Job Description Undefined Confidence 99.97% Date Thu Nov 13 08:26:15 GMT 2025 Rank 88 Aligned Residues 275 % Identity 18% Template c5jkjA_ PDB info PDB header: hydrolase Chain: A: PDB Molecule: Esterase E22 PDBTitle: crystal structure of esterase e22 l374d mutant PDB Entry: PDBe RCSB PDBj Resolution UNK

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		36	.	.	.	40	.	.	.	.	.	.	.	.	.				50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.														.	.	.	70	.	.				.	.	.
Predicted Secondary structure																.	.	.																		.	.	.	.	.	.	.	.	.	.	.	.	.							.	.	.				.
Query Sequence		Q	V	G	D	V	K	V	F	Y	R	E	A	G	D	.	.	.	P	A	A	P	A	V	L	L	L	H	G	F	A	A	S	S	Y	.	.	.	.	.	.	.	.	.	.	.	.	.	M	W	R	D	V	I	.	.	.	E	A	L	.
Query Conservation																.	.	.																		.	.	.	.	.	.	.	.	.	.	.	.	.							.	.	.				.
Alig confidence																.	.	.																		.	.	.	.	.	.	.	.	.	.	.	.	.							.	.	.				.
Template Conservation
Template Sequence		Q	I	K	N	V	K	V	G	W	E	A	Y	G	T	L	N	D	A	K	S	N	V	I	L	I	T	H	Y	F	S	G	S	S	H	A	A	G	K	Y	D	E	N	D	P	A	P	G	Y	W	D	S	I	I	G	P	G	K	A	I	D
Template Known Secondary structure														S				T	T											T	T			S			S	S		S	S	T	T		S	S			T	T	T	T	T			T	T	S	S
Template Predicted Secondary structure
		76	.	.	.	80	.	.	.	.	.	.	.	.					.	90	.				.	.	.	.	.	.	.	.	100	.												.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.
Predicted Secondary structure															.	.	.	.				.	.	.											.	.	.	.	.	.	.	.	.	.	.
Query Sequence		A	D	G	Y	H	V	I	A	P	D	L	P	A	.	.	.	.	F	G	Y	.	.	.	T	E	A	P	A	R	G	Q	Y	D	.	.	.	.	.	.	.	.	.	.	.	Y	T	F	A	N	L	T	K	T	I	E	R	F	T	D	Q
Query Conservation															.	.	.	.				.	.	.											.	.	.	.	.	.	.	.	.	.	.
Alig confidence															.	.	.	.				.	.	.											.	.	.	.	.	.	.	.	.	.	.
Template Conservation
Template Sequence		T	D	R	F	Y	V	I	S	V	D	T	L	A	N	L	N	A	Y	D	P	H	V	I	T	T	G	P	T	S	I	N	P	D	T	G	K	P	Y	G	L	D	F	P	V	V	T	I	R	D	F	V	N	V	Q	K	A	L	L	E	S
Template Known Secondary structure		T	T	T							.	.	T	T		S		T	T		S	S					S	T	T	S			T	T	T	T				G	G	G	S	.	.
Template Predicted Secondary structure
		118	.	120	.	.	.	.		.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.		.	160	.	.	.	.	.	.	.	.	.	170	.	.
Predicted Secondary structure									.																																			.															.	.	.
Query Sequence		L	K	L	T	R	Y	A	.	M	A	V	H	D	Y	G	A	P	V	G	W	R	L	A	L	A	H	P	S	R	I	T	A	I	I	S	Q	N	G	N	A	Y	E	.	E	G	L	S	A	G	W	E	P	I	R	K	Y	W	.	.	.
Query Conservation									.																																			.															.	.	.
Alig confidence									.																																			.															.	.	.
Template Conservation
Template Sequence		L	G	I	S	K	L	Y	A	V	I	G	P	S	M	G	S	M	Q	A	I	D	W	A	S	A	Y	P	G	W	V	E	R	M	I	S	V	I	G	A	G	Q	S	D	A	W	T	T	A	A	L	E	H	W	A	T	P	I	T	L	D
Template Known Secondary structure		T	T				B	S						T													S	G	G	G	B	S						S			S	B																			S
