Processing 1 helix: Transmembrane helix 1 from 171 (in) to 191 (out) : 19.825 Score = 0.019825 Processing 1 helix: Transmembrane helix 1 from 37 (out) to 57 (in) : 31.739 Score = 0.040438 Processing 22 helices: Transmembrane helix 1 from 6 (in) to 21 (out) : -365.261 Transmembrane helix 2 from 33 (out) to 48 (in) : -7.43 Transmembrane helix 3 from 52 (in) to 67 (out) : 3.57 Transmembrane helix 4 from 71 (out) to 86 (in) : 1.819 Transmembrane helix 5 from 90 (in) to 105 (out) : 3.531 Transmembrane helix 6 from 109 (out) to 124 (in) : 6.429 Transmembrane helix 7 from 128 (in) to 143 (out) : -3.286 Transmembrane helix 8 from 147 (out) to 162 (in) : -1.015 Transmembrane helix 9 from 166 (in) to 181 (out) : 0.0869999 Transmembrane helix 10 from 185 (out) to 200 (in) : 5.553 Transmembrane helix 11 from 204 (in) to 219 (out) : -1.871 Transmembrane helix 12 from 223 (out) to 238 (in) : 0 Transmembrane helix 13 from 242 (in) to 257 (out) : 0 Transmembrane helix 14 from 261 (out) to 276 (in) : 0 Transmembrane helix 15 from 280 (in) to 295 (out) : 0 Transmembrane helix 16 from 299 (out) to 314 (in) : 0 Transmembrane helix 17 from 318 (in) to 333 (out) : 0 Transmembrane helix 18 from 337 (out) to 352 (in) : 0 Transmembrane helix 19 from 356 (in) to 371 (out) : 0 Transmembrane helix 20 from 375 (out) to 390 (in) : 0 Transmembrane helix 21 from 394 (in) to 409 (out) : 0 Transmembrane helix 22 from 413 (out) to 428 (in) : 0 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 33 (out) to 48 (in) : -7.43 Transmembrane helix 2 from 52 (in) to 67 (out) : 3.57 Transmembrane helix 3 from 71 (out) to 86 (in) : 1.819 Transmembrane helix 4 from 90 (in) to 105 (out) : 3.531 Transmembrane helix 5 from 109 (out) to 124 (in) : 6.429 Transmembrane helix 6 from 128 (in) to 143 (out) : -3.286 Transmembrane helix 7 from 147 (out) to 162 (in) : -1.015 Transmembrane helix 8 from 166 (in) to 181 (out) : 0.0869999 Transmembrane helix 9 from 185 (out) to 200 (in) : 5.553 Transmembrane helix 10 from 204 (in) to 219 (out) : -1.871 Transmembrane helix 11 from 223 (out) to 238 (in) : 0 Transmembrane helix 12 from 242 (in) to 257 (out) : 0 Transmembrane helix 13 from 261 (out) to 276 (in) : 0 Transmembrane helix 14 from 280 (in) to 295 (out) : 0 Transmembrane helix 15 from 299 (out) to 314 (in) : 0 Transmembrane helix 16 from 318 (in) to 333 (out) : 0 Transmembrane helix 17 from 337 (out) to 352 (in) : 0 Transmembrane helix 18 from 356 (in) to 371 (out) : 0 Transmembrane helix 19 from 375 (out) to 390 (in) : 0 Transmembrane helix 20 from 394 (in) to 409 (out) : 0 Transmembrane helix 21 from 413 (out) to 428 (in) : 0 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 37 (in) to 64 (out) : 23.678 Transmembrane helix 2 from 68 (out) to 83 (in) : 1.723 Transmembrane helix 3 from 87 (in) to 102 (out) : 4.405 Transmembrane helix 4 from 106 (out) to 121 (in) : 5.318 Transmembrane helix 5 from 125 (in) to 140 (out) : -3.868 Transmembrane helix 6 from 144 (out) to 159 (in) : 1.646 Transmembrane helix 7 from 166 (in) to 181 (out) : 0.0869999 Transmembrane helix 8 from 185 (out) to 200 (in) : 5.553 Transmembrane helix 9 from 204 (in) to 219 (out) : -1.871 Transmembrane helix 10 from 223 (out) to 238 (in) : 0 Transmembrane helix 11 from 242 (in) to 257 (out) : 0 Transmembrane helix 12 from 261 (out) to 276 (in) : 0 Transmembrane helix 13 from 280 (in) to 295 (out) : 0 Transmembrane helix 14 from 299 (out) to 314 (in) : 0 Transmembrane helix 15 from 318 (in) to 333 (out) : 0 Transmembrane helix 16 from 337 (out) to 352 (in) : 0 Transmembrane helix 17 from 356 (in) to 371 (out) : 0 Transmembrane helix 18 from 375 (out) to 390 (in) : 0 Transmembrane helix 19 from 394 (in) to 409 (out) : 0 