BLASTP 2.2.22 [Sep-27-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= batch____ (111 letters) Database: uniref50.fasta 3,077,464 sequences; 1,040,396,356 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value UniRef50_Q9Z3A0 Putative uncharacterized protein yjgW n=5 Tax=Es... 235 4e-61 >UniRef50_Q9Z3A0 Putative uncharacterized protein yjgW n=5 Tax=Escherichia coli RepID=YJGW_ECOLI Length = 111 Score = 235 bits (599), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 111/111 (100%), Positives = 111/111 (100%) Query: 1 MIRKNKWLRFQTVCRYIPLSLKNHNRLVIFVCQRIEWRYIFSTNTGHPLKNTCEMTGTDF 60 MIRKNKWLRFQTVCRYIPLSLKNHNRLVIFVCQRIEWRYIFSTNTGHPLKNTCEMTGTDF Sbjct: 1 MIRKNKWLRFQTVCRYIPLSLKNHNRLVIFVCQRIEWRYIFSTNTGHPLKNTCEMTGTDF 60 Query: 61 STDGLMRRYVTGGNNQWHTVASLHMTIMMNRIGTVADRQPKARKVKHGEMT 111 STDGLMRRYVTGGNNQWHTVASLHMTIMMNRIGTVADRQPKARKVKHGEMT Sbjct: 61 STDGLMRRYVTGGNNQWHTVASLHMTIMMNRIGTVADRQPKARKVKHGEMT 111 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: UniRef50_Q9Z3A0 Putative uncharacterized protein yjgW n=5 Tax=Es... 227 1e-58 Sequences not found previously or not previously below threshold: CONVERGED! >UniRef50_Q9Z3A0 Putative uncharacterized protein yjgW n=5 Tax=Escherichia coli RepID=YJGW_ECOLI Length = 111 Score = 227 bits (578), Expect = 1e-58, Method: Composition-based stats. Identities = 111/111 (100%), Positives = 111/111 (100%) Query: 1 MIRKNKWLRFQTVCRYIPLSLKNHNRLVIFVCQRIEWRYIFSTNTGHPLKNTCEMTGTDF 60 MIRKNKWLRFQTVCRYIPLSLKNHNRLVIFVCQRIEWRYIFSTNTGHPLKNTCEMTGTDF Sbjct: 1 MIRKNKWLRFQTVCRYIPLSLKNHNRLVIFVCQRIEWRYIFSTNTGHPLKNTCEMTGTDF 60 Query: 61 STDGLMRRYVTGGNNQWHTVASLHMTIMMNRIGTVADRQPKARKVKHGEMT 111 STDGLMRRYVTGGNNQWHTVASLHMTIMMNRIGTVADRQPKARKVKHGEMT Sbjct: 61 STDGLMRRYVTGGNNQWHTVASLHMTIMMNRIGTVADRQPKARKVKHGEMT 111 Database: uniref50.fasta Posted date: Mar 8, 2010 10:38 AM Number of letters in database: 1,040,396,356 Number of sequences in database: 3,077,464 Lambda K H 0.326 0.135 0.439 Lambda K H 0.267 0.0433 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 529,352,647 Number of Sequences: 3077464 Number of extensions: 21568702 Number of successful extensions: 44852 Number of sequences better than 1.0e-01: 1 Number of HSP's better than 0.1 without gapping: 2 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 44850 Number of HSP's gapped (non-prelim): 2 length of query: 111 length of database: 1,040,396,356 effective HSP length: 79 effective length of query: 32 effective length of database: 797,276,700 effective search space: 25512854400 effective search space used: 25512854400 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 87 (38.0 bits)