BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (200 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q9JMT4 Uncharacterized membrane protein yuaF n=1 Tax=Es...   366   e-100
UniRef50_D2T9K2 Uncharacterized membrane protein yuaF n=3 Tax=Er...    44   0.004

>UniRef50_Q9JMT4 Uncharacterized membrane protein yuaF n=1 Tax=Escherichia coli K-12
           RepID=YUAF_ECOLI
          Length = 200

 Score =  366 bits (940), Expect = e-100,   Method: Composition-based stats.
 Identities = 200/200 (100%), Positives = 200/200 (100%)

Query: 1   MYCINTDCTYTKIAIEQLLHEKYFDDRRYCHSIKIYDLRFFKIEDVAYKLIQLFKERRKE 60
           MYCINTDCTYTKIAIEQLLHEKYFDDRRYCHSIKIYDLRFFKIEDVAYKLIQLFKERRKE
Sbjct: 1   MYCINTDCTYTKIAIEQLLHEKYFDDRRYCHSIKIYDLRFFKIEDVAYKLIQLFKERRKE 60

Query: 61  NIIFLCSDRFIHSKEKPRNIFFLDTKDSIRNWSRHLKKLRYCNSNLLICFLFLCGLYHIS 120
           NIIFLCSDRFIHSKEKPRNIFFLDTKDSIRNWSRHLKKLRYCNSNLLICFLFLCGLYHIS
Sbjct: 61  NIIFLCSDRFIHSKEKPRNIFFLDTKDSIRNWSRHLKKLRYCNSNLLICFLFLCGLYHIS 120

Query: 121 VFTGKKQVIIACIKKGFSFAEIVNFLNINARTLVNYLGGLTTYFILPYSDSLYKYIIDNI 180
           VFTGKKQVIIACIKKGFSFAEIVNFLNINARTLVNYLGGLTTYFILPYSDSLYKYIIDNI
Sbjct: 121 VFTGKKQVIIACIKKGFSFAEIVNFLNINARTLVNYLGGLTTYFILPYSDSLYKYIIDNI 180

Query: 181 IASSTEEYHILLTNQRRYYV 200
           IASSTEEYHILLTNQRRYYV
Sbjct: 181 IASSTEEYHILLTNQRRYYV 200


>UniRef50_D2T9K2 Uncharacterized membrane protein yuaF n=3 Tax=Erwinia
           RepID=D2T9K2_ERWPY
          Length = 200

 Score = 43.8 bits (102), Expect = 0.004,   Method: Composition-based stats.
 Identities = 45/199 (22%), Positives = 88/199 (44%), Gaps = 11/199 (5%)

Query: 1   MYCINTDCTYTKIAIEQ-LLHEKYFDDRRYCHSIKIYDLRFFKIEDVAYKLIQLFKERRK 59
           M  + +DC Y ++A+++ +L+E     + +C  + + D+R+    + A K +  F +   
Sbjct: 1   MMHVISDCVYFELAVKKTILNESKM--KEWCEKLVVIDMRYLSSPEDALKRVMTFSDLFG 58

Query: 60  ENIIFLCSDRFIHSKEKPRNIFFLDTKDSIRNWSRHLKKLRYCNSNLLICFLFLCGLYH- 118
            +   L  D    S E   N F +D +  + ++   L+ L      L  C  +L GL H 
Sbjct: 59  GHYFVLIGDLSNVSSESYENNFLIDARLGMADFVHQLRTLTLRKITLGQCTTWLTGLIHH 118

Query: 119 ------ISVFTGKKQVIIACIKKGFSFAEIVNFLNINARTLVNYLGGLTTYFILPYSDSL 172
                 IS+ T  ++ I A + K  S A + + L ++ +T+ ++L  +   +     + L
Sbjct: 119 KDKSQGISL-TNMEKKIRALVFKNLSVANMASRLMLSNKTIYSHLNNMKNKYKAHSLNFL 177

Query: 173 YKYIIDNIIASSTEEYHIL 191
           Y  I +      + E H L
Sbjct: 178 YLKIQNGESPQCSREQHEL 196


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_Q9JMT4 Uncharacterized membrane protein yuaF n=1 Tax=Es...   366   e-100

Sequences not found previously or not previously below threshold:

UniRef50_D2T9K2 Uncharacterized membrane protein yuaF n=3 Tax=Er...    44   0.004


