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["Feature List"]
[]
["F1","Disulphide bond breakage  ","The substitution breaks a disulphide bond that was in the wild-type. The maximum S-S length for the bond is 3.3 Å."]
["F2","Buried Pro introduced","The substitution introduces a buried proline."]
["F3","Clash","The mutant structure has a MolProbity clash score ≥ 30 and the increase in clash score is > 18 compared to the wild type."]
["F4","Buried hydrophilic introduced","The substitution replaces a buried hydrophobic residue with a hydrophilic residue."]
["F5","Buried charge introduced","The substitution replaces a buried uncharged residue with a charged residue."]
["F6","Secondary structure altered","A substitution results in a change in the DSSP secondary structure assignment at the variant position."]
["F7","Buried charge switch  ","The substitution switches the charge (+/-) of the buried residue buried."]
["F8","Disallowed phi/psi","The mutant residue is in outlier region while the wild-type residue in both the wild type and the repacked wild type are in the favoured or allowed regions."]
["F9","Buried charge replaced","The substitution replaces a buried charged residue with an uncharged residue."]
["F10","Buried Gly replaced","The substitution replaces a buried glycine."]
["F11","Buried H-bond breakage","The substitution breaks all side-chain / side-chain H-bond(s) and/or side-chain /main-chain bond(s) formed by the wild type which was buried. The maximum H-bond N-O length is 3.9 Å."]
["F12","Buried salt bridge breakage ","The substitution breaks a salt bridge formed by wild-type which was buried. The maximum N-O bond length is 5.0 Å."]
["F13","Cavity altered","The substitution leads to an expansion or contraction of the cavity volume of  ≥ 70 Å3. Cavity also refers to a pocket on the surface."]
["F14","Buried / exposed switch","The substitution results in a change between buried and exposed state of the target variant residue. (RSA < 9% for buried and the difference between RSA has to be at least 5%.)"]
["F15","Cis Pro replaced","A cis proline in the wild type is replaced in the mutant. "]
["F16","Gly in a bend","The wild-type residue is glycine and is located in a bend curvature (reported 'S' in DSSP)."]

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