Processing 1 helix: Transmembrane helix 1 from 430 (in) to 445 (out) : 37.957 Score = 0.032889 Processing 1 helix: Transmembrane helix 1 from 429 (out) to 444 (in) : 30.782 Score = 0.036424 Processing 24 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -747.544 Transmembrane helix 2 from 23 (out) to 38 (in) : -254.327 Transmembrane helix 3 from 42 (in) to 57 (out) : -467.975 Transmembrane helix 4 from 61 (out) to 76 (in) : -695.1 Transmembrane helix 5 from 80 (in) to 95 (out) : -955.481 Transmembrane helix 6 from 99 (out) to 114 (in) : -704.992 Transmembrane helix 7 from 124 (in) to 139 (out) : -831.366 Transmembrane helix 8 from 143 (out) to 158 (in) : -272.822 Transmembrane helix 9 from 162 (in) to 177 (out) : -447.194 Transmembrane helix 10 from 181 (out) to 196 (in) : -1040.19 Transmembrane helix 11 from 200 (in) to 215 (out) : -635.677 Transmembrane helix 12 from 219 (out) to 234 (in) : -518.793 Transmembrane helix 13 from 238 (in) to 253 (out) : -775.833 Transmembrane helix 14 from 257 (out) to 272 (in) : -27.18 Transmembrane helix 15 from 276 (in) to 291 (out) : -626.077 Transmembrane helix 16 from 295 (out) to 310 (in) : -613.462 Transmembrane helix 17 from 314 (in) to 329 (out) : -705.011 Transmembrane helix 18 from 333 (out) to 348 (in) : -640.68 Transmembrane helix 19 from 352 (in) to 367 (out) : -705.21 Transmembrane helix 20 from 371 (out) to 386 (in) : -470.501 Transmembrane helix 21 from 390 (in) to 405 (out) : -794.675 Transmembrane helix 22 from 409 (out) to 424 (in) : -656.32 Transmembrane helix 23 from 428 (in) to 443 (out) : 27.549 Transmembrane helix 24 from 447 (out) to 462 (in) : -259.9 Score = -100000.000000 Processing 23 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -745.956 Transmembrane helix 2 from 23 (in) to 38 (out) : -260.273 Transmembrane helix 3 from 42 (out) to 57 (in) : -474.425 Transmembrane helix 4 from 61 (in) to 76 (out) : -691.4 Transmembrane helix 5 from 80 (out) to 95 (in) : -951.619 Transmembrane helix 6 from 99 (in) to 114 (out) : -684.808 Transmembrane helix 7 from 135 (out) to 150 (in) : -216.383 Transmembrane helix 8 from 154 (in) to 169 (out) : -322.686 Transmembrane helix 9 from 173 (out) to 188 (in) : -845.902 Transmembrane helix 10 from 199 (in) to 214 (out) : -693.545 Transmembrane helix 11 from 218 (out) to 233 (in) : -472.799 Transmembrane helix 12 from 237 (in) to 252 (out) : -819.18 Transmembrane helix 13 from 256 (out) to 271 (in) : -34.367 Transmembrane helix 14 from 275 (in) to 290 (out) : -577.984 Transmembrane helix 15 from 294 (out) to 309 (in) : -638.688 Transmembrane helix 16 from 313 (in) to 328 (out) : -672.217 Transmembrane helix 17 from 332 (out) to 347 (in) : -644.103 Transmembrane helix 18 from 351 (in) to 366 (out) : -697.071 Transmembrane helix 19 from 370 (out) to 385 (in) : -508.08 Transmembrane helix 20 from 389 (in) to 404 (out) : -745.874 Transmembrane helix 21 from 409 (out) to 424 (in) : -656.32 Transmembrane helix 22 from 428 (in) to 443 (out) : 27.549 Transmembrane helix 23 from 447 (out) to 462 (in) : -259.9 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 5 (in) to 20 (out) : -708.543 Transmembrane helix 2 from 24 (out) to 39 (in) : -239.669 Transmembrane helix 3 from 43 (in) to 58 (out) : -509.673 Transmembrane helix 4 from 62 (out) to 77 (in) : -680.034 Transmembrane helix 5 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 6 from 135 (out) to 150 (in) : -216.383 Transmembrane helix 7 from 154 (in) to 169 (out) : -322.686 Transmembrane helix 8 from 173 (out) to 188 (in) : -845.902 Transmembrane helix 9 from 199 (in) to 214 (out) : -693.545 Transmembrane helix 10 from 218 (out) to 233 (in) : -472.799 Transmembrane helix 11 from 237 (in) to 252 (out) : -819.18 Transmembrane helix 12 from 256 (out) to 271 (in) : -34.367 Transmembrane helix 13 from 275 (in) to 290 (out) : -577.984 Transmembrane helix 14 from 294 (out) to 309 (in) : -638.688 Transmembrane helix 15 from 313 (in) to 328 (out) : -672.217 Transmembrane helix 16 from 332 (out) to 347 (in) : -644.103 Transmembrane helix 17 from 351 (in) to 366 (out) : -697.071 Transmembrane helix 18 from 370 (out) to 385 (in) : -508.08 Transmembrane helix 19 from 389 (in) to 404 (out) : -745.874 Transmembrane helix 20 from 409 (out) to 424 (in) : -656.32 Transmembrane helix 21 from 428 (in) to 443 (out) : 27.549 Transmembrane helix 22 from 447 (out) to 462 (in) : -259.9 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 5 (out) to 20 (in) : -707.257 Transmembrane helix 2 from 24 (in) to 39 (out) : -246.031 Transmembrane helix 3 from 43 (out) to 58 (in) : -515.927 Transmembrane helix 4 from 62 (in) to 77 (out) : -674.566 Transmembrane helix 5 from 94 (out) to 109 (in) : -529.398 Transmembrane helix 6 from 135 (in) to 150 (out) : -211.917 Transmembrane helix 7 from 154 (out) to 169 (in) : -336.114 Transmembrane helix 8 from 173 (in) to 188 (out) : -844.998 Transmembrane helix 9 from 201 (out) to 216 (in) : -573.138 Transmembrane helix 10 from 220 (in) to 235 (out) : -579.864 Transmembrane helix 11 from 246 (out) to 261 (in) : -436.538 Transmembrane helix 12 from 265 (in) to 280 (out) : -30.055 Transmembrane helix 13 from 286 (out) to 301 (in) : -722.019 Transmembrane helix 14 from 305 (in) to 320 (out) : -549.023 Transmembrane helix 15 from 324 (out) to 339 (in) : -772.917 Transmembrane helix 16 from 343 (in) to 358 (out) : -598.033 Transmembrane helix 17 from 362 (out) to 377 (in) : -739.77 Transmembrane helix 18 from 381 (in) to 396 (out) : -335.632 Transmembrane helix 19 from 409 (out) to 424 (in) : -656.32 Transmembrane helix 20 from 428 (in) to 443 (out) : 27.549 Transmembrane helix 21 from 447 (out) to 462 (in) : -259.9 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 5 (in) to 20 (out) : -708.543 Transmembrane helix 2 from 24 (out) to 39 (in) : -239.669 Transmembrane helix 3 from 43 (in) to 58 (out) : -509.673 Transmembrane helix 4 from 62 (out) to 77 (in) : -680.034 Transmembrane helix 5 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 6 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 7 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 8 from 201 (out) to 216 (in) : -573.138 Transmembrane helix 9 from 220 (in) to 235 (out) : -579.864 Transmembrane helix 10 from 246 (out) to 261 (in) : -436.538 Transmembrane helix 11 from 265 (in) to 280 (out) : -30.055 Transmembrane helix 12 from 286 (out) to 301 (in) : -722.019 Transmembrane helix 13 from 305 (in) to 320 (out) : -549.023 Transmembrane helix 14 from 324 (out) to 339 (in) : -772.917 Transmembrane helix 15 from 343 (in) to 358 (out) : -598.033 Transmembrane helix 16 from 362 (out) to 377 (in) : -739.77 Transmembrane helix 17 from 381 (in) to 396 (out) : -335.632 Transmembrane helix 18 from 409 (out) to 424 (in) : -656.32 Transmembrane helix 19 from 428 (in) to 443 (out) : 27.549 Transmembrane helix 20 from 447 (out) to 462 (in) : -259.9 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -348.777 Transmembrane helix 2 from 38 (in) to 53 (out) : -284.276 Transmembrane helix 3 from 62 (out) to 77 (in) : -680.034 Transmembrane helix 4 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 5 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 6 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 7 from 201 (out) to 216 (in) : -573.138 Transmembrane helix 8 from 220 (in) to 235 (out) : -579.864 Transmembrane helix 9 from 246 (out) to 261 (in) : -436.538 Transmembrane helix 10 from 265 (in) to 280 (out) : -30.055 Transmembrane helix 11 from 286 (out) to 301 (in) : -722.019 Transmembrane helix 12 from 305 (in) to 320 (out) : -549.023 Transmembrane helix 13 from 324 (out) to 339 (in) : -772.917 Transmembrane helix 14 from 343 (in) to 358 (out) : -598.033 Transmembrane helix 15 from 362 (out) to 377 (in) : -739.77 Transmembrane helix 16 from 381 (in) to 396 (out) : -335.632 Transmembrane helix 17 from 409 (out) to 424 (in) : -656.32 Transmembrane helix 18 from 428 (in) to 443 (out) : 27.549 Transmembrane helix 19 from 447 (out) to 462 (in) : -259.9 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 5 (in) to 20 (out) : -708.543 Transmembrane helix 2 from 24 (out) to 39 (in) : -239.669 Transmembrane helix 3 from 43 (in) to 58 (out) : -509.673 Transmembrane helix 4 from 62 (out) to 77 (in) : -680.034 Transmembrane helix 5 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 6 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 7 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 8 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 9 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 10 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 11 from 290 (in) to 305 (out) : -704.586 Transmembrane helix 12 from 309 (out) to 324 (in) : -531.285 Transmembrane helix 13 from 335 (in) to 350 (out) : -608.178 Transmembrane helix 14 from 354 (out) to 369 (in) : -706.39 Transmembrane helix 15 from 379 (in) to 394 (out) : -307.49 Transmembrane helix 16 from 409 (out) to 424 (in) : -656.32 Transmembrane helix 17 from 428 (in) to 443 (out) : 27.549 Transmembrane helix 18 from 447 (out) to 462 (in) : -259.9 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -348.777 