BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (167 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P28247 Putative uncharacterized protein bicB n=1 Tax=Es...   350   1e-95
UniRef50_A7MLN2 Putative uncharacterized protein n=1 Tax=Cronoba...   145   6e-34
UniRef50_C4XBA3 Putative uncharacterized protein n=1 Tax=Klebsie...   138   8e-32
UniRef50_B1EJW4 Putative uncharacterized protein n=1 Tax=Escheri...    75   1e-12

>UniRef50_P28247 Putative uncharacterized protein bicB n=1 Tax=Escherichia coli K-12
           RepID=BICB_ECOLI
          Length = 167

 Score =  350 bits (897), Expect = 1e-95,   Method: Compositional matrix adjust.
 Identities = 167/167 (100%), Positives = 167/167 (100%)

Query: 1   MMARRSNAVRPLTIISHQHQTGGINIQPPRRMQFPRYRFIKKIEHRWVIRVVRGANIALR 60
           MMARRSNAVRPLTIISHQHQTGGINIQPPRRMQFPRYRFIKKIEHRWVIRVVRGANIALR
Sbjct: 1   MMARRSNAVRPLTIISHQHQTGGINIQPPRRMQFPRYRFIKKIEHRWVIRVVRGANIALR 60

Query: 61  LIEHEVTWTVLLRQRVAIVSDIMFRKQFERCITDDFAIDGDTIAADFTPGNSTANAELLC 120
           LIEHEVTWTVLLRQRVAIVSDIMFRKQFERCITDDFAIDGDTIAADFTPGNSTANAELLC
Sbjct: 61  LIEHEVTWTVLLRQRVAIVSDIMFRKQFERCITDDFAIDGDTIAADFTPGNSTANAELLC 120

Query: 121 DKFIKSHVCDFACKNGGRALTRKSELLSAQYSGLITSLKGKRSMAYY 167
           DKFIKSHVCDFACKNGGRALTRKSELLSAQYSGLITSLKGKRSMAYY
Sbjct: 121 DKFIKSHVCDFACKNGGRALTRKSELLSAQYSGLITSLKGKRSMAYY 167


>UniRef50_A7MLN2 Putative uncharacterized protein n=1 Tax=Cronobacter sakazakii ATCC
           BAA-894 RepID=A7MLN2_ENTS8
          Length = 250

 Score =  145 bits (365), Expect = 6e-34,   Method: Compositional matrix adjust.
 Identities = 79/162 (48%), Positives = 109/162 (67%), Gaps = 11/162 (6%)

Query: 1   MMARRSNAVRPLTIISHQHQTGGINIQPPRRMQFPRYRFIKKIEHRWVIRVVRGANIALR 60
           M+ARR +AVRPL ++SHQHQ GG++IQPP  +Q  R R ++KIEHR VIR+V GA++ALR
Sbjct: 75  MVARRGDAVRPLAVVSHQHQPGGVDIQPPCGVQLMRDRLVQKIEHRRVIRIVGGADVALR 134

Query: 61  LIEHEVTWTVLLRQRVAIVSDIMFRKQFERCITDDFAIDGDTIAADFTPGNSTANAELLC 120
            ++H++   VLL +R+A++ D++ R+QF+  +  + AI  D   A+FT  NS A+A+LL 
Sbjct: 135 FVQHKIARAVLLDERIAVILDVVIRQQFKSAVFYNLAIHRDPAGANFTASNSAADAKLLG 194

Query: 121 DKFIKSH-----VCDFACKNGGRALTRKSELL----SAQYSG 153
           DKFIKSH     V D A    GRA  R S  L    S QY+G
Sbjct: 195 DKFIKSHYVYKPVVD-AVPTRGRA-KRPSICLKRNQSRQYNG 234


>UniRef50_C4XBA3 Putative uncharacterized protein n=1 Tax=Klebsiella pneumoniae
           NTUH-K2044 RepID=C4XBA3_KLEPN
          Length = 271

 Score =  138 bits (347), Expect = 8e-32,   Method: Compositional matrix adjust.
 Identities = 63/145 (43%), Positives = 99/145 (68%)

