Processing 1 helix: Transmembrane helix 1 from 217 (in) to 232 (out) : -23 Score = -0.023000 Processing 1 helix: Transmembrane helix 1 from 217 (out) to 232 (in) : -23 Score = -0.023000 Processing 16 helices: Transmembrane helix 1 from 7 (in) to 22 (out) : -13618.1 Transmembrane helix 2 from 26 (out) to 41 (in) : -311.3 Transmembrane helix 3 from 45 (in) to 60 (out) : -784.7 Transmembrane helix 4 from 64 (out) to 79 (in) : -588.5 Transmembrane helix 5 from 83 (in) to 98 (out) : -152.75 Transmembrane helix 6 from 102 (out) to 117 (in) : -403.2 Transmembrane helix 7 from 121 (in) to 136 (out) : -681.95 Transmembrane helix 8 from 140 (out) to 155 (in) : -293.9 Transmembrane helix 9 from 159 (in) to 174 (out) : -483.35 Transmembrane helix 10 from 178 (out) to 193 (in) : -771.7 Transmembrane helix 11 from 197 (in) to 212 (out) : -533.25 Transmembrane helix 12 from 216 (out) to 231 (in) : -31.05 Transmembrane helix 13 from 235 (in) to 250 (out) : -289.65 Transmembrane helix 14 from 254 (out) to 269 (in) : -244.2 Transmembrane helix 15 from 273 (in) to 288 (out) : -113.5 Transmembrane helix 16 from 292 (out) to 307 (in) : -904.15 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -246.329 Transmembrane helix 2 from 37 (in) to 52 (out) : -469.8 Transmembrane helix 3 from 64 (out) to 79 (in) : -588.5 Transmembrane helix 4 from 83 (in) to 98 (out) : -152.75 Transmembrane helix 5 from 102 (out) to 117 (in) : -403.2 Transmembrane helix 6 from 121 (in) to 136 (out) : -681.95 Transmembrane helix 7 from 140 (out) to 155 (in) : -293.9 Transmembrane helix 8 from 159 (in) to 174 (out) : -483.35 Transmembrane helix 9 from 178 (out) to 193 (in) : -771.7 Transmembrane helix 10 from 197 (in) to 212 (out) : -533.25 Transmembrane helix 11 from 216 (out) to 231 (in) : -31.05 Transmembrane helix 12 from 235 (in) to 250 (out) : -289.65 Transmembrane helix 13 from 254 (out) to 269 (in) : -244.2 Transmembrane helix 14 from 273 (in) to 288 (out) : -113.5 Transmembrane helix 15 from 292 (out) to 307 (in) : -904.15 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -233.871 Transmembrane helix 2 from 37 (out) to 52 (in) : -469.8 Transmembrane helix 3 from 69 (in) to 84 (out) : -317.6 Transmembrane helix 4 from 88 (out) to 103 (in) : -228.6 Transmembrane helix 5 from 107 (in) to 122 (out) : -558.8 Transmembrane helix 6 from 132 (out) to 147 (in) : -270.3 Transmembrane helix 7 from 151 (in) to 166 (out) : -453.25 Transmembrane helix 8 from 170 (out) to 185 (in) : -634.75 Transmembrane helix 9 from 197 (in) to 212 (out) : -533.25 Transmembrane helix 10 from 216 (out) to 231 (in) : -31.05 Transmembrane helix 11 from 235 (in) to 250 (out) : -289.65 Transmembrane helix 12 from 254 (out) to 269 (in) : -244.2 Transmembrane helix 13 from 273 (in) to 288 (out) : -113.5 Transmembrane helix 14 from 292 (out) to 307 (in) : -904.15 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -246.329 Transmembrane helix 2 from 37 (in) to 52 (out) : -469.8 Transmembrane helix 3 from 69 (out) to 84 (in) : -317.6 Transmembrane helix 4 from 88 (in) to 103 (out) : -228.6 Transmembrane helix 5 from 107 (out) to 122 (in) : -558.8 Transmembrane helix 6 from 132 (in) to 147 (out) : -270.3 Transmembrane helix 7 from 151 (out) to 166 (in) : -453.25 Transmembrane helix 8 from 170 (in) to 185 (out) : -634.75 Transmembrane helix 9 from 197 (out) to 212 (in) : -533.25 Transmembrane helix 10 from 216 (in) to 231 (out) : -31.05 Transmembrane helix 11 from 235 (out) to 250 (in) : -289.65 Transmembrane helix 12 from 254 (in) to 269 (out) : -244.2 Transmembrane helix 13 from 273 (out) to 288 (in) : -113.5 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -233.871 Transmembrane helix 2 from 37 (out) to 52 (in) : -469.8 Transmembrane helix 3 from 78 (in) to 93 (out) : -101.5 Transmembrane helix 4 from 97 (out) to 112 (in) : -287.6 Transmembrane helix 5 from 132 (in) to 147 (out) : -270.3 Transmembrane helix 6 from 151 (out) to 166 (in) : -453.25 Transmembrane helix 7 from 170 (in) to 185 (out) : -634.75 Transmembrane helix 8 from 197 (out) to 212 (in) : -533.25 Transmembrane helix 9 from 216 (in) to 231 (out) : -31.05 Transmembrane helix 10 from 235 (out) to 250 (in) : -289.65 Transmembrane helix 11 from 254 (in) to 269 (out) : -244.2 Transmembrane helix 12 from 273 (out) to 288 (in) : -113.5 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -246.329 Transmembrane helix 2 from 37 (in) to 52 (out) : -469.8 Transmembrane helix 3 from 78 (out) to 93 (in) : -101.5 Transmembrane helix 4 from 97 (in) to 112 (out) : -287.6 Transmembrane helix 5 from 132 (out) to 147 (in) : -270.3 Transmembrane helix 6 from 151 (in) to 166 (out) : -453.25 Transmembrane helix 7 from 197 (out) to 212 (in) : -533.25 Transmembrane helix 8 from 216 (in) to 231 (out) : -31.05 Transmembrane helix 9 from 235 (out) to 250 (in) : -289.65 Transmembrane helix 10 from 254 (in) to 269 (out) : -244.2 Transmembrane helix 11 from 273 (out) to 288 (in) : -113.5 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -233.871 Transmembrane helix 2 from 37 (out) to 52 (in) : -469.8 Transmembrane helix 3 from 78 (in) to 93 (out) : -101.5 Transmembrane helix 4 from 97 (out) to 112 (in) : -287.6 Transmembrane helix 5 from 132 (in) to 147 (out) : -270.3 Transmembrane helix 6 from 151 (out) to 166 (in) : -453.25 Transmembrane helix 7 from 216 (in) to 231 (out) : -31.05 Transmembrane helix 8 from 235 (out) to 250 (in) : -289.65 Transmembrane helix 9 from 254 (in) to 269 (out) : -244.2 Transmembrane helix 10 from 273 (out) to 288 (in) : -113.5 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -246.329 Transmembrane helix 2 from 37 (in) to 52 (out) : -469.8 Transmembrane helix 3 from 78 (out) to 93 (in) : -101.5 Transmembrane helix 4 from 97 (in) to 112 (out) : -287.6 Transmembrane helix 5 from 136 (out) to 151 (in) : -253.5 Transmembrane helix 6 from 216 (in) to 231 (out) : -31.05 Transmembrane helix 7 from 235 (out) to 250 (in) : -289.65 Transmembrane helix 8 from 254 (in) to 269 (out) : -244.2 Transmembrane helix 9 from 273 (out) to 288 (in) : -113.5 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -233.871 Transmembrane helix 2 from 78 (out) to 93 (in) : -101.5 Transmembrane helix 3 from 97 (in) to 112 (out) : -287.6 Transmembrane helix 4 from 136 (out) to 151 (in) : -253.5 Transmembrane helix 5 from 216 (in) to 231 (out) : -31.05 Transmembrane helix 6 from 235 (out) to 250 (in) : -289.65 Transmembrane helix 7 from 254 (in) to 269 (out) : -244.2 Transmembrane helix 8 from 273 (out) to 288 (in) : -113.5 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -246.329 