Job Description Undefined Confidence 99.91% Date Thu Nov 13 08:26:15 GMT 2025 Rank 408 Aligned Residues 242 % Identity 12% Template c7z2uA_ PDB info PDB header: hydrolase Chain: A: PDB Molecule: Ferulic acid esterase PDBTitle: wild-type ferulic acid esterase from lactobacillus buchneri in complex with ferulate PDB Entry: PDBe RCSB PDBj Resolution UNK

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		29	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50						.	.	.	.	.	.	.	.	.	60	.	.	.	.	.					.	.	.	.	70	.	.	.	.	.	.	.		.
Predicted Secondary structure																								.	.	.	.	.																.	.	.	.													.
Query Sequence		Q	V	H	Y	R	V	E	Q	V	G	D	V	K	V	F	Y	R	E	A	G	D	P	.	.	.	.	.	A	A	P	A	V	L	L	L	H	G	F	A	A	S	S	.	.	.	.	Y	M	W	R	D	V	I	E	A	L	A	D	.	G
Query Conservation																								.	.	.	.	.																.	.	.	.													.
Alig confidence																								.	.	.	.	.																.	.	.	.													.
Template Conservation
Template Sequence		M	I	K	F	V	T	T	E	I	N	G	L	T	L	R	G	T	A	H	V	P	D	G	E	P	G	Q	Q	F	P	T	V	L	M	F	H	G	F	G	A	V	R	D	E	G	F	R	L	F	I	Q	M	S	N	R	L	M	E	N	G
Template Known Secondary structure											T	T										.	S	B	S	T	T												T	T			T	T		G	G	G											T	T	T
Template Predicted Secondary structure
		79	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120				.	.	.	.	.	.	.	.	.	130	.	.	.	.	.
Predicted Secondary structure																																												.	.	.
Query Sequence		Y	H	V	I	A	P	D	L	P	A	F	G	Y	T	E	A	P	A	R	G	Q	Y	D	Y	T	F	A	N	L	T	K	T	I	E	R	F	T	D	Q	L	K	L	.	.	.	T	R	Y	A	M	A	V	H	D	Y	G	A	P	V	G
Query Conservation																																												.	.	.
Alig confidence																																												.	.	.
Template Conservation
Template Sequence		I	A	A	V	R	F	D	F	G	C	H	G	E	S	D	G	E	F	E	D	F	T	F	S	Q	E	L	N	E	G	S	A	L	I	D	A	V	K	S	M	S	F	V	D	S	T	K	F	S	L	L	G	E	S	L	G	S	V	A	A
Template Known Secondary structure								.	.	T	T	S	T	T	S	S	S		G	G	G																			T	S	T	T												T
Template Predicted Secondary structure
		136	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.		.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.
Predicted Secondary structure																													.
Query Sequence		W	R	L	A	L	A	H	P	S	R	I	T	A	I	I	S	Q	N	G	N	A	Y	E	E	G	L	S	.	A	G	W	E	P	I	R	K	Y	W	N	D	P	T	A	E	N	R	Q	A	L	S	D	F	P	T	P	A	S	I	K	W
Query Conservation																													.
Alig confidence																													.
Template Conservation
Template Sequence		S	I	V	A	G	K	R	S	T	E	L	T	S	L	C	M	W	S	P	A	A	S	F	L	D	E	I	L	N	D	H	T	L	Q	G	K	T	V	D	N	V	E	K	D	G	Y	F	D	F	Y	G	L	K	L	G	K	A	F	F	D
Template Known Secondary structure								T	T	T	S		S						S			T	T												T	T				T	T					S					T	T
Template Predicted Secondary structure
		195	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.
Predicted Secondary structure
Query Sequence		Q	Y	V	E	G	V	S	D	T	S	L	V	A	P	D	A	Y	T	L	E	G	A	Q	I	L	R	P	G	M	A	D	I	Q	L	D	L	L	L	D	Y	A	T	N	V	E	Q	Y	P	A	F	Q	A	Y	F	R	E	Y	Q	P	P
Query Conservation
Alig confidence			.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation			.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		D	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	L	K	N	I	N	I	F	D	N	A	K	K	Y	Q	G	P
Template Known Secondary structure			.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	G	G	G			S					T	T			S
Template Predicted Secondary structure			.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
		255	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.		.	.	.	.	.	290	.	.	.		.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.
Predicted Secondary structure																																.										.
Query Sequence		L	L	A	V	W	G	E	H	D	P	F	F	L	P	P	G	A	E	A	W	K	R	D	I	P	D	A	D	I	R	.	L	Y	D	T	G	H	F	A	L	.	E	T	H	S	E	V	I	I	P	T	I	R	Q	F	L	D	D	H	V
Query Conservation																																.										.
Alig confidence											.																					.										.
Template Conservation											.
Template Sequence		V	K	I	V	Y	G	T	N	D	.	F	I	P	E	K	Y	S	H	K	Y	M	D	G	Y	E	N	G	E	L	V	I	V	Q	D	G	D	H	G	W	Q	S	V	P	S	R	K	R	I	L	D	E	T	M	K	F	F	R	K	T	L
Template Known Secondary structure								T	T		.	S	S		T											T	T							T	T			T	T			S
Template Predicted Secondary structure											.
		313	.
Predicted Secondary structure
Query Sequence		S	L
Query Conservation
Alig confidence
Template Conservation
Template Sequence		L	E
Template Known Secondary structure
Template Predicted Secondary structure

No model constructed - rank, confidence too low

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Powell HR et al. Journal of Molecular Biology (2025) in press DOI: https://doi.org/10.1016/j.jmb.2025.168960
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