BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (110 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P77609 Protein flxA n=9 Tax=Escherichia RepID=FLXA_ECOLI     217   8e-56
UniRef50_C8Q492 Putative uncharacterized protein n=1 Tax=Pantoea...    38   0.100

>UniRef50_P77609 Protein flxA n=9 Tax=Escherichia RepID=FLXA_ECOLI
          Length = 110

 Score =  217 bits (553), Expect = 8e-56,   Method: Compositional matrix adjust.
 Identities = 110/110 (100%), Positives = 110/110 (100%)

Query: 1   MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISSEEGMTTQQKKEMAALV 60
           MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISSEEGMTTQQKKEMAALV
Sbjct: 1   MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISSEEGMTTQQKKEMAALV 60

Query: 61  QKQIESLWAQLEQLLRQQAEKKNEDATVQPDKKEEKKDDTNTAGTIDIYV 110
           QKQIESLWAQLEQLLRQQAEKKNEDATVQPDKKEEKKDDTNTAGTIDIYV
Sbjct: 61  QKQIESLWAQLEQLLRQQAEKKNEDATVQPDKKEEKKDDTNTAGTIDIYV 110


>UniRef50_C8Q492 Putative uncharacterized protein n=1 Tax=Pantoea sp. At-9b
          RepID=C8Q492_9ENTR
          Length = 117

 Score = 37.7 bits (86), Expect = 0.100,   Method: Compositional matrix adjust.
 Identities = 19/51 (37%), Positives = 36/51 (70%)

Query: 24 EIAKITRQIQVLTEKLGKISSEEGMTTQQKKEMAALVQKQIESLWAQLEQL 74
          +IA + +QIQ LT++L  +SS++ +T +QK E   L++ QI+ + +Q+ Q+
Sbjct: 22 QIASLNKQIQSLTKELKDLSSDDTLTDEQKSEQEQLLESQIQMIESQIAQI 72


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P77609 Protein flxA n=9 Tax=Escherichia RepID=FLXA_ECOLI     161   5e-39

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P77609 Protein flxA n=9 Tax=Escherichia RepID=FLXA_ECOLI
          Length = 110

 Score =  161 bits (408), Expect = 5e-39,   Method: Composition-based stats.
 Identities = 110/110 (100%), Positives = 110/110 (100%)

Query: 1   MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISSEEGMTTQQKKEMAALV 60
           MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISSEEGMTTQQKKEMAALV
Sbjct: 1   MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISSEEGMTTQQKKEMAALV 60

Query: 61  QKQIESLWAQLEQLLRQQAEKKNEDATVQPDKKEEKKDDTNTAGTIDIYV 110
           QKQIESLWAQLEQLLRQQAEKKNEDATVQPDKKEEKKDDTNTAGTIDIYV
Sbjct: 61  QKQIESLWAQLEQLLRQQAEKKNEDATVQPDKKEEKKDDTNTAGTIDIYV 110


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.302    0.118    0.298 

Lambda     K      H
   0.267   0.0384    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 339,417,129
Number of Sequences: 3077464
Number of extensions: 10274145
Number of successful extensions: 78958
Number of sequences better than 1.0e-01: 17
Number of HSP's better than  0.1 without gapping: 9
Number of HSP's successfully gapped in prelim test: 33
Number of HSP's that attempted gapping in prelim test: 78889
Number of HSP's gapped (non-prelim): 77
length of query: 110
length of database: 1,040,396,356
effective HSP length: 78
effective length of query: 32
effective length of database: 800,354,164
effective search space: 25611333248
effective search space used: 25611333248
T: 11
A: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (21.8 bits)
S2: 87 (38.2 bits)