CONVERGED!
>UniRef50_Q9JMT4 Uncharacterized membrane protein yuaF n=1 Tax=Escherichia coli K-12
           RepID=YUAF_ECOLI
          Length = 200

 Score =  366 bits (940), Expect = e-100,   Method: Composition-based stats.
 Identities = 200/200 (100%), Positives = 200/200 (100%)

Query: 1   MYCINTDCTYTKIAIEQLLHEKYFDDRRYCHSIKIYDLRFFKIEDVAYKLIQLFKERRKE 60
           MYCINTDCTYTKIAIEQLLHEKYFDDRRYCHSIKIYDLRFFKIEDVAYKLIQLFKERRKE
Sbjct: 1   MYCINTDCTYTKIAIEQLLHEKYFDDRRYCHSIKIYDLRFFKIEDVAYKLIQLFKERRKE 60

Query: 61  NIIFLCSDRFIHSKEKPRNIFFLDTKDSIRNWSRHLKKLRYCNSNLLICFLFLCGLYHIS 120
           NIIFLCSDRFIHSKEKPRNIFFLDTKDSIRNWSRHLKKLRYCNSNLLICFLFLCGLYHIS
Sbjct: 61  NIIFLCSDRFIHSKEKPRNIFFLDTKDSIRNWSRHLKKLRYCNSNLLICFLFLCGLYHIS 120

Query: 121 VFTGKKQVIIACIKKGFSFAEIVNFLNINARTLVNYLGGLTTYFILPYSDSLYKYIIDNI 180
           VFTGKKQVIIACIKKGFSFAEIVNFLNINARTLVNYLGGLTTYFILPYSDSLYKYIIDNI
Sbjct: 121 VFTGKKQVIIACIKKGFSFAEIVNFLNINARTLVNYLGGLTTYFILPYSDSLYKYIIDNI 180

Query: 181 IASSTEEYHILLTNQRRYYV 200
           IASSTEEYHILLTNQRRYYV
Sbjct: 181 IASSTEEYHILLTNQRRYYV 200


>UniRef50_D2T9K2 Uncharacterized membrane protein yuaF n=3 Tax=Erwinia
           RepID=D2T9K2_ERWPY
          Length = 200

 Score = 43.8 bits (102), Expect = 0.004,   Method: Composition-based stats.
 Identities = 45/199 (22%), Positives = 88/199 (44%), Gaps = 11/199 (5%)

Query: 1   MYCINTDCTYTKIAIEQ-LLHEKYFDDRRYCHSIKIYDLRFFKIEDVAYKLIQLFKERRK 59
           M  + +DC Y ++A+++ +L+E     + +C  + + D+R+    + A K +  F +   
Sbjct: 1   MMHVISDCVYFELAVKKTILNESKM--KEWCEKLVVIDMRYLSSPEDALKRVMTFSDLFG 58

Query: 60  ENIIFLCSDRFIHSKEKPRNIFFLDTKDSIRNWSRHLKKLRYCNSNLLICFLFLCGLYH- 118
            +   L  D    S E   N F +D +  + ++   L+ L      L  C  +L GL H 
Sbjct: 59  GHYFVLIGDLSNVSSESYENNFLIDARLGMADFVHQLRTLTLRKITLGQCTTWLTGLIHH 118

Query: 119 ------ISVFTGKKQVIIACIKKGFSFAEIVNFLNINARTLVNYLGGLTTYFILPYSDSL 172
                 IS+ T  ++ I A + K  S A + + L ++ +T+ ++L  +   +     + L
Sbjct: 119 KDKSQGISL-TNMEKKIRALVFKNLSVANMASRLMLSNKTIYSHLNNMKNKYKAHSLNFL 177

Query: 173 YKYIIDNIIASSTEEYHIL 191
           Y  I +      + E H L
Sbjct: 178 YLKIQNGESPQCSREQHEL 196


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.331    0.145    0.448 

Lambda     K      H
   0.267   0.0458    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,240,977,782
Number of Sequences: 3077464
Number of extensions: 72311122
Number of successful extensions: 233826
Number of sequences better than 1.0e-01: 8
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 233816
Number of HSP's gapped (non-prelim): 16
length of query: 200
length of database: 1,040,396,356
effective HSP length: 122
effective length of query: 78
effective length of database: 664,945,748
effective search space: 51865768344
effective search space used: 51865768344
T: 11
A: 40
X1: 16 ( 7.6 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 90 (39.1 bits)
