Processing 1 helix: Transmembrane helix 1 from 119 (in) to 149 (out) : 14.098 Score = 0.014098 Processing 1 helix: Transmembrane helix 1 from 153 (out) to 182 (in) : 12.717 Score = 0.012717 Processing 16 helices: Transmembrane helix 1 from 11 (in) to 26 (out) : -2004.42 Transmembrane helix 2 from 30 (out) to 45 (in) : -846.744 Transmembrane helix 3 from 49 (in) to 64 (out) : -1335.46 Transmembrane helix 4 from 68 (out) to 83 (in) : -535.788 Transmembrane helix 5 from 87 (in) to 102 (out) : 0.558 Transmembrane helix 6 from 106 (out) to 121 (in) : 2.208 Transmembrane helix 7 from 125 (in) to 140 (out) : 0.0830001 Transmembrane helix 8 from 144 (out) to 159 (in) : -0.163 Transmembrane helix 9 from 163 (in) to 178 (out) : -9.533 Transmembrane helix 10 from 182 (out) to 197 (in) : -4.954 Transmembrane helix 11 from 201 (in) to 216 (out) : 0 Transmembrane helix 12 from 220 (out) to 235 (in) : 0 Transmembrane helix 13 from 239 (in) to 254 (out) : 0 Transmembrane helix 14 from 258 (out) to 273 (in) : 0 Transmembrane helix 15 from 277 (in) to 292 (out) : 0 Transmembrane helix 16 from 296 (out) to 311 (in) : 0 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : -885.013 Transmembrane helix 2 from 34 (in) to 49 (out) : -908.176 Transmembrane helix 3 from 68 (out) to 83 (in) : -535.788 Transmembrane helix 4 from 87 (in) to 102 (out) : 0.558 Transmembrane helix 5 from 106 (out) to 121 (in) : 2.208 Transmembrane helix 6 from 125 (in) to 140 (out) : 0.0830001 Transmembrane helix 7 from 144 (out) to 159 (in) : -0.163 Transmembrane helix 8 from 163 (in) to 178 (out) : -9.533 Transmembrane helix 9 from 182 (out) to 197 (in) : -4.954 Transmembrane helix 10 from 201 (in) to 216 (out) : 0 Transmembrane helix 11 from 220 (out) to 235 (in) : 0 Transmembrane helix 12 from 239 (in) to 254 (out) : 0 Transmembrane helix 13 from 258 (out) to 273 (in) : 0 Transmembrane helix 14 from 277 (in) to 292 (out) : 0 Transmembrane helix 15 from 296 (out) to 311 (in) : 0 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 29 (in) to 44 (out) : -841.465 Transmembrane helix 2 from 68 (out) to 83 (in) : -535.788 Transmembrane helix 3 from 87 (in) to 102 (out) : 0.558 Transmembrane helix 4 from 106 (out) to 121 (in) : 2.208 Transmembrane helix 5 from 125 (in) to 140 (out) : 0.0830001 Transmembrane helix 6 from 144 (out) to 159 (in) : -0.163 Transmembrane helix 7 from 163 (in) to 178 (out) : -9.533 Transmembrane helix 8 from 182 (out) to 197 (in) : -4.954 Transmembrane helix 9 from 201 (in) to 216 (out) : 0 Transmembrane helix 10 from 220 (out) to 235 (in) : 0 Transmembrane helix 11 from 239 (in) to 254 (out) : 0 Transmembrane helix 12 from 258 (out) to 273 (in) : 0 Transmembrane helix 13 from 277 (in) to 292 (out) : 0 Transmembrane helix 14 from 296 (out) to 311 (in) : 0 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 68 (out) to 83 (in) : -535.788 Transmembrane helix 2 from 87 (in) to 102 (out) : 0.558 Transmembrane helix 3 from 106 (out) to 121 (in) : 2.208 Transmembrane helix 4 from 125 (in) to 140 (out) : 0.0830001 Transmembrane helix 5 from 144 (out) to 159 (in) : -0.163 Transmembrane helix 6 from 163 (in) to 178 (out) : -9.533 Transmembrane helix 7 from 182 (out) to 197 (in) : -4.954 Transmembrane helix 8 from 201 (in) to 216 (out) : 0 Transmembrane helix 9 from 220 (out) to 235 (in) : 0 Transmembrane helix 10 from 239 (in) to 254 (out) : 0 Transmembrane helix 11 from 258 (out) to 273 (in) : 0 Transmembrane