Processing 1 helix: Transmembrane helix 1 from 78 (in) to 93 (out) : -297.175 Score = -0.297175 Processing 1 helix: Transmembrane helix 1 from 78 (out) to 93 (in) : -290.225 Score = -0.290225 Processing 10 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -25036.3 Transmembrane helix 2 from 30 (out) to 45 (in) : -3326.19 Transmembrane helix 3 from 49 (in) to 64 (out) : -1238.64 Transmembrane helix 4 from 68 (out) to 83 (in) : -541.531 Transmembrane helix 5 from 87 (in) to 102 (out) : -342.383 Transmembrane helix 6 from 106 (out) to 121 (in) : -988.114 Transmembrane helix 7 from 125 (in) to 140 (out) : -1236.49 Transmembrane helix 8 from 144 (out) to 159 (in) : -874.577 Transmembrane helix 9 from 163 (in) to 178 (out) : -628.71 Transmembrane helix 10 from 182 (out) to 197 (in) : -383.181 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 30 (out) to 45 (in) : -3326.19 Transmembrane helix 2 from 49 (in) to 64 (out) : -1238.64 Transmembrane helix 3 from 68 (out) to 83 (in) : -541.531 Transmembrane helix 4 from 87 (in) to 102 (out) : -342.383 Transmembrane helix 5 from 106 (out) to 121 (in) : -988.114 Transmembrane helix 6 from 125 (in) to 140 (out) : -1236.49 Transmembrane helix 7 from 144 (out) to 159 (in) : -874.577 Transmembrane helix 8 from 163 (in) to 178 (out) : -628.71 Transmembrane helix 9 from 182 (out) to 197 (in) : -383.181 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 49 (in) to 64 (out) : -1238.64 Transmembrane helix 2 from 68 (out) to 83 (in) : -541.531 Transmembrane helix 3 from 87 (in) to 102 (out) : -342.383 Transmembrane helix 4 from 106 (out) to 121 (in) : -988.114 Transmembrane helix 5 from 125 (in) to 140 (out) : -1236.49 Transmembrane helix 6 from 144 (out) to 159 (in) : -874.577 Transmembrane helix 7 from 163 (in) to 178 (out) : -628.71 Transmembrane helix 8 from 182 (out) to 197 (in) : -383.181 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 58 (out) to 73 (in) : -949.876 Transmembrane helix 2 from 77 (in) to 92 (out) : -301.788 Transmembrane helix 3 from 96 (out) to 111 (in) : -544.847 Transmembrane helix 4 from 115 (in) to 130 (out) : -1194.88 Transmembrane helix 5 from 144 (out) to 159 (in) : -874.577 Transmembrane helix 6 from 163 (in) to 178 (out) : -628.71 Transmembrane helix 7 from 182 (out) to 197 (in) : -383.181 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 62 (in) to 77 (out) : -789.276 Transmembrane helix 2 from 81 (out) to 96 (in) : -309.512 Transmembrane helix 3 from 100 (in) to 115 (out) : -660.327 Transmembrane helix 4 from 144 (out) to 159 (in) : -874.577 Transmembrane helix 5 from 163 (in) to 178 (out) : -628.71 Transmembrane helix 6 from 182 (out) to 197 (in) : -383.181 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 73 (out) to 88 (in) : -376.193 Transmembrane helix 2 from 92 (in) to 107 (out) : -413.161 Transmembrane helix 3 from 144 (out) to 159 (in) : -874.577 Transmembrane helix 4 from 163 (in) to 178 (out) : -628.71 Transmembrane helix 5 from 182 (out) to 197 (in) : -383.181 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 73 (in) to 88 (out) : -376.107 Transmembrane helix 2 from 92 (out) to 107 (in) : -421.739 Transmembrane helix 3 from 157 (in) to 172 (out) : -602.024 Transmembrane helix 4 from 182 (out) to 197 (in) : -383.181 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 73 (out) to 88 (in) : -376.193 Transmembrane helix 2 from 92 (in) to 107 (out) : -413.161 Transmembrane helix 3 from 182 (out) to 197 (in) : -383.181 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 78 (in) to 93 (out) : -297.175 Transmembrane helix 2 from 182 (out) to 197 (in) : -383.181 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -25046.2 