Transmembrane helix 20 from 413 (out) to 428 (in) : 0 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 37 (out) to 52 (in) : 28.171 Transmembrane helix 2 from 56 (in) to 71 (out) : 4.459 Transmembrane helix 3 from 75 (out) to 90 (in) : 3.101 Transmembrane helix 4 from 94 (in) to 109 (out) : 3.227 Transmembrane helix 5 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 6 from 166 (in) to 181 (out) : 0.0869999 Transmembrane helix 7 from 185 (out) to 200 (in) : 5.553 Transmembrane helix 8 from 204 (in) to 219 (out) : -1.871 Transmembrane helix 9 from 223 (out) to 238 (in) : 0 Transmembrane helix 10 from 242 (in) to 257 (out) : 0 Transmembrane helix 11 from 261 (out) to 276 (in) : 0 Transmembrane helix 12 from 280 (in) to 295 (out) : 0 Transmembrane helix 13 from 299 (out) to 314 (in) : 0 Transmembrane helix 14 from 318 (in) to 333 (out) : 0 Transmembrane helix 15 from 337 (out) to 352 (in) : 0 Transmembrane helix 16 from 356 (in) to 371 (out) : 0 Transmembrane helix 17 from 375 (out) to 390 (in) : 0 Transmembrane helix 18 from 394 (in) to 409 (out) : 0 Transmembrane helix 19 from 413 (out) to 428 (in) : 0 Re-entrant helix 1 from 134 (in) to 162 (in) : 2030 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 69 (out) to 84 (in) : 2.398 Transmembrane helix 3 from 88 (in) to 103 (out) : 4.513 Transmembrane helix 4 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 5 from 166 (in) to 181 (out) : 0.0869999 Transmembrane helix 6 from 185 (out) to 200 (in) : 5.553 Transmembrane helix 7 from 204 (in) to 219 (out) : -1.871 Transmembrane helix 8 from 223 (out) to 238 (in) : 0 Transmembrane helix 9 from 242 (in) to 257 (out) : 0 Transmembrane helix 10 from 261 (out) to 276 (in) : 0 Transmembrane helix 11 from 280 (in) to 295 (out) : 0 Transmembrane helix 12 from 299 (out) to 314 (in) : 0 Transmembrane helix 13 from 318 (in) to 333 (out) : 0 Transmembrane helix 14 from 337 (out) to 352 (in) : 0 Transmembrane helix 15 from 356 (in) to 371 (out) : 0 Transmembrane helix 16 from 375 (out) to 390 (in) : 0 Transmembrane helix 17 from 394 (in) to 409 (out) : 0 Transmembrane helix 18 from 413 (out) to 428 (in) : 0 Re-entrant helix 1 from 134 (in) to 162 (in) : 2030 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 37 (out) to 52 (in) : 28.171 Transmembrane helix 2 from 56 (in) to 71 (out) : 4.459 Transmembrane helix 3 from 75 (out) to 90 (in) : 3.101 Transmembrane helix 4 from 94 (in) to 109 (out) : 3.227 Transmembrane helix 5 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 6 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 7 from 200 (out) to 215 (in) : 1.514 Transmembrane helix 8 from 219 (in) to 234 (out) : 0 Transmembrane helix 9 from 238 (out) to 253 (in) : 0 Transmembrane helix 10 from 257 (in) to 272 (out) : 0 Transmembrane helix 11 from 276 (out) to 291 (in) : 0 Transmembrane helix 12 from 295 (in) to 310 (out) : 0 Transmembrane helix 13 from 314 (out) to 329 (in) : 0 Transmembrane helix 14 from 333 (in) to 348 (out) : 0 Transmembrane helix 15 from 352 (out) to 367 (in) : 0 Transmembrane helix 16 from 371 (in) to 386 (out) : 0 Transmembrane helix 17 from 390 (out) to 405 (in) : 0 Re-entrant helix 1 from 134 (in) to 168 (in) : 2450 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 69 (out) to 84 (in) : 2.398 Transmembrane helix 3 from 88 (in) to 103 (out) : 4.513 Transmembrane helix 4 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 5 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 6 from 200 (out) to 215 (in) : 1.514 Transmembrane helix 7 from 219 (in) to 234 (out) : 0 Transmembrane helix 8 from 238 (out) to 253 (in) : 0 Transmembrane helix 9 from 257 (in) to 272 (out) : 0 Transmembrane helix 10 from 276 (out) to 