helix 12 from 277 (in) to 292 (out) : 0 Transmembrane helix 13 from 296 (out) to 311 (in) : 0 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 80 (in) to 95 (out) : -6.099 Transmembrane helix 2 from 99 (out) to 114 (in) : 5.304 Transmembrane helix 3 from 125 (in) to 140 (out) : 0.0830001 Transmembrane helix 4 from 144 (out) to 159 (in) : -0.163 Transmembrane helix 5 from 163 (in) to 178 (out) : -9.533 Transmembrane helix 6 from 182 (out) to 197 (in) : -4.954 Transmembrane helix 7 from 201 (in) to 216 (out) : 0 Transmembrane helix 8 from 220 (out) to 235 (in) : 0 Transmembrane helix 9 from 239 (in) to 254 (out) : 0 Transmembrane helix 10 from 258 (out) to 273 (in) : 0 Transmembrane helix 11 from 277 (in) to 292 (out) : 0 Transmembrane helix 12 from 296 (out) to 311 (in) : 0 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 80 (out) to 95 (in) : 6.099 Transmembrane helix 2 from 107 (in) to 122 (out) : -1.716 Transmembrane helix 3 from 126 (out) to 141 (in) : 0.344 Transmembrane helix 4 from 145 (in) to 160 (out) : 0.279 Transmembrane helix 5 from 164 (out) to 179 (in) : 9.344 Transmembrane helix 6 from 183 (in) to 198 (out) : 3.242 Transmembrane helix 7 from 202 (out) to 217 (in) : 0 Transmembrane helix 8 from 221 (in) to 236 (out) : 0 Transmembrane helix 9 from 240 (out) to 255 (in) : 0 Transmembrane helix 10 from 259 (in) to 274 (out) : 0 Transmembrane helix 11 from 278 (out) to 293 (in) : 0 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 80 (in) to 95 (out) : -6.099 Transmembrane helix 2 from 99 (out) to 114 (in) : 5.304 Transmembrane helix 3 from 137 (in) to 152 (out) : 8.018 Transmembrane helix 4 from 162 (out) to 177 (in) : 8.92 Transmembrane helix 5 from 181 (in) to 196 (out) : 6.547 Transmembrane helix 6 from 200 (out) to 215 (in) : 0 Transmembrane helix 7 from 219 (in) to 234 (out) : 0 Transmembrane helix 8 from 238 (out) to 253 (in) : 0 Transmembrane helix 9 from 257 (in) to 272 (out) : 0 Transmembrane helix 10 from 276 (out) to 291 (in) : 0 Re-entrant helix 1 from 118 (in) to 133 (in) : 1120 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 80 (out) to 95 (in) : 6.099 Transmembrane helix 2 from 137 (in) to 152 (out) : 8.018 Transmembrane helix 3 from 162 (out) to 177 (in) : 8.92 Transmembrane helix 4 from 181 (in) to 196 (out) : 6.547 Transmembrane helix 5 from 200 (out) to 215 (in) : 0 Transmembrane helix 6 from 219 (in) to 234 (out) : 0 Transmembrane helix 7 from 238 (out) to 253 (in) : 0 Transmembrane helix 8 from 257 (in) to 272 (out) : 0 Transmembrane helix 9 from 276 (out) to 291 (in) : 0 Re-entrant helix 1 from 99 (in) to 133 (in) : 2450 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 80 (in) to 95 (out) : -6.099 Transmembrane helix 2 from 99 (out) to 114 (in) : 5.304 Transmembrane helix 3 from 137 (in) to 152 (out) : 8.018 Transmembrane helix 4 from 162 (out) to 177 (in) : 8.92 Transmembrane helix 5 from 181 (in) to 196 (out) : 6.547 Transmembrane helix 6 from 200 (out) to 215 (in) : 0 Transmembrane helix 7 from 219 (in) to 234 (out) : 0 Transmembrane helix 8 from 238 (out) to 253 (in) : 0 Re-entrant helix 1 from 118 (in) to 133 (in) : 1120 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 80 (out) to 95 (in) : 6.099 Transmembrane helix 2 from 137 (in) to 152 (out) : 8.018 Transmembrane helix 3 from 162 (out) to 177 (in) : 8.92 Transmembrane helix 4 from 181 (in) to 196 (out) : 6.547 Transmembrane helix 5 from 200 (out) to 215 (in) : 