Transmembrane helix 2 from 30 (in) to 45 (out) : -3329.01 Transmembrane helix 3 from 49 (out) to 64 (in) : -1235.56 Transmembrane helix 4 from 68 (in) to 83 (out) : -529.669 Transmembrane helix 5 from 87 (out) to 102 (in) : -352.517 Transmembrane helix 6 from 106 (in) to 121 (out) : -975.686 Transmembrane helix 7 from 125 (out) to 140 (in) : -1225.71 Transmembrane helix 8 from 144 (in) to 159 (out) : -868.323 Transmembrane helix 9 from 163 (out) to 178 (in) : -629.39 Transmembrane helix 10 from 182 (in) to 197 (out) : -388.019 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 30 (in) to 45 (out) : -3329.01 Transmembrane helix 2 from 49 (out) to 64 (in) : -1235.56 Transmembrane helix 3 from 68 (in) to 83 (out) : -529.669 Transmembrane helix 4 from 87 (out) to 102 (in) : -352.517 Transmembrane helix 5 from 106 (in) to 121 (out) : -975.686 Transmembrane helix 6 from 125 (out) to 140 (in) : -1225.71 Transmembrane helix 7 from 144 (in) to 159 (out) : -868.323 Transmembrane helix 8 from 163 (out) to 178 (in) : -629.39 Transmembrane helix 9 from 182 (in) to 197 (out) : -388.019 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 49 (out) to 64 (in) : -1235.56 Transmembrane helix 2 from 68 (in) to 83 (out) : -529.669 Transmembrane helix 3 from 87 (out) to 102 (in) : -352.517 Transmembrane helix 4 from 106 (in) to 121 (out) : -975.686 Transmembrane helix 5 from 125 (out) to 140 (in) : -1225.71 Transmembrane helix 6 from 144 (in) to 159 (out) : -868.323 Transmembrane helix 7 from 163 (out) to 178 (in) : -629.39 Transmembrane helix 8 from 182 (in) to 197 (out) : -388.019 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 62 (in) to 77 (out) : -789.276 Transmembrane helix 2 from 81 (out) to 96 (in) : -309.512 Transmembrane helix 3 from 100 (in) to 115 (out) : -660.327 Transmembrane helix 4 from 123 (out) to 138 (in) : -1222.33 Transmembrane helix 5 from 144 (in) to 159 (out) : -868.323 Transmembrane helix 6 from 163 (out) to 178 (in) : -629.39 Transmembrane helix 7 from 182 (in) to 197 (out) : -388.019 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 61 (out) to 76 (in) : -835.352 Transmembrane helix 2 from 80 (in) to 95 (out) : -304.64 Transmembrane helix 3 from 99 (out) to 114 (in) : -641.552 Transmembrane helix 4 from 144 (in) to 159 (out) : -868.323 Transmembrane helix 5 from 163 (out) to 178 (in) : -629.39 Transmembrane helix 6 from 182 (in) to 197 (out) : -388.019 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 73 (in) to 88 (out) : -376.107 Transmembrane helix 2 from 92 (out) to 107 (in) : -421.739 Transmembrane helix 3 from 144 (in) to 159 (out) : -868.323 Transmembrane helix 4 from 163 (out) to 178 (in) : -629.39 Transmembrane helix 5 from 182 (in) to 197 (out) : -388.019 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 73 (out) to 88 (in) : -376.193 Transmembrane helix 2 from 92 (in) to 107 (out) : -413.161 Transmembrane helix 3 from 157 (out) to 172 (in) : -592.476 Transmembrane helix 4 from 182 (in) to 197 (out) : -388.019 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 73 (in) to 88 (out) : -376.107 Transmembrane helix 2 from 92 (out) to 107 (in) : -421.739 Transmembrane helix 3 from 182 (in) to 197 (out) : -388.019 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 78 (out) to 93 (in) : -290.225 Transmembrane helix 2 from 182 (in) to 197 (out) : -388.019 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.297175 1 helix (-) : Score = -0.290225 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 Results: Signal peptide: 1-21 Signal score: 82.191 Topology: 78-93 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: -0.290225