Transmembrane helix 2 from 38 (in) to 53 (out) : -284.276 Transmembrane helix 3 from 62 (out) to 77 (in) : -680.034 Transmembrane helix 4 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 5 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 6 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 7 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 8 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 9 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 10 from 290 (in) to 305 (out) : -704.586 Transmembrane helix 11 from 309 (out) to 324 (in) : -531.285 Transmembrane helix 12 from 335 (in) to 350 (out) : -608.178 Transmembrane helix 13 from 354 (out) to 369 (in) : -706.39 Transmembrane helix 14 from 379 (in) to 394 (out) : -307.49 Transmembrane helix 15 from 409 (out) to 424 (in) : -656.32 Transmembrane helix 16 from 428 (in) to 443 (out) : 27.549 Transmembrane helix 17 from 447 (out) to 462 (in) : -259.9 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 5 (in) to 20 (out) : -708.543 Transmembrane helix 2 from 24 (out) to 39 (in) : -239.669 Transmembrane helix 3 from 43 (in) to 58 (out) : -509.673 Transmembrane helix 4 from 62 (out) to 77 (in) : -680.034 Transmembrane helix 5 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 6 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 7 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 8 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 9 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 10 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 11 from 290 (in) to 305 (out) : -704.586 Transmembrane helix 12 from 309 (out) to 324 (in) : -531.285 Transmembrane helix 13 from 342 (in) to 357 (out) : -584.972 Transmembrane helix 14 from 379 (out) to 394 (in) : -288.61 Transmembrane helix 15 from 423 (in) to 438 (out) : -39.493 Transmembrane helix 16 from 442 (out) to 457 (in) : -125.369 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -348.777 Transmembrane helix 2 from 38 (in) to 53 (out) : -284.276 Transmembrane helix 3 from 62 (out) to 77 (in) : -680.034 Transmembrane helix 4 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 5 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 6 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 7 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 8 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 9 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 10 from 290 (in) to 305 (out) : -704.586 Transmembrane helix 11 from 309 (out) to 324 (in) : -531.285 Transmembrane helix 12 from 342 (in) to 357 (out) : -584.972 Transmembrane helix 13 from 379 (out) to 394 (in) : -288.61 Transmembrane helix 14 from 423 (in) to 438 (out) : -39.493 Transmembrane helix 15 from 442 (out) to 457 (in) : -125.369 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : -355.623 Transmembrane helix 2 from 38 (out) to 53 (in) : -288.324 Transmembrane helix 3 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 4 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 5 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 6 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 7 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 8 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 9 from 290 (in) to 305 (out) : -704.586 Transmembrane helix 10 from 309 (out) to 324 (in) : -531.285 Transmembrane helix 11 from 342 (in) to 357 (out) : -584.972 Transmembrane helix 12 from 379 (out) to 394 (in) : -288.61 Transmembrane helix 13 from 423 (in) to 438 (out) : -39.493 Transmembrane helix 14 from 442 (out) to 457 (in) : -125.369 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -348.777 Transmembrane helix 2 from 38 (in) to 53 (out) : -284.276 Transmembrane helix 3 from 62 (out) to 77 (in) : -680.034 Transmembrane helix 4 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 5 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 6 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 7 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 8 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 9 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 10 from 309 (in) to 324 (out) : -525.115 Transmembrane helix 11 from 379 (out) to 394 (in) : -288.61 Transmembrane helix 12 from 423 (in) to 438 (out) : -39.493 Transmembrane helix 13 from 442 (out) to 457 (in) : -125.369 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : -355.623 Transmembrane helix 2 from 38 (out) to 53 (in) : -288.324 Transmembrane helix 3 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 4 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 