291 (in) : 0 Transmembrane helix 11 from 295 (in) to 310 (out) : 0 Transmembrane helix 12 from 314 (out) to 329 (in) : 0 Transmembrane helix 13 from 333 (in) to 348 (out) : 0 Transmembrane helix 14 from 352 (out) to 367 (in) : 0 Transmembrane helix 15 from 371 (in) to 386 (out) : 0 Transmembrane helix 16 from 390 (out) to 405 (in) : 0 Re-entrant helix 1 from 134 (in) to 168 (in) : 2450 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 37 (out) to 54 (in) : 29.832 Transmembrane helix 2 from 58 (in) to 73 (out) : 4.996 Transmembrane helix 3 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 4 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 5 from 200 (out) to 215 (in) : 1.514 Transmembrane helix 6 from 219 (in) to 234 (out) : 0 Transmembrane helix 7 from 238 (out) to 253 (in) : 0 Transmembrane helix 8 from 257 (in) to 272 (out) : 0 Transmembrane helix 9 from 276 (out) to 291 (in) : 0 Transmembrane helix 10 from 295 (in) to 310 (out) : 0 Transmembrane helix 11 from 314 (out) to 329 (in) : 0 Transmembrane helix 12 from 333 (in) to 348 (out) : 0 Transmembrane helix 13 from 352 (out) to 367 (in) : 0 Transmembrane helix 14 from 371 (in) to 386 (out) : 0 Transmembrane helix 15 from 390 (out) to 405 (in) : 0 Re-entrant helix 1 from 77 (out) to 111 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 3 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 4 from 200 (out) to 215 (in) : 1.514 Transmembrane helix 5 from 219 (in) to 234 (out) : 0 Transmembrane helix 6 from 238 (out) to 253 (in) : 0 Transmembrane helix 7 from 257 (in) to 272 (out) : 0 Transmembrane helix 8 from 276 (out) to 291 (in) : 0 Transmembrane helix 9 from 295 (in) to 310 (out) : 0 Transmembrane helix 10 from 314 (out) to 329 (in) : 0 Transmembrane helix 11 from 333 (in) to 348 (out) : 0 Transmembrane helix 12 from 352 (out) to 367 (in) : 0 Transmembrane helix 13 from 371 (in) to 386 (out) : 0 Transmembrane helix 14 from 390 (out) to 405 (in) : 0 Re-entrant helix 1 from 69 (out) to 103 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 37 (out) to 54 (in) : 29.832 Transmembrane helix 2 from 58 (in) to 73 (out) : 4.996 Transmembrane helix 3 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 4 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 5 from 253 (out) to 268 (in) : 0 Transmembrane helix 6 from 272 (in) to 287 (out) : 0 Transmembrane helix 7 from 291 (out) to 306 (in) : 0 Transmembrane helix 8 from 310 (in) to 325 (out) : 0 Transmembrane helix 9 from 329 (out) to 344 (in) : 0 Transmembrane helix 10 from 348 (in) to 363 (out) : 0 Transmembrane helix 11 from 367 (out) to 382 (in) : 0 Transmembrane helix 12 from 386 (in) to 401 (out) : 0 Transmembrane helix 13 from 405 (out) to 420 (in) : 0 Re-entrant helix 1 from 77 (out) to 111 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 3 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 4 from 253 (out) to 268 (in) : 0 Transmembrane helix 5 from 272 (in) to 287 (out) : 0 Transmembrane helix 6 from 291 (out) to 306 (in) : 0 Transmembrane helix 7 from 310 (in) to 325 (out) : 0 Transmembrane helix 8 from 329 (out) to 344 (in) : 0 Transmembrane helix 9 from 348 (in) to 363 (out) : 0 Transmembrane helix 10 from 367 (out) to 382 (in) : 0 Transmembrane helix 11 from 386 (in) to 401 (out) : 0 Transmembrane helix 12 from 405 (out) to 420 (in) : 0 Re-entrant helix 1 from 69 (out) to 103 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 37 (out) to 54 (in) : 29.832 Transmembrane helix 2 from 58 (in) to 73 (out) : 4.996 Transmembrane helix 3 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 4 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 5 from 253 (out) to 268 (in) : 0 Transmembrane helix 6 from 