Phyre 2 Results for P76297

Phyre Home | Retrieve Phyre Job Id

Email | l.a.kelley@imperial.ac.uk
Description | P76297
Date | Thu Jan 5 12:21:39 GMT 2012
Unique Job ID | 17f2fe16edaaed92

Summary |

Top model
Image coloured by rainbow N → C terminus
| Close Jmol

Model (left) based on template d1jpya_
Top template information
Fold:Cystine-knot cytokines Superfamily:Cystine-knot cytokines Family:Interleukin 17F, IL-17F
Confidence and coverage
Confidence: | 51.2% | Coverage: | 51%
66 residues ( 51% of your sequence) have been modelled with 51.2% confidence by the single highest scoring template.
You may wish to submit your sequence to Phyrealarm. This will automatically scan your sequence every week for new potential templates as they appear in the Phyre2 library.
Please note: You must be registered and logged in to use Phyrealarm.
3D viewing
Interactive 3D view in Jmol

Sequence analysis |

Secondary structure and disorder prediction |

|  | 1 | . | . | . | . | . | . | . | . | 10 | . | . | . | . | . | . | . | . | . | 20 | . | . | . | . | . | . | . | . | . | 30 | . | . | . | . | . | . | . | . | . | 40 | . | . | . | . | . | . | . | . | . | 50 | . | . | . | . | . | . | . | . | . | 60
Sequence |  | M | R | T | L | L | A | I | L | L | F | P | L | L | V | Q | A | A | G | E | G | M | W | Q | A | S | S | V | G | I | T | L | N | H | R | G | E | S | M | S | S | A | P | L | S | T | R | Q | P | A | S | G | L | M | T | L | V | A | W | R | Y
Secondary structure |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |
SS confidence |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |
Disorder |  | ? |  |  |  |  |  |  |  |  |  |  |  |  |  |  | ? | ? | ? | ? | ? | ? | ? | ? | ? | ? | ? | ? | ? |  | ? | ? | ? | ? | ? | ? |  |  |  |  | ? | ? | ? | ? | ? | ? | ? | ? | ? | ? | ? | ? |  |  |  |  |  |  |  |  |
Disorder confidence |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |
|  | . | . | . | . | . | . | . | . | . | 70 | . | . | . | . | . | . | . | . | . | 80 | . | . | . | . | . | . | . | . | . | 90 | . | . | . | . | . | . | . | . | . | 100 | . | . | . | . | . | . | . | . | . | 110 | . | . | . | . | . | . | . | . | . | 120
Sequence |  | Q | L | I | G | P | T | P | S | G | L | R | V | R | L | C | S | Q | S | R | C | V | E | L | E | G | Q | S | G | T | T | V | A | F | S | G | I | A | A | A | E | P | L | R | F | I | W | E | V | P | G | G | G | R | L | I | P | P | L | K | V
Secondary structure |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |
SS confidence |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |
Disorder |  |  |  |  |  | ? | ? | ? |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  | ? | ? | ? | ? | ? | ? | ? | ? | ? | ? | ? | ? | ? | ? |  |  |  |  |  |  |  |  |  |  |  |  |  | ? | ? |  | ? | ? | ? | ? | ? | ? | ? | ?
Disorder confidence |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |
|  | . | . | . | . | . | . | . | . | . | 130
Sequence |  | Q | R | N | E | V | I | V | N | Y | R
Secondary structure |  |  |  |  |  |  |  |  |  |  |
SS confidence |  |  |  |  |  |  |  |  |  |  |
Disorder |  | ? | ? | ? |  |  |  |  |  |  | ?
Disorder confidence |  |  |  |  |  |  |  |  |  |  |

Confidence Key
High(9) |  |  |  |  |  |  |  |  |  |  | Low (0)

? | Disordered
| Alpha helix
| Beta strand

Domain analysis |

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank | Aligned region
--- | ---
1 |
| d1jpya_ |

### PDB 1jpy chain A

Region: 58 - 127
Aligned: 66
Modelled: 70
Confidence: 51.2%
Identity: 15%
Fold: Cystine-knot cytokines
Superfamily: Cystine-knot cytokines
Family: Interleukin 17F, IL-17F

Phyre2
2 |
| c2z0bB_ |

### PDB 2z0b chain B

Region: 59 - 128
Aligned: 68
Modelled: 69
Confidence: 39.7%
Identity: 21%
PDB header:hydrolase
Chain: B: PDB Molecule:putative glycerophosphodiester phosphodiesterase 5;
PDBTitle: crystal structure of cbm20 domain of human putative2 glycerophosphodiester phosphodiesterase 5 (kiaa1434)

