18 from 335 (in) to 350 (out) : -797.065 Transmembrane helix 19 from 354 (out) to 369 (in) : -1142.07 Transmembrane helix 20 from 373 (in) to 388 (out) : -966.301 Transmembrane helix 21 from 395 (out) to 410 (in) : -1207.03 Transmembrane helix 22 from 414 (in) to 429 (out) : -1032.68 Transmembrane helix 23 from 441 (out) to 456 (in) : -1385.84 Transmembrane helix 24 from 468 (in) to 483 (out) : -1712.41 Transmembrane helix 25 from 487 (out) to 502 (in) : -1392.29 Transmembrane helix 26 from 506 (in) to 521 (out) : -1419.51 Transmembrane helix 27 from 527 (out) to 542 (in) : -1488.94 Score = -100000.000000 Processing 26 helices: Transmembrane helix 1 from 5 (in) to 20 (out) : -290.608 Transmembrane helix 2 from 24 (out) to 39 (in) : 2430.56 Transmembrane helix 3 from 43 (in) to 58 (out) : -663.017 Transmembrane helix 4 from 62 (out) to 77 (in) : -701.076 Transmembrane helix 5 from 81 (in) to 96 (out) : -1131.47 Transmembrane helix 6 from 100 (out) to 115 (in) : -1071.61 Transmembrane helix 7 from 119 (in) to 134 (out) : -1061.1 Transmembrane helix 8 from 144 (out) to 159 (in) : -1260.19 Transmembrane helix 9 from 163 (in) to 178 (out) : -848.778 Transmembrane helix 10 from 182 (out) to 197 (in) : -774.033 Transmembrane helix 11 from 201 (in) to 216 (out) : -973.761 Transmembrane helix 12 from 220 (out) to 235 (in) : -572.866 Transmembrane helix 13 from 239 (in) to 254 (out) : -430.566 Transmembrane helix 14 from 259 (out) to 274 (in) : -1419.86 Transmembrane helix 15 from 278 (in) to 293 (out) : -885.494 Transmembrane helix 16 from 297 (out) to 312 (in) : -464.943 Transmembrane helix 17 from 316 (in) to 331 (out) : -898.652 Transmembrane helix 18 from 335 (out) to 350 (in) : -793.735 Transmembrane helix 19 from 354 (in) to 369 (out) : -1142.33 Transmembrane helix 20 from 373 (out) to 388 (in) : -975.499 Transmembrane helix 21 from 394 (in) to 409 (out) : -1250.32 Transmembrane helix 22 from 413 (out) to 428 (in) : -975.292 Transmembrane helix 23 from 441 (in) to 456 (out) : -1387.66 Transmembrane helix 24 from 482 (out) to 497 (in) : -1354.56 Transmembrane helix 25 from 505 (in) to 520 (out) : -1418.5 Transmembrane helix 26 from 527 (out) to 542 (in) : -1488.94 Score = -100000.000000 Processing 25 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -564.823 Transmembrane helix 2 from 23 (in) to 38 (out) : 2693.13 Transmembrane helix 3 from 42 (out) to 57 (in) : -684.643 Transmembrane helix 4 from 61 (in) to 76 (out) : -700.633 Transmembrane helix 5 from 80 (out) to 95 (in) : -1115.11 Transmembrane helix 6 from 99 (in) to 114 (out) : -1101.82 Transmembrane helix 7 from 118 (out) to 133 (in) : -1004.39 Transmembrane helix 8 from 144 (in) to 159 (out) : -1257.21 Transmembrane helix 9 from 163 (out) to 178 (in) : -853.822 Transmembrane helix 10 from 182 (in) to 197 (out) : -774.267 Transmembrane helix 11 from 201 (out) to 216 (in) : -971.539 Transmembrane helix 12 from 220 (in) to 235 (out) : -568.134 Transmembrane helix 13 from 239 (out) to 254 (in) : -426.434 Transmembrane helix 14 from 259 (in) to 274 (out) : -1415.74 Transmembrane helix 15 from 278 (out) to 293 (in) : -879.506 Transmembrane helix 16 from 297 (in) to 312 (out) : -467.457 Transmembrane helix 17 from 316 (out) to 331 (in) : -886.348 Transmembrane helix 18 from 335 (in) to 350 (out) : -797.065 Transmembrane helix 19 from 354 (out) to 369 (in) : -1142.07 Transmembrane helix 20 from 373 (in) to 388 (out) : -966.301 Transmembrane helix 21 from 395 (out) to 410 (in) : -1207.03 Transmembrane helix 22 from 414 (in) to 429 (out) : -1032.68 Transmembrane helix 23 from 441 (out) to 456 (in) : -1385.84 Transmembrane helix 24 from 482 (in) to 497 (out) : -1355.94 Transmembrane helix 25 from 506 (out) to 521 (in) : -1417.09 Score = -100000.000000 Processing 24 helices: Transmembrane helix 1 from 18 (in) to 34 (out) : 3375.86 Transmembrane helix 2 from 38 (out) to 53 (in) : -672.426 Transmembrane helix 3 from 57 (in) to 72 (out) : -663.182 Transmembrane helix 4 from 76 (out) to 91 (in) : -1039.15 Transmembrane helix 5 from 95 (in) to 110 (out) : -1169.59 Transmembrane helix 6 from 114 (out) to 129 (in) : -796.663 Transmembrane helix 7 from 144 (in) to 159 (out) : -1257.21 Transmembrane helix 8 from 163 (out) to 178 (in) : -853.822 Transmembrane helix 9 from 182 (in) to 197 (out) : -774.267 Transmembrane helix 10 from 201 (out) to 216 (in) : -971.539 Transmembrane helix 11 from 220 (in) to 235 (out) : -568.134 Transmembrane helix 12 from 239 (out) to 254 (in) : -426.434 Transmembrane helix 13 from 259 (in) to 274 (out) : -1415.74 Transmembrane helix 14 from 278 (out) to 293 (in) : -879.506 Transmembrane helix 15 from 297 (in) to 312 (out) : -467.457 Transmembrane helix 16 from 316 (out) to 331 (in) : -886.348 Transmembrane helix 17 from 335 (in) to 350 (out) : -797.065 Transmembrane helix 18 from 354 (out) to 369 (in) : -1142.07 Transmembrane helix 19 from 373 (in) to 388 (out) : -966.301 Transmembrane helix 20 from 395 (out) to 410 (in) : -1207.03 Transmembrane helix 21 from 414 (in) to 429 (out) : -1032.68 Transmembrane helix 22 from 441 (out) to 456 (in) : -1385.84 Transmembrane helix 23 from 482 (in) to 497 (out) : -1355.94 Transmembrane helix 24 from 506 (out) to 521 (in) : -1417.09 Score = -100000.000000 Processing 23 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : 3256.78 Transmembrane helix 2 from 37 (in) to 52 (out) : -633.542 Transmembrane helix 3 from 56 (out) to 71 (in) : -644.712 Transmembrane helix 4 from 75 (in) to 90 (out) : -1013.83 Transmembrane helix 5 from 94 (out) to 109 (in) : -1179.32 Transmembrane helix 6 from 113 (in) to 128 (out) : -763.605 Transmembrane helix 7 from 144 (out) to 159 (in) : -1260.19 Transmembrane helix 8 from 163 (in) to 178 (out) : -848.778 Transmembrane helix 9 from 182 (out) to 197 (in) : -774.033 Transmembrane helix 10 from 201 (in) to 216 (out) : -973.761 Transmembrane helix 11 from 220 (out) to 235 (in) : -572.866 Transmembrane helix 12 from 239 (in) to 254 (out) : -430.566 