272 (in) to 287 (out) : 0 Transmembrane helix 7 from 291 (out) to 306 (in) : 0 Transmembrane helix 8 from 310 (in) to 325 (out) : 0 Transmembrane helix 9 from 329 (out) to 344 (in) : 0 Transmembrane helix 10 from 348 (in) to 363 (out) : 0 Transmembrane helix 11 from 367 (out) to 382 (in) : 0 Re-entrant helix 1 from 77 (out) to 111 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 3 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 4 from 253 (out) to 268 (in) : 0 Transmembrane helix 5 from 272 (in) to 287 (out) : 0 Transmembrane helix 6 from 291 (out) to 306 (in) : 0 Transmembrane helix 7 from 310 (in) to 325 (out) : 0 Transmembrane helix 8 from 329 (out) to 344 (in) : 0 Transmembrane helix 9 from 348 (in) to 363 (out) : 0 Transmembrane helix 10 from 367 (out) to 382 (in) : 0 Re-entrant helix 1 from 69 (out) to 103 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 37 (out) to 54 (in) : 29.832 Transmembrane helix 2 from 58 (in) to 73 (out) : 4.996 Transmembrane helix 3 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 4 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 5 from 253 (out) to 268 (in) : 0 Transmembrane helix 6 from 272 (in) to 287 (out) : 0 Transmembrane helix 7 from 291 (out) to 306 (in) : 0 Transmembrane helix 8 from 310 (in) to 325 (out) : 0 Transmembrane helix 9 from 329 (out) to 344 (in) : 0 Re-entrant helix 1 from 77 (out) to 111 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 3 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 4 from 253 (out) to 268 (in) : 0 Transmembrane helix 5 from 272 (in) to 287 (out) : 0 Transmembrane helix 6 from 291 (out) to 306 (in) : 0 Transmembrane helix 7 from 310 (in) to 325 (out) : 0 Transmembrane helix 8 from 329 (out) to 344 (in) : 0 Re-entrant helix 1 from 69 (out) to 103 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 37 (out) to 54 (in) : 29.832 Transmembrane helix 2 from 58 (in) to 73 (out) : 4.996 Transmembrane helix 3 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 4 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 5 from 253 (out) to 268 (in) : 0 Transmembrane helix 6 from 272 (in) to 287 (out) : 0 Transmembrane helix 7 from 291 (out) to 306 (in) : 0 Re-entrant helix 1 from 77 (out) to 111 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 3 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 4 from 253 (out) to 268 (in) : 0 Transmembrane helix 5 from 272 (in) to 287 (out) : 0 Transmembrane helix 6 from 291 (out) to 306 (in) : 0 Re-entrant helix 1 from 69 (out) to 103 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 37 (out) to 54 (in) : 29.832 Transmembrane helix 2 from 58 (in) to 73 (out) : 4.996 Transmembrane helix 3 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 4 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 5 from 253 (out) to 268 (in) : 0 Re-entrant helix 1 from 77 (out) to 111 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 3 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 4 from 253 (out) to 268 (in) : 0 Re-entrant helix 1 from 69 (out) to 103 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 103 (out) to 130 (in) : 12.96 Transmembrane helix 2 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 3 from 253 (out) to 268 (in) : 0 Re-entrant helix 1 from 134 (in) to 168 (in) : 2450 Re-entrant helix 2 from 195 (out) to 229 (out) : 2450 Re-entrant helix 3 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 123 (out) to 153 (in) : 10.3 Re-entrant helix 1 from 69 (out) to 103 (out) : 2450 Re-entrant helix 2 from 104 (out) to 119 (out) : 1120 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 6 (out) to 21 (in) : -355.339 Transmembrane helix 2 from 33 (in) to 48 (out) : -17.97 Transmembrane