Phyre2
3 |
| c2kv5A_ |

### PDB 2kv5 chain A

Region: 1 - 14
Aligned: 14
Modelled: 14
Confidence: 25.5%
Identity: 29%
PDB header:toxin
Chain: A: PDB Molecule:putative uncharacterized protein rnai;
PDBTitle: solution structure of the par toxin fst in dpc micelles

Phyre2
4 |
| c2wskA_ |

### PDB 2wsk chain A

Region: 45 - 111
Aligned: 65
Modelled: 67
Confidence: 14.8%
Identity: 25%
PDB header:hydrolase
Chain: A: PDB Molecule:glycogen debranching enzyme;
PDBTitle: crystal structure of glycogen debranching enzyme glgx from2 escherichia coli k-12

Phyre2
5 |
| d2zkmx4 |

### PDB 2zkm chain X domain 4

Region: 79 - 89
Aligned: 11
Modelled: 11
Confidence: 13.7%
Identity: 55%
Fold: TIM beta/alpha-barrel
Superfamily: PLC-like phosphodiesterases
Family: Mammalian PLC

Phyre2
6 |
| d1qasa3 |

### PDB 1qas chain A domain 3

Region: 79 - 89
Aligned: 11
Modelled: 11
Confidence: 13.5%
Identity: 45%
Fold: TIM beta/alpha-barrel
Superfamily: PLC-like phosphodiesterases
Family: Mammalian PLC

Phyre2
7 |
| d1cyga2 |

### PDB 1cyg chain A domain 2

Region: 55 - 130
Aligned: 76
Modelled: 76
Confidence: 13.2%
Identity: 14%
Fold: Prealbumin-like
Superfamily: Starch-binding domain-like
Family: Starch-binding domain

Phyre2
8 |
| d1kpta_ |

### PDB 1kpt chain A

Region: 114 - 129
Aligned: 16
Modelled: 16
Confidence: 13.0%
Identity: 38%
Fold: Yeast killer toxins
Superfamily: Yeast killer toxins
Family: Virally encoded KP4 toxin

Phyre2
9 |
| c3cdzA_ |

### PDB 3cdz chain A

Region: 83 - 112
Aligned: 30
Modelled: 30
Confidence: 11.2%
Identity: 10%
PDB header:blood clotting
Chain: A: PDB Molecule:coagulation factor viii heavy chain;
PDBTitle: crystal structure of human factor viii

Phyre2
10 |
| d3bmva2 |

### PDB 3bmv chain A domain 2

Region: 54 - 130
Aligned: 77
Modelled: 77
Confidence: 10.8%
Identity: 19%
Fold: Prealbumin-like
Superfamily: Starch-binding domain-like
Family: Starch-binding domain

Phyre2
11 |
| d1a73a_ |

### PDB 1a73 chain A

Region: 52 - 84
Aligned: 33
Modelled: 33
Confidence: 10.4%
Identity: 21%
Fold: His-Me finger endonucleases
Superfamily: His-Me finger endonucleases
Family: Intron-encoded homing endonucleases

Phyre2
12 |
| c3spaA_ |

### PDB 3spa chain A

Region: 99 - 116
Aligned: 18
Modelled: 18
Confidence: 10.2%
Identity: 17%
PDB header:transferase
Chain: A: PDB Molecule:dna-directed rna polymerase, mitochondrial;
PDBTitle: crystal structure of human mitochondrial rna polymerase

Phyre2
13 |
| c3pnrB_ |

### PDB 3pnr chain B

Region: 80 - 88
Aligned: 9
Modelled: 9
Confidence: 8.8%
Identity: 56%
PDB header:hydrolase/hydrolase inhibitor
Chain: B: PDB Molecule:pbicp-c;
PDBTitle: structure of pbicp-c in complex with falcipain-2

Phyre2
14 |
| d1iz5a2 |

### PDB 1iz5 chain A domain 2

Region: 96 - 114
Aligned: 19
Modelled: 19
Confidence: 8.3%
Identity: 21%
Fold: DNA clamp
Superfamily: DNA clamp
Family: DNA polymerase processivity factor

Phyre2
15 |
| d1kula_ |

### PDB 1kul chain A

Region: 59 - 113
Aligned: 55
Modelled: 55
Confidence: 7.8%
Identity: 15%
Fold: Prealbumin-like
Superfamily: Starch-binding domain-like
Family: Starch-binding domain

Phyre2
16 |
| c3l81A_ |

### PDB 3l81 chain A

Region: 63 - 123
Aligned: 60
Modelled: 61
Confidence: 7.7%
Identity: 13%
PDB header:transport protein
Chain: A: PDB Molecule:ap-4 complex subunit mu-1;
PDBTitle: crystal structure of adaptor protein complex 4 (ap-4) mu4 subunit c-2 terminal domain, in complex with a sorting peptide from the amyloid3 precursor protein (app)