5 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 6 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 7 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 8 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 9 from 309 (in) to 324 (out) : -525.115 Transmembrane helix 10 from 379 (out) to 394 (in) : -288.61 Transmembrane helix 11 from 423 (in) to 438 (out) : -39.493 Transmembrane helix 12 from 442 (out) to 457 (in) : -125.369 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -348.777 Transmembrane helix 2 from 38 (in) to 53 (out) : -284.276 Transmembrane helix 3 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 4 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 5 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 6 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 7 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 8 from 309 (in) to 324 (out) : -525.115 Transmembrane helix 9 from 379 (out) to 394 (in) : -288.61 Transmembrane helix 10 from 423 (in) to 438 (out) : -39.493 Transmembrane helix 11 from 442 (out) to 457 (in) : -125.369 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : -355.623 Transmembrane helix 2 from 38 (out) to 53 (in) : -288.324 Transmembrane helix 3 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 4 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 5 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 6 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 7 from 261 (in) to 276 (out) : 30.407 Transmembrane helix 8 from 379 (out) to 394 (in) : -288.61 Transmembrane helix 9 from 423 (in) to 438 (out) : -39.493 Transmembrane helix 10 from 442 (out) to 457 (in) : -125.369 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -348.777 Transmembrane helix 2 from 38 (in) to 53 (out) : -284.276 Transmembrane helix 3 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 4 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 5 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 6 from 261 (in) to 276 (out) : 30.407 Transmembrane helix 7 from 379 (out) to 394 (in) : -288.61 Transmembrane helix 8 from 423 (in) to 438 (out) : -39.493 Transmembrane helix 9 from 442 (out) to 457 (in) : -125.369 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 27 (in) to 42 (out) : -223.928 Transmembrane helix 2 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 3 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 4 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 5 from 261 (in) to 276 (out) : 30.407 Transmembrane helix 6 from 379 (out) to 394 (in) : -288.61 Transmembrane helix 7 from 423 (in) to 438 (out) : -39.493 Transmembrane helix 8 from 442 (out) to 457 (in) : -125.369 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 27 (out) to 42 (in) : -219.572 Transmembrane helix 2 from 137 (in) to 152 (out) : -189.449 Transmembrane helix 3 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 4 from 261 (in) to 276 (out) : 30.407 Transmembrane helix 5 from 379 (out) to 394 (in) : -288.61 Transmembrane helix 6 from 423 (in) to 438 (out) : -39.493 Transmembrane helix 7 from 442 (out) to 457 (in) : -125.369 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 27 (in) to 42 (out) : -223.928 Transmembrane helix 2 from 137 (out) to 152 (in) : -196.351 Transmembrane helix 3 from 261 (in) to 276 (out) : 30.407 Transmembrane helix 4 from 379 (out) to 394 (in) : -288.61 Transmembrane helix 5 from 423 (in) to 438 (out) : -39.493 Transmembrane helix 6 from 442 (out) to 457 (in) : -125.369 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 27 (out) to 42 (in) : -219.572 Transmembrane helix 2 from 137 (in) to 152 (out) : -189.449 Transmembrane helix 3 from 261 (out) to 276 (in) : 35.093 Transmembrane helix 4 from 423 (in) to 438 (out) : -39.493 Transmembrane helix 5 from 442 (out) to 457 (in) : -125.369 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 137 (in) to 152 (out) : -189.449 Transmembrane helix 2 from 261 (out) to 276 (in) : 35.093 Transmembrane helix 3 from 423 (in) to 438 (out) : -39.493 Transmembrane helix 4 from 442 (out) to 457 (in) : -125.369 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 137 (out) to 152 (in) : -196.351 Transmembrane helix 2 from 261 (in) to 276 (out) : 30.407 Transmembrane helix 3 from 429 (out) to 444 (in) : 30.782 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 261 (in) to 276 (out) : 30.407 Transmembrane helix 2 from 429 (out) to 444 (in) : 30.782 Score = -100000.000000 Processing 24 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -745.956 Transmembrane helix 2 from 