Query: 1   MMARRSNAVRPLTIISHQHQTGGINIQPPRRMQFPRYRFIKKIEHRWVIRVVRGANIALR 60
           ++ARR NA+RPL +I HQHQ GG++IQP   +Q  R+R +++I+   +I +V G ++ LR
Sbjct: 121 VVARRGNAMRPLAVIGHQHQPGGVDIQPSGGVQLMRHRLVEEIKDSRMIGIVGGTDVPLR 180

Query: 61  LIEHEVTWTVLLRQRVAIVSDIMFRKQFERCITDDFAIDGDTIAADFTPGNSTANAELLC 120
           L+EH+VT  +LL QR++++  ++ R + +R I  + A+ G+  AA+FTPGNS A+A+LL 
Sbjct: 181 LVEHKVTRAILLGQRISVILHLVLRLELKRGIFHNVAVHGNAAAANFTPGNSPADAQLLS 240

Query: 121 DKFIKSHVCDFACKNGGRALTRKSE 145
           DK IKSH    AC + G   + + E
Sbjct: 241 DKLIKSHEIFLACHSAGAGKSPEKE 265


>UniRef50_B1EJW4 Putative uncharacterized protein n=1 Tax=Escherichia albertii
           TW07627 RepID=B1EJW4_9ESCH
          Length = 40

 Score = 74.7 bits (182), Expect = 1e-12,   Method: Composition-based stats.
 Identities = 35/40 (87%), Positives = 38/40 (95%)

Query: 128 VCDFACKNGGRALTRKSELLSAQYSGLITSLKGKRSMAYY 167
           +CDFACKNGGRALTRKSELL AQYSGLITSLK K+S+AYY
Sbjct: 1   MCDFACKNGGRALTRKSELLFAQYSGLITSLKRKKSVAYY 40


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P28247 Putative uncharacterized protein bicB n=1 Tax=Es...   272   2e-72
UniRef50_C4XBA3 Putative uncharacterized protein n=1 Tax=Klebsie...   223   2e-57
UniRef50_A7MLN2 Putative uncharacterized protein n=1 Tax=Cronoba...   211   5e-54
UniRef50_B1EJW4 Putative uncharacterized protein n=1 Tax=Escheri...    70   3e-11

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P28247 Putative uncharacterized protein bicB n=1 Tax=Escherichia coli K-12
           RepID=BICB_ECOLI
          Length = 167

 Score =  272 bits (697), Expect = 2e-72,   Method: Composition-based stats.
 Identities = 167/167 (100%), Positives = 167/167 (100%)

Query: 1   MMARRSNAVRPLTIISHQHQTGGINIQPPRRMQFPRYRFIKKIEHRWVIRVVRGANIALR 60
           MMARRSNAVRPLTIISHQHQTGGINIQPPRRMQFPRYRFIKKIEHRWVIRVVRGANIALR
Sbjct: 1   MMARRSNAVRPLTIISHQHQTGGINIQPPRRMQFPRYRFIKKIEHRWVIRVVRGANIALR 60

Query: 61  LIEHEVTWTVLLRQRVAIVSDIMFRKQFERCITDDFAIDGDTIAADFTPGNSTANAELLC 120
           LIEHEVTWTVLLRQRVAIVSDIMFRKQFERCITDDFAIDGDTIAADFTPGNSTANAELLC
Sbjct: 61  LIEHEVTWTVLLRQRVAIVSDIMFRKQFERCITDDFAIDGDTIAADFTPGNSTANAELLC 120

Query: 121 DKFIKSHVCDFACKNGGRALTRKSELLSAQYSGLITSLKGKRSMAYY 167
           DKFIKSHVCDFACKNGGRALTRKSELLSAQYSGLITSLKGKRSMAYY
Sbjct: 121 DKFIKSHVCDFACKNGGRALTRKSELLSAQYSGLITSLKGKRSMAYY 167


>UniRef50_C4XBA3 Putative uncharacterized protein n=1 Tax=Klebsiella pneumoniae
           NTUH-K2044 RepID=C4XBA3_KLEPN
          Length = 271