Phyre2
17 |
| c1djyB_ |

### PDB 1djy chain B

Region: 79 - 89
Aligned: 11
Modelled: 11
Confidence: 7.6%
Identity: 45%
PDB header:lipid degradation
Chain: B: PDB Molecule:phosphoinositide-specific phospholipase c,
PDBTitle: phosphoinositide-specific phospholipase c-delta1 from rat2 complexed with inositol-2,4,5-trisphosphate

Phyre2
18 |
| d1ud9a2 |

### PDB 1ud9 chain A domain 2

Region: 96 - 114
Aligned: 19
Modelled: 19
Confidence: 7.5%
Identity: 26%
Fold: DNA clamp
Superfamily: DNA clamp
Family: DNA polymerase processivity factor

Phyre2
19 |
| c2ix2A_ |

### PDB 2ix2 chain A

Region: 95 - 114
Aligned: 20
Modelled: 20
Confidence: 7.1%
Identity: 20%
PDB header:replication
Chain: A: PDB Molecule:dna polymerase sliding clamp b;
PDBTitle: crystal structure of the heterotrimeric pcna from2 sulfolobus solfataricus

Phyre2
20 |
| d1mswd_ |

### PDB 1msw chain D

Region: 98 - 116
Aligned: 19
Modelled: 19
Confidence: 7.0%
Identity: 21%
Fold: DNA/RNA polymerases
Superfamily: DNA/RNA polymerases
Family: T7 RNA polymerase