23 (in) to 38 (out) : -260.273 Transmembrane helix 3 from 42 (out) to 57 (in) : -474.425 Transmembrane helix 4 from 61 (in) to 76 (out) : -691.4 Transmembrane helix 5 from 80 (out) to 95 (in) : -951.619 Transmembrane helix 6 from 99 (in) to 114 (out) : -684.808 Transmembrane helix 7 from 124 (out) to 139 (in) : -821.534 Transmembrane helix 8 from 143 (in) to 158 (out) : -255.578 Transmembrane helix 9 from 162 (out) to 177 (in) : -437.306 Transmembrane helix 10 from 181 (in) to 196 (out) : -1042.6 Transmembrane helix 11 from 200 (out) to 215 (in) : -626.223 Transmembrane helix 12 from 219 (in) to 234 (out) : -526.907 Transmembrane helix 13 from 238 (out) to 253 (in) : -788.867 Transmembrane helix 14 from 257 (in) to 272 (out) : -30.92 Transmembrane helix 15 from 276 (out) to 291 (in) : -630.023 Transmembrane helix 16 from 295 (in) to 310 (out) : -623.938 Transmembrane helix 17 from 314 (out) to 329 (in) : -705.489 Transmembrane helix 18 from 333 (in) to 348 (out) : -631.32 Transmembrane helix 19 from 352 (out) to 367 (in) : -691.99 Transmembrane helix 20 from 371 (in) to 386 (out) : -470.599 Transmembrane helix 21 from 390 (out) to 405 (in) : -794.925 Transmembrane helix 22 from 409 (in) to 424 (out) : -651.08 Transmembrane helix 23 from 428 (out) to 443 (in) : 27.751 Transmembrane helix 24 from 447 (in) to 462 (out) : -245.6 Score = -100000.000000 Processing 23 helices: Transmembrane helix 1 from 5 (in) to 20 (out) : -708.543 Transmembrane helix 2 from 24 (out) to 39 (in) : -239.669 Transmembrane helix 3 from 43 (in) to 58 (out) : -509.673 Transmembrane helix 4 from 62 (out) to 77 (in) : -680.034 Transmembrane helix 5 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 6 from 124 (out) to 139 (in) : -821.534 Transmembrane helix 7 from 143 (in) to 158 (out) : -255.578 Transmembrane helix 8 from 162 (out) to 177 (in) : -437.306 Transmembrane helix 9 from 181 (in) to 196 (out) : -1042.6 Transmembrane helix 10 from 200 (out) to 215 (in) : -626.223 Transmembrane helix 11 from 219 (in) to 234 (out) : -526.907 Transmembrane helix 12 from 238 (out) to 253 (in) : -788.867 Transmembrane helix 13 from 257 (in) to 272 (out) : -30.92 Transmembrane helix 14 from 276 (out) to 291 (in) : -630.023 Transmembrane helix 15 from 295 (in) to 310 (out) : -623.938 Transmembrane helix 16 from 314 (out) to 329 (in) : -705.489 Transmembrane helix 17 from 333 (in) to 348 (out) : -631.32 Transmembrane helix 18 from 352 (out) to 367 (in) : -691.99 Transmembrane helix 19 from 371 (in) to 386 (out) : -470.599 Transmembrane helix 20 from 390 (out) to 405 (in) : -794.925 Transmembrane helix 21 from 409 (in) to 424 (out) : -651.08 Transmembrane helix 22 from 428 (out) to 443 (in) : 27.751 Transmembrane helix 23 from 447 (in) to 462 (out) : -245.6 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -745.956 Transmembrane helix 2 from 23 (in) to 38 (out) : -260.273 Transmembrane helix 3 from 42 (out) to 57 (in) : -474.425 Transmembrane helix 4 from 61 (in) to 76 (out) : -691.4 Transmembrane helix 5 from 80 (out) to 95 (in) : -951.619 Transmembrane helix 6 from 99 (in) to 114 (out) : -684.808 Transmembrane helix 7 from 135 (out) to 150 (in) : -216.383 Transmembrane helix 8 from 154 (in) to 169 (out) : -322.686 Transmembrane helix 9 from 173 (out) to 188 (in) : -845.902 Transmembrane helix 10 from 200 (in) to 215 (out) : -635.677 Transmembrane helix 11 from 219 (out) to 234 (in) : -518.793 Transmembrane helix 12 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 13 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 14 from 286 (in) to 301 (out) : -725.881 Transmembrane helix 15 from 305 (out) to 320 (in) : -556.977 Transmembrane helix 16 from 324 (in) to 339 (out) : -767.983 Transmembrane helix 17 from 343 (out) to 358 (in) : -596.267 Transmembrane helix 18 from 362 (in) to 377 (out) : -733.53 Transmembrane helix 19 from 381 (out) to 396 (in) : -315.668 Transmembrane helix 20 from 409 (in) to 424 (out) : -651.08 Transmembrane helix 21 from 428 (out) to 443 (in) : 27.751 Transmembrane helix 22 from 447 (in) to 462 (out) : -245.6 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 5 (in) to 20 (out) : -708.543 Transmembrane helix 2 from 24 (out) to 39 (in) : -239.669 Transmembrane helix 3 from 43 (in) to 58 (out) : -509.673 Transmembrane helix 4 from 62 (out) to 77 (in) : -680.034 Transmembrane helix 5 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 6 from 135 (out) to 150 (in) : -216.383 Transmembrane helix 7 from 154 (in) to 169 (out) : -322.686 Transmembrane helix 8 from 173 (out) to 188 (in) : -845.902 Transmembrane