BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (41 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P0ADB6 Entericidin A n=91 Tax=Enterobacteriaceae RepID=...    83   3e-15
UniRef50_P0ADB9 Entericidin B n=61 Tax=Enterobacteriaceae RepID=...    45   0.001
UniRef50_B0VRY4 Bacteriolytic lipoprotein entericidin B n=11 Tax...    45   0.001
UniRef50_B9XKK9 Entericidin EcnAB n=1 Tax=bacterium Ellin514 Rep...    44   0.001
UniRef50_C9Y199 Putative uncharacterized protein n=2 Tax=Cronoba...    44   0.001
UniRef50_A8G8T1 Entericidin EcnAB n=2 Tax=Serratia RepID=A8G8T1_...    44   0.002
UniRef50_C9Y198 Entericidin B n=15 Tax=Enterobacteriaceae RepID=...    43   0.002
UniRef50_UPI0001826DD8 entericidin EcnAB n=1 Tax=Enterobacter ca...    42   0.005
UniRef50_B5WFA4 Entericidin EcnAB n=2 Tax=Burkholderia RepID=B5W...    41   0.013
UniRef50_D0S831 Predicted protein n=3 Tax=Acinetobacter RepID=D0...    39   0.035
UniRef50_D1RI74 Conserved domain protein n=1 Tax=Legionella long...    39   0.039
UniRef50_Q6FE13 Bacteriolytic lipoprotein entericidin B n=3 Tax=...    39   0.043
UniRef50_C1DD60 Putative uncharacterized protein n=1 Tax=Laribac...    39   0.052
UniRef50_B2T9N9 Entericidin EcnAB n=6 Tax=Burkholderia RepID=B2T...    39   0.057

>UniRef50_P0ADB6 Entericidin A n=91 Tax=Enterobacteriaceae RepID=ECNA_ECO57
          Length = 41

 Score = 82.8 bits (203), Expect = 3e-15,   Method: Compositional matrix adjust.
 Identities = 41/41 (100%), Positives = 41/41 (100%)

Query: 1  MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS 41
          MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS
Sbjct: 1  MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS 41


>UniRef50_P0ADB9 Entericidin B n=61 Tax=Enterobacteriaceae RepID=ECNB_ECO57
          Length = 48

 Score = 44.7 bits (104), Expect = 0.001,   Method: Compositional matrix adjust.
 Identities = 24/44 (54%), Positives = 30/44 (68%), Gaps = 3/44 (6%)

Query: 1  MMKRLIVL---VLLASTLLTGCNTARGFGEDIKHLGNSISRAAS 41
          M+K+ I     VL+ ST+LT CNT RG GEDI   GN+IS AA+
Sbjct: 1  MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT 44


>UniRef50_B0VRY4 Bacteriolytic lipoprotein entericidin B n=11 Tax=Moraxellaceae
          RepID=B0VRY4_ACIBS
          Length = 47

 Score = 44.7 bits (104), Expect = 0.001,   Method: Compositional matrix adjust.
 Identities = 16/41 (39%), Positives = 30/41 (73%)

Query: 1  MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS 41
          MMK+++V  ++ + +LTGCNT +GFG+D+   G++++  A 
Sbjct: 1  MMKKVLVASVMVAFVLTGCNTLKGFGQDVSKAGDAVTNTAQ 41


>UniRef50_B9XKK9 Entericidin EcnAB n=1 Tax=bacterium Ellin514 RepID=B9XKK9_9BACT
          Length = 44

 Score = 44.3 bits (103), Expect = 0.001,   Method: Compositional matrix adjust.
 Identities = 21/41 (51%), Positives = 31/41 (75%), Gaps = 4/41 (9%)

Query: 2  MKRLIVLVLLASTLL----TGCNTARGFGEDIKHLGNSISR 38
          MK  +VL+L+A++LL    TGCNTA GFG+D+ + G++I R
Sbjct: 1  MKTRLVLILIATSLLVMAGTGCNTAHGFGKDVSNTGDAIQR 41