 Score =  223 bits (568), Expect = 2e-57,   Method: Composition-based stats.
 Identities = 63/145 (43%), Positives = 99/145 (68%)

Query: 1   MMARRSNAVRPLTIISHQHQTGGINIQPPRRMQFPRYRFIKKIEHRWVIRVVRGANIALR 60
           ++ARR NA+RPL +I HQHQ GG++IQP   +Q  R+R +++I+   +I +V G ++ LR
Sbjct: 121 VVARRGNAMRPLAVIGHQHQPGGVDIQPSGGVQLMRHRLVEEIKDSRMIGIVGGTDVPLR 180

Query: 61  LIEHEVTWTVLLRQRVAIVSDIMFRKQFERCITDDFAIDGDTIAADFTPGNSTANAELLC 120
           L+EH+VT  +LL QR++++  ++ R + +R I  + A+ G+  AA+FTPGNS A+A+LL 
Sbjct: 181 LVEHKVTRAILLGQRISVILHLVLRLELKRGIFHNVAVHGNAAAANFTPGNSPADAQLLS 240

Query: 121 DKFIKSHVCDFACKNGGRALTRKSE 145
           DK IKSH    AC + G   + + E
Sbjct: 241 DKLIKSHEIFLACHSAGAGKSPEKE 265


>UniRef50_A7MLN2 Putative uncharacterized protein n=1 Tax=Cronobacter sakazakii ATCC
           BAA-894 RepID=A7MLN2_ENTS8
          Length = 250

 Score =  211 bits (538), Expect = 5e-54,   Method: Composition-based stats.
 Identities = 77/161 (47%), Positives = 107/161 (66%), Gaps = 9/161 (5%)

Query: 1   MMARRSNAVRPLTIISHQHQTGGINIQPPRRMQFPRYRFIKKIEHRWVIRVVRGANIALR 60
           M+ARR +AVRPL ++SHQHQ GG++IQPP  +Q  R R ++KIEHR VIR+V GA++ALR
Sbjct: 75  MVARRGDAVRPLAVVSHQHQPGGVDIQPPCGVQLMRDRLVQKIEHRRVIRIVGGADVALR 134

Query: 61  LIEHEVTWTVLLRQRVAIVSDIMFRKQFERCITDDFAIDGDTIAADFTPGNSTANAELLC 120
            ++H++   VLL +R+A++ D++ R+QF+  +  + AI  D   A+FT  NS A+A+LL 
Sbjct: 135 FVQHKIARAVLLDERIAVILDVVIRQQFKSAVFYNLAIHRDPAGANFTASNSAADAKLLG 194

Query: 121 DKFIKSHVCDF----ACKNGGRALTRKSELL----SAQYSG 153
           DKFIKSH        A    GRA  R S  L    S QY+G
Sbjct: 195 DKFIKSHYVYKPVVDAVPTRGRAK-RPSICLKRNQSRQYNG 234


>UniRef50_B1EJW4 Putative uncharacterized protein n=1 Tax=Escherichia albertii
           TW07627 RepID=B1EJW4_9ESCH
          Length = 40

 Score = 69.6 bits (169), Expect = 3e-11,   Method: Composition-based stats.
 Identities = 35/40 (87%), Positives = 38/40 (95%)

Query: 128 VCDFACKNGGRALTRKSELLSAQYSGLITSLKGKRSMAYY 167
           +CDFACKNGGRALTRKSELL AQYSGLITSLK K+S+AYY
Sbjct: 1   MCDFACKNGGRALTRKSELLFAQYSGLITSLKRKKSVAYY 40


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.323    0.145    0.392 

Lambda     K      H
   0.267   0.0447    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 672,688,898
Number of Sequences: 3077464
Number of extensions: 26496025
Number of successful extensions: 70558
Number of sequences better than 1.0e-01: 4
Number of HSP's better than  0.1 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 70549
Number of HSP's gapped (non-prelim): 8
length of query: 167
length of database: 1,040,396,356
effective HSP length: 119
effective length of query: 48
effective length of database: 674,178,140
effective search space: 32360550720
effective search space used: 32360550720
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.4 bits)
S2: 88 (38.4 bits)