BLASTP 2.2.22 [Sep-27-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= batch____ (89 letters) Database: uniref50.fasta 3,077,464 sequences; 1,040,396,356 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value UniRef50_P75786 Putative uncharacterized protein yliL n=7 Tax=Es... 185 3e-46 UniRef50_A9MSS7 Putative uncharacterized protein n=3 Tax=Salmone... 44 0.002 >UniRef50_P75786 Putative uncharacterized protein yliL n=7 Tax=Escherichia coli RepID=YLIL_ECOLI Length = 89 Score = 185 bits (470), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 89/89 (100%), Positives = 89/89 (100%) Query: 1 MERGPHPRAAIYAGLQEDRASRRSGQLLTNKSFAKGIANPTKSGVFQTKTRYACMKAGVK 60 MERGPHPRAAIYAGLQEDRASRRSGQLLTNKSFAKGIANPTKSGVFQTKTRYACMKAGVK Sbjct: 1 MERGPHPRAAIYAGLQEDRASRRSGQLLTNKSFAKGIANPTKSGVFQTKTRYACMKAGVK 60 Query: 61 RRTATPLFIGRFILLRFVVDHIAQHREQH 89 RRTATPLFIGRFILLRFVVDHIAQHREQH Sbjct: 61 RRTATPLFIGRFILLRFVVDHIAQHREQH 89 >UniRef50_A9MSS7 Putative uncharacterized protein n=3 Tax=Salmonella enterica subsp. enterica RepID=A9MSS7_SALPB Length = 56 Score = 43.9 bits (102), Expect = 0.002, Method: Compositional matrix adjust. Identities = 23/35 (65%), Positives = 24/35 (68%), Gaps = 1/35 (2%) Query: 55 MKAGVKRRTATPLFIGRFILLRFVVDHIAQHREQH 89 M+ RTA LF GR IL RF VDHIAQHREQH Sbjct: 23 MQNKAAYRTAA-LFRGRVILFRFAVDHIAQHREQH 56 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: UniRef50_P75786 Putative uncharacterized protein yliL n=7 Tax=Es... 162 4e-39 UniRef50_A9MSS7 Putative uncharacterized protein n=3 Tax=Salmone... 57 2e-07 Sequences not found previously or not previously below threshold: CONVERGED! >UniRef50_P75786 Putative uncharacterized protein yliL n=7 Tax=Escherichia coli RepID=YLIL_ECOLI Length = 89 Score = 162 bits (409), Expect = 4e-39, Method: Composition-based stats. Identities = 89/89 (100%), Positives = 89/89 (100%) Query: 1 MERGPHPRAAIYAGLQEDRASRRSGQLLTNKSFAKGIANPTKSGVFQTKTRYACMKAGVK 60 MERGPHPRAAIYAGLQEDRASRRSGQLLTNKSFAKGIANPTKSGVFQTKTRYACMKAGVK Sbjct: 1 MERGPHPRAAIYAGLQEDRASRRSGQLLTNKSFAKGIANPTKSGVFQTKTRYACMKAGVK 60 Query: 61 RRTATPLFIGRFILLRFVVDHIAQHREQH 89 RRTATPLFIGRFILLRFVVDHIAQHREQH Sbjct: 61 RRTATPLFIGRFILLRFVVDHIAQHREQH 89 >UniRef50_A9MSS7 Putative uncharacterized protein n=3 Tax=Salmonella enterica subsp. enterica RepID=A9MSS7_SALPB Length = 56 Score = 57.4 bits (137), Expect = 2e-07, Method: Composition-based stats. Identities = 23/35 (65%), Positives = 24/35 (68%), Gaps = 1/35 (2%) Query: 55 MKAGVKRRTATPLFIGRFILLRFVVDHIAQHREQH 89 M+ RTA LF GR IL RF VDHIAQHREQH Sbjct: 23 MQNKAAYRTAA-LFRGRVILFRFAVDHIAQHREQH 56 Database: uniref50.fasta Posted date: Mar 8, 2010 10:38 AM Number of letters in database: 1,040,396,356 Number of sequences in database: 3,077,464 Lambda K H 0.318 0.133 0.383 Lambda K H 0.267 0.0408 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 342,286,280 Number of Sequences: 3077464 Number of extensions: 10809186 Number of successful extensions: 25894 Number of sequences better than 1.0e-01: 2 Number of HSP's better than 0.1 without gapping: 4 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 25889 Number of HSP's gapped (non-prelim): 4 length of query: 89 length of database: 1,040,396,356 effective HSP length: 59 effective length of query: 30 effective length of database: 858,825,980 effective search space: 25764779400 effective search space used: 25764779400 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.3 bits) S2: 87 (38.1 bits)