Transmembrane helix 2 from 78 (in) to 93 (out) : -101.5 Transmembrane helix 3 from 97 (out) to 112 (in) : -287.6 Transmembrane helix 4 from 136 (in) to 151 (out) : -253.5 Transmembrane helix 5 from 217 (out) to 232 (in) : -23 Transmembrane helix 6 from 245 (in) to 260 (out) : -210.7 Transmembrane helix 7 from 267 (out) to 282 (in) : -59.65 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -233.871 Transmembrane helix 2 from 78 (out) to 93 (in) : -101.5 Transmembrane helix 3 from 136 (in) to 151 (out) : -253.5 Transmembrane helix 4 from 217 (out) to 232 (in) : -23 Transmembrane helix 5 from 245 (in) to 260 (out) : -210.7 Transmembrane helix 6 from 267 (out) to 282 (in) : -59.65 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -246.329 Transmembrane helix 2 from 78 (in) to 93 (out) : -101.5 Transmembrane helix 3 from 217 (out) to 232 (in) : -23 Transmembrane helix 4 from 245 (in) to 260 (out) : -210.7 Transmembrane helix 5 from 267 (out) to 282 (in) : -59.65 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 78 (in) to 93 (out) : -101.5 Transmembrane helix 2 from 217 (out) to 232 (in) : -23 Transmembrane helix 3 from 245 (in) to 260 (out) : -210.7 Transmembrane helix 4 from 267 (out) to 282 (in) : -59.65 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 78 (out) to 93 (in) : -101.5 Transmembrane helix 2 from 217 (in) to 232 (out) : -23 Transmembrane helix 3 from 267 (out) to 282 (in) : -59.65 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 217 (in) to 232 (out) : -23 Transmembrane helix 2 from 267 (out) to 282 (in) : -59.65 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : -13566.5 Transmembrane helix 2 from 26 (in) to 41 (out) : -303.9 Transmembrane helix 3 from 45 (out) to 60 (in) : -784.7 Transmembrane helix 4 from 64 (in) to 79 (out) : -588.5 Transmembrane helix 5 from 83 (out) to 98 (in) : -152.75 Transmembrane helix 6 from 102 (in) to 117 (out) : -403.2 Transmembrane helix 7 from 121 (out) to 136 (in) : -681.95 Transmembrane helix 8 from 140 (in) to 155 (out) : -293.9 Transmembrane helix 9 from 159 (out) to 174 (in) : -483.35 Transmembrane helix 10 from 178 (in) to 193 (out) : -771.7 Transmembrane helix 11 from 197 (out) to 212 (in) : -533.25 Transmembrane helix 12 from 216 (in) to 231 (out) : -31.05 Transmembrane helix 13 from 235 (out) to 250 (in) : -289.65 Transmembrane helix 14 from 254 (in) to 269 (out) : -244.2 Transmembrane helix 15 from 273 (out) to 288 (in) : -113.5 Transmembrane helix 16 from 292 (in) to 307 (out) : -904.15 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -233.871 Transmembrane helix 2 from 37 (out) to 52 (in) : -469.8 Transmembrane helix 3 from 64 (in) to 79 (out) : -588.5 Transmembrane helix 4 from 83 (out) to 98 (in) : -152.75 Transmembrane helix 5 from 102 (in) to 117 (out) : -403.2 Transmembrane helix 6 from 121 (out) to 136 (in) : -681.95 Transmembrane helix 7 from 140 (in) to 155 (out) : -293.9 Transmembrane helix 8 from 159 (out) to 174 (in) : -483.35 Transmembrane helix 9 from 178 (in) to 193 (out) : -771.7 Transmembrane helix 10 from 197 (out) to 212 (in) : -533.25 Transmembrane helix 11 from 216 (in) to 231 (out) : -31.05 Transmembrane helix 12 from 235 (out) to 250 (in) : -289.65 Transmembrane helix 13 from 254 (in) to 269 (out) : -244.2 Transmembrane helix 14 from 273 (out) to 288 (in) : -113.5 Transmembrane helix 15 from 292 (in) to 307 (out) : -904.15 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -246.329 Transmembrane helix 2 from 37 (in) to 52 (out) : -469.8 Transmembrane helix 3 from 69 (out) to 84 (in) : -317.6 Transmembrane helix 4 from 88 (in) to 103 (out) : -228.6 Transmembrane helix 5 from 107 (out) to 122 (in) : -558.8 Transmembrane helix 6 from 132 (in) to 147 (out) : -270.3 Transmembrane helix 7 from 151 (out) to 166 (in) : -453.25 Transmembrane helix 8 from 170 (in) to 185 (out) : -634.75 Transmembrane helix 9 from 197 (out) to 212 (in) : -533.25 Transmembrane helix 10 from 216 (in) to 231 (out) : -31.05 Transmembrane helix 11 from 235 (out) to 250 (in) : -289.65 Transmembrane helix 12 from 254 (in) to 269 (out) : -244.2 Transmembrane helix 13 from 273 (out) to 288 (in) : -113.5 Transmembrane helix 14 from 292 (in) to 307 (out) : -904.15 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -233.871 Transmembrane helix 2 from 37 (out) to 52 (in) : -469.8 Transmembrane helix 3 from 69 (in) to 84 (out) : -317.6 Transmembrane helix 4 from 88 (out) to 103 (in) : -228.6 Transmembrane helix 5 from 107 (in) to 122 (out) : -558.8 Transmembrane helix 6 from 132 (out) to 147 (in) : -270.3 Transmembrane helix 7 from 151 (in) to 166 (out) : -453.25 Transmembrane helix 8 from 170 (out) to 185 (in) : -634.75 Transmembrane helix 9 from 197 (in) to 212 (out) : -533.25 Transmembrane helix 10 from 216 (out) to 231 (in) : -31.05 Transmembrane helix 11 from 235 (in) to 250 (out) : -289.65 Transmembrane helix 12 from 254 (out) to 269 (in) : -244.2 Transmembrane helix 13 from 273 (in) to 288 (out) : -113.5 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -246.329 Transmembrane helix 2 from 37 (in) to 52 (out) : -469.8 Transmembrane helix 3 from 78 (out) to 93 (in) : -101.5 Transmembrane helix 4 from 97 (in) to 112 (out) : -287.6 Transmembrane helix 5 from 132 (out) to 147 (in) : -270.3 Transmembrane helix 6 from 151 (in) to 166 (out) : -453.25 Transmembrane helix 7 from 170 (out) to 185 (in) : -634.75 Transmembrane helix 8 from 197 (in) to 212 (out) : -533.25 Transmembrane helix 9 from 216 (out) to 231 (in) : -31.05 Transmembrane helix 10 from 235 (in) to 250 (out) : -289.65 Transmembrane helix 11 from 254 (out) to 269 (in) : -244.2 Transmembrane helix 12 from 273 (in) to 288 (out) : -113.5 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -233.871 Transmembrane helix 2 from 37 (out) to 52 (in) : -469.8 Transmembrane helix 3 from 78 (in) to 93 (out) : -101.5 Transmembrane helix 4 from 97 (out) to 112 (in) : -287.6 Transmembrane helix 5 from 132 (in) to 147 (out) : -270.3 Transmembrane helix 6 from 151 (out) to 166 (in) : -453.25 Transmembrane helix 7 from 197 (in) to 212 (out) : -533.25 Transmembrane helix 8 from 216 (out) to 231 (in) : -31.05 Transmembrane helix 9 from 235 (in) to 250 (out) : -289.65 Transmembrane helix 10 from 254 (out) to 269 (in) : -244.2 Transmembrane helix 11 from 273 (in) to 288 (out) : -113.5 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -246.329 Transmembrane helix 2 from 37 (in) to 52 (out) : -469.8 Transmembrane helix 3 from 78 (out) to 93 (in) : -101.5 Transmembrane helix 4 from 97 (in) to 112 (out) : -287.6 Transmembrane helix 5 from 132 (out) to 147 (in) : -270.3 Transmembrane helix 6 from 151 (in) to 166 (out) : -453.25 Transmembrane helix 7 from 216 (out) to 231 (in) : -31.05 Transmembrane helix 8 from 235 (in) to 250 (out) : -289.65 Transmembrane helix 9 from 254 (out) to 269 (in) : -244.2 Transmembrane helix 10 from 273 (in) to 288 (out) : -113.5 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -233.871 Transmembrane helix 2 from 37 (out) to 52 (in) : -469.8 Transmembrane helix 3 from 78 (in) to 93 (out) : -101.5 Transmembrane helix 4 from 97 (out) to 112 (in) : -287.6 Transmembrane helix 5 from 136 (in) to 151 (out) : -253.5 Transmembrane helix 6 from 216 (out) to 231 (in) : -31.05 Transmembrane helix 7 from 235 (in) to 250 (out) : -289.65 Transmembrane helix 8 from 254 (out) to 269 (in) : -244.2 Transmembrane helix 9 from 273 (in) to 288 (out) : -113.5 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -246.329 Transmembrane helix 2 from 78 (in) to 93 (out) : -101.5 Transmembrane helix 3 from 97 (out) to 112 (in) : -287.6 Transmembrane helix 4 from 136 (in) to 151 (out) : -253.5 Transmembrane helix 5 from 216 (out) to 231 (in) : -31.05 Transmembrane helix 6 from 235 (in) to 250 (out) : -289.65 Transmembrane helix 7 from 254 (out) to 269 (in) : -244.2 Transmembrane helix 8 from 273 (in) to 288 (out) : -113.5 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -233.871 Transmembrane helix 2 from 78 (out) to 93 (in) : -101.5 Transmembrane helix 3 from 97 (in) to 112 (out) : -287.6 Transmembrane helix 4 from 136 (out) to 151 (in) : -253.5 Transmembrane helix 5 from 217 (in) to 232 (out) : -23 Transmembrane helix 6 from 245 (out) to 260 (in) : -210.7 Transmembrane helix 7 from 267 (in) to 282 (out) : -59.65 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -246.329 Transmembrane helix 2 from 78 (in) to 93 (out) : -101.5 Transmembrane helix 3 from 136 (out) to 151 (in) : -253.5 Transmembrane helix 4 from 217 (in) to 232 (out) : -23 Transmembrane helix 5 from 245 (out) to 260 (in) : -210.7 Transmembrane helix 6 from 267 (in) to 282 (out) : -59.65 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 78 (in) to 93 (out) : -101.5 Transmembrane helix 2 from 136 (out) to 151 (in) : -253.5 Transmembrane helix 3 from 217 (in) to 232 (out) : -23 Transmembrane helix 4 from 245 (out) to 260 (in) : -210.7 Transmembrane helix 5 from 267 (in) to 282 (out) : -59.65 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 78 (out) to 93 (in) : -101.5 Transmembrane helix 2 from 217 (in) to 232 (out) : -23 Transmembrane helix 3 from 245 (out) to 260 (in) : -210.7 Transmembrane helix 4 from 267 (in) to 282 (out) : -59.65 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 78 (in) to 93 (out) : -101.5 Transmembrane helix 2 from 217 (out) to 232 (in) : -23 Transmembrane helix 3 from 267 (in) to 282 (out) : -59.65 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 217 (out) to 232 (in) : -23 Transmembrane helix 2 from 267 (in) to 282 (out) : -59.65 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.023 1 helix (-) : Score = -0.023 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 Results: Signal peptide: 1-15 Signal score: 41.407 Topology: 217-232 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: -0.023