Template Predicted Secondary structure
												173	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.														.	.	.	.	.
Predicted Secondary structure		.	.	.	.	.	.	.	.	.	.																																	.	.	.	.	.	.	.	.	.	.	.	.	.
Query Sequence		.	.	.	.	.	.	.	.	.	.	N	D	P	T	A	E	N	R	Q	A	L	S	D	F	P	T	P	A	S	I	K	W	Q	Y	V	E	G	V	S	D	T	S	.	.	.	.	.	.	.	.	.	.	.	.	.	L	V	A	P	D
Query Conservation		.	.	.	.	.	.	.	.	.	.																																	.	.	.	.	.	.	.	.	.	.	.	.	.
Alig confidence		.	.	.	.	.	.	.	.	.	.																													.				.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation																																								.
Template Sequence		K	N	W	N	N	G	A	Y	S	K	E	Q	A	P	L	N	G	L	A	A	S	L	M	L	I	T	Q	N	A	L	T	P	S	F	F	N	Q	T	.	G	N	T	L	G	Y	K	N	V	E	S	A	P	L	N	D	I	R	Q	S	H
Template Known Secondary structure		G	G	G	G	G	G	T			G	G	G																	T	S									.					T			S	S								T	T	S
Template Predicted Secondary structure																																								.
		210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.	.	.	.						.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.
Predicted Secondary structure																																						.	.	.	.	.
Query Sequence		A	Y	T	L	E	G	A	Q	I	L	R	P	G	M	A	D	I	Q	L	D	L	L	L	D	Y	A	T	N	V	E	Q	Y	P	A	F	Q	.	.	.	.	.	A	Y	F	R	E	Y	Q	P	P	L	L	A	V	W	G	E	H	D	P
Query Conservation																																						.	.	.	.	.
Alig confidence																																						.	.	.	.	.
Template Conservation
Template Sequence		S	I	V	N	W	L	R	E	R	A	K	T	R	A	K	S	M	D	A	N	H	L	L	Y	L	V	R	A	C	Q	L	F	V	A	G	H	Q	G	N	L	E	Q	G	L	A	S	I	K	A	K	T	L	F	I	P	A	Q	T	D	L
Template Known Secondary structure																T	T															T		B	G	G	G	S	S	S							T			S							B	T	T		S
Template Predicted Secondary structure
		265	.	.	.	.	270	.	.	.	.	.	.	.	.					.	280	.	.	.	.		.	.		.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.
Predicted Secondary structure																.	.	.	.							.			.
Query Sequence		F	F	L	P	P	G	A	E	A	W	K	R	D	I	.	.	.	.	P	D	A	D	I	R	.	L	Y	.	D	T	G	H	F	A	L	E	T	H	S	E	V	I	I	P	T	I	R	Q	F	L	D	D	H
Query Conservation																.	.	.	.							.			.
Alig confidence																.	.	.	.							.			.
Template Conservation
Template Sequence		L	L	M	P	Y	L	S	Q	S	A	H	Q	G	L	T	S	M	N	N	D	S	T	L	V	T	L	N	G	K	L	G	H	D	E	G	V	T	N	V	S	A	Q	A	Q	A	I	R	Q	F	L	E	N	D
Template Known Secondary structure		S	S															T	T								B			T	T	G	G	G						G	G	G	G
Template Predicted Secondary structure

No model constructed - rank, confidence too low

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Please cite: Phyre2.2: A Community Resource for Template-based Protein Structure Prediction
Powell HR et al. Journal of Molecular Biology (2025) in press DOI: https://doi.org/10.1016/j.jmb.2025.168960
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