helix 3 from 52 (out) to 67 (in) : -3.57 Transmembrane helix 4 from 71 (in) to 86 (out) : -1.819 Transmembrane helix 5 from 90 (out) to 105 (in) : -3.531 Transmembrane helix 6 from 109 (in) to 124 (out) : -6.429 Transmembrane helix 7 from 128 (out) to 143 (in) : 3.286 Transmembrane helix 8 from 147 (in) to 162 (out) : 1.015 Transmembrane helix 9 from 166 (out) to 181 (in) : -0.0869999 Transmembrane helix 10 from 185 (in) to 200 (out) : -5.553 Transmembrane helix 11 from 204 (out) to 219 (in) : 1.871 Transmembrane helix 12 from 223 (in) to 238 (out) : 0 Transmembrane helix 13 from 242 (out) to 257 (in) : 0 Transmembrane helix 14 from 261 (in) to 276 (out) : 0 Transmembrane helix 15 from 280 (out) to 295 (in) : 0 Transmembrane helix 16 from 299 (in) to 314 (out) : 0 Transmembrane helix 17 from 318 (out) to 333 (in) : 0 Transmembrane helix 18 from 337 (in) to 352 (out) : 0 Transmembrane helix 19 from 356 (out) to 371 (in) : 0 Transmembrane helix 20 from 375 (in) to 390 (out) : 0 Transmembrane helix 21 from 394 (out) to 409 (in) : 0 Transmembrane helix 22 from 413 (in) to 428 (out) : 0 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 33 (in) to 48 (out) : -17.97 Transmembrane helix 2 from 52 (out) to 67 (in) : -3.57 Transmembrane helix 3 from 71 (in) to 86 (out) : -1.819 Transmembrane helix 4 from 90 (out) to 105 (in) : -3.531 Transmembrane helix 5 from 109 (in) to 124 (out) : -6.429 Transmembrane helix 6 from 128 (out) to 143 (in) : 3.286 Transmembrane helix 7 from 147 (in) to 162 (out) : 1.015 Transmembrane helix 8 from 166 (out) to 181 (in) : -0.0869999 Transmembrane helix 9 from 185 (in) to 200 (out) : -5.553 Transmembrane helix 10 from 204 (out) to 219 (in) : 1.871 Transmembrane helix 11 from 223 (in) to 238 (out) : 0 Transmembrane helix 12 from 242 (out) to 257 (in) : 0 Transmembrane helix 13 from 261 (in) to 276 (out) : 0 Transmembrane helix 14 from 280 (out) to 295 (in) : 0 Transmembrane helix 15 from 299 (in) to 314 (out) : 0 Transmembrane helix 16 from 318 (out) to 333 (in) : 0 Transmembrane helix 17 from 337 (in) to 352 (out) : 0 Transmembrane helix 18 from 356 (out) to 371 (in) : 0 Transmembrane helix 19 from 375 (in) to 390 (out) : 0 Transmembrane helix 20 from 394 (out) to 409 (in) : 0 Transmembrane helix 21 from 413 (in) to 428 (out) : 0 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 37 (out) to 52 (in) : 28.171 Transmembrane helix 2 from 56 (in) to 71 (out) : 4.459 Transmembrane helix 3 from 75 (out) to 90 (in) : 3.101 Transmembrane helix 4 from 94 (in) to 109 (out) : 3.227 Transmembrane helix 5 from 113 (out) to 128 (in) : 6.52 Transmembrane helix 6 from 132 (in) to 147 (out) : -1.002 Transmembrane helix 7 from 151 (out) to 166 (in) : 0.331 Transmembrane helix 8 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 9 from 200 (out) to 215 (in) : 1.514 Transmembrane helix 10 from 219 (in) to 234 (out) : 0 Transmembrane helix 11 from 238 (out) to 253 (in) : 0 Transmembrane helix 12 from 257 (in) to 272 (out) : 0 Transmembrane helix 13 from 276 (out) to 291 (in) : 0 Transmembrane helix 14 from 295 (in) to 310 (out) : 0 Transmembrane helix 15 from 314 (out) to 329 (in) : 0 Transmembrane helix 16 from 333 (in) to 348 (out) : 0 Transmembrane helix 17 from 352 (out) to 367 (in) : 0 Transmembrane helix 18 from 371 (in) to 386 (out) : 0 Transmembrane helix 19 from 390 (out) to 405 (in) : 0 Transmembrane helix 20 from 409 (in) to 424 (out) : 0 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 37 (in) to 64 (out) : 23.678 Transmembrane helix 2 from 68 (out) to 83 (in) : 1.723 Transmembrane helix 3 from 87 (in) to 102 (out) : 4.405 Transmembrane helix 4 from 106 (out) to 121 (in) : 5.318 Transmembrane helix 5 from 125 (in) to 140 (out) : -3.868 Transmembrane helix 6 from 144 (out) to 159 (in) : 1.646 Transmembrane helix 7 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 8 from 200 (out) to 215 (in) : 1.514 Transmembrane helix 9 from 219 (in) to 234 (out) : 0 Transmembrane helix 10 from 238 (out) to 253 (in) : 0 Transmembrane helix 11 from 257 (in) to 272 (out) : 0 Transmembrane helix 12 from 276 (out) to 291 (in) : 0 Transmembrane helix 13 from 295 (in) to 310 (out) : 0 Transmembrane helix 14 from 314 (out) to 329 (in) : 0 Transmembrane helix 15 from 333 (in) to 348 (out) : 0 Transmembrane helix 16 from 352 (out) to 367 (in) : 0 Transmembrane helix 17 from 371 (in) to 386 (out) : 0 Transmembrane helix 18 from 390 (out) to 405 (in) : 0 Transmembrane helix 19 from 409 (in) to 424 (out) : 0 Re-entrant helix 1 from 163 (in) to 172 (in) : 700 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 37 (out) to 52 (in) : 28.171 Transmembrane helix 2 from 56 (in) to 71 (out) : 4.459 Transmembrane helix 3 from 75 (out) to 90 (in) : 3.101 Transmembrane helix 4 from 94 (in) to 109 (out) : 3.227 Transmembrane helix 5 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 6 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 7 from 200 (out) to 215 (in) : 1.514 Transmembrane helix 8 from 219 (in) to 234 (out) : 0 Transmembrane helix 9 from 238 (out) to 253 (in) : 0 Transmembrane helix 10 from 257 (in) to 272 (out) : 0 Transmembrane helix 11 from 276 (out) to 291 (in) : 0 Transmembrane helix 12 from 295 (in) to 310 (out) : 0 Transmembrane helix 13 from 314 (out) to 329 (in) : 0 Transmembrane helix 14 from 333 (in) to 348 (out) : 0 Transmembrane helix 15 from 352 (out) to 367 (in) : 0 Transmembrane helix 16 from 371 (in) to 386 (out) : 0 Transmembrane helix 17 from 390 (out) to 405 (in) : 0 Transmembrane helix 18 from 409 (in) to 424 (out) : 0 Re-entrant helix 1 from 134 (in) to 168 (in) : 2450 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 69 (out) to 84 (in) : 2.398 Transmembrane helix 3 from 88 (in) to 103 (out) : 4.513 Transmembrane helix 4 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 5 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 6 from 200 (out) to 215 (in) : 1.514 Transmembrane helix 7 from 219 (in) to 234 (out) : 0 Transmembrane helix 8 from 238 (out) to 253 (in) : 0 Transmembrane helix 9 from 257 (in) to 272 (out) : 0 Transmembrane helix 10 from 276 (out) to 291 (in) : 0 Transmembrane helix 11 from 295 (in) to 310 (out) : 0 Transmembrane helix 12 from 314 (out) to 329 (in) : 0 Transmembrane helix 13 from 333 (in) to 348 (out) : 0 Transmembrane helix 14 from 352 (out) to 367 (in) : 0 Transmembrane helix 15 from 371 (in) to 386 (out) : 0 Transmembrane helix 16 from 390 (out) to 405 (in) : 0 Transmembrane helix 17 from 409 (in) to 424 (out) : 0 Re-entrant helix 1 from 134 (in) to 168 (in) : 2450 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 37 (out) to 54 (in) : 29.832 Transmembrane helix 2 from 58 (in) to 73 (out) : 4.996 Transmembrane helix 3 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 4 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 5 from 200 (out) to 215 (in) : 1.514 Transmembrane helix 6 from 219 (in) to 234 (out) : 0 Transmembrane helix 7 from 238 (out) to 253 (in) : 0 Transmembrane helix 8 from 257 (in) to 272 (out) : 0 Transmembrane helix 9 from 276 (out) to 291 (in) : 0 Transmembrane helix 10 from 295 (in) to 310 (out) : 0 Transmembrane helix 11 from 314 (out) to 329 (in) : 0 Transmembrane helix 12 from 333 (in) to 348 (out) : 0 Transmembrane helix 13 from 352 (out) to 367 (in) : 0 Transmembrane helix 14 from 371 (in) to 386 (out) : 0 Transmembrane helix 15 from 390 (out) to 405 (in) : 0 Transmembrane