Job Description Undefined Confidence 99.85% Date Thu Nov 13 08:26:15 GMT 2025 Rank 301 Aligned Residues 226 % Identity 15% Template c5lukA_ PDB info PDB header: hydrolase Chain: A: PDB Molecule: Cutinase 2 PDBTitle: structure of a double variant of cutinase 2 from thermobifida cellulosilytica PDB Entry: PDBe RCSB PDBj Resolution UNK

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		4	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.			40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.
Predicted Secondary structure																																						.	.
Query Sequence		D	S	L	T	T	V	S	A	S	E	T	R	G	D	H	A	P	G	G	R	A	S	A	A	P	Q	V	H	Y	R	V	E	Q	V	G	D	.	.	V	K	V	F	Y	R	E	A	G	D	P	A	A	P	A	V	L	L	L	H	G	F
Query Conservation																																						.	.
Alig confidence																																						.	.
Template Conservation
Template Sequence		A	N	P	Y	E	R	G	P	N	P	T	D	A	L	L	E	A	R	S	G	P	F	S	V	S	E	E	N	V	S	R	F	G	A	D	G	F	G	G	G	T	I	Y	Y	P	R	E	N	N	T	Y	G	A	V	A	I	S	P	G	Y
Template Predicted Secondary structure
		62	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.		.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110		.	.	.	.	.	.	.	.	.
Predicted Secondary structure																		.																																		.
Query Sequence		A	A	S	S	Y	M	W	R	D	V	I	E	A	L	A	D	.	G	Y	H	V	I	A	P	D	L	P	A	F	G	Y	T	E	A	P	A	R	G	Q	Y	D	Y	T	F	A	N	L	T	K	T	.	I	E	R	F	T	D	Q	L	K
Query Conservation																		.																																		.
Alig confidence																		.																																		.
Template Conservation
Template Sequence		T	G	T	Q	A	S	V	A	W	L	G	E	R	I	A	S	H	G	F	V	V	I	T	I	D	T	N	T	T	L	D	Q	P	D	S	R	A	R	Q	L	N	A	A	L	D	Y	M	I	N	D	A	S	S	A	V	R	S	R	I	D
Template Predicted Secondary structure
		120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.
Predicted Secondary structure
Query Sequence		L	T	R	Y	A	M	A	V	H	D	Y	G	A	P	V	G	W	R	L	A	L	A	H	P	S	R	I	T	A	I	I	S	Q	N	G	N	A	Y	E	E	G	L	S	A	G	W	E	P	I	R	K	Y	W	N	D	P	T	A	E	N
Query Conservation
Alig confidence																											.													.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation																											.													.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		S	S	R	L	A	V	M	G	H	S	M	G	G	G	G	T	L	R	L	A	S	Q	R	P	D	.	L	K	A	A	I	P	L	T	P	W	H	L	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Predicted Secondary structure																											.													.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
		180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.
Predicted Secondary structure
Query Sequence		R	Q	A	L	S	D	F	P	T	P	A	S	I	K	W	Q	Y	V	E	G	V	S	D	T	S	L	V	A	P	D	A	Y	T	L	E	G	A	Q	I	L	R	P	G	M	A	D	I	Q	L	D	L	L	L	D	Y	A	T	N	V	E
Query Conservation
Template Conservation		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Predicted Secondary structure		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
		240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.		.	270	.	.	.	.	.	.	.	.	.	280				.	.	.		.	.	.	.	.	.	290	.	.	.	.
Predicted Secondary structure																															.													.	.	.				.
Query Sequence		Q	Y	P	A	F	Q	A	Y	F	R	E	Y	Q	P	P	L	L	A	V	W	G	E	H	D	P	F	F	L	P	.	P	G	A	E	A	W	K	R	D	I	P	D	.	.	.	A	D	I	.	R	L	Y	D	T	G	H	F	A	L	E
Query Conservation																															.													.	.	.				.
Alig confidence		.	.	.	.	.																									.													.	.	.				.
Template Conservation		.	.	.	.	.
Template Sequence		.	.	.	.	.	N	K	N	W	S	S	V	R	V	P	T	L	I	I	G	A	D	L	D	T	I	A	P	V	L	T	H	A	R	P	F	Y	N	S	L	P	T	S	I	S	K	A	Y	L	E	L	D	G	A	T	H	F	A	P	N
Template Predicted Secondary structure		.	.	.	.	.
		295	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.
Predicted Secondary structure
Query Sequence		T	H	S	E	V	I	I	P	T	I	R	Q	F	L	D	D	H	V	S	L	H	R	Q
Query Conservation
Alig confidence
Template Conservation
Template Sequence		I	P	N	K	I	I	G	K	Y	S	V	A	W	L	K	R	F	V	D	N	D	T	R
Template Predicted Secondary structure

No model constructed - rank, confidence too low

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Powell HR et al. Journal of Molecular Biology (2025) in press DOI: https://doi.org/10.1016/j.jmb.2025.168960
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