Job Description Undefined Confidence 99.78% Date Thu Nov 13 08:26:15 GMT 2025 Rank 348 Aligned Residues 247 % Identity 13% Template c8yfyA_ PDB info PDB header: hydrolase Chain: A: PDB Molecule: Carboxylesterase PDBTitle: crystal structure of the est1 h274d mutant at ph 4.2 PDB Entry: PDBe RCSB PDBj Resolution UNK

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		19	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.				.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.				.	.	.	.	.	.	.	70	.	.
Predicted Secondary structure																										.	.	.																					.	.	.
Query Sequence		A	P	G	G	R	A	S	A	A	P	Q	V	H	Y	R	V	E	Q	V	G	D	V	K	V	.	.	.	F	Y	R	E	A	G	D	P	A	A	P	A	V	L	L	L	H	G	F	A	.	.	.	A	S	S	Y	M	W	R	D	V	I
Query Conservation																										.	.	.																					.	.	.
Alig confidence																										.	.	.																					.	.	.
Template Conservation
Template Sequence		A	S	A	A	P	K	A	E	V	R	K	V	E	D	I	K	I	P	G	S	E	T	S	I	N	A	R	V	Y	F	P	K	A	K	G	P	Y	G	V	L	V	Y	L	H	G	G	G	F	V	I	G	D	V	E	S	Y	D	P	L	C
Template Predicted Secondary structure
		73	.	.	.	.			.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.		.	.	.	.	.	.	.	.
Predicted Secondary structure							.	.																																													.
Query Sequence		E	A	L	A	D	.	.	G	Y	H	V	I	A	P	D	L	P	A	F	G	Y	T	E	A	P	A	R	G	Q	Y	D	Y	T	F	A	N	L	T	K	T	I	E	R	F	T	D	Q	L	K	L	T	.	R	Y	A	M	A	V	H	D
Query Conservation							.	.																																													.
Alig confidence							.	.																.	.	.																											.
Template Conservation																								.	.	.
Template Sequence		R	A	I	T	N	A	C	N	C	V	V	V	S	V	D	Y	R	L	A	P	E	Y	.	.	.	K	F	P	S	A	V	I	D	S	F	D	A	T	N	W	I	Y	N	N	L	D	K	F	D	G	E	M	G	I	A	I	A	G	D	S
Template Predicted Secondary structure																								.	.	.
		130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.			.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.
Predicted Secondary structure																		.	.
Query Sequence		Y	G	A	P	V	G	W	R	L	A	L	A	H	P	S	R	.	.	I	T	A	I	I	S	Q	N	G	N	A	Y	E	E	G	L	S	A	G	W	E	P	I	R	K	Y	W	N	D	P	T	A	E	N	R	Q	A	L	S	D	F	P
Query Conservation																		.	.
Alig confidence																		.	.																													.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation																																																.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		A	G	G	N	L	A	A	V	V	A	L	L	S	K	G	K	L	D	L	K	Y	Q	I	L	I	Y	P	A	V	G	F	D	S	V	S	R	S	M	I	E	Y	S	D	G	F	F	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Predicted Secondary structure																																																.	.	.	.	.	.	.	.	.	.	.	.	.	.
		188	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.
Predicted Secondary structure
Query Sequence		T	P	A	S	I	K	W	Q	Y	V	E	G	V	S	D	T	S	L	V	A	P	D	A	Y	T	L	E	G	A	Q	I	L	R	P	G	M	A	D	I	Q	L	D	L	L	L	D	Y	A	T	N	V	E	Q	Y	P	A	F	Q	A	Y
Query Conservation
Alig confidence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	L	T	R	E	H	I	E	W	F	G	S	Q	Y	L	R	S	P	A	D	L	L	D	F	R	F	S	P	I	I
Template Predicted Secondary structure		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
		248	.	250	.	.	.			.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270			.	.	.	.	.	.	.	.	.	280	.	.	.		.	.	.	.	.	.	290						.	.	.	.	.	.	.
Predicted Secondary structure								.	.																		.	.														.								.	.	.	.	.
Query Sequence		F	R	E	Y	Q	P	.	.	P	L	L	A	V	W	G	E	H	D	P	F	F	L	P	P	G	.	.	A	E	A	W	K	R	D	I	P	D	A	D	I	.	R	L	Y	D	T	G	H	.	.	.	.	.	F	A	L	E	T	H	S
Query Conservation								.	.																		.	.														.								.	.	.	.	.
Alig confidence								.	.																		.	.														.								.	.	.	.	.
Template Conservation
Template Sequence		A	Q	D	L	S	G	L	P	P	A	L	I	I	T	A	E	Y	D	P	L	R	D	Q	G	E	A	Y	A	N	R	L	L	Q	A	G	V	P	V	T	S	V	R	F	N	N	V	I	D	G	F	L	S	F	F	P	L	I	D	Q	G
Template Predicted Secondary structure
		298	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.
Predicted Secondary structure
Query Sequence		E	V	I	I	P	T	I	R	Q	F	L	D	D	H	V	S
Query Conservation
Alig confidence
Template Conservation
Template Sequence		K	D	A	I	G	L	I	G	S	V	L	R	R	T	F	Y
Template Predicted Secondary structure

No model constructed - rank, confidence too low

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Powell HR et al. Journal of Molecular Biology (2025) in press DOI: https://doi.org/10.1016/j.jmb.2025.168960
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