BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (81 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P0ACV8 Uncharacterized protein ymjA n=72 Tax=Enterobact...   167   1e-40
UniRef50_C9Y5F1 Uncharacterized protein ymjA n=3 Tax=Enterobacte...   130   1e-29

>UniRef50_P0ACV8 Uncharacterized protein ymjA n=72 Tax=Enterobacteriaceae
          RepID=YMJA_ECOLI
          Length = 81

 Score =  167 bits (422), Expect = 1e-40,   Method: Compositional matrix adjust.
 Identities = 81/81 (100%), Positives = 81/81 (100%)

Query: 1  MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLMNNEEISEEAQHEMAAE 60
          MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLMNNEEISEEAQHEMAAE
Sbjct: 1  MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLMNNEEISEEAQHEMAAE 60

Query: 61 AGINPVRIDEIAEFLNQWGNE 81
          AGINPVRIDEIAEFLNQWGNE
Sbjct: 61 AGINPVRIDEIAEFLNQWGNE 81


>UniRef50_C9Y5F1 Uncharacterized protein ymjA n=3 Tax=Enterobacteriaceae
          RepID=C9Y5F1_CROTZ
          Length = 81

 Score =  130 bits (328), Expect = 1e-29,   Method: Compositional matrix adjust.
 Identities = 62/81 (76%), Positives = 72/81 (88%)

Query: 1  MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLMNNEEISEEAQHEMAAE 60
          M++DIP+K++DIADEYATE A PV++A+R  LAHYFQLL+TRLMNNEEISEEAQHEMA+E
Sbjct: 1  MSNDIPIKFYDIADEYATETALPVSDADRDRLAHYFQLLITRLMNNEEISEEAQHEMASE 60

Query: 61 AGINPVRIDEIAEFLNQWGNE 81
          AGI    IDEIA FLNQWGNE
Sbjct: 61 AGIAEAHIDEIANFLNQWGNE 81


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P0ACV8 Uncharacterized protein ymjA n=72 Tax=Enterobact...   135   4e-31
UniRef50_C9Y5F1 Uncharacterized protein ymjA n=3 Tax=Enterobacte...   133   2e-30

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P0ACV8 Uncharacterized protein ymjA n=72 Tax=Enterobacteriaceae
          RepID=YMJA_ECOLI
          Length = 81

 Score =  135 bits (340), Expect = 4e-31,   Method: Composition-based stats.
 Identities = 81/81 (100%), Positives = 81/81 (100%)

Query: 1  MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLMNNEEISEEAQHEMAAE 60
          MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLMNNEEISEEAQHEMAAE
Sbjct: 1  MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLMNNEEISEEAQHEMAAE 60

Query: 61 AGINPVRIDEIAEFLNQWGNE 81
          AGINPVRIDEIAEFLNQWGNE
Sbjct: 61 AGINPVRIDEIAEFLNQWGNE 81


>UniRef50_C9Y5F1 Uncharacterized protein ymjA n=3 Tax=Enterobacteriaceae
          RepID=C9Y5F1_CROTZ
          Length = 81

 Score =  133 bits (334), Expect = 2e-30,   Method: Composition-based stats.
 Identities = 62/81 (76%), Positives = 72/81 (88%)

Query: 1  MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLMNNEEISEEAQHEMAAE 60
          M++DIP+K++DIADEYATE A PV++A+R  LAHYFQLL+TRLMNNEEISEEAQHEMA+E
Sbjct: 1  MSNDIPIKFYDIADEYATETALPVSDADRDRLAHYFQLLITRLMNNEEISEEAQHEMASE 60

Query: 61 AGINPVRIDEIAEFLNQWGNE 81
          AGI    IDEIA FLNQWGNE
Sbjct: 61 AGIAEAHIDEIANFLNQWGNE 81


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.312    0.137    0.392 

Lambda     K      H
   0.267   0.0397    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 292,925,182
Number of Sequences: 3077464
Number of extensions: 8874876
Number of successful extensions: 27926
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 27922
Number of HSP's gapped (non-prelim): 4
length of query: 81
length of database: 1,040,396,356
effective HSP length: 52
effective length of query: 29
effective length of database: 880,368,228
effective search space: 25530678612
effective search space used: 25530678612
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.3 bits)
S2: 87 (38.2 bits)