0 Transmembrane helix 6 from 219 (in) to 234 (out) : 0 Transmembrane helix 7 from 238 (out) to 253 (in) : 0 Re-entrant helix 1 from 99 (in) to 133 (in) : 2450 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 80 (in) to 95 (out) : -6.099 Transmembrane helix 2 from 99 (out) to 114 (in) : 5.304 Transmembrane helix 3 from 137 (in) to 152 (out) : 8.018 Transmembrane helix 4 from 162 (out) to 177 (in) : 8.92 Transmembrane helix 5 from 181 (in) to 196 (out) : 6.547 Transmembrane helix 6 from 200 (out) to 215 (in) : 0 Re-entrant helix 1 from 118 (in) to 133 (in) : 1120 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 80 (out) to 95 (in) : 6.099 Transmembrane helix 2 from 137 (in) to 152 (out) : 8.018 Transmembrane helix 3 from 162 (out) to 177 (in) : 8.92 Transmembrane helix 4 from 181 (in) to 196 (out) : 6.547 Transmembrane helix 5 from 200 (out) to 215 (in) : 0 Re-entrant helix 1 from 99 (in) to 133 (in) : 2450 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 80 (in) to 95 (out) : -6.099 Transmembrane helix 2 from 99 (out) to 114 (in) : 5.304 Transmembrane helix 3 from 137 (in) to 152 (out) : 8.018 Transmembrane helix 4 from 162 (out) to 182 (in) : 12.156 Re-entrant helix 1 from 118 (in) to 133 (in) : 1120 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 80 (out) to 95 (in) : 6.099 Transmembrane helix 2 from 137 (in) to 152 (out) : 8.018 Transmembrane helix 3 from 162 (out) to 182 (in) : 12.156 Re-entrant helix 1 from 99 (in) to 133 (in) : 2450 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 119 (in) to 149 (out) : 14.098 Transmembrane helix 2 from 162 (out) to 182 (in) : 12.156 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 11 (out) to 26 (in) : -2002.68 Transmembrane helix 2 from 30 (in) to 45 (out) : -852.356 Transmembrane helix 3 from 49 (out) to 64 (in) : -1335.44 Transmembrane helix 4 from 68 (in) to 83 (out) : -519.812 Transmembrane helix 5 from 87 (out) to 102 (in) : -0.558 Transmembrane helix 6 from 106 (in) to 121 (out) : -2.208 Transmembrane helix 7 from 125 (out) to 140 (in) : -0.0830001 Transmembrane helix 8 from 144 (in) to 159 (out) : 0.163 Transmembrane helix 9 from 163 (out) to 178 (in) : 9.533 Transmembrane helix 10 from 182 (in) to 197 (out) : 4.954 Transmembrane helix 11 from 201 (out) to 216 (in) : 0 Transmembrane helix 12 from 220 (in) to 235 (out) : 0 Transmembrane helix 13 from 239 (out) to 254 (in) : 0 Transmembrane helix 14 from 258 (in) to 273 (out) : 0 Transmembrane helix 15 from 277 (out) to 292 (in) : 0 Transmembrane helix 16 from 296 (in) to 311 (out) : 0 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : -884.887 Transmembrane helix 2 from 34 (out) to 49 (in) : -897.924 Transmembrane helix 3 from 68 (in) to 83 (out) : -519.812 Transmembrane helix 4 from 87 (out) to 102 (in) : -0.558 Transmembrane helix 5 from 106 (in) to 121 (out) : -2.208 Transmembrane helix 6 from 125 (out) to 140 (in) : -0.0830001 Transmembrane helix 7 from 144 (in) to 159 (out) : 0.163 Transmembrane helix 8 from 163 (out) to 178 (in) : 9.533 Transmembrane helix 9 from 182 (in) to 197 (out) : 4.954 Transmembrane helix 10 from 201 (out) to 216 (in) : 0 Transmembrane helix 11 from 220 (in) to 235 (out) : 0 Transmembrane helix 12 from 239 (out) to 254 (in) : 0 Transmembrane helix 13 from 258 (in) to 273 (out) : 0 Transmembrane helix 14 from 277 (out) to 292 (in) : 0 Transmembrane helix 15 from 296 (in) to 311 (out) : 0 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 