Processing 1 helix: Transmembrane helix 1 from 236 (in) to 255 (out) : 28.72 Score = 0.040484 Processing 1 helix: Transmembrane helix 1 from 214 (out) to 229 (in) : 7.352 Score = 0.009631 Processing 13 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : -849.352 Transmembrane helix 2 from 35 (out) to 50 (in) : -1353.34 Transmembrane helix 3 from 54 (in) to 69 (out) : -684.296 Transmembrane helix 4 from 73 (out) to 88 (in) : -815.488 Transmembrane helix 5 from 92 (in) to 107 (out) : -694.092 Transmembrane helix 6 from 111 (out) to 126 (in) : -164.538 Transmembrane helix 7 from 130 (in) to 145 (out) : -1574.62 Transmembrane helix 8 from 149 (out) to 164 (in) : -512.913 Transmembrane helix 9 from 168 (in) to 183 (out) : -883.046 Transmembrane helix 10 from 187 (out) to 202 (in) : -928.579 Transmembrane helix 11 from 206 (in) to 221 (out) : -54.913 Transmembrane helix 12 from 225 (out) to 240 (in) : 11.162 Transmembrane helix 13 from 244 (in) to 259 (out) : 16.393 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -497.332 Transmembrane helix 2 from 39 (in) to 54 (out) : -1381.3 Transmembrane helix 3 from 58 (out) to 73 (in) : -609.081 Transmembrane helix 4 from 77 (in) to 92 (out) : -976.453 Transmembrane helix 5 from 96 (out) to 111 (in) : -531.635 Transmembrane helix 6 from 115 (in) to 130 (out) : -143.211 Transmembrane helix 7 from 149 (out) to 164 (in) : -512.913 Transmembrane helix 8 from 168 (in) to 183 (out) : -883.046 Transmembrane helix 9 from 187 (out) to 202 (in) : -928.579 Transmembrane helix 10 from 206 (in) to 221 (out) : -54.913 Transmembrane helix 11 from 225 (out) to 240 (in) : 11.162 Transmembrane helix 12 from 244 (in) to 259 (out) : 16.393 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -486.768 Transmembrane helix 2 from 54 (out) to 69 (in) : -700.404 Transmembrane helix 3 from 73 (in) to 88 (out) : -818.412 Transmembrane helix 4 from 96 (out) to 111 (in) : -531.635 Transmembrane helix 5 from 115 (in) to 130 (out) : -143.211 Transmembrane helix 6 from 149 (out) to 164 (in) : -512.913 Transmembrane helix 7 from 168 (in) to 183 (out) : -883.046 Transmembrane helix 8 from 187 (out) to 202 (in) : -928.579 Transmembrane helix 9 from 206 (in) to 221 (out) : -54.913 Transmembrane helix 10 from 225 (out) to 240 (in) : 11.162 Transmembrane helix 11 from 244 (in) to 259 (out) : 16.393 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -497.332 Transmembrane helix 2 from 62 (in) to 77 (out) : -525.278 Transmembrane helix 3 from 96 (out) to 111 (in) : -531.635 Transmembrane helix 4 from 115 (in) to 130 (out) : -143.211 Transmembrane helix 5 from 149 (out) to 164 (in) : -512.913 Transmembrane helix 6 from 168 (in) to 183 (out) : -883.046 Transmembrane helix 7 from 187 (out) to 202 (in) : -928.579 Transmembrane helix 8 from 206 (in) to 221 (out) : -54.913 Transmembrane helix 9 from 225 (out) to 240 (in) : 11.162 Transmembrane helix 10 from 244 (in) to 259 (out) : 16.393 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -486.768 Transmembrane helix 2 from 62 (out) to 77 (in) : -525.022 Transmembrane helix 3 from 96 (in) to 111 (out) : -524.865 Transmembrane helix 4 from 115 (out) to 130 (in) : -145.289 Transmembrane helix 5 from 150 (in) to 165 (out) : -471.819 Transmembrane helix 6 from 169 (out) to 184 (in) : -896.687 Transmembrane helix 7 from 203 (in) to 218 (out) : -51.31 Transmembrane helix 8 from 222 (out) to 237 (in) : 8.431 Transmembrane helix 9 from 241 (in) to 256 (out) : 25.197 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -497.332 Transmembrane helix 2 from 62 (in) to 77 (out) : -525.278 Transmembrane helix 3 from 96 (out) to 111 (in) : -531.635 Transmembrane helix 4 from 115 (in) to 130 (out) : -143.211 Transmembrane helix 5 from 151 (out) to 166 (in) : -446.734 Transmembrane helix 6 