BLASTP 2.2.22 [Sep-27-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= batch____ (73 letters) Database: uniref50.fasta 3,077,464 sequences; 1,040,396,356 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value UniRef50_P64623 Uncharacterized protein yhdV n=97 Tax=Enterobact... 149 4e-35 >UniRef50_P64623 Uncharacterized protein yhdV n=97 Tax=Enterobacteriaceae RepID=YHDV_ECO57 Length = 73 Score = 149 bits (375), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 73/73 (100%), Positives = 73/73 (100%) Query: 1 MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTTQDNWETAGAIAGGAAA 60 MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTTQDNWETAGAIAGGAAA Sbjct: 1 MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTTQDNWETAGAIAGGAAA 60 Query: 61 VAGLTMGIIALSK 73 VAGLTMGIIALSK Sbjct: 61 VAGLTMGIIALSK 73 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: UniRef50_P64623 Uncharacterized protein yhdV n=97 Tax=Enterobact... 138 5e-32 Sequences not found previously or not previously below threshold: CONVERGED! >UniRef50_P64623 Uncharacterized protein yhdV n=97 Tax=Enterobacteriaceae RepID=YHDV_ECO57 Length = 73 Score = 138 bits (348), Expect = 5e-32, Method: Composition-based stats. Identities = 73/73 (100%), Positives = 73/73 (100%) Query: 1 MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTTQDNWETAGAIAGGAAA 60 MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTTQDNWETAGAIAGGAAA Sbjct: 1 MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTTQDNWETAGAIAGGAAA 60 Query: 61 VAGLTMGIIALSK 73 VAGLTMGIIALSK Sbjct: 61 VAGLTMGIIALSK 73 Database: uniref50.fasta Posted date: Mar 8, 2010 10:38 AM Number of letters in database: 1,040,396,356 Number of sequences in database: 3,077,464 Lambda K H 0.320 0.133 0.411 Lambda K H 0.267 0.0435 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 318,518,949 Number of Sequences: 3077464 Number of extensions: 11288070 Number of successful extensions: 38111 Number of sequences better than 1.0e-01: 1 Number of HSP's better than 0.1 without gapping: 2 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 38109 Number of HSP's gapped (non-prelim): 2 length of query: 73 length of database: 1,040,396,356 effective HSP length: 44 effective length of query: 29 effective length of database: 904,987,940 effective search space: 26244650260 effective search space used: 26244650260 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 87 (38.0 bits)