Phyre2
21 |
22 |
23 |
24 |
25 |
26 |
27 |
28 |
29 |
30 |
31 |
32 |

Detailed template information |

# | Template | Alignment Coverage | 3D Model | Confidence | % i.d. | Template Information
--- | --- | --- | --- | --- | --- | ---
1 | d1jpya_ |
|  | 51.2 | 15 | Fold:Cystine-knot cytokines Superfamily:Cystine-knot cytokines Family:Interleukin 17F, IL-17F
2 | c2z0bB_ |
|  | 39.7 | 21 | PDB header:hydrolase Chain: B: PDB Molecule:putative glycerophosphodiester phosphodiesterase 5; PDBTitle: crystal structure of cbm20 domain of human putative2 glycerophosphodiester phosphodiesterase 5 (kiaa1434)
3 | c2kv5A_ |
|  | 25.5 | 29 | PDB header:toxin Chain: A: PDB Molecule:putative uncharacterized protein rnai; PDBTitle: solution structure of the par toxin fst in dpc micelles
4 | c2wskA_ |
|  | 14.8 | 25 | PDB header:hydrolase Chain: A: PDB Molecule:glycogen debranching enzyme; PDBTitle: crystal structure of glycogen debranching enzyme glgx from2 escherichia coli k-12
5 | d2zkmx4 |
|  | 13.7 | 55 | Fold:TIM beta/alpha-barrel Superfamily:PLC-like phosphodiesterases Family:Mammalian PLC
6 | d1qasa3 |
|  | 13.5 | 45 | Fold:TIM beta/alpha-barrel Superfamily:PLC-like phosphodiesterases Family:Mammalian PLC
7 | d1cyga2 |
|  | 13.2 | 14 | Fold:Prealbumin-like Superfamily:Starch-binding domain-like Family:Starch-binding domain
8 | d1kpta_ |
|  | 13.0 | 38 | Fold:Yeast killer toxins Superfamily:Yeast killer toxins Family:Virally encoded KP4 toxin
9 | c3cdzA_ |
|  | 11.2 | 10 | PDB header:blood clotting Chain: A: PDB Molecule:coagulation factor viii heavy chain; PDBTitle: crystal structure of human factor viii
10 | d3bmva2 |
|  | 10.8 | 19 | Fold:Prealbumin-like Superfamily:Starch-binding domain-like Family:Starch-binding domain
11 | d1a73a_ |
|  | 10.4 | 21 | Fold:His-Me finger endonucleases Superfamily:His-Me finger endonucleases Family:Intron-encoded homing endonucleases
12 | c3spaA_ |
|  | 10.2 | 17 | PDB header:transferase Chain: A: PDB Molecule:dna-directed rna polymerase, mitochondrial; PDBTitle: crystal structure of human mitochondrial rna polymerase
13 | c3pnrB_ |
|  | 8.8 | 56 | PDB header:hydrolase/hydrolase inhibitor Chain: B: PDB Molecule:pbicp-c; PDBTitle: structure of pbicp-c in complex with falcipain-2
14 | d1iz5a2 |
|  | 8.3 | 21 | Fold:DNA clamp Superfamily:DNA clamp Family:DNA polymerase processivity factor
15 | d1kula_ |
|  | 7.8 | 15 | Fold:Prealbumin-like Superfamily:Starch-binding domain-like Family:Starch-binding domain
16 | c3l81A_ |
|  | 7.7 | 13 | PDB header:transport protein Chain: A: PDB Molecule:ap-4 complex subunit mu-1; PDBTitle: crystal structure of adaptor protein complex 4 (ap-4) mu4 subunit c-2 terminal domain, in complex with a sorting peptide from the amyloid3 precursor protein (app)
17 | c1djyB_ |
|  | 7.6 | 45 | PDB header:lipid degradation Chain: B: PDB Molecule:phosphoinositide-specific phospholipase c, PDBTitle: phosphoinositide-specific phospholipase c-delta1 from rat2 complexed with inositol-2,4,5-trisphosphate
18 | d1ud9a2 |
|  | 7.5 | 26 | Fold:DNA clamp Superfamily:DNA clamp Family:DNA polymerase processivity factor
19 | c2ix2A_ |
|  | 7.1 | 20 | PDB header:replication Chain: A: PDB Molecule:dna polymerase sliding clamp b; PDBTitle: crystal structure of the heterotrimeric pcna from2 sulfolobus solfataricus
20 | d1mswd_ |
|  | 7.0 | 21 | Fold:DNA/RNA polymerases Superfamily:DNA/RNA polymerases Family:T7 RNA polymerase
21 | c3tu8A_ |
| not modelled | 7.0 | 31 | PDB header:unknown function Chain: A: PDB Molecule:burkholderia lethal factor 1 (blf1); PDBTitle: crystal structure of the burkholderia lethal factor 1 (blf1)
22 | d1u7ba2 |
| not modelled | 6.8 | 26 | Fold:DNA clamp Superfamily:DNA clamp Family:DNA polymerase processivity factor
23 | c2k1iA_ |
| not modelled | 6.2 | 47 | PDB header:antimicrobial protein Chain: A: PDB Molecule:mucosal alpha-defensin; PDBTitle: synthesis, structure and activities of an oral mucosal2 alpha-defensin from rhesus macaque
24 | d1fx0b2 |
| not modelled | 6.2 | 26 | Fold:Domain of alpha and beta subunits of F1 ATP synthase-like Superfamily:N-terminal domain of alpha and beta subunits of F1 ATP synthase Family:N-terminal domain of alpha and beta subunits of F1 ATP synthase
25 | d2jdid2 |
| not modelled | 6.0 | 17 | Fold:Domain of alpha and beta subunits of F1 ATP synthase-like Superfamily:N-terminal domain of alpha and beta subunits of F1 ATP synthase Family:N-terminal domain of alpha and beta subunits of F1 ATP synthase
26 | c3ohmB_ |
| not modelled | 6.0 | 60 | PDB header:signaling protein / hydrolase Chain: B: PDB Molecule:1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase PDBTitle: crystal structure of activated g alpha q bound to its effector2 phospholipase c beta 3
27 | c2fjuB_ |
| not modelled | 5.9 | 60 | PDB header:signaling protein,apoptosis/hydrolase Chain: B: PDB Molecule:1-phosphatidylinositol-4,5-bisphosphate PDBTitle: activated rac1 bound to its effector phospholipase c beta 2
28 | d1cxla2 |
| not modelled | 5.6 | 14 | Fold:Prealbumin-like Superfamily:Starch-binding domain-like Family:Starch-binding domain
29 | d1gpla1 |
| not modelled | 5.5 | 26 | Fold:Lipase/lipooxygenase domain (PLAT/LH2 domain) Superfamily:Lipase/lipooxygenase domain (PLAT/LH2 domain) Family:Colipase-binding domain
30 | d1wb1a1 |
| not modelled | 5.4 | 11 | Fold:Reductase/isomerase/elongation factor common domain Superfamily:Translation proteins Family:Elongation factors
31 | d1mabb2 |
| not modelled | 5.3 | 17 | Fold:Domain of alpha and beta subunits of F1 ATP synthase-like Superfamily:N-terminal domain of alpha and beta subunits of F1 ATP synthase Family:N-terminal domain of alpha and beta subunits of F1 ATP synthase
32 | d1edqa1 |
| not modelled | 5.3 | 16 | Fold:Immunoglobulin-like beta-sandwich Superfamily:E set domains Family:E-set domains of sugar-utilizing enzymes

Binding site prediction |

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction |

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group
Imperial College London
Lawrence Kelley, Benjamin Jefferys
Disclaimer
Terms and Conditions
Component software
Template detection: HHpred 1.51
Secondary structure prediction: Psi-pred 2.5
Disorder prediction: Disopred 2.4
Transmembrane prediction: Memsat_SVM
Multi-template modelling and ab initio: Poing 1.0