Processing 1 helix: Transmembrane helix 1 from 8 (in) to 26 (out) : 3397.32 Score = 3.397295 Processing 1 helix: Transmembrane helix 1 from 8 (out) to 26 (in) : 3389.28 Score = 3.389305 Processing 9 helices: Transmembrane helix 1 from 8 (in) to 25 (out) : 3361.83 Transmembrane helix 2 from 29 (out) to 44 (in) : -1093.75 Transmembrane helix 3 from 48 (in) to 63 (out) : -499.769 Transmembrane helix 4 from 67 (out) to 82 (in) : -781.04 Transmembrane helix 5 from 86 (in) to 101 (out) : -840.229 Transmembrane helix 6 from 105 (out) to 120 (in) : -639.153 Transmembrane helix 7 from 124 (in) to 139 (out) : -1237.58 Transmembrane helix 8 from 143 (out) to 158 (in) : -1046.79 Transmembrane helix 9 from 164 (in) to 179 (out) : -1463.77 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 8 (out) to 25 (in) : 3355.97 Transmembrane helix 2 from 29 (in) to 44 (out) : -1079.34 Transmembrane helix 3 from 48 (out) to 63 (in) : -503.331 Transmembrane helix 4 from 67 (in) to 82 (out) : -787.46 Transmembrane helix 5 from 86 (out) to 101 (in) : -842.871 Transmembrane helix 6 from 105 (in) to 120 (out) : -640.047 Transmembrane helix 7 from 124 (out) to 139 (in) : -1232.72 Transmembrane helix 8 from 143 (in) to 158 (out) : -1049.01 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 8 (in) to 25 (out) : 3361.83 Transmembrane helix 2 from 29 (out) to 44 (in) : -1093.75 Transmembrane helix 3 from 48 (in) to 63 (out) : -499.769 Transmembrane helix 4 from 67 (out) to 82 (in) : -781.04 Transmembrane helix 5 from 86 (in) to 101 (out) : -840.229 Transmembrane helix 6 from 105 (out) to 120 (in) : -639.153 Transmembrane helix 7 from 135 (in) to 150 (out) : -826.815 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 8 (out) to 26 (in) : 3389.28 Transmembrane helix 2 from 42 (in) to 57 (out) : -578.561 Transmembrane helix 3 from 61 (out) to 76 (in) : -646.116 Transmembrane helix 4 from 80 (in) to 95 (out) : -1047.56 Transmembrane helix 5 from 99 (out) to 114 (in) : -342.35 Transmembrane helix 6 from 135 (in) to 150 (out) : -826.815 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 8 (in) to 26 (out) : 3397.32 Transmembrane helix 2 from 43 (out) to 58 (in) : -549.913 Transmembrane helix 3 from 62 (in) to 77 (out) : -671.93 Transmembrane helix 4 from 98 (out) to 113 (in) : -332.838 Transmembrane helix 5 from 135 (in) to 150 (out) : -826.815 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 8 (out) to 26 (in) : 3389.28 Transmembrane helix 2 from 43 (in) to 58 (out) : -552.687 Transmembrane helix 3 from 62 (out) to 77 (in) : -670.37 Transmembrane helix 4 from 98 (in) to 113 (out) : -320.662 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 8 (in) to 26 (out) : 3397.32 Transmembrane helix 2 from 49 (out) to 64 (in) : -497.977 Transmembrane helix 3 from 98 (in) to 113 (out) : -320.662 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 8 (out) to 26 (in) : 3389.28 Transmembrane helix 2 from 98 (in) to 113 (out) : -320.662 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 8 (out) to 25 (in) : 3355.97 Transmembrane helix 2 from 29 (in) to 44 (out) : -1079.34 Transmembrane helix 3 from 48 (out) to 63 (in) : -503.331 Transmembrane helix 4 from 67 (in) to 82 (out) : -787.46 Transmembrane helix 5 from 86 (out) to 101 (in) : -842.871 Transmembrane helix 6 from 105 (in) to 120 (out) : -640.047 Transmembrane helix 7 from 124 (out) to 139 (in) : -1232.72 Transmembrane helix 8 from 143 (in) to 158 (out) : -1049.01 Transmembrane helix 9 from 164 (out) to 179 (in) : -1440.13 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 8 (in) to 25 (out) : 3361.83 Transmembrane helix 2 from 29 (out) to 44 (in) : -1093.75 Transmembrane helix 3 from 48 (in) to 63 (out) : -499.769 Transmembrane helix 4 from 67 (out) to 82 (in) : -781.04 Transmembrane helix 5 from 86 (in) to 101 (out) : -840.229 Transmembrane helix 6 from 105 (out) to 120 (in) : -639.153 Transmembrane helix 7 from 135 (in) to 150 (out) : -826.815 Transmembrane helix 8 from 164 (out) to 179 (in) : -1440.13 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 8 (out) to 25 (in) : 3355.97 Transmembrane helix 2 from 29 (in) to 44 (out) : -1079.34 Transmembrane helix 3 from 48 (out) to 63 (in) : -503.331 Transmembrane helix 4 from 67 (in) to 82 (out) : -787.46 Transmembrane helix 5 from 86 (out) to 101 (in) : -842.871 Transmembrane helix 6 from 105 (in) to 120 (out) : -640.047 Transmembrane helix 7 from 135 (out) to 150 (in) : -833.085 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 8 (in) to 26 (out) : 3397.32 Transmembrane helix 2 from 42 (out) to 57 (in) : -573.739 Transmembrane helix 3 from 61 (in) to 76 (out) : -650.284 Transmembrane helix 4 from 80 (out) to 95 (in) : -1044.24 Transmembrane helix 5 from 99 (in) to 114 (out) : -330.85 Transmembrane helix 6 from 135 (out) to 150 (in) : -833.085 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 8 (out) to 26 (in) : 3389.28 Transmembrane helix 2 from 43 (in) to 58 (out) : -552.687 Transmembrane helix 3 from 62 (out) to 77 (in) : -670.37 Transmembrane helix 4 from 97 (in) to 112 (out) : -322.119 Transmembrane helix 5 from 135 (out) to 150 (in) : -833.085 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 8 (in) to 26 (out) : 3397.32 Transmembrane helix 2 from 43 (out) to 58 (in) : -549.913 Transmembrane helix 3 from 62 (in) to 77 (out) : -671.93 Transmembrane helix 4 from 98 (out) to 113 (in) : -332.838 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 8 (out) to 26 (in) : 3389.28 Transmembrane helix 2 from 49 (in) to 64 (out) : -494.423 Transmembrane helix 3 from 98 (out) to 113 (in) : -332.838 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 8 (in) to 26 (out) : 3397.32 Transmembrane helix 2 from 98 (out) to 113 (in) : -332.838 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 3.39729 1 helix (-) : Score = 3.38931 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 8-26 Re-entrant helices: Not detected. Helix count: 1 N-terminal: in Score: 3.39729