Transmembrane helix 13 from 273 (out) to 288 (in) : -1031.13 Transmembrane helix 14 from 292 (in) to 307 (out) : -508.813 Transmembrane helix 15 from 311 (out) to 326 (in) : -743.785 Transmembrane helix 16 from 330 (in) to 345 (out) : -848.518 Transmembrane helix 17 from 349 (out) to 364 (in) : -1104.35 Transmembrane helix 18 from 368 (in) to 383 (out) : -856.017 Transmembrane helix 19 from 395 (out) to 410 (in) : -1207.03 Transmembrane helix 20 from 414 (in) to 429 (out) : -1032.68 Transmembrane helix 21 from 441 (out) to 456 (in) : -1385.84 Transmembrane helix 22 from 482 (in) to 497 (out) : -1355.94 Transmembrane helix 23 from 506 (out) to 521 (in) : -1417.09 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 18 (in) to 34 (out) : 3375.86 Transmembrane helix 2 from 38 (out) to 53 (in) : -672.426 Transmembrane helix 3 from 57 (in) to 72 (out) : -663.182 Transmembrane helix 4 from 76 (out) to 91 (in) : -1039.15 Transmembrane helix 5 from 95 (in) to 110 (out) : -1169.59 Transmembrane helix 6 from 114 (out) to 129 (in) : -796.663 Transmembrane helix 7 from 144 (in) to 159 (out) : -1257.21 Transmembrane helix 8 from 163 (out) to 178 (in) : -853.822 Transmembrane helix 9 from 182 (in) to 197 (out) : -774.267 Transmembrane helix 10 from 201 (out) to 216 (in) : -971.539 Transmembrane helix 11 from 220 (in) to 235 (out) : -568.134 Transmembrane helix 12 from 239 (out) to 254 (in) : -426.434 Transmembrane helix 13 from 268 (in) to 283 (out) : -1149.99 Transmembrane helix 14 from 287 (out) to 302 (in) : -610.602 Transmembrane helix 15 from 306 (in) to 321 (out) : -584.107 Transmembrane helix 16 from 325 (out) to 340 (in) : -914.109 Transmembrane helix 17 from 344 (in) to 359 (out) : -991.601 Transmembrane helix 18 from 368 (out) to 383 (in) : -863.983 Transmembrane helix 19 from 394 (in) to 409 (out) : -1250.32 Transmembrane helix 20 from 413 (out) to 428 (in) : -975.292 Transmembrane helix 21 from 441 (in) to 456 (out) : -1387.66 Transmembrane helix 22 from 482 (out) to 497 (in) : -1354.56 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : 3256.78 Transmembrane helix 2 from 37 (in) to 52 (out) : -633.542 Transmembrane helix 3 from 56 (out) to 71 (in) : -644.712 Transmembrane helix 4 from 75 (in) to 90 (out) : -1013.83 Transmembrane helix 5 from 94 (out) to 109 (in) : -1179.32 Transmembrane helix 6 from 113 (in) to 128 (out) : -763.605 Transmembrane helix 7 from 144 (out) to 159 (in) : -1260.19 Transmembrane helix 8 from 163 (in) to 178 (out) : -848.778 Transmembrane helix 9 from 182 (out) to 197 (in) : -774.033 Transmembrane helix 10 from 201 (in) to 216 (out) : -973.761 Transmembrane helix 11 from 220 (out) to 235 (in) : -572.866 Transmembrane helix 12 from 239 (in) to 254 (out) : -430.566 Transmembrane helix 13 from 273 (out) to 288 (in) : -1031.13 Transmembrane helix 14 from 292 (in) to 307 (out) : -508.813 Transmembrane helix 15 from 311 (out) to 326 (in) : -743.785 Transmembrane helix 16 from 330 (in) to 345 (out) : -848.518 Transmembrane helix 17 from 349 (out) to 364 (in) : -1104.35 Transmembrane helix 18 from 368 (in) to 383 (out) : -856.017 Transmembrane helix 19 from 406 (out) to 421 (in) : -852.082 Transmembrane helix 20 from 441 (in) to 456 (out) : -1387.66 Transmembrane helix 21 from 482 (out) to 497 (in) : -1354.56 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 18 (in) to 34 (out) : 3375.86 Transmembrane helix 2 from 38 (out) to 53 (in) : -672.426 Transmembrane helix 3 from 57 (in) to 72 (out) : -663.182 Transmembrane helix 4 from 76 (out) to 91 (in) : -1039.15 Transmembrane helix 5 from 95 (in) to 110 (out) : -1169.59 Transmembrane helix 6 from 114 (out) to 129 (in) : -796.663 Transmembrane helix 7 from 144 (in) to 159 (out) : -1257.21 Transmembrane helix 8 from 163 (out) to 178 (in) : -853.822 Transmembrane helix 9 from 182 (in) to 197 (out) : -774.267 Transmembrane helix 10 from 201 (out) to 216 (in) : -971.539 Transmembrane helix 11 from 220 (in) to 235 (out) : -568.134 Transmembrane helix 12 from 239 (out) to 254 (in) : -426.434 Transmembrane helix 13 from 268 (in) to 283 (out) : -1149.99 Transmembrane helix 14 from 287 (out) to 302 (in) : -610.602 Transmembrane helix 15 from 306 (in) to 321 (out) : -584.107 Transmembrane helix 16 from 325 (out) to 340 (in) : -914.109 Transmembrane helix 17 from 344 (in) to 359 (out) : -991.601 Transmembrane helix 18 from 368 (out) to 383 (in) : -863.983 Transmembrane helix 19 from 395 (in) to 410 (out) : -1210.97 Transmembrane helix 20 from 414 (out) to 429 (in) : -1012.92 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : 3256.78 Transmembrane helix 2 from 37 (in) to 52 (out) : -633.542 Transmembrane helix 3 from 56 (out) to 71 (in) : -644.712 Transmembrane helix 4 from 75 (in) to 90 (out) : -1013.83 Transmembrane helix 5 from 94 (out) to 109 (in) : -1179.32 Transmembrane helix 6 from 113 (in) to 128 (out) : -763.605 Transmembrane helix 7 from 144 (out) to 159 (in) : -1260.19 Transmembrane helix 8 from 163 (in) to 178 (out) : -848.778 Transmembrane helix 9 from 182 (out) to 197 (in) : -774.033 Transmembrane helix 10 from 201 (in) to 216 (out) : -973.761 Transmembrane helix 11 from 220 (out) to 235 (in) : -572.866 Transmembrane helix 12 from 239 (in) to 254 (out) : -430.566 Transmembrane helix 13 from 273 (out) to 288 (in) : -1031.13 Transmembrane helix 14 from 292 (in) to 307 (out) : -508.813 Transmembrane helix 15 from 311 (out) to 326 (in) : -743.785 Transmembrane helix 16 from 330 (in) to 345 (out) : -848.518 Transmembrane helix 17 from 349 (out) to 364 (in) : -1104.35 Transmembrane helix 18 from 368 (in) to 383 (out) : -856.017 Transmembrane helix 19 from 406 (out) to 421 (in) : -852.082 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 18 (in) to 34 (out) : 3375.86 Transmembrane helix 2 from 38 (out) to 53 (in) : -672.426 Transmembrane helix 3 from 57 (in) to 72 (out) : -663.182 Transmembrane helix 4 from 76 (out) to 91 (in) : -1039.15 Transmembrane helix 5 from 95 (in) to 110 (out) : -1169.59 