Processing 1 helix: Transmembrane helix 1 from 75 (in) to 105 (out) : 3267.53 Score = 3.267530 Processing 1 helix: Transmembrane helix 1 from 76 (out) to 106 (in) : 3255.18 Score = 3.255177 Processing 9 helices: Transmembrane helix 1 from 7 (in) to 22 (out) : 997.694 Transmembrane helix 2 from 26 (out) to 41 (in) : 728.081 Transmembrane helix 3 from 45 (in) to 60 (out) : 1648.79 Transmembrane helix 4 from 64 (out) to 79 (in) : 1081.34 Transmembrane helix 5 from 83 (in) to 98 (out) : 1288.09 Transmembrane helix 6 from 102 (out) to 117 (in) : 769.878 Transmembrane helix 7 from 121 (in) to 136 (out) : 1441.76 Transmembrane helix 8 from 140 (out) to 155 (in) : 1375.51 Transmembrane helix 9 from 159 (in) to 174 (out) : -1316.2 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : 1024.01 Transmembrane helix 2 from 32 (in) to 48 (out) : 1815.78 Transmembrane helix 3 from 52 (out) to 67 (in) : 1265.59 Transmembrane helix 4 from 71 (in) to 86 (out) : 1844.03 Transmembrane helix 5 from 90 (out) to 105 (in) : 1209.28 Transmembrane helix 6 from 109 (in) to 124 (out) : 558.438 Transmembrane helix 7 from 128 (out) to 143 (in) : 1466.92 Transmembrane helix 8 from 147 (in) to 162 (out) : 472.511 Score = 9.683979 Processing 7 helices: Transmembrane helix 1 from 7 (in) to 22 (out) : 997.694 Transmembrane helix 2 from 32 (out) to 48 (in) : 1802.42 Transmembrane helix 3 from 52 (in) to 68 (out) : 1248.61 Transmembrane helix 4 from 73 (out) to 91 (in) : 2251.59 Transmembrane helix 5 from 95 (in) to 112 (out) : 1409.84 Transmembrane helix 6 from 118 (out) to 136 (in) : 1485.15 Transmembrane helix 7 from 140 (in) to 155 (out) : 1367.79 Score = 10.523192 Processing 6 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : 1024.01 Transmembrane helix 2 from 32 (in) to 48 (out) : 1815.78 Transmembrane helix 3 from 52 (out) to 68 (in) : 1275.39 Transmembrane helix 4 from 73 (in) to 91 (out) : 2282.41 Transmembrane helix 5 from 95 (out) to 112 (in) : 1422.56 Transmembrane helix 6 from 121 (in) to 151 (out) : 2852.12 Score = 10.709966 Processing 5 helices: Transmembrane helix 1 from 7 (in) to 22 (out) : 997.694 Transmembrane helix 2 from 33 (out) to 63 (in) : 3060.82 Transmembrane helix 3 from 67 (in) to 91 (out) : 2312.73 Transmembrane helix 4 from 95 (out) to 112 (in) : 1422.56 Transmembrane helix 5 from 121 (in) to 151 (out) : 2852.12 Score = 10.635706 Processing 4 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : 1024.01 Transmembrane helix 2 from 33 (in) to 63 (out) : 3052.78 Transmembrane helix 3 from 76 (out) to 106 (in) : 3255.18 Transmembrane helix 4 from 121 (in) to 151 (out) : 2852.12 Score = 10.190900 Processing 3 helices: Transmembrane helix 1 from 33 (in) to 63 (out) : 3052.78 Transmembrane helix 2 from 76 (out) to 106 (in) : 3255.18 Transmembrane helix 3 from 121 (in) to 151 (out) : 2852.12 Score = 9.129277 Processing 2 helices: Transmembrane helix 1 from 33 (out) to 63 (in) : 3060.82 Transmembrane helix 2 from 75 (in) to 105 (out) : 3267.53 Score = 6.358273 Processing 9 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : 1024.01 Transmembrane helix 2 from 26 (in) to 41 (out) : 753.719 Transmembrane helix 3 from 45 (out) to 60 (in) : 1664.61 Transmembrane helix 4 from 64 (in) to 79 (out) : 1091.56 Transmembrane helix 5 from 83 (out) to 98 (in) : 1286.71 Transmembrane helix 6 from 102 (in) to 117 (out) : 761.922 Transmembrane helix 7 from 121 (out) to 136 (in) : 1434.04 Transmembrane helix 8 from 140 (in) to 155 (out) : 1367.79 Transmembrane helix 9 from 159 (out) to 174 (in) : -1317.2 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 7 (in) to 22 (out) : 997.694 Transmembrane helix 2 from 32 (out) to 48 (in) : 1802.42 Transmembrane helix 3 from 52 (in) to 67 (out) : 1240.81 Transmembrane helix 4 from 71 (out) to 86 (in) : 1818.06 Transmembrane helix 5 from 90 (in) to 105 (out) : 1199.12 Transmembrane helix 6 from 109 (out) to 124 (in) : 570.162 Transmembrane helix 7 from 128 (in) to 143 (out) : 1478.48 Transmembrane helix 8 from 147 (out) to 162 (in) : 480.989 Score = 9.560320 Processing 7 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : 1024.01 Transmembrane helix 2 from 32 (in) to 48 (out) : 1815.78 Transmembrane helix 3 from 52 (out) to 68 (in) : 1275.39 Transmembrane helix 4 from 73 (in) to 91 (out) : 2282.41 Transmembrane helix 5 from 95 (out) to 112 (in) : 1422.56 Transmembrane helix 6 from 118 (in) to 136 (out) : 1499.25 Transmembrane helix 7 from 140 (out) to 155 (in) : 1375.51 Score = 10.734808 Processing 6 helices: Transmembrane helix 1 from 7 (in) to 22 (out) : 997.694 Transmembrane helix 2 from 33 (out) to 63 (in) : 3060.82 Transmembrane helix 3 from 67 (in) to 91 (out) : 2312.73 Transmembrane helix 4 from 95 (out) to 112 (in) : 1422.56 Transmembrane helix 5 from 118 (in) to 136 (out) : 1499.25 Transmembrane helix 6 from 140 (out) to 155 (in) : 1375.51 Score = 10.660548 Processing 5 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : 1024.01 Transmembrane helix 2 from 33 (in) to 63 (out) : 3052.78 Transmembrane helix 3 from 67 (out) to 91 (in) : 2267.77 Transmembrane helix 4 from 95 (in) to 112 (out) : 1409.84 Transmembrane helix 5 from 121 (out) to 151 (in) : 2837.68 Score = 10.602293 Processing 4 helices: Transmembrane helix 1 from 7 (in) to 22 (out) : 997.694 Transmembrane helix 2 from 33 (out) to 63 (in) : 3060.82 Transmembrane helix 3 from 75 (in) to 105 (out) : 3267.53 Transmembrane helix 4 from 121 (out) to 151 (in) : 2837.68 Score = 10.156973 Processing 3 helices: Transmembrane helix 1 from 33 (out) to 63 (in) : 3060.82 Transmembrane helix 2 from 75 (in) to 105 (out) : 3267.53 Transmembrane helix 3 from 121 (out) to 151 (in) : 2837.68 Score = 9.196895 Processing 2 helices: Transmembrane helix 1 from 33 (in) to 63 (out) : 3052.78 Transmembrane helix 2 from 76 (out) to 106 (in) : 3255.18 Score = 6.277307 Summary of topology analysis: 1 helix (+) : Score = 3.26753 1 helix (-) : Score = 3.25518 2 helices (+) : Score = 6.27731 2 helices (-) : Score = 6.35827 3 helices (+) : Score = 9.12928 3 helices (-) : Score = 9.19689 4 helices (+) : Score = 10.157 4 helices (-) : Score = 10.1909 5 helices (+) : Score = 10.6357 5 helices (-) : Score = 10.6023 6 helices (+) : Score = 10.6605 6 helices (-) : Score = 10.71 7 helices (+) : Score = 10.5232 7 helices (-) : Score = 10.7348 8 helices (+) : Score = 9.56032 8 helices (-) : Score = 9.68398 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0.727 Topology: 7-22,32-48,52-68,73-91,95-112,118-136,140-155 Re-entrant helices: Not detected. Helix count: 7 N-terminal: out Score: 10.7348