helix 16 from 409 (in) to 424 (out) : 0 Re-entrant helix 1 from 77 (out) to 111 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 3 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 4 from 200 (out) to 215 (in) : 1.514 Transmembrane helix 5 from 219 (in) to 234 (out) : 0 Transmembrane helix 6 from 238 (out) to 253 (in) : 0 Transmembrane helix 7 from 257 (in) to 272 (out) : 0 Transmembrane helix 8 from 276 (out) to 291 (in) : 0 Transmembrane helix 9 from 295 (in) to 310 (out) : 0 Transmembrane helix 10 from 314 (out) to 329 (in) : 0 Transmembrane helix 11 from 333 (in) to 348 (out) : 0 Transmembrane helix 12 from 352 (out) to 367 (in) : 0 Transmembrane helix 13 from 371 (in) to 386 (out) : 0 Transmembrane helix 14 from 390 (out) to 405 (in) : 0 Transmembrane helix 15 from 409 (in) to 424 (out) : 0 Re-entrant helix 1 from 69 (out) to 103 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 37 (out) to 54 (in) : 29.832 Transmembrane helix 2 from 58 (in) to 73 (out) : 4.996 Transmembrane helix 3 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 4 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 5 from 200 (out) to 215 (in) : 1.514 Transmembrane helix 6 from 219 (in) to 234 (out) : 0 Transmembrane helix 7 from 238 (out) to 253 (in) : 0 Transmembrane helix 8 from 257 (in) to 272 (out) : 0 Transmembrane helix 9 from 276 (out) to 291 (in) : 0 Transmembrane helix 10 from 295 (in) to 310 (out) : 0 Transmembrane helix 11 from 314 (out) to 329 (in) : 0 Transmembrane helix 12 from 333 (in) to 348 (out) : 0 Transmembrane helix 13 from 352 (out) to 367 (in) : 0 Transmembrane helix 14 from 371 (in) to 386 (out) : 0 Re-entrant helix 1 from 77 (out) to 111 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 3 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 4 from 200 (out) to 215 (in) : 1.514 Transmembrane helix 5 from 219 (in) to 234 (out) : 0 Transmembrane helix 6 from 238 (out) to 253 (in) : 0 Transmembrane helix 7 from 257 (in) to 272 (out) : 0 Transmembrane helix 8 from 276 (out) to 291 (in) : 0 Transmembrane helix 9 from 295 (in) to 310 (out) : 0 Transmembrane helix 10 from 314 (out) to 329 (in) : 0 Transmembrane helix 11 from 333 (in) to 348 (out) : 0 Transmembrane helix 12 from 352 (out) to 367 (in) : 0 Transmembrane helix 13 from 371 (in) to 386 (out) : 0 Re-entrant helix 1 from 69 (out) to 103 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 37 (out) to 54 (in) : 29.832 Transmembrane helix 2 from 58 (in) to 73 (out) : 4.996 Transmembrane helix 3 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 4 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 5 from 253 (out) to 268 (in) : 0 Transmembrane helix 6 from 272 (in) to 287 (out) : 0 Transmembrane helix 7 from 291 (out) to 306 (in) : 0 Transmembrane helix 8 from 310 (in) to 325 (out) : 0 Transmembrane helix 9 from 329 (out) to 344 (in) : 0 Transmembrane helix 10 from 348 (in) to 363 (out) : 0 Transmembrane helix 11 from 367 (out) to 382 (in) : 0 Transmembrane helix 12 from 386 (in) to 401 (out) : 0 Re-entrant helix 1 from 77 (out) to 111 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 3 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 4 from 253 (out) to 268 (in) : 0 Transmembrane helix 5 from 272 (in) to 287 (out) : 0 Transmembrane helix 6 from 291 (out) to 306 (in) : 0 Transmembrane helix 7 from 310 (in) to 325 (out) : 0 Transmembrane helix 8 from 329 (out) to 344 (in) : 0 Transmembrane helix 9 from 348 (in) to 363 (out) : 0 Transmembrane helix 10 from 367 (out) to 382 (in) : 0 Transmembrane helix 11 from 386 (in) to 401 (out) : 0 Re-entrant helix 1 from 69 (out) to 103 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 37 (out) to 54 (in) : 29.832 