Transmembrane helix 6 from 114 (out) to 129 (in) : -796.663 Transmembrane helix 7 from 159 (in) to 174 (out) : -892.228 Transmembrane helix 8 from 178 (out) to 193 (in) : -778.129 Transmembrane helix 9 from 197 (in) to 212 (out) : -945.531 Transmembrane helix 10 from 218 (out) to 233 (in) : -586.362 Transmembrane helix 11 from 237 (in) to 252 (out) : -361.304 Transmembrane helix 12 from 273 (out) to 288 (in) : -1031.13 Transmembrane helix 13 from 292 (in) to 307 (out) : -508.813 Transmembrane helix 14 from 311 (out) to 326 (in) : -743.785 Transmembrane helix 15 from 330 (in) to 345 (out) : -848.518 Transmembrane helix 16 from 349 (out) to 364 (in) : -1104.35 Transmembrane helix 17 from 368 (in) to 383 (out) : -856.017 Transmembrane helix 18 from 406 (out) to 421 (in) : -852.082 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : 3256.78 Transmembrane helix 2 from 37 (in) to 52 (out) : -633.542 Transmembrane helix 3 from 56 (out) to 71 (in) : -644.712 Transmembrane helix 4 from 75 (in) to 90 (out) : -1013.83 Transmembrane helix 5 from 94 (out) to 109 (in) : -1179.32 Transmembrane helix 6 from 113 (in) to 128 (out) : -763.605 Transmembrane helix 7 from 161 (out) to 176 (in) : -865.46 Transmembrane helix 8 from 180 (in) to 195 (out) : -785.7 Transmembrane helix 9 from 199 (out) to 214 (in) : -964.254 Transmembrane helix 10 from 218 (in) to 233 (out) : -582.338 Transmembrane helix 11 from 237 (out) to 252 (in) : -358.696 Transmembrane helix 12 from 278 (in) to 293 (out) : -885.494 Transmembrane helix 13 from 297 (out) to 312 (in) : -464.943 Transmembrane helix 14 from 316 (in) to 331 (out) : -898.652 Transmembrane helix 15 from 335 (out) to 350 (in) : -793.735 Transmembrane helix 16 from 368 (in) to 383 (out) : -856.017 Transmembrane helix 17 from 406 (out) to 421 (in) : -852.082 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 18 (in) to 34 (out) : 3375.86 Transmembrane helix 2 from 38 (out) to 53 (in) : -672.426 Transmembrane helix 3 from 57 (in) to 72 (out) : -663.182 Transmembrane helix 4 from 76 (out) to 91 (in) : -1039.15 Transmembrane helix 5 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 6 from 161 (out) to 176 (in) : -865.46 Transmembrane helix 7 from 180 (in) to 195 (out) : -785.7 Transmembrane helix 8 from 199 (out) to 214 (in) : -964.254 Transmembrane helix 9 from 218 (in) to 233 (out) : -582.338 Transmembrane helix 10 from 237 (out) to 252 (in) : -358.696 Transmembrane helix 11 from 278 (in) to 293 (out) : -885.494 Transmembrane helix 12 from 297 (out) to 312 (in) : -464.943 Transmembrane helix 13 from 316 (in) to 331 (out) : -898.652 Transmembrane helix 14 from 335 (out) to 350 (in) : -793.735 Transmembrane helix 15 from 368 (in) to 383 (out) : -856.017 Transmembrane helix 16 from 406 (out) to 421 (in) : -852.082 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 18 (out) to 34 (in) : 3349.94 Transmembrane helix 2 from 38 (in) to 53 (out) : -665.474 Transmembrane helix 3 from 57 (out) to 72 (in) : -650.818 Transmembrane helix 4 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 5 from 161 (out) to 176 (in) : -865.46 Transmembrane helix 6 from 180 (in) to 195 (out) : -785.7 Transmembrane helix 7 from 199 (out) to 214 (in) : -964.254 Transmembrane helix 8 from 218 (in) to 233 (out) : -582.338 Transmembrane helix 9 from 237 (out) to 252 (in) : -358.696 Transmembrane helix 10 from 278 (in) to 293 (out) : -885.494 Transmembrane helix 11 from 297 (out) to 312 (in) : -464.943 Transmembrane helix 12 from 316 (in) to 331 (out) : -898.652 Transmembrane helix 13 from 335 (out) to 350 (in) : -793.735 Transmembrane helix 14 from 368 (in) to 383 (out) : -856.017 Transmembrane helix 15 from 406 (out) to 421 (in) : -852.082 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 18 (in) to 34 (out) : 3375.86 Transmembrane helix 2 from 38 (out) to 53 (in) : -672.426 Transmembrane helix 3 from 57 (in) to 72 (out) : -663.182 Transmembrane helix 4 from 76 (out) to 91 (in) : -1039.15 Transmembrane helix 5 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 6 from 163 (out) to 178 (in) : -853.822 Transmembrane helix 7 from 182 (in) to 197 (out) : -774.267 Transmembrane helix 8 from 218 (out) to 233 (in) : -586.362 Transmembrane helix 9 from 237 (in) to 252 (out) : -361.304 Transmembrane helix 10 from 286 (out) to 301 (in) : -635.741 Transmembrane helix 11 from 305 (in) to 320 (out) : -555.951 Transmembrane helix 12 from 335 (out) to 350 (in) : -793.735 Transmembrane helix 13 from 368 (in) to 383 (out) : -856.017 Transmembrane helix 14 from 406 (out) to 421 (in) : -852.082 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 18 (out) to 34 (in) : 3349.94 Transmembrane helix 2 from 38 (in) to 53 (out) : -665.474 Transmembrane helix 3 from 57 (out) to 72 (in) : -650.818 Transmembrane helix 4 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 5 from 163 (out) to 178 (in) : -853.822 Transmembrane helix 6 from 182 (in) to 197 (out) : -774.267 Transmembrane helix 7 from 218 (out) to 233 (in) : -586.362 Transmembrane helix 8 from 237 (in) to 252 (out) : -361.304 Transmembrane helix 9 from 286 (out) to 301 (in) : -635.741 Transmembrane helix 10 from 305 (in) to 320 (out) : -555.951 Transmembrane helix 11 from 335 (out) to 350 (in) : -793.735 Transmembrane helix 12 from 368 (in) to 383 (out) : -856.017 Transmembrane helix 13 from 406 (out) to 421 (in) : -852.082 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 18 (in) to 35 (out) : 3410.21 Transmembrane helix 2 from 39 (out) to 54 (in) : -696.188 Transmembrane helix 3 from 58 (in) to 73 (out) : -672.504 Transmembrane helix 4 from 112 (out) to 127 (in) : -748.579 Transmembrane helix 5 from 175 (in) to 190 (out) : -778.794 Transmembrane helix 6 from 218 (out) to 233 (in) : -586.362 Transmembrane helix 7 from 237 (in) to 252 (out) : -361.304 Transmembrane helix 8 from 286 (out) to 301 (in) : -635.741 Transmembrane helix 9 from 305 (in) to 320 (out) : -555.951 Transmembrane helix 10 from 335 (out) to 350 (in) : -793.735 