>UniRef50_C9Y199 Putative uncharacterized protein n=2 Tax=Cronobacter
          RepID=C9Y199_CROTZ
          Length = 43

 Score = 43.9 bits (102), Expect = 0.001,   Method: Compositional matrix adjust.
 Identities = 18/23 (78%), Positives = 20/23 (86%)

Query: 19 CNTARGFGEDIKHLGNSISRAAS 41
          CNTARGFGEDI+HLG +I RAA 
Sbjct: 21 CNTARGFGEDIQHLGGAIERAAK 43


>UniRef50_A8G8T1 Entericidin EcnAB n=2 Tax=Serratia RepID=A8G8T1_SERP5
          Length = 42

 Score = 43.9 bits (102), Expect = 0.002,   Method: Compositional matrix adjust.
 Identities = 18/24 (75%), Positives = 21/24 (87%)

Query: 18 GCNTARGFGEDIKHLGNSISRAAS 41
          GCNT RGFGED++HLG +ISR AS
Sbjct: 19 GCNTFRGFGEDVQHLGGAISRTAS 42


>UniRef50_C9Y198 Entericidin B n=15 Tax=Enterobacteriaceae RepID=C9Y198_CROTZ
          Length = 48

 Score = 43.1 bits (100), Expect = 0.002,   Method: Compositional matrix adjust.
 Identities = 23/44 (52%), Positives = 30/44 (68%), Gaps = 3/44 (6%)

Query: 1  MMKRLIVLVLLA---STLLTGCNTARGFGEDIKHLGNSISRAAS 41
          M+K+ I  +  A   S+LLT CNT RG GEDI+  GN+IS AA+
Sbjct: 1  MVKKTIAALFSALVLSSLLTACNTTRGVGEDIQDGGNAISGAAT 44


>UniRef50_UPI0001826DD8 entericidin EcnAB n=1 Tax=Enterobacter cancerogenus ATCC 35316
          RepID=UPI0001826DD8
          Length = 59

 Score = 42.0 bits (97), Expect = 0.005,   Method: Compositional matrix adjust.
 Identities = 24/44 (54%), Positives = 31/44 (70%), Gaps = 3/44 (6%)

Query: 1  MMKRLIVLVLLASTLLT---GCNTARGFGEDIKHLGNSISRAAS 41
          MMKR++ ++LL +       GCNTARG GEDI+ LG+ IS AAS
Sbjct: 16 MMKRIVKILLLLALSSAFLSGCNTARGMGEDIQDLGHIISHAAS 59


>UniRef50_B5WFA4 Entericidin EcnAB n=2 Tax=Burkholderia RepID=B5WFA4_9BURK
          Length = 45

 Score = 40.8 bits (94), Expect = 0.013,   Method: Compositional matrix adjust.
 Identities = 23/42 (54%), Positives = 28/42 (66%), Gaps = 2/42 (4%)

Query: 2  MKRLIVLVLLAST--LLTGCNTARGFGEDIKHLGNSISRAAS 41
          M RLI L++LA T  L+TGCNT  G GEDI   G++IS  A 
Sbjct: 1  MTRLIALLVLAGTAMLVTGCNTIAGAGEDISKGGHAISNEAE 42


>UniRef50_D0S831 Predicted protein n=3 Tax=Acinetobacter RepID=D0S831_ACIJO
          Length = 58

 Score = 39.3 bits (90), Expect = 0.035,   Method: Compositional matrix adjust.
 Identities = 16/40 (40%), Positives = 27/40 (67%)

Query: 2  MKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS 41
          MK++++  L+   +LTGCNT +G G+D+   GN++S  A 
Sbjct: 11 MKKIVLSSLVMLFVLTGCNTVKGMGKDVSSAGNAVSHTAE 50