from 203 (in) to 218 (out) : -51.31 Transmembrane helix 7 from 222 (out) to 237 (in) : 8.431 Transmembrane helix 8 from 241 (in) to 256 (out) : 25.197 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -486.768 Transmembrane helix 2 from 62 (out) to 77 (in) : -525.022 Transmembrane helix 3 from 113 (in) to 128 (out) : -131.169 Transmembrane helix 4 from 151 (out) to 166 (in) : -446.734 Transmembrane helix 5 from 203 (in) to 218 (out) : -51.31 Transmembrane helix 6 from 222 (out) to 237 (in) : 8.431 Transmembrane helix 7 from 241 (in) to 256 (out) : 25.197 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -497.332 Transmembrane helix 2 from 113 (in) to 128 (out) : -131.169 Transmembrane helix 3 from 151 (out) to 166 (in) : -446.734 Transmembrane helix 4 from 203 (in) to 218 (out) : -51.31 Transmembrane helix 5 from 222 (out) to 237 (in) : 8.431 Transmembrane helix 6 from 241 (in) to 256 (out) : 25.197 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 113 (in) to 128 (out) : -131.169 Transmembrane helix 2 from 151 (out) to 166 (in) : -446.734 Transmembrane helix 3 from 203 (in) to 218 (out) : -51.31 Transmembrane helix 4 from 222 (out) to 237 (in) : 8.431 Transmembrane helix 5 from 241 (in) to 256 (out) : 25.197 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 114 (out) to 129 (in) : -134.598 Transmembrane helix 2 from 204 (in) to 219 (out) : -45.871 Transmembrane helix 3 from 223 (out) to 238 (in) : 9.256 Transmembrane helix 4 from 242 (in) to 257 (out) : 22.919 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 204 (in) to 219 (out) : -45.871 Transmembrane helix 2 from 223 (out) to 238 (in) : 9.256 Transmembrane helix 3 from 242 (in) to 257 (out) : 22.919 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 214 (out) to 229 (in) : 7.352 Transmembrane helix 2 from 240 (in) to 255 (out) : 25.611 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : -858.848 Transmembrane helix 2 from 35 (in) to 50 (out) : -1370.66 Transmembrane helix 3 from 54 (out) to 69 (in) : -700.404 Transmembrane helix 4 from 73 (in) to 88 (out) : -818.412 Transmembrane helix 5 from 92 (out) to 107 (in) : -691.208 Transmembrane helix 6 from 111 (in) to 126 (out) : -153.562 Transmembrane helix 7 from 130 (out) to 145 (in) : -1568.98 Transmembrane helix 8 from 149 (in) to 164 (out) : -520.787 Transmembrane helix 9 from 168 (out) to 183 (in) : -873.354 Transmembrane helix 10 from 187 (in) to 202 (out) : -903.321 Transmembrane helix 11 from 206 (out) to 221 (in) : -31.687 Transmembrane helix 12 from 225 (in) to 240 (out) : -11.162 Transmembrane helix 13 from 244 (out) to 260 (in) : -16.269 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -486.768 Transmembrane helix 2 from 39 (out) to 54 (in) : -1374.7 Transmembrane helix 3 from 58 (in) to 73 (out) : -599.019 Transmembrane helix 4 from 77 (out) to 92 (in) : -973.147 Transmembrane helix 5 from 96 (in) to 111 (out) : -524.865 Transmembrane helix 6 from 115 (out) to 130 (in) : -145.289 Transmembrane helix 7 from 149 (in) to 164 (out) : -520.787 Transmembrane helix 8 from 168 (out) to 183 (in) : -873.354 Transmembrane helix 9 from 187 (in) to 202 (out) : -903.321 Transmembrane helix 10 from 206 (out) to 221 (in) : -31.687 Transmembrane helix 11 from 225 (in) to 240 (out) : -11.162 Transmembrane helix 12 from 244 (out) to 260 (in) : -16.269 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -497.332 Transmembrane helix 2 from 54 (in) to 69 (out) : -684.296 Transmembrane helix 3 from 73 (out) to 88 (in) : -815.488 Transmembrane helix 4 from 96 (in) to 111 (out) : -524.865 Transmembrane helix 5 from 115 (out) to 130 (in) : -145.289 Transmembrane helix 6 from 149 (in) to 164 (out) : -520.787 Transmembrane helix 7 from 168 (out) to 183 (in) : -873.354 Transmembrane helix 8 from 187 (in) to 202 (out) : -903.321 Transmembrane helix 9 from 206 (out) to 221 (in) : -31.687 Transmembrane helix 10 from 225 (in) to 240 (out) : -11.162 Transmembrane helix 11 from 244 (out) to 260 (in) : -16.269 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -486.768 Transmembrane helix 2 from 62 (out) to 77 (in) : -525.022 Transmembrane helix 3 from 96 (in) to 111 (out) : -524.865 Transmembrane helix 4 from 115 (out) to 130 (in) : -145.289 Transmembrane helix 5 from 149 (in) to 164 (out) : -520.787 Transmembrane helix 6 from 168 (out) to 183 (in) : -873.354 Transmembrane helix 7 from 187 (in) to 202 (out) : -903.321 Transmembrane helix 8 from 206 (out) to 221 (in) : -31.687 Transmembrane helix 9 from 225 (in) to 240 (out) : -11.162 Transmembrane helix 10 from 244 (out) to 260 (in) : -16.269 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -497.332 Transmembrane helix 2 from 62 (in) to 77 (out) : -525.278 Transmembrane helix 3 from 96 (out) to 111 (in) : -531.635 Transmembrane helix 4 from 115 (in) to 130 (out) : -143.211 Transmembrane helix 5 from 151 (out) to 166 (in) : -446.734 Transmembrane helix 6 from 187 (in) to 202 (out) : -903.321 Transmembrane helix 7 from 206 (out) to 221 (in) : -31.687 Transmembrane helix 8 from 225 (in) to 240 (out) : -11.162 Transmembrane helix 9 from 244 (out) to 260 (in) : -16.269 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -486.768 Transmembrane helix 2 from 62 (out) to 77 (in) : -525.022 Transmembrane helix 3 from 96 (in) to 111 (out) : -524.865 Transmembrane helix 4 from 115 (out) to 130 (in) : -145.289 Transmembrane helix 5 from 151 (in) to 166 (out) : -455.966 Transmembrane helix 6 from 205 (out) to 221 (in) : -25.256 Transmembrane helix 7 from 225 (in) to 240 (out) : -11.162 Transmembrane helix 8 from 244 (out) to 260 (in) : -16.269 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -497.332 Transmembrane helix 2 from 62 (in) to 77 (out) : -525.278 Transmembrane helix 3 from 114 (out) to 129 (in) : -134.598 Transmembrane helix 4 from 151 (in) to 166 (out) : -455.966 Transmembrane helix 5 from 205 (out) to 221 (in) : -25.256 Transmembrane helix 6 from 225 (in) to 240 (out) : -11.162 Transmembrane helix 7 from 244 (out) to 260 (in) : -16.269 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -486.768 Transmembrane helix 2 from 114 (out) to 129 (in) : -134.598 Transmembrane helix 3 from 151 (in) to 166 (out) : -455.966 Transmembrane helix 4 from 205 (out) to 221 (in) : -25.256 Transmembrane helix 5 from 225 (in) to 240 (out) : -11.162 Transmembrane helix 6 from 244 (out) to 260 (in) : -16.269 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 114 (out) to 129 (in) : -134.598 Transmembrane helix 2 from 151 (in) to 166 (out) : -455.966 Transmembrane helix 3 from 205 (out) to 221 (in) : -25.256 Transmembrane helix 4 from 225 (in) to 240 (out) : -11.162 Transmembrane helix 5 from 244 (out) to 260 (in) : -16.269 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 114 (in) to 129 (out) : -130.202 Transmembrane helix 2 from 205 (out) to 221 (in) : -25.256 Transmembrane helix 3 from 225 (in) to 240 (out) : -11.162 Transmembrane helix 4 from 244 (out) to 260 (in) : -16.269 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 205 (out) to 221 (in) : -25.256 Transmembrane helix 2 from 225 (in) to 240 (out) : -11.162 Transmembrane helix 3 from 244 (out) to 260 (in) : -16.269 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 225 (in) to 240 (out) : -11.162 Transmembrane helix 2 from 244 (out) to 260 (in) : -16.269 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.040484 1 helix (-) : Score = 0.009631 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 Results: Signal peptide: 1-15 Signal score: 25.465 Topology: 236-255 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.040484