Transmembrane helix 11 from 368 (in) to 383 (out) : -856.017 Transmembrane helix 12 from 406 (out) to 421 (in) : -852.082 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 18 (out) to 34 (in) : 3349.94 Transmembrane helix 2 from 38 (in) to 53 (out) : -665.474 Transmembrane helix 3 from 57 (out) to 72 (in) : -650.818 Transmembrane helix 4 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 5 from 182 (out) to 197 (in) : -774.033 Transmembrane helix 6 from 218 (in) to 233 (out) : -582.338 Transmembrane helix 7 from 237 (out) to 252 (in) : -358.696 Transmembrane helix 8 from 288 (in) to 303 (out) : -570.941 Transmembrane helix 9 from 307 (out) to 322 (in) : -610.85 Transmembrane helix 10 from 334 (in) to 349 (out) : -797.069 Transmembrane helix 11 from 406 (out) to 421 (in) : -852.082 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 18 (in) to 35 (out) : 3410.21 Transmembrane helix 2 from 39 (out) to 54 (in) : -696.188 Transmembrane helix 3 from 58 (in) to 73 (out) : -672.504 Transmembrane helix 4 from 112 (out) to 127 (in) : -748.579 Transmembrane helix 5 from 175 (in) to 190 (out) : -778.794 Transmembrane helix 6 from 218 (out) to 233 (in) : -586.362 Transmembrane helix 7 from 237 (in) to 252 (out) : -361.304 Transmembrane helix 8 from 286 (out) to 301 (in) : -635.741 Transmembrane helix 9 from 305 (in) to 320 (out) : -555.951 Transmembrane helix 10 from 335 (out) to 350 (in) : -793.735 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 18 (out) to 34 (in) : 3349.94 Transmembrane helix 2 from 38 (in) to 53 (out) : -665.474 Transmembrane helix 3 from 57 (out) to 72 (in) : -650.818 Transmembrane helix 4 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 5 from 182 (out) to 197 (in) : -774.033 Transmembrane helix 6 from 218 (in) to 233 (out) : -582.338 Transmembrane helix 7 from 237 (out) to 252 (in) : -358.696 Transmembrane helix 8 from 288 (in) to 303 (out) : -570.941 Transmembrane helix 9 from 307 (out) to 322 (in) : -610.85 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 18 (in) to 35 (out) : 3410.21 Transmembrane helix 2 from 50 (out) to 65 (in) : -615.916 Transmembrane helix 3 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 4 from 182 (out) to 197 (in) : -774.033 Transmembrane helix 5 from 218 (in) to 233 (out) : -582.338 Transmembrane helix 6 from 237 (out) to 252 (in) : -358.696 Transmembrane helix 7 from 288 (in) to 303 (out) : -570.941 Transmembrane helix 8 from 307 (out) to 322 (in) : -610.85 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 18 (out) to 34 (in) : 3349.94 Transmembrane helix 2 from 38 (in) to 53 (out) : -665.474 Transmembrane helix 3 from 57 (out) to 72 (in) : -650.818 Transmembrane helix 4 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 5 from 217 (out) to 232 (in) : -602.141 Transmembrane helix 6 from 236 (in) to 251 (out) : -346.088 Transmembrane helix 7 from 297 (out) to 312 (in) : -464.943 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 18 (in) to 35 (out) : 3410.21 Transmembrane helix 2 from 50 (out) to 65 (in) : -615.916 Transmembrane helix 3 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 4 from 217 (out) to 232 (in) : -602.141 Transmembrane helix 5 from 236 (in) to 251 (out) : -346.088 Transmembrane helix 6 from 297 (out) to 312 (in) : -464.943 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 18 (out) to 35 (in) : 3381.19 Transmembrane helix 2 from 49 (in) to 64 (out) : -615.793 Transmembrane helix 3 from 217 (out) to 232 (in) : -602.141 Transmembrane helix 4 from 236 (in) to 251 (out) : -346.088 Transmembrane helix 5 from 297 (out) to 312 (in) : -464.943 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 18 (in) to 35 (out) : 3410.21 Transmembrane helix 2 from 50 (out) to 65 (in) : -615.916 Transmembrane helix 3 from 234 (in) to 249 (out) : -333.987 Transmembrane helix 4 from 297 (out) to 312 (in) : -464.943 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 18 (out) to 35 (in) : 3381.19 Transmembrane helix 2 from 234 (in) to 249 (out) : -333.987 Transmembrane helix 3 from 297 (out) to 312 (in) : -464.943 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 18 (in) to 35 (out) : 3410.21 Transmembrane helix 2 from 234 (out) to 249 (in) : -333.513 Score = -100000.000000 Processing 28 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -564.823 Transmembrane helix 2 from 23 (in) to 38 (out) : 2693.13 Transmembrane helix 3 from 42 (out) to 57 (in) : -684.643 Transmembrane helix 4 from 61 (in) to 76 (out) : -700.633 Transmembrane helix 5 from 80 (out) to 95 (in) : -1115.11 Transmembrane helix 6 from 99 (in) to 114 (out) : -1101.82 Transmembrane helix 7 from 118 (out) to 133 (in) : -1004.39 Transmembrane helix 8 from 139 (in) to 154 (out) : -1407.72 Transmembrane helix 9 from 158 (out) to 173 (in) : -900.221 Transmembrane helix 10 from 177 (in) to 192 (out) : -787.575 Transmembrane helix 11 from 196 (out) to 211 (in) : -931.26 Transmembrane helix 12 from 215 (in) to 230 (out) : -629.532 Transmembrane helix 13 from 234 (out) to 249 (in) : -333.513 Transmembrane helix 14 from 253 (in) to 268 (out) : -1397.92 Transmembrane helix 15 from 272 (out) to 287 (in) : -1069.65 Transmembrane helix 16 from 291 (in) to 306 (out) : -521.094 Transmembrane helix 17 from 310 (out) to 325 (in) : -708.732 Transmembrane helix 18 from 329 (in) to 344 (out) : -867.575 Transmembrane helix 19 from 348 (out) to 363 (in) : -1092.4 Transmembrane helix 20 from 367 (in) to 382 (out) : -860.386 Transmembrane helix 21 from 386 (out) to 401 (in) : -1433.87 Transmembrane helix 22 from 405 (in) to 420 (out) : -865.049 Transmembrane helix 23 from 424 (out) to 439 (in) : -1591.63 Transmembrane helix 24 from 443 (in) to 458 (out) : -1392.49 Transmembrane helix 25 from 468 (out) to 483 (in) : -1719.29 Transmembrane helix 26 from 487 (in) to 502 (out) : -1394.71 Transmembrane helix 27 from 506 (out) to 521 (in) : -1417.09 Transmembrane helix 28 from 527 (in) to 542 (out) : -1495.76 Score = -100000.000000 Processing 27 