28 (out) to 43 (in) : -839.547 Transmembrane helix 2 from 68 (in) to 83 (out) : -519.812 Transmembrane helix 3 from 87 (out) to 102 (in) : -0.558 Transmembrane helix 4 from 106 (in) to 121 (out) : -2.208 Transmembrane helix 5 from 125 (out) to 140 (in) : -0.0830001 Transmembrane helix 6 from 144 (in) to 159 (out) : 0.163 Transmembrane helix 7 from 163 (out) to 178 (in) : 9.533 Transmembrane helix 8 from 182 (in) to 197 (out) : 4.954 Transmembrane helix 9 from 201 (out) to 216 (in) : 0 Transmembrane helix 10 from 220 (in) to 235 (out) : 0 Transmembrane helix 11 from 239 (out) to 254 (in) : 0 Transmembrane helix 12 from 258 (in) to 273 (out) : 0 Transmembrane helix 13 from 277 (out) to 292 (in) : 0 Transmembrane helix 14 from 296 (in) to 311 (out) : 0 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 68 (in) to 83 (out) : -519.812 Transmembrane helix 2 from 87 (out) to 102 (in) : -0.558 Transmembrane helix 3 from 106 (in) to 121 (out) : -2.208 Transmembrane helix 4 from 125 (out) to 140 (in) : -0.0830001 Transmembrane helix 5 from 144 (in) to 159 (out) : 0.163 Transmembrane helix 6 from 163 (out) to 178 (in) : 9.533 Transmembrane helix 7 from 182 (in) to 197 (out) : 4.954 Transmembrane helix 8 from 201 (out) to 216 (in) : 0 Transmembrane helix 9 from 220 (in) to 235 (out) : 0 Transmembrane helix 10 from 239 (out) to 254 (in) : 0 Transmembrane helix 11 from 258 (in) to 273 (out) : 0 Transmembrane helix 12 from 277 (out) to 292 (in) : 0 Transmembrane helix 13 from 296 (in) to 311 (out) : 0 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 80 (out) to 95 (in) : 6.099 Transmembrane helix 2 from 106 (in) to 121 (out) : -2.208 Transmembrane helix 3 from 125 (out) to 140 (in) : -0.0830001 Transmembrane helix 4 from 144 (in) to 159 (out) : 0.163 Transmembrane helix 5 from 163 (out) to 178 (in) : 9.533 Transmembrane helix 6 from 182 (in) to 197 (out) : 4.954 Transmembrane helix 7 from 201 (out) to 216 (in) : 0 Transmembrane helix 8 from 220 (in) to 235 (out) : 0 Transmembrane helix 9 from 239 (out) to 254 (in) : 0 Transmembrane helix 10 from 258 (in) to 273 (out) : 0 Transmembrane helix 11 from 277 (out) to 292 (in) : 0 Transmembrane helix 12 from 296 (in) to 311 (out) : 0 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 80 (in) to 95 (out) : -6.099 Transmembrane helix 2 from 99 (out) to 114 (in) : 5.304 Transmembrane helix 3 from 137 (in) to 152 (out) : 8.018 Transmembrane helix 4 from 162 (out) to 177 (in) : 8.92 Transmembrane helix 5 from 181 (in) to 196 (out) : 6.547 Transmembrane helix 6 from 200 (out) to 215 (in) : 0 Transmembrane helix 7 from 219 (in) to 234 (out) : 0 Transmembrane helix 8 from 238 (out) to 253 (in) : 0 Transmembrane helix 9 from 257 (in) to 272 (out) : 0 Transmembrane helix 10 from 276 (out) to 291 (in) : 0 Transmembrane helix 11 from 295 (in) to 310 (out) : 0 Re-entrant helix 1 from 118 (in) to 133 (in) : 1120 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 80 (out) to 95 (in) : 6.099 Transmembrane helix 2 from 137 (in) to 152 (out) : 8.018 Transmembrane helix 3 from 162 (out) to 177 (in) : 8.92 Transmembrane helix 4 from 181 (in) to 196 (out) : 6.547 Transmembrane helix 5 from 200 (out) to 215 (in) : 0 Transmembrane helix 6 from 219 (in) to 234 (out) : 0 Transmembrane helix 7 from 238 (out) to 253 (in) : 0 Transmembrane helix 8 from 257 (in) to 272 (out) : 0 Transmembrane helix 9 from 276 (out) to 291 (in) : 0 Transmembrane