helix 9 from 200 (in) to 215 (out) : -635.677 Transmembrane helix 10 from 219 (out) to 234 (in) : -518.793 Transmembrane helix 11 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 12 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 13 from 286 (in) to 301 (out) : -725.881 Transmembrane helix 14 from 305 (out) to 320 (in) : -556.977 Transmembrane helix 15 from 324 (in) to 339 (out) : -767.983 Transmembrane helix 16 from 343 (out) to 358 (in) : -596.267 Transmembrane helix 17 from 362 (in) to 377 (out) : -733.53 Transmembrane helix 18 from 381 (out) to 396 (in) : -315.668 Transmembrane helix 19 from 409 (in) to 424 (out) : -651.08 Transmembrane helix 20 from 428 (out) to 443 (in) : 27.751 Transmembrane helix 21 from 447 (in) to 462 (out) : -245.6 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -348.777 Transmembrane helix 2 from 38 (in) to 53 (out) : -284.276 Transmembrane helix 3 from 62 (out) to 77 (in) : -680.034 Transmembrane helix 4 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 5 from 135 (out) to 150 (in) : -216.383 Transmembrane helix 6 from 154 (in) to 169 (out) : -322.686 Transmembrane helix 7 from 173 (out) to 188 (in) : -845.902 Transmembrane helix 8 from 200 (in) to 215 (out) : -635.677 Transmembrane helix 9 from 219 (out) to 234 (in) : -518.793 Transmembrane helix 10 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 11 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 12 from 286 (in) to 301 (out) : -725.881 Transmembrane helix 13 from 305 (out) to 320 (in) : -556.977 Transmembrane helix 14 from 324 (in) to 339 (out) : -767.983 Transmembrane helix 15 from 343 (out) to 358 (in) : -596.267 Transmembrane helix 16 from 362 (in) to 377 (out) : -733.53 Transmembrane helix 17 from 381 (out) to 396 (in) : -315.668 Transmembrane helix 18 from 409 (in) to 424 (out) : -651.08 Transmembrane helix 19 from 428 (out) to 443 (in) : 27.751 Transmembrane helix 20 from 447 (in) to 462 (out) : -245.6 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 5 (in) to 20 (out) : -708.543 Transmembrane helix 2 from 24 (out) to 39 (in) : -239.669 Transmembrane helix 3 from 43 (in) to 58 (out) : -509.673 Transmembrane helix 4 from 62 (out) to 77 (in) : -680.034 Transmembrane helix 5 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 6 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 7 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 8 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 9 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 10 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 11 from 286 (in) to 301 (out) : -725.881 Transmembrane helix 12 from 305 (out) to 320 (in) : -556.977 Transmembrane helix 13 from 324 (in) to 339 (out) : -767.983 Transmembrane helix 14 from 343 (out) to 358 (in) : -596.267 Transmembrane helix 15 from 362 (in) to 377 (out) : -733.53 Transmembrane helix 16 from 381 (out) to 396 (in) : -315.668 Transmembrane helix 17 from 409 (in) to 424 (out) : -651.08 Transmembrane helix 18 from 428 (out) to 443 (in) : 27.751 Transmembrane helix 19 from 447 (in) to 462 (out) : -245.6 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -348.777 Transmembrane helix 2 from 38 (in) to 53 (out) : -284.276 Transmembrane helix 3 from 62 (out) to 77 (in) : -680.034 Transmembrane helix 4 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 5 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 6 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 7 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 8 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 9 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 10 from 286 (in) to 301 (out) : -725.881 Transmembrane helix 11 from 305 (out) to 320 (in) : -556.977 Transmembrane helix 12 from 324 (in) to 339 (out) : -767.983 Transmembrane helix 13 from 343 (out) to 358 (in) : -596.267 Transmembrane helix 14 from 362 (in) to 377 (out) : -733.53 Transmembrane helix 15 from 381 (out) to 396 (in) : -315.668 Transmembrane helix 16 from 409 (in) to 424 (out) : -651.08 Transmembrane helix 17 from 428 (out) to 443 (in) : 27.751 Transmembrane helix 18 from 447 (in) to 462 (out) : -245.6 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 5 (in) to 20 (out) : -708.543 Transmembrane helix 2 from 24 (out) to 39 (in) : -239.669 Transmembrane helix 3 from 43 (in) to 58 (out) : -509.673 Transmembrane helix 4 from 62 (out) to 77 (in) : -680.034 Transmembrane helix 5 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 6 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 7 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 