helices: Transmembrane helix 1 from 5 (in) to 20 (out) : -290.608 Transmembrane helix 2 from 24 (out) to 39 (in) : 2430.56 Transmembrane helix 3 from 43 (in) to 58 (out) : -663.017 Transmembrane helix 4 from 62 (out) to 77 (in) : -701.076 Transmembrane helix 5 from 81 (in) to 96 (out) : -1131.47 Transmembrane helix 6 from 100 (out) to 115 (in) : -1071.61 Transmembrane helix 7 from 119 (in) to 134 (out) : -1061.1 Transmembrane helix 8 from 139 (out) to 154 (in) : -1402.98 Transmembrane helix 9 from 158 (in) to 173 (out) : -902.279 Transmembrane helix 10 from 177 (out) to 192 (in) : -781.425 Transmembrane helix 11 from 196 (in) to 211 (out) : -935.74 Transmembrane helix 12 from 215 (out) to 230 (in) : -631.068 Transmembrane helix 13 from 234 (in) to 249 (out) : -333.987 Transmembrane helix 14 from 253 (out) to 268 (in) : -1397.18 Transmembrane helix 15 from 272 (in) to 287 (out) : -1064.85 Transmembrane helix 16 from 291 (out) to 306 (in) : -529.906 Transmembrane helix 17 from 310 (in) to 325 (out) : -710.668 Transmembrane helix 18 from 329 (out) to 344 (in) : -866.325 Transmembrane helix 19 from 348 (in) to 363 (out) : -1095.1 Transmembrane helix 20 from 367 (out) to 382 (in) : -867.714 Transmembrane helix 21 from 386 (in) to 401 (out) : -1434.73 Transmembrane helix 22 from 405 (out) to 420 (in) : -864.251 Transmembrane helix 23 from 424 (in) to 439 (out) : -1591.37 Transmembrane helix 24 from 443 (out) to 458 (in) : -1386.01 Transmembrane helix 25 from 482 (in) to 497 (out) : -1355.94 Transmembrane helix 26 from 506 (out) to 521 (in) : -1417.09 Transmembrane helix 27 from 527 (in) to 542 (out) : -1495.76 Score = -100000.000000 Processing 26 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -564.823 Transmembrane helix 2 from 23 (in) to 38 (out) : 2693.13 Transmembrane helix 3 from 42 (out) to 57 (in) : -684.643 Transmembrane helix 4 from 61 (in) to 76 (out) : -700.633 Transmembrane helix 5 from 80 (out) to 95 (in) : -1115.11 Transmembrane helix 6 from 99 (in) to 114 (out) : -1101.82 Transmembrane helix 7 from 118 (out) to 133 (in) : -1004.39 Transmembrane helix 8 from 144 (in) to 159 (out) : -1257.21 Transmembrane helix 9 from 163 (out) to 178 (in) : -853.822 Transmembrane helix 10 from 182 (in) to 197 (out) : -774.267 Transmembrane helix 11 from 201 (out) to 216 (in) : -971.539 Transmembrane helix 12 from 220 (in) to 235 (out) : -568.134 Transmembrane helix 13 from 239 (out) to 254 (in) : -426.434 Transmembrane helix 14 from 259 (in) to 274 (out) : -1415.74 Transmembrane helix 15 from 278 (out) to 293 (in) : -879.506 Transmembrane helix 16 from 297 (in) to 312 (out) : -467.457 Transmembrane helix 17 from 316 (out) to 331 (in) : -886.348 Transmembrane helix 18 from 335 (in) to 350 (out) : -797.065 Transmembrane helix 19 from 354 (out) to 369 (in) : -1142.07 Transmembrane helix 20 from 373 (in) to 388 (out) : -966.301 Transmembrane helix 21 from 395 (out) to 410 (in) : -1207.03 Transmembrane helix 22 from 414 (in) to 429 (out) : -1032.68 Transmembrane helix 23 from 441 (out) to 456 (in) : -1385.84 Transmembrane helix 24 from 482 (in) to 497 (out) : -1355.94 Transmembrane helix 25 from 506 (out) to 521 (in) : -1417.09 Transmembrane helix 26 from 527 (in) to 542 (out) : -1495.76 Score = -100000.000000 Processing 25 helices: Transmembrane helix 1 from 18 (in) to 34 (out) : 3375.86 Transmembrane helix 2 from 38 (out) to 53 (in) : -672.426 Transmembrane helix 3 from 57 (in) to 72 (out) : -663.182 Transmembrane helix 4 from 76 (out) to 91 (in) : -1039.15 Transmembrane helix 5 from 95 (in) to 110 (out) : -1169.59 Transmembrane helix 6 from 114 (out) to 129 (in) : -796.663 Transmembrane helix 7 from 144 (in) to 159 (out) : -1257.21 Transmembrane helix 8 from 163 (out) to 178 (in) : -853.822 Transmembrane helix 9 from 182 (in) to 197 (out) : -774.267 Transmembrane helix 10 from 201 (out) to 216 (in) : -971.539 Transmembrane helix 11 from 220 (in) to 235 (out) : -568.134 Transmembrane helix 12 from 239 (out) to 254 (in) : -426.434 Transmembrane helix 13 from 259 (in) to 274 (out) : -1415.74 Transmembrane helix 14 from 278 (out) to 293 (in) : -879.506 Transmembrane helix 15 from 297 (in) to 312 (out) : -467.457 Transmembrane helix 16 from 316 (out) to 331 (in) : -886.348 Transmembrane helix 17 from 335 (in) to 350 (out) : -797.065 Transmembrane helix 18 from 354 (out) to 369 (in) : -1142.07 Transmembrane helix 19 from 373 (in) to 388 (out) : -966.301 Transmembrane helix 20 from 395 (out) to 410 (in) : -1207.03 Transmembrane helix 21 from 414 (in) to 429 (out) : -1032.68 Transmembrane helix 22 from 441 (out) to 456 (in) : -1385.84 Transmembrane helix 23 from 482 (in) to 497 (out) : -1355.94 Transmembrane helix 24 from 506 (out) to 521 (in) : -1417.09 Transmembrane helix 25 from 527 (in) to 542 (out) : -1495.76 Score = -100000.000000 Processing 24 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : 3256.78 Transmembrane helix 2 from 37 (in) to 52 (out) : -633.542 Transmembrane helix 3 from 56 (out) to 71 (in) : -644.712 Transmembrane helix 4 from 75 (in) to 90 (out) : -1013.83 Transmembrane helix 5 from 94 (out) to 109 (in) : -1179.32 Transmembrane helix 6 from 113 (in) to 128 (out) : -763.605 Transmembrane helix 7 from 144 (out) to 159 (in) : -1260.19 Transmembrane helix 8 from 163 (in) to 178 (out) : -848.778 Transmembrane helix 9 from 182 (out) to 197 (in) : -774.033 Transmembrane helix 10 from 201 (in) to 216 (out) : -973.761 Transmembrane helix 11 from 220 (out) to 235 (in) : -572.866 Transmembrane helix 12 from 239 (in) to 254 (out) : -430.566 Transmembrane helix 13 from 273 (out) to 288 (in) : -1031.13 Transmembrane helix 14 from 292 (in) to 307 (out) : -508.813 Transmembrane helix 15 from 311 (out) to 326 (in) : -743.785 Transmembrane helix 16 from 330 (in) to 345 (out) : -848.518 Transmembrane helix 17 from 349 (out) to 364 (in) : -1104.35 Transmembrane helix 18 from 368 (in) to 383 (out) : -856.017 Transmembrane helix 19 from 395 (out) to 410 (in) : -1207.03 Transmembrane