helix 10 from 295 (in) to 310 (out) : 0 Re-entrant helix 1 from 99 (in) to 133 (in) : 2450 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 80 (in) to 95 (out) : -6.099 Transmembrane helix 2 from 99 (out) to 114 (in) : 5.304 Transmembrane helix 3 from 137 (in) to 152 (out) : 8.018 Transmembrane helix 4 from 162 (out) to 177 (in) : 8.92 Transmembrane helix 5 from 181 (in) to 196 (out) : 6.547 Transmembrane helix 6 from 200 (out) to 215 (in) : 0 Transmembrane helix 7 from 219 (in) to 234 (out) : 0 Transmembrane helix 8 from 238 (out) to 253 (in) : 0 Transmembrane helix 9 from 257 (in) to 272 (out) : 0 Re-entrant helix 1 from 118 (in) to 133 (in) : 1120 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 80 (out) to 95 (in) : 6.099 Transmembrane helix 2 from 137 (in) to 152 (out) : 8.018 Transmembrane helix 3 from 162 (out) to 177 (in) : 8.92 Transmembrane helix 4 from 181 (in) to 196 (out) : 6.547 Transmembrane helix 5 from 200 (out) to 215 (in) : 0 Transmembrane helix 6 from 219 (in) to 234 (out) : 0 Transmembrane helix 7 from 238 (out) to 253 (in) : 0 Transmembrane helix 8 from 257 (in) to 272 (out) : 0 Re-entrant helix 1 from 99 (in) to 133 (in) : 2450 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 80 (in) to 95 (out) : -6.099 Transmembrane helix 2 from 99 (out) to 114 (in) : 5.304 Transmembrane helix 3 from 137 (in) to 152 (out) : 8.018 Transmembrane helix 4 from 162 (out) to 177 (in) : 8.92 Transmembrane helix 5 from 181 (in) to 196 (out) : 6.547 Transmembrane helix 6 from 200 (out) to 215 (in) : 0 Transmembrane helix 7 from 219 (in) to 234 (out) : 0 Re-entrant helix 1 from 118 (in) to 133 (in) : 1120 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 80 (out) to 95 (in) : 6.099 Transmembrane helix 2 from 137 (in) to 152 (out) : 8.018 Transmembrane helix 3 from 162 (out) to 177 (in) : 8.92 Transmembrane helix 4 from 181 (in) to 196 (out) : 6.547 Transmembrane helix 5 from 200 (out) to 215 (in) : 0 Transmembrane helix 6 from 219 (in) to 234 (out) : 0 Re-entrant helix 1 from 99 (in) to 133 (in) : 2450 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 80 (in) to 95 (out) : -6.099 Transmembrane helix 2 from 99 (out) to 114 (in) : 5.304 Transmembrane helix 3 from 137 (in) to 152 (out) : 8.018 Transmembrane helix 4 from 162 (out) to 177 (in) : 8.92 Transmembrane helix 5 from 181 (in) to 196 (out) : 6.547 Re-entrant helix 1 from 118 (in) to 133 (in) : 1120 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 80 (out) to 95 (in) : 6.099 Transmembrane helix 2 from 137 (in) to 152 (out) : 8.018 Transmembrane helix 3 from 162 (out) to 177 (in) : 8.92 Transmembrane helix 4 from 181 (in) to 196 (out) : 6.547 Re-entrant helix 1 from 99 (in) to 133 (in) : 2450 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 80 (in) to 95 (out) : -6.099 Transmembrane helix 2 from 99 (out) to 114 (in) : 5.304 Transmembrane helix 3 from 175 (in) to 196 (out) : 11.506 Re-entrant helix 1 from 118 (in) to 152 (in) : 2450 Re-entrant helix 2 from 153 (in) to 171 (in) : 1330 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 80 (out) to 95 (in) : 6.099 Transmembrane helix 2 from 137 (in) to 156 (out) : 8.744 Re-entrant helix 1 from 99 (in) to 133 (in) : 2450 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.014098 1 helix (-) : Score = 0.012717 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 17.409 Topology: 119-149 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.014098