Transmembrane helix 2 from 58 (in) to 73 (out) : 4.996 Transmembrane helix 3 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 4 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 5 from 253 (out) to 268 (in) : 0 Transmembrane helix 6 from 272 (in) to 287 (out) : 0 Transmembrane helix 7 from 291 (out) to 306 (in) : 0 Transmembrane helix 8 from 310 (in) to 325 (out) : 0 Transmembrane helix 9 from 329 (out) to 344 (in) : 0 Transmembrane helix 10 from 348 (in) to 363 (out) : 0 Re-entrant helix 1 from 77 (out) to 111 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 3 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 4 from 253 (out) to 268 (in) : 0 Transmembrane helix 5 from 272 (in) to 287 (out) : 0 Transmembrane helix 6 from 291 (out) to 306 (in) : 0 Transmembrane helix 7 from 310 (in) to 325 (out) : 0 Transmembrane helix 8 from 329 (out) to 344 (in) : 0 Transmembrane helix 9 from 348 (in) to 363 (out) : 0 Re-entrant helix 1 from 69 (out) to 103 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 37 (out) to 54 (in) : 29.832 Transmembrane helix 2 from 58 (in) to 73 (out) : 4.996 Transmembrane helix 3 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 4 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 5 from 253 (out) to 268 (in) : 0 Transmembrane helix 6 from 272 (in) to 287 (out) : 0 Transmembrane helix 7 from 291 (out) to 306 (in) : 0 Transmembrane helix 8 from 310 (in) to 325 (out) : 0 Re-entrant helix 1 from 77 (out) to 111 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 3 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 4 from 253 (out) to 268 (in) : 0 Transmembrane helix 5 from 272 (in) to 287 (out) : 0 Transmembrane helix 6 from 291 (out) to 306 (in) : 0 Transmembrane helix 7 from 310 (in) to 325 (out) : 0 Re-entrant helix 1 from 69 (out) to 103 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 37 (out) to 54 (in) : 29.832 Transmembrane helix 2 from 58 (in) to 73 (out) : 4.996 Transmembrane helix 3 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 4 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 5 from 253 (out) to 268 (in) : 0 Transmembrane helix 6 from 272 (in) to 287 (out) : 0 Re-entrant helix 1 from 77 (out) to 111 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 3 from 176 (in) to 191 (out) : 15.682 Transmembrane helix 4 from 253 (out) to 268 (in) : 0 Transmembrane helix 5 from 272 (in) to 287 (out) : 0 Re-entrant helix 1 from 69 (out) to 103 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Re-entrant helix 3 from 195 (out) to 229 (out) : 2450 Re-entrant helix 4 from 230 (out) to 249 (out) : 1400 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 37 (out) to 54 (in) : 29.832 Transmembrane helix 2 from 58 (in) to 73 (out) : 4.996 Transmembrane helix 3 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 4 from 176 (in) to 191 (out) : 15.682 Re-entrant helix 1 from 77 (out) to 111 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 37 (in) to 65 (out) : 23.908 Transmembrane helix 2 from 115 (out) to 130 (in) : 6.747 Transmembrane helix 3 from 176 (in) to 191 (out) : 15.682 Re-entrant helix 1 from 69 (out) to 103 (out) : 2450 Re-entrant helix 2 from 134 (in) to 168 (in) : 2450 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 103 (out) to 130 (in) : 12.96 Transmembrane helix 2 from 176 (in) to 191 (out) : 15.682 Re-entrant helix 1 from 134 (in) to 168 (in) : 2450 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.019825 1 helix (-) : Score = 0.040438 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 22 helices (+) : Score = -100000 22 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 37-57 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.040438