>UniRef50_D1RI74 Conserved domain protein n=1 Tax=Legionella longbeachae D-4968
          RepID=D1RI74_LEGLO
          Length = 45

 Score = 39.3 bits (90), Expect = 0.039,   Method: Compositional matrix adjust.
 Identities = 16/43 (37%), Positives = 30/43 (69%), Gaps = 2/43 (4%)

Query: 1  MMKRLIVLVLLASTL--LTGCNTARGFGEDIKHLGNSISRAAS 41
          ++K+++++ ++ +TL  L+ C T  GFG+D+ H+G  I RAA 
Sbjct: 3  ILKKIVMMGIVLTTLGFLSACGTVNGFGKDVSHVGKDIQRAAQ 45


>UniRef50_Q6FE13 Bacteriolytic lipoprotein entericidin B n=3 Tax=Proteobacteria
          RepID=Q6FE13_ACIAD
          Length = 47

 Score = 39.3 bits (90), Expect = 0.043,   Method: Compositional matrix adjust.
 Identities = 14/41 (34%), Positives = 28/41 (68%)

Query: 1  MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS 41
          MMK+++   ++ + +LTGCNT +G G+D+   G++++  A 
Sbjct: 1  MMKKILAASVMVAFVLTGCNTFKGVGKDVSRAGDAVTNTAE 41


>UniRef50_C1DD60 Putative uncharacterized protein n=1 Tax=Laribacter hongkongensis
          HLHK9 RepID=C1DD60_LARHH
          Length = 45

 Score = 38.9 bits (89), Expect = 0.052,   Method: Compositional matrix adjust.
 Identities = 20/39 (51%), Positives = 27/39 (69%), Gaps = 1/39 (2%)

Query: 2  MKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAA 40
          MK LI  +LLAS LLT CNT  G G+D+   G++++ AA
Sbjct: 1  MKTLIG-ILLASCLLTACNTMAGMGQDVSSAGHAVTNAA 38


>UniRef50_B2T9N9 Entericidin EcnAB n=6 Tax=Burkholderia RepID=B2T9N9_BURPP
          Length = 52

 Score = 38.9 bits (89), Expect = 0.057,   Method: Compositional matrix adjust.
 Identities = 16/23 (69%), Positives = 17/23 (73%)

Query: 19 CNTARGFGEDIKHLGNSISRAAS 41
          CNT  GFGED+ HLGNSIS  A 
Sbjct: 29 CNTVHGFGEDMSHLGNSISNHAD 51


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P0ADB6 Entericidin A n=91 Tax=Enterobacteriaceae RepID=...    52   4e-06
UniRef50_B0VRY4 Bacteriolytic lipoprotein entericidin B n=11 Tax...    47   2e-04

Sequences not found previously or not previously below threshold:

UniRef50_Q6FE13 Bacteriolytic lipoprotein entericidin B n=3 Tax=...    43   0.002
UniRef50_D0S831 Predicted protein n=3 Tax=Acinetobacter RepID=D0...    41   0.014
UniRef50_B9XKK9 Entericidin EcnAB n=1 Tax=bacterium Ellin514 Rep...    40   0.027
UniRef50_C9Y199 Putative uncharacterized protein n=2 Tax=Cronoba...    39   0.046
UniRef50_A8G8T1 Entericidin EcnAB n=2 Tax=Serratia RepID=A8G8T1_...    38   0.079


CONVERGED!
>UniRef50_P0ADB6 Entericidin A n=91 Tax=Enterobacteriaceae RepID=ECNA_ECO57
          Length = 41

 Score = 52.3 bits (124), Expect = 4e-06,   Method: Composition-based stats.
 Identities = 41/41 (100%), Positives = 41/41 (100%)

Query: 1  MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS 41
          MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS
Sbjct: 1  MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS 41


>UniRef50_B0VRY4 Bacteriolytic lipoprotein entericidin B n=11 Tax=Moraxellaceae
          RepID=B0VRY4_ACIBS
          Length = 47