helix 20 from 414 (in) to 429 (out) : -1032.68 Transmembrane helix 21 from 441 (out) to 456 (in) : -1385.84 Transmembrane helix 22 from 482 (in) to 497 (out) : -1355.94 Transmembrane helix 23 from 506 (out) to 521 (in) : -1417.09 Transmembrane helix 24 from 527 (in) to 542 (out) : -1495.76 Score = -100000.000000 Processing 23 helices: Transmembrane helix 1 from 18 (in) to 34 (out) : 3375.86 Transmembrane helix 2 from 38 (out) to 53 (in) : -672.426 Transmembrane helix 3 from 57 (in) to 72 (out) : -663.182 Transmembrane helix 4 from 76 (out) to 91 (in) : -1039.15 Transmembrane helix 5 from 95 (in) to 110 (out) : -1169.59 Transmembrane helix 6 from 114 (out) to 129 (in) : -796.663 Transmembrane helix 7 from 144 (in) to 159 (out) : -1257.21 Transmembrane helix 8 from 163 (out) to 178 (in) : -853.822 Transmembrane helix 9 from 182 (in) to 197 (out) : -774.267 Transmembrane helix 10 from 201 (out) to 216 (in) : -971.539 Transmembrane helix 11 from 220 (in) to 235 (out) : -568.134 Transmembrane helix 12 from 239 (out) to 254 (in) : -426.434 Transmembrane helix 13 from 259 (in) to 274 (out) : -1415.74 Transmembrane helix 14 from 278 (out) to 293 (in) : -879.506 Transmembrane helix 15 from 297 (in) to 312 (out) : -467.457 Transmembrane helix 16 from 316 (out) to 331 (in) : -886.348 Transmembrane helix 17 from 335 (in) to 350 (out) : -797.065 Transmembrane helix 18 from 354 (out) to 369 (in) : -1142.07 Transmembrane helix 19 from 373 (in) to 388 (out) : -966.301 Transmembrane helix 20 from 395 (out) to 410 (in) : -1207.03 Transmembrane helix 21 from 414 (in) to 429 (out) : -1032.68 Transmembrane helix 22 from 441 (out) to 456 (in) : -1385.84 Transmembrane helix 23 from 482 (in) to 497 (out) : -1355.94 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : 3256.78 Transmembrane helix 2 from 37 (in) to 52 (out) : -633.542 Transmembrane helix 3 from 56 (out) to 71 (in) : -644.712 Transmembrane helix 4 from 75 (in) to 90 (out) : -1013.83 Transmembrane helix 5 from 94 (out) to 109 (in) : -1179.32 Transmembrane helix 6 from 113 (in) to 128 (out) : -763.605 Transmembrane helix 7 from 144 (out) to 159 (in) : -1260.19 Transmembrane helix 8 from 163 (in) to 178 (out) : -848.778 Transmembrane helix 9 from 182 (out) to 197 (in) : -774.033 Transmembrane helix 10 from 201 (in) to 216 (out) : -973.761 Transmembrane helix 11 from 220 (out) to 235 (in) : -572.866 Transmembrane helix 12 from 239 (in) to 254 (out) : -430.566 Transmembrane helix 13 from 273 (out) to 288 (in) : -1031.13 Transmembrane helix 14 from 292 (in) to 307 (out) : -508.813 Transmembrane helix 15 from 311 (out) to 326 (in) : -743.785 Transmembrane helix 16 from 330 (in) to 345 (out) : -848.518 Transmembrane helix 17 from 349 (out) to 364 (in) : -1104.35 Transmembrane helix 18 from 368 (in) to 383 (out) : -856.017 Transmembrane helix 19 from 395 (out) to 410 (in) : -1207.03 Transmembrane helix 20 from 414 (in) to 429 (out) : -1032.68 Transmembrane helix 21 from 441 (out) to 456 (in) : -1385.84 Transmembrane helix 22 from 482 (in) to 497 (out) : -1355.94 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 18 (in) to 34 (out) : 3375.86 Transmembrane helix 2 from 38 (out) to 53 (in) : -672.426 Transmembrane helix 3 from 57 (in) to 72 (out) : -663.182 Transmembrane helix 4 from 76 (out) to 91 (in) : -1039.15 Transmembrane helix 5 from 95 (in) to 110 (out) : -1169.59 Transmembrane helix 6 from 114 (out) to 129 (in) : -796.663 Transmembrane helix 7 from 144 (in) to 159 (out) : -1257.21 Transmembrane helix 8 from 163 (out) to 178 (in) : -853.822 Transmembrane helix 9 from 182 (in) to 197 (out) : -774.267 Transmembrane helix 10 from 201 (out) to 216 (in) : -971.539 Transmembrane helix 11 from 220 (in) to 235 (out) : -568.134 Transmembrane helix 12 from 239 (out) to 254 (in) : -426.434 Transmembrane helix 13 from 268 (in) to 283 (out) : -1149.99 Transmembrane helix 14 from 287 (out) to 302 (in) : -610.602 Transmembrane helix 15 from 306 (in) to 321 (out) : -584.107 Transmembrane helix 16 from 325 (out) to 340 (in) : -914.109 Transmembrane helix 17 from 344 (in) to 359 (out) : -991.601 Transmembrane helix 18 from 368 (out) to 383 (in) : -863.983 Transmembrane helix 19 from 394 (in) to 409 (out) : -1250.32 Transmembrane helix 20 from 413 (out) to 428 (in) : -975.292 Transmembrane helix 21 from 482 (in) to 497 (out) : -1355.94 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : 3256.78 Transmembrane helix 2 from 37 (in) to 52 (out) : -633.542 Transmembrane helix 3 from 56 (out) to 71 (in) : -644.712 Transmembrane helix 4 from 75 (in) to 90 (out) : -1013.83 Transmembrane helix 5 from 94 (out) to 109 (in) : -1179.32 Transmembrane helix 6 from 113 (in) to 128 (out) : -763.605 Transmembrane helix 7 from 144 (out) to 159 (in) : -1260.19 Transmembrane helix 8 from 163 (in) to 178 (out) : -848.778 Transmembrane helix 9 from 182 (out) to 197 (in) : -774.033 Transmembrane helix 10 from 201 (in) to 216 (out) : -973.761 Transmembrane helix 11 from 220 (out) to 235 (in) : -572.866 Transmembrane helix 12 from 239 (in) to 254 (out) : -430.566 Transmembrane helix 13 from 273 (out) to 288 (in) : -1031.13 Transmembrane helix 14 from 292 (in) to 307 (out) : -508.813 Transmembrane helix 15 from 311 (out) to 326 (in) : -743.785 Transmembrane helix 16 from 330 (in) to 345 (out) : -848.518 Transmembrane helix 17 from 349 (out) to 364 (in) : -1104.35 Transmembrane helix 18 from 368 (in) to 383 (out) : -856.017 Transmembrane helix 19 from 406 (out) to 421 (in) : -852.082 Transmembrane helix 20 from 483 (in) to 498 (out) : -1356.97 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 18 (in) to 34 (out) : 3375.86 Transmembrane helix 2 from 38 (out) to 53 (in) : -672.426 Transmembrane helix 3 from 57 (in) to 72 (out) : -663.182 Transmembrane helix 4 from 76 (out) to 91 (in) : -1039.15 Transmembrane helix 5 from 95 (in) to 110 (out) : -1169.59 Transmembrane helix 6 from 114 (out) to 129 (in) : -796.663 Transmembrane helix 7 from 159 (in) to 174 (out) : -892.228 