8 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 9 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 10 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 11 from 290 (in) to 305 (out) : -704.586 Transmembrane helix 12 from 309 (out) to 324 (in) : -531.285 Transmembrane helix 13 from 342 (in) to 357 (out) : -584.972 Transmembrane helix 14 from 379 (out) to 394 (in) : -288.61 Transmembrane helix 15 from 409 (in) to 424 (out) : -651.08 Transmembrane helix 16 from 428 (out) to 443 (in) : 27.751 Transmembrane helix 17 from 447 (in) to 462 (out) : -245.6 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -348.777 Transmembrane helix 2 from 38 (in) to 53 (out) : -284.276 Transmembrane helix 3 from 62 (out) to 77 (in) : -680.034 Transmembrane helix 4 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 5 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 6 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 7 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 8 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 9 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 10 from 290 (in) to 305 (out) : -704.586 Transmembrane helix 11 from 309 (out) to 324 (in) : -531.285 Transmembrane helix 12 from 342 (in) to 357 (out) : -584.972 Transmembrane helix 13 from 379 (out) to 394 (in) : -288.61 Transmembrane helix 14 from 409 (in) to 424 (out) : -651.08 Transmembrane helix 15 from 428 (out) to 443 (in) : 27.751 Transmembrane helix 16 from 447 (in) to 462 (out) : -245.6 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : -355.623 Transmembrane helix 2 from 38 (out) to 53 (in) : -288.324 Transmembrane helix 3 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 4 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 5 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 6 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 7 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 8 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 9 from 290 (in) to 305 (out) : -704.586 Transmembrane helix 10 from 309 (out) to 324 (in) : -531.285 Transmembrane helix 11 from 342 (in) to 357 (out) : -584.972 Transmembrane helix 12 from 379 (out) to 394 (in) : -288.61 Transmembrane helix 13 from 409 (in) to 424 (out) : -651.08 Transmembrane helix 14 from 428 (out) to 443 (in) : 27.751 Transmembrane helix 15 from 447 (in) to 462 (out) : -245.6 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -348.777 Transmembrane helix 2 from 38 (in) to 53 (out) : -284.276 Transmembrane helix 3 from 62 (out) to 77 (in) : -680.034 Transmembrane helix 4 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 5 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 6 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 7 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 8 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 9 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 10 from 309 (in) to 324 (out) : -525.115 Transmembrane helix 11 from 342 (out) to 357 (in) : -585.428 Transmembrane helix 12 from 379 (in) to 394 (out) : -307.49 Transmembrane helix 13 from 423 (out) to 438 (in) : -26.607 Transmembrane helix 14 from 442 (in) to 457 (out) : -118.131 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : -355.623 Transmembrane helix 2 from 38 (out) to 53 (in) : -288.324 Transmembrane helix 3 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 4 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 5 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 6 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 7 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 8 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 9 from 309 (in) to 324 (out) : -525.115 Transmembrane helix 10 from 342 (out) to 357 (in) : -585.428 Transmembrane helix 11 from 379 (in) to 394 (out) : -307.49 Transmembrane helix 12 from 423 (out) to 438 (in) : -26.607 Transmembrane helix 13 from 442 (in) to 457 (out) : -118.131 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -348.777 Transmembrane helix 2 from 38 (in) to 53 (out) : -284.276 Transmembrane helix 3 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 4 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 5 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 6 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 7 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 8 from 309 (in) to 324 (out) : -525.115 Transmembrane helix 9 from 342 (out) to 357 (in) : -585.428 Transmembrane helix 10 from 379 (in) to 394 (out) : -307.49 Transmembrane