BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (44 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P0AD85 PyrBI operon leader peptide n=35 Tax=Enterobacte...    92   7e-18
UniRef50_P0A1W1 PyrBI operon leader peptide n=10 Tax=Enterobacte...    60   2e-08
UniRef50_D2TNX8 PyrBI operon leader peptide n=1 Tax=Citrobacter ...    58   7e-08

>UniRef50_P0AD85 PyrBI operon leader peptide n=35 Tax=Enterobacteriaceae
          RepID=LPPY_ECO57
          Length = 44

 Score = 91.7 bits (226), Expect = 7e-18,   Method: Compositional matrix adjust.
 Identities = 44/44 (100%), Positives = 44/44 (100%)

Query: 1  MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR 44
          MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
Sbjct: 1  MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR 44


>UniRef50_P0A1W1 PyrBI operon leader peptide n=10 Tax=Enterobacteriaceae
          RepID=LPPY_SALTI
          Length = 33

 Score = 60.5 bits (145), Expect = 2e-08,   Method: Compositional matrix adjust.
 Identities = 28/33 (84%), Positives = 31/33 (93%)

Query: 1  MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLN 33
          MVQCVRH VLPRLKKDAGLPFFFPL T+++PLN
Sbjct: 1  MVQCVRHSVLPRLKKDAGLPFFFPLKTNTKPLN 33


>UniRef50_D2TNX8 PyrBI operon leader peptide n=1 Tax=Citrobacter rodentium ICC168
          RepID=D2TNX8_CITRO
          Length = 33

 Score = 58.2 bits (139), Expect = 7e-08,   Method: Compositional matrix adjust.
 Identities = 27/33 (81%), Positives = 30/33 (90%)

Query: 1  MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLN 33
          MVQCVRH VLPRLK DAGLPFFFPL+ +SQPL+
Sbjct: 1  MVQCVRHSVLPRLKADAGLPFFFPLLPYSQPLS 33


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P0AD85 PyrBI operon leader peptide n=35 Tax=Enterobacte...    75   5e-13
UniRef50_P0A1W1 PyrBI operon leader peptide n=10 Tax=Enterobacte...    56   3e-07
UniRef50_D2TNX8 PyrBI operon leader peptide n=1 Tax=Citrobacter ...    54   2e-06

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P0AD85 PyrBI operon leader peptide n=35 Tax=Enterobacteriaceae
          RepID=LPPY_ECO57
          Length = 44

 Score = 75.3 bits (184), Expect = 5e-13,   Method: Composition-based stats.
 Identities = 44/44 (100%), Positives = 44/44 (100%)

Query: 1  MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR 44
          MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
Sbjct: 1  MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR 44


>UniRef50_P0A1W1 PyrBI operon leader peptide n=10 Tax=Enterobacteriaceae
          RepID=LPPY_SALTI
          Length = 33

 Score = 56.5 bits (135), Expect = 3e-07,   Method: Composition-based stats.
 Identities = 28/33 (84%), Positives = 31/33 (93%)

Query: 1  MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLN 33
          MVQCVRH VLPRLKKDAGLPFFFPL T+++PLN
Sbjct: 1  MVQCVRHSVLPRLKKDAGLPFFFPLKTNTKPLN 33


>UniRef50_D2TNX8 PyrBI operon leader peptide n=1 Tax=Citrobacter rodentium ICC168
          RepID=D2TNX8_CITRO
          Length = 33

 Score = 53.8 bits (128), Expect = 2e-06,   Method: Composition-based stats.
 Identities = 27/33 (81%), Positives = 30/33 (90%)

Query: 1  MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLN 33
          MVQCVRH VLPRLK DAGLPFFFPL+ +SQPL+
Sbjct: 1  MVQCVRHSVLPRLKADAGLPFFFPLLPYSQPLS 33


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.321    0.148    0.508 

Lambda     K      H
   0.267   0.0450    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 226,267,138
Number of Sequences: 3077464
Number of extensions: 6336867
Number of successful extensions: 19481
Number of sequences better than 1.0e-01: 3
Number of HSP's better than  0.1 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 19475
Number of HSP's gapped (non-prelim): 6
length of query: 44
length of database: 1,040,396,356
effective HSP length: 18
effective length of query: 26
effective length of database: 985,002,004
effective search space: 25610052104
effective search space used: 25610052104
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (20.8 bits)
S2: 87 (38.0 bits)