 Score = 46.9 bits (110), Expect = 2e-04,   Method: Composition-based stats.
 Identities = 16/41 (39%), Positives = 30/41 (73%)

Query: 1  MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS 41
          MMK+++V  ++ + +LTGCNT +GFG+D+   G++++  A 
Sbjct: 1  MMKKVLVASVMVAFVLTGCNTLKGFGQDVSKAGDAVTNTAQ 41


>UniRef50_Q6FE13 Bacteriolytic lipoprotein entericidin B n=3 Tax=Proteobacteria
          RepID=Q6FE13_ACIAD
          Length = 47

 Score = 43.4 bits (101), Expect = 0.002,   Method: Composition-based stats.
 Identities = 14/41 (34%), Positives = 28/41 (68%)

Query: 1  MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS 41
          MMK+++   ++ + +LTGCNT +G G+D+   G++++  A 
Sbjct: 1  MMKKILAASVMVAFVLTGCNTFKGVGKDVSRAGDAVTNTAE 41


>UniRef50_D0S831 Predicted protein n=3 Tax=Acinetobacter RepID=D0S831_ACIJO
          Length = 58

 Score = 40.8 bits (94), Expect = 0.014,   Method: Composition-based stats.
 Identities = 16/40 (40%), Positives = 27/40 (67%)

Query: 2  MKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS 41
          MK++++  L+   +LTGCNT +G G+D+   GN++S  A 
Sbjct: 11 MKKIVLSSLVMLFVLTGCNTVKGMGKDVSSAGNAVSHTAE 50


>UniRef50_B9XKK9 Entericidin EcnAB n=1 Tax=bacterium Ellin514 RepID=B9XKK9_9BACT
          Length = 44

 Score = 40.0 bits (92), Expect = 0.027,   Method: Composition-based stats.
 Identities = 21/41 (51%), Positives = 31/41 (75%), Gaps = 4/41 (9%)

Query: 2  MKRLIVLVLLASTLL----TGCNTARGFGEDIKHLGNSISR 38
          MK  +VL+L+A++LL    TGCNTA GFG+D+ + G++I R
Sbjct: 1  MKTRLVLILIATSLLVMAGTGCNTAHGFGKDVSNTGDAIQR 41


>UniRef50_C9Y199 Putative uncharacterized protein n=2 Tax=Cronobacter
          RepID=C9Y199_CROTZ
          Length = 43

 Score = 39.2 bits (90), Expect = 0.046,   Method: Composition-based stats.
 Identities = 18/24 (75%), Positives = 20/24 (83%)

Query: 18 GCNTARGFGEDIKHLGNSISRAAS 41
           CNTARGFGEDI+HLG +I RAA 
Sbjct: 20 ACNTARGFGEDIQHLGGAIERAAK 43


>UniRef50_A8G8T1 Entericidin EcnAB n=2 Tax=Serratia RepID=A8G8T1_SERP5
          Length = 42

 Score = 38.4 bits (88), Expect = 0.079,   Method: Composition-based stats.
 Identities = 18/24 (75%), Positives = 21/24 (87%)

Query: 18 GCNTARGFGEDIKHLGNSISRAAS 41
          GCNT RGFGED++HLG +ISR AS
Sbjct: 19 GCNTFRGFGEDVQHLGGAISRTAS 42


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.329    0.140    0.383 

Lambda     K      H
   0.267   0.0430    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 121,923,421
Number of Sequences: 3077464
Number of extensions: 2073761
Number of successful extensions: 14081
Number of sequences better than 1.0e-01: 54
Number of HSP's better than  0.1 without gapping: 56
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 14025
Number of HSP's gapped (non-prelim): 70
length of query: 41
length of database: 1,040,396,356
effective HSP length: 15
effective length of query: 26
effective length of database: 994,234,396
effective search space: 25850094296
effective search space used: 25850094296
T: 11
A: 40
X1: 16 ( 7.6 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 87 (38.1 bits)
