Job Description Undefined Confidence 99.83% Date Thu Nov 13 08:26:15 GMT 2025 Rank 229 Aligned Residues 218 % Identity 17% Template c8otuB_ PDB info PDB header: hydrolase Chain: B: PDB Molecule: Dienelactone hydrolase domain-containing protein PDBTitle: the crystal structure of pet44, a petase enzyme from alkalilimnicola ehrlichii PDB Entry: PDBe RCSB PDBj Resolution UNK

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		8	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.
Predicted Secondary structure
Query Sequence		T	V	S	A	S	E	T	R	G	D	H	A	P	G	G	R	A	S	A	A	P	Q	V	H	Y	R	V	E	Q	V	G	D	V	K	V	F	Y	R	E	A	G	D	P	A	A	P	A	V	L	L	L	H	G	F	A	A	S	S	Y	M
Query Conservation
Alig confidence
Template Conservation
Template Sequence		G	P	D	P	T	E	S	F	L	E	A	D	S	G	P	Y	T	I	A	T	S	N	V	S	S	L	V	R	G	F	G	G	G	T	I	Y	Y	P	V	N	A	E	G	T	M	A	A	I	V	V	I	P	G	Y	M	S	Y	Q	S	S
Template Predicted Secondary structure
		68	.	70	.	.	.	.	.	.	.		.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.
Predicted Secondary structure												.																																										.	.	.	.	.	.	.	.
Query Sequence		W	R	D	V	I	E	A	L	A	D	.	G	Y	H	V	I	A	P	D	L	P	A	F	G	Y	T	E	A	P	A	R	G	Q	Y	D	Y	T	F	A	N	L	T	K	T	I	E	R	F	T	D	Q	L	.	.	.	.	.	.	.	.
Query Conservation												.																																										.	.	.	.	.	.	.	.
Alig confidence												.																	.	.	.	.	.	.	.																			.	.	.	.	.	.	.	.
Template Conservation																													.	.	.	.	.	.	.
Template Sequence		I	S	F	W	G	P	R	L	A	S	H	G	F	V	V	M	T	I	D	T	N	R	I	S	D	Q	P	.	.	.	.	.	.	.	P	S	R	R	D	Q	I	E	A	A	L	E	Y	L	V	D	Q	S	N	S	S	R	S	P	I	N
Template Predicted Secondary structure																													.	.	.	.	.	.	.
					119	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.
Predicted Secondary structure		.	.	.
Query Sequence		.	.	.	K	L	T	R	Y	A	M	A	V	H	D	Y	G	A	P	V	G	W	R	L	A	L	A	H	P	S	R	I	T	A	I	I	S	Q	N	G	N	A	Y	E	E	G	L	S	A	G	W	E	P	I	R	K	Y	W	N	D	P
Query Conservation		.	.	.
Alig confidence		.	.	.																									.	.															.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation																													.	.															.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		G	M	V	D	P	N	R	L	G	A	V	G	W	S	M	G	G	G	G	T	L	R	L	A	A	D	D	.	.	G	I	Q	A	A	I	G	L	A	P	W	N	T	S	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Predicted Secondary structure																													.	.															.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
		176	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.
Predicted Secondary structure
Query Sequence		T	A	E	N	R	Q	A	L	S	D	F	P	T	P	A	S	I	K	W	Q	Y	V	E	G	V	S	D	T	S	L	V	A	P	D	A	Y	T	L	E	G	A	Q	I	L	R	P	G	M	A	D	I	Q	L	D	L	L	L	D	Y	A
Query Conservation
Template Conservation		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Predicted Secondary structure		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
		236	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270		.	.	.	.	.	.	.	.	.	280					.	.	.	.	.	.	.	.	.	290
Predicted Secondary structure																																					.											.	.	.	.
Query Sequence		T	N	V	E	Q	Y	P	A	F	Q	A	Y	F	R	E	Y	Q	P	P	L	L	A	V	W	G	E	H	D	P	F	F	L	P	P	G	.	A	E	A	W	K	R	D	I	P	D	.	.	.	.	A	D	I	R	L	Y	D	T	G	H
Query Conservation																																					.											.	.	.	.
Alig confidence		.	.	.	.	.	.	.	.	.																											.											.	.	.	.
Template Conservation		.	.	.	.	.	.	.	.	.
Template Sequence		.	.	.	.	.	.	.	.	.	S	L	G	F	R	S	I	E	T	P	T	L	I	F	A	C	E	R	D	S	I	A	P	V	R	S	H	A	S	P	F	Y	N	A	I	P	S	S	T	D	K	A	Y	F	E	I	S	G	G	N	H
Template Predicted Secondary structure		.	.	.	.	.	.	.	.	.
		291	.	.	.	.	.	.				.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320
Predicted Secondary structure									.	.	.
Query Sequence		F	A	L	E	T	H	S	.	.	.	E	V	I	I	P	T	I	R	Q	F	L	D	D	H	V	S	L	H	R	Q	E	G	A
Query Conservation									.	.	.
Alig confidence									.	.	.
Template Conservation
Template Sequence		Y	C	A	N	G	S	N	R	Y	D	A	L	L	G	K	Y	G	V	A	W	M	K	L	H	L	D	Q	D	Q	R	Y	A	P
Template Predicted Secondary structure

No model constructed - rank, confidence too low

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Powell HR et al. Journal of Molecular Biology (2025) in press DOI: https://doi.org/10.1016/j.jmb.2025.168960
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