BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (83 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P46879 Uncharacterized protein yqgD n=41 Tax=Enterobact...   171   1e-41
UniRef50_B5QY65 Putative inner membrane protein n=10 Tax=Enterob...    82   5e-15

>UniRef50_P46879 Uncharacterized protein yqgD n=41 Tax=Enterobacteriaceae
          RepID=YQGD_ECOLI
          Length = 83

 Score =  171 bits (432), Expect = 1e-41,   Method: Compositional matrix adjust.
 Identities = 83/83 (100%), Positives = 83/83 (100%)

Query: 1  MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTLFPLFFESCLILLKTWLEIFPDN 60
          MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTLFPLFFESCLILLKTWLEIFPDN
Sbjct: 1  MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTLFPLFFESCLILLKTWLEIFPDN 60

Query: 61 AGILRIYLLQFSAIVGYKTRRAA 83
          AGILRIYLLQFSAIVGYKTRRAA
Sbjct: 61 AGILRIYLLQFSAIVGYKTRRAA 83


>UniRef50_B5QY65 Putative inner membrane protein n=10 Tax=Enterobacteriaceae
          RepID=B5QY65_SALEP
          Length = 99

 Score = 82.4 bits (202), Expect = 5e-15,   Method: Compositional matrix adjust.
 Identities = 41/78 (52%), Positives = 50/78 (64%)

Query: 1  MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTLFPLFFESCLILLKTWLEIFPDN 60
          M  D T +L+V +TGSLSVN+YGWINIW AILG FF  FP FFE    + +       DN
Sbjct: 1  MTEDSTMSLKVADTGSLSVNQYGWINIWTAILGHFFAQFPAFFEGRRNVGQNQAINVSDN 60

Query: 61 AGILRIYLLQFSAIVGYK 78
          A I+RIY L F  ++G K
Sbjct: 61 ADIMRIYALLFFCVLGIK 78


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P46879 Uncharacterized protein yqgD n=41 Tax=Enterobact...   131   7e-30
UniRef50_B5QY65 Putative inner membrane protein n=10 Tax=Enterob...   131   7e-30

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P46879 Uncharacterized protein yqgD n=41 Tax=Enterobacteriaceae
          RepID=YQGD_ECOLI
          Length = 83

 Score =  131 bits (330), Expect = 7e-30,   Method: Composition-based stats.
 Identities = 83/83 (100%), Positives = 83/83 (100%)

Query: 1  MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTLFPLFFESCLILLKTWLEIFPDN 60
          MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTLFPLFFESCLILLKTWLEIFPDN
Sbjct: 1  MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTLFPLFFESCLILLKTWLEIFPDN 60

Query: 61 AGILRIYLLQFSAIVGYKTRRAA 83
          AGILRIYLLQFSAIVGYKTRRAA
Sbjct: 61 AGILRIYLLQFSAIVGYKTRRAA 83


>UniRef50_B5QY65 Putative inner membrane protein n=10 Tax=Enterobacteriaceae
          RepID=B5QY65_SALEP
          Length = 99

 Score =  131 bits (329), Expect = 7e-30,   Method: Composition-based stats.
 Identities = 41/78 (52%), Positives = 50/78 (64%)

Query: 1  MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTLFPLFFESCLILLKTWLEIFPDN 60
          M  D T +L+V +TGSLSVN+YGWINIW AILG FF  FP FFE    + +       DN
Sbjct: 1  MTEDSTMSLKVADTGSLSVNQYGWINIWTAILGHFFAQFPAFFEGRRNVGQNQAINVSDN 60

Query: 61 AGILRIYLLQFSAIVGYK 78
          A I+RIY L F  ++G K
Sbjct: 61 ADIMRIYALLFFCVLGIK 78


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.327    0.140    0.447 

Lambda     K      H
   0.267   0.0429    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 365,903,003
Number of Sequences: 3077464
Number of extensions: 12576554
Number of successful extensions: 37729
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 37724
Number of HSP's gapped (non-prelim): 4
length of query: 83
length of database: 1,040,396,356
effective HSP length: 53
effective length of query: 30
effective length of database: 877,290,764
effective search space: 26318722920
effective search space used: 26318722920
T: 11
A: 40
X1: 16 ( 7.6 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 87 (38.1 bits)