helix 11 from 423 (out) to 438 (in) : -26.607 Transmembrane helix 12 from 442 (in) to 457 (out) : -118.131 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 19 (in) to 34 (out) : -355.623 Transmembrane helix 2 from 38 (out) to 53 (in) : -288.324 Transmembrane helix 3 from 94 (in) to 109 (out) : -508.102 Transmembrane helix 4 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 5 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 6 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 7 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 8 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 9 from 379 (in) to 394 (out) : -307.49 Transmembrane helix 10 from 423 (out) to 438 (in) : -26.607 Transmembrane helix 11 from 442 (in) to 457 (out) : -118.131 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -348.777 Transmembrane helix 2 from 38 (in) to 53 (out) : -284.276 Transmembrane helix 3 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 4 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 5 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 6 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 7 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 8 from 379 (in) to 394 (out) : -307.49 Transmembrane helix 9 from 423 (out) to 438 (in) : -26.607 Transmembrane helix 10 from 442 (in) to 457 (out) : -118.131 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 27 (in) to 42 (out) : -223.928 Transmembrane helix 2 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 3 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 4 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 5 from 246 (in) to 261 (out) : -436.362 Transmembrane helix 6 from 265 (out) to 280 (in) : -27.045 Transmembrane helix 7 from 379 (in) to 394 (out) : -307.49 Transmembrane helix 8 from 423 (out) to 438 (in) : -26.607 Transmembrane helix 9 from 442 (in) to 457 (out) : -118.131 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 19 (out) to 34 (in) : -348.777 Transmembrane helix 2 from 38 (in) to 53 (out) : -284.276 Transmembrane helix 3 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 4 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 5 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 6 from 261 (in) to 276 (out) : 30.407 Transmembrane helix 7 from 423 (out) to 438 (in) : -26.607 Transmembrane helix 8 from 442 (in) to 457 (out) : -118.131 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 27 (in) to 42 (out) : -223.928 Transmembrane helix 2 from 136 (out) to 151 (in) : -199.225 Transmembrane helix 3 from 155 (in) to 170 (out) : -321.345 Transmembrane helix 4 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 5 from 261 (in) to 276 (out) : 30.407 Transmembrane helix 6 from 423 (out) to 438 (in) : -26.607 Transmembrane helix 7 from 442 (in) to 457 (out) : -118.131 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 27 (out) to 42 (in) : -219.572 Transmembrane helix 2 from 137 (in) to 152 (out) : -189.449 Transmembrane helix 3 from 211 (out) to 226 (in) : -302.703 Transmembrane helix 4 from 261 (in) to 276 (out) : 30.407 Transmembrane helix 5 from 423 (out) to 438 (in) : -26.607 Transmembrane helix 6 from 442 (in) to 457 (out) : -118.131 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 27 (in) to 42 (out) : -223.928 Transmembrane helix 2 from 137 (out) to 152 (in) : -196.351 Transmembrane helix 3 from 261 (in) to 276 (out) : 30.407 Transmembrane helix 4 from 423 (out) to 438 (in) : -26.607 Transmembrane helix 5 from 442 (in) to 457 (out) : -118.131 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 137 (out) to 152 (in) : -196.351 Transmembrane helix 2 from 261 (in) to 276 (out) : 30.407 Transmembrane helix 3 from 423 (out) to 438 (in) : -26.607 Transmembrane helix 4 from 442 (in) to 457 (out) : -118.131 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 261 (in) to 276 (out) : 30.407 Transmembrane helix 2 from 423 (out) to 438 (in) : -26.607 Transmembrane helix 3 from 442 (in) to 457 (out) : -118.131 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 261 (out) to 276 (in) : 35.093 Transmembrane helix 2 from 430 (in) to 445 (out) : 37.957 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.032889 1 helix (-) : Score = 0.036424 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 22 helices (+) : Score = -100000 22 helices (-) : Score = -100000 23 helices (+) : Score = -100000 23 helices (-) : Score = -100000 24 helices (+) : Score = -100000 24 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 429-444 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.036424