Transmembrane helix 8 from 178 (out) to 193 (in) : -778.129 Transmembrane helix 9 from 197 (in) to 212 (out) : -945.531 Transmembrane helix 10 from 218 (out) to 233 (in) : -586.362 Transmembrane helix 11 from 237 (in) to 252 (out) : -361.304 Transmembrane helix 12 from 273 (out) to 288 (in) : -1031.13 Transmembrane helix 13 from 292 (in) to 307 (out) : -508.813 Transmembrane helix 14 from 311 (out) to 326 (in) : -743.785 Transmembrane helix 15 from 330 (in) to 345 (out) : -848.518 Transmembrane helix 16 from 349 (out) to 364 (in) : -1104.35 Transmembrane helix 17 from 368 (in) to 383 (out) : -856.017 Transmembrane helix 18 from 406 (out) to 421 (in) : -852.082 Transmembrane helix 19 from 483 (in) to 498 (out) : -1356.97 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : 3256.78 Transmembrane helix 2 from 37 (in) to 52 (out) : -633.542 Transmembrane helix 3 from 56 (out) to 71 (in) : -644.712 Transmembrane helix 4 from 75 (in) to 90 (out) : -1013.83 Transmembrane helix 5 from 94 (out) to 109 (in) : -1179.32 Transmembrane helix 6 from 113 (in) to 128 (out) : -763.605 Transmembrane helix 7 from 161 (out) to 176 (in) : -865.46 Transmembrane helix 8 from 180 (in) to 195 (out) : -785.7 Transmembrane helix 9 from 199 (out) to 214 (in) : -964.254 Transmembrane helix 10 from 218 (in) to 233 (out) : -582.338 Transmembrane helix 11 from 237 (out) to 252 (in) : -358.696 Transmembrane helix 12 from 268 (in) to 283 (out) : -1149.99 Transmembrane helix 13 from 287 (out) to 302 (in) : -610.602 Transmembrane helix 14 from 306 (in) to 321 (out) : -584.107 Transmembrane helix 15 from 325 (out) to 340 (in) : -914.109 Transmembrane helix 16 from 344 (in) to 359 (out) : -991.601 Transmembrane helix 17 from 368 (out) to 383 (in) : -863.983 Transmembrane helix 18 from 406 (in) to 421 (out) : -855.218 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 18 (in) to 34 (out) : 3375.86 Transmembrane helix 2 from 38 (out) to 53 (in) : -672.426 Transmembrane helix 3 from 57 (in) to 72 (out) : -663.182 Transmembrane helix 4 from 76 (out) to 91 (in) : -1039.15 Transmembrane helix 5 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 6 from 161 (out) to 176 (in) : -865.46 Transmembrane helix 7 from 180 (in) to 195 (out) : -785.7 Transmembrane helix 8 from 199 (out) to 214 (in) : -964.254 Transmembrane helix 9 from 218 (in) to 233 (out) : -582.338 Transmembrane helix 10 from 237 (out) to 252 (in) : -358.696 Transmembrane helix 11 from 268 (in) to 283 (out) : -1149.99 Transmembrane helix 12 from 287 (out) to 302 (in) : -610.602 Transmembrane helix 13 from 306 (in) to 321 (out) : -584.107 Transmembrane helix 14 from 325 (out) to 340 (in) : -914.109 Transmembrane helix 15 from 344 (in) to 359 (out) : -991.601 Transmembrane helix 16 from 368 (out) to 383 (in) : -863.983 Transmembrane helix 17 from 406 (in) to 421 (out) : -855.218 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 18 (out) to 34 (in) : 3349.94 Transmembrane helix 2 from 38 (in) to 53 (out) : -665.474 Transmembrane helix 3 from 57 (out) to 72 (in) : -650.818 Transmembrane helix 4 from 76 (in) to 91 (out) : -1054.85 Transmembrane helix 5 from 112 (out) to 127 (in) : -748.579 Transmembrane helix 6 from 159 (in) to 174 (out) : -892.228 Transmembrane helix 7 from 178 (out) to 193 (in) : -778.129 Transmembrane helix 8 from 197 (in) to 212 (out) : -945.531 Transmembrane helix 9 from 218 (out) to 233 (in) : -586.362 Transmembrane helix 10 from 237 (in) to 252 (out) : -361.304 Transmembrane helix 11 from 278 (out) to 293 (in) : -879.506 Transmembrane helix 12 from 297 (in) to 312 (out) : -467.457 Transmembrane helix 13 from 316 (out) to 331 (in) : -886.348 Transmembrane helix 14 from 335 (in) to 350 (out) : -797.065 Transmembrane helix 15 from 368 (out) to 383 (in) : -863.983 Transmembrane helix 16 from 406 (in) to 421 (out) : -855.218 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 18 (in) to 34 (out) : 3375.86 Transmembrane helix 2 from 38 (out) to 53 (in) : -672.426 Transmembrane helix 3 from 57 (in) to 72 (out) : -663.182 Transmembrane helix 4 from 76 (out) to 91 (in) : -1039.15 Transmembrane helix 5 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 6 from 161 (out) to 176 (in) : -865.46 Transmembrane helix 7 from 180 (in) to 195 (out) : -785.7 Transmembrane helix 8 from 199 (out) to 214 (in) : -964.254 Transmembrane helix 9 from 218 (in) to 233 (out) : -582.338 Transmembrane helix 10 from 237 (out) to 252 (in) : -358.696 Transmembrane helix 11 from 288 (in) to 303 (out) : -570.941 Transmembrane helix 12 from 307 (out) to 322 (in) : -610.85 Transmembrane helix 13 from 334 (in) to 349 (out) : -797.069 Transmembrane helix 14 from 368 (out) to 383 (in) : -863.983 Transmembrane helix 15 from 406 (in) to 421 (out) : -855.218 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 18 (out) to 34 (in) : 3349.94 Transmembrane helix 2 from 38 (in) to 53 (out) : -665.474 Transmembrane helix 3 from 57 (out) to 72 (in) : -650.818 Transmembrane helix 4 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 5 from 161 (out) to 176 (in) : -865.46 Transmembrane helix 6 from 180 (in) to 195 (out) : -785.7 Transmembrane helix 7 from 199 (out) to 214 (in) : -964.254 Transmembrane helix 8 from 218 (in) to 233 (out) : -582.338 Transmembrane helix 9 from 237 (out) to 252 (in) : -358.696 Transmembrane helix 10 from 288 (in) to 303 (out) : -570.941 Transmembrane helix 11 from 307 (out) to 322 (in) : -610.85 Transmembrane helix 12 from 334 (in) to 349 (out) : -797.069 Transmembrane helix 13 from 368 (out) to 383 (in) : -863.983 Transmembrane helix 14 from 406 (in) to 421 (out) : -855.218 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 18 (in) to 35 (out) : 3410.21 Transmembrane helix 2 from 39 (out) to 54 (in) : -696.188 Transmembrane helix 3 from 58 (in) to 73 (out) : -672.504 Transmembrane helix 4 from 112 (out) to 127 (in) : -748.579 Transmembrane helix 5 from 163 (in) to 178 (out) : -848.778 Transmembrane helix 6 from 182 (out) to 197 (in) : -774.033 