Job Description Undefined Confidence 99.83% Date Thu Nov 13 08:26:15 GMT 2025 Rank 318 Aligned Residues 236 % Identity 14% Template c5l8sD_ PDB info PDB header: hydrolase Chain: D: PDB Molecule: Amino acyl peptidase PDBTitle: ;the crystal structure of a cold-adapted acylaminoacyl peptidase reveals a novel quaternary architecture based on the arm-exchange mechanism; PDB Entry: PDBe RCSB PDBj Resolution UNK

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		27	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.					.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.			.	.	70	.	.	.	.	.	.	.		.	.
Predicted Secondary structure																							.	.	.	.																					.	.											.
Query Sequence		A	P	Q	V	H	Y	R	V	E	Q	V	G	D	V	K	V	F	Y	R	E	A	.	.	.	.	G	D	P	A	A	P	A	V	L	L	L	H	G	F	A	A	S	S	Y	M	.	.	W	R	D	V	I	E	A	L	A	D	.	G	Y
Query Conservation																							.	.	.	.																					.	.											.
Alig confidence																							.	.	.	.																					.	.											.
Template Conservation
Template Sequence		V	E	P	D	I	V	S	Y	T	S	F	D	G	M	E	I	E	A	L	L	F	K	A	K	P	E	N	D	N	G	Y	T	I	F	W	P	H	G	G	P	Q	S	A	E	R	K	M	F	R	S	M	F	Q	C	F	I	N	R	G	Y
Template Known Secondary structure			.	.								T	T	S									.	.	.	T	T	T			S								S		S	S	S				S													T
Template Predicted Secondary structure
		80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.						.	.	.	.	110	.	.	.	.	.	.	.	.			.	120	.	.	.	.	.	.	.	.	.	130	.	.
Predicted Secondary structure																												.	.	.	.	.														.	.
Query Sequence		H	V	I	A	P	D	L	P	A	F	G	Y	T	E	A	P	A	R	G	Q	Y	D	Y	T	F	A	.	.	.	.	.	N	L	T	K	T	I	E	R	F	T	D	Q	L	.	.	K	L	T	R	Y	A	M	A	V	H	D	Y	G	A
Query Conservation																												.	.	.	.	.														.	.
Alig confidence																												.	.	.	.	.														.	.
Template Conservation
Template Sequence		T	I	F	A	P	N	F	R	G	S	T	G	Y	G	S	A	F	T	K	L	V	E	L	D	W	G	E	G	P	R	L	D	C	I	A	G	I	E	W	L	F	E	S	G	F	T	D	R	N	K	L	F	L	V	G	G	S	Y	G	G
Template Known Secondary structure									T	T		S	S	S						G	G	G	T	T		S	S	S																	T	S	S		T	T								T
Template Predicted Secondary structure
		133	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.
Predicted Secondary structure
Query Sequence		P	V	G	W	R	L	A	L	A	H	P	S	R	I	T	A	I	I	S	Q	N	G	N	A	Y	E	E	G	L	S	A	G	W	E	P	I	R	K	Y	W	N	D	P	T	A	E	N	R	Q	A	L	S	D	F	P	T	P	A	S	I
Query Conservation
Alig confidence
Template Conservation
Template Sequence		Y	M	A	L	L	L	H	G	R	H	S	D	Y	F	R	A	V	V	D	I	F	G	P	S	D	L	F	T	F	I	N	S	V	P	P	H	W	K	P	I	M	E	R	W	L	G	D	P	E	R	D	K	E	R	F	I	K	D	S	P
Template Known Secondary structure												G	G	G		S						S												S		G	G	G								T			T	T	T	T							T		G
Template Predicted Secondary structure
		193	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.
Predicted Secondary structure
Query Sequence		K	W	Q	Y	V	E	G	V	S	D	T	S	L	V	A	P	D	A	Y	T	L	E	G	A	Q	I	L	R	P	G	M	A	D	I	Q	L	D	L	L	L	D	Y	A	T	N	V	E	Q	Y	P	A	F	Q	A	Y	F	R	E	Y	Q
Query Conservation
Alig confidence					.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation					.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		V	T	Y	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	L	D	G	M	V
Template Known Secondary structure		G	G	G	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	G	G	G
Template Predicted Secondary structure					.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
		253	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.					280	.	.	.		.	.	.	.	.	.	290		.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.
Predicted Secondary structure																													.	.	.	.					.								.
Query Sequence		P	P	L	L	A	V	W	G	E	H	D	P	F	F	L	P	P	G	A	E	A	W	K	R	D	I	P	.	.	.	.	D	A	D	I	.	R	L	Y	D	T	G	H	.	F	A	L	E	T	H	S	E	V	I	I	P	T	I	R	Q
Query Conservation																													.	.	.	.					.								.
Alig confidence																													.	.	.	.					.								.
Template Conservation
Template Sequence		K	P	M	L	V	I	Q	G	A	K	D	P	R	V	V	K	E	E	S	D	Q	I	V	A	K	L	K	E	K	G	R	D	V	E	Y	L	V	L	E	D	E	G	H	G	F	S	K	K	E	N	E	I	K	V	Y	S	L	M	L	A
Template Known Secondary structure		S								T	T		S	S	S															T	T									T	T		S	S	S			S
Template Predicted Secondary structure
		307	.	.	310	.	.	.	.
Predicted Secondary structure
Query Sequence		F	L	D	D	H	V	S	L
Query Conservation
Alig confidence
Template Conservation
Template Sequence		F	L	E	K	H	Q	A	L
Template Known Secondary structure						T	S
Template Predicted Secondary structure

No model constructed - rank, confidence too low

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Powell HR et al. Journal of Molecular Biology (2025) in press DOI: https://doi.org/10.1016/j.jmb.2025.168960
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