Transmembrane helix 7 from 218 (in) to 233 (out) : -582.338 Transmembrane helix 8 from 237 (out) to 252 (in) : -358.696 Transmembrane helix 9 from 288 (in) to 303 (out) : -570.941 Transmembrane helix 10 from 307 (out) to 322 (in) : -610.85 Transmembrane helix 11 from 334 (in) to 349 (out) : -797.069 Transmembrane helix 12 from 368 (out) to 383 (in) : -863.983 Transmembrane helix 13 from 406 (in) to 421 (out) : -855.218 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 18 (out) to 34 (in) : 3349.94 Transmembrane helix 2 from 38 (in) to 53 (out) : -665.474 Transmembrane helix 3 from 57 (out) to 72 (in) : -650.818 Transmembrane helix 4 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 5 from 163 (out) to 178 (in) : -853.822 Transmembrane helix 6 from 182 (in) to 197 (out) : -774.267 Transmembrane helix 7 from 218 (out) to 233 (in) : -586.362 Transmembrane helix 8 from 237 (in) to 252 (out) : -361.304 Transmembrane helix 9 from 286 (out) to 301 (in) : -635.741 Transmembrane helix 10 from 305 (in) to 320 (out) : -555.951 Transmembrane helix 11 from 335 (out) to 350 (in) : -793.735 Transmembrane helix 12 from 368 (in) to 383 (out) : -856.017 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 18 (in) to 35 (out) : 3410.21 Transmembrane helix 2 from 39 (out) to 54 (in) : -696.188 Transmembrane helix 3 from 58 (in) to 73 (out) : -672.504 Transmembrane helix 4 from 112 (out) to 127 (in) : -748.579 Transmembrane helix 5 from 175 (in) to 190 (out) : -778.794 Transmembrane helix 6 from 218 (out) to 233 (in) : -586.362 Transmembrane helix 7 from 237 (in) to 252 (out) : -361.304 Transmembrane helix 8 from 286 (out) to 301 (in) : -635.741 Transmembrane helix 9 from 305 (in) to 320 (out) : -555.951 Transmembrane helix 10 from 335 (out) to 350 (in) : -793.735 Transmembrane helix 11 from 368 (in) to 383 (out) : -856.017 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 18 (out) to 34 (in) : 3349.94 Transmembrane helix 2 from 38 (in) to 53 (out) : -665.474 Transmembrane helix 3 from 57 (out) to 72 (in) : -650.818 Transmembrane helix 4 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 5 from 182 (out) to 197 (in) : -774.033 Transmembrane helix 6 from 218 (in) to 233 (out) : -582.338 Transmembrane helix 7 from 237 (out) to 252 (in) : -358.696 Transmembrane helix 8 from 288 (in) to 303 (out) : -570.941 Transmembrane helix 9 from 307 (out) to 322 (in) : -610.85 Transmembrane helix 10 from 334 (in) to 349 (out) : -797.069 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 18 (in) to 35 (out) : 3410.21 Transmembrane helix 2 from 50 (out) to 65 (in) : -615.916 Transmembrane helix 3 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 4 from 182 (out) to 197 (in) : -774.033 Transmembrane helix 5 from 218 (in) to 233 (out) : -582.338 Transmembrane helix 6 from 237 (out) to 252 (in) : -358.696 Transmembrane helix 7 from 288 (in) to 303 (out) : -570.941 Transmembrane helix 8 from 307 (out) to 322 (in) : -610.85 Transmembrane helix 9 from 334 (in) to 349 (out) : -797.069 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 18 (out) to 34 (in) : 3349.94 Transmembrane helix 2 from 38 (in) to 53 (out) : -665.474 Transmembrane helix 3 from 57 (out) to 72 (in) : -650.818 Transmembrane helix 4 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 5 from 217 (out) to 232 (in) : -602.141 Transmembrane helix 6 from 236 (in) to 251 (out) : -346.088 Transmembrane helix 7 from 288 (out) to 303 (in) : -585.059 Transmembrane helix 8 from 307 (in) to 322 (out) : -606.05 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 18 (in) to 35 (out) : 3410.21 Transmembrane helix 2 from 50 (out) to 65 (in) : -615.916 Transmembrane helix 3 from 111 (in) to 126 (out) : -735.602 Transmembrane helix 4 from 217 (out) to 232 (in) : -602.141 Transmembrane helix 5 from 236 (in) to 251 (out) : -346.088 Transmembrane helix 6 from 288 (out) to 303 (in) : -585.059 Transmembrane helix 7 from 307 (in) to 322 (out) : -606.05 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 18 (out) to 35 (in) : 3381.19 Transmembrane helix 2 from 49 (in) to 64 (out) : -615.793 Transmembrane helix 3 from 217 (out) to 232 (in) : -602.141 Transmembrane helix 4 from 236 (in) to 251 (out) : -346.088 Transmembrane helix 5 from 288 (out) to 303 (in) : -585.059 Transmembrane helix 6 from 307 (in) to 322 (out) : -606.05 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 18 (in) to 35 (out) : 3410.21 Transmembrane helix 2 from 50 (out) to 65 (in) : -615.916 Transmembrane helix 3 from 218 (in) to 233 (out) : -582.338 Transmembrane helix 4 from 237 (out) to 252 (in) : -358.696 Transmembrane helix 5 from 297 (in) to 312 (out) : -467.457 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 18 (out) to 35 (in) : 3381.19 Transmembrane helix 2 from 218 (in) to 233 (out) : -582.338 Transmembrane helix 3 from 237 (out) to 252 (in) : -358.696 Transmembrane helix 4 from 297 (in) to 312 (out) : -467.457 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 18 (in) to 35 (out) : 3410.21 Transmembrane helix 2 from 234 (out) to 249 (in) : -333.513 Transmembrane helix 3 from 297 (in) to 312 (out) : -467.457 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 18 (out) to 35 (in) : 3381.19 Transmembrane helix 2 from 234 (in) to 249 (out) : -333.987 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 3.45091 1 helix (-) : Score = 3.34049 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 22 helices (+) : Score = -100000 22 helices (-) : Score = -100000 23 helices (+) : Score = -100000 23 helices (-) : Score = -100000 24 helices (+) : Score = -100000 24 helices (-) : Score = -100000 25 helices (+) : Score = -100000 25 helices (-) : Score = -100000 26 helices (+) : Score = -100000 26 helices (-) : Score = -100000 27 helices (+) : Score = -100000 27 helices (-) : Score = -100000 28 helices (+) : Score = -100000 28 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 18-35 Re-entrant helices: Not detected. Helix count: 1 N-terminal: in Score: 3.45091