Job Description Undefined Confidence 99.97% Date Thu Nov 13 08:26:15 GMT 2025 Rank 325 Aligned Residues 272 % Identity 12% Template c5frdA_ PDB info PDB header: hydrolase Chain: A: PDB Molecule: CARBOXYLESTERASE (EST-2) PDBTitle: structure of a thermophilic esterase PDB Entry: PDBe RCSB PDBj Resolution UNK

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		29	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.					50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.				.	.	.		70	.	.	.	.	.	.	.	.	.	80
Predicted Secondary structure																							.	.	.	.																		.	.	.				.
Query Sequence		Q	V	H	Y	R	V	E	Q	V	G	D	V	K	V	F	Y	R	E	A	G	D	.	.	.	.	P	A	A	P	A	V	L	L	L	H	G	F	A	A	S	S	Y	.	.	.	M	W	R	.	D	V	I	E	A	L	A	D	G	Y	H
Query Conservation																							.	.	.	.																		.	.	.				.
Alig confidence																							.	.	.	.																		.	.	.				.							.	.	.
Template Conservation																																																									.	.	.
Template Sequence		R	V	F	I	D	D	V	D	D	G	V	K	K	V	S	L	L	K	K	G	R	R	R	E	E	E	R	R	K	K	V	F	Y	I	H	S	S	G	S	S	D	D	A	T	Q	Q	W	V	V	N	Q	L	T	A	I	.	.	.	I	G
Template Known Secondary structure							.		T	.	T			.						.		S	.	.	.	.	S		.	.	S							T	T		.	.		G	G	G	.	G		.						T	.	.	.	.
Template Predicted Secondary structure																																																									.	.	.
		81	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.			.	.	.	.	.	110	.	.	.				.	.	.	.	.	.		120	.	.	.	.	.	.	.	.	.	130	.	.		.
Predicted Secondary structure																										.	.										.	.	.							.														.
Query Sequence		V	I	A	P	D	L	P	A	F	G	Y	T	E	A	P	A	R	G	Q	Y	D	Y	T	F	.	.	A	N	L	T	K	T	I	E	R	.	.	.	F	T	D	Q	L	K	.	L	T	R	Y	A	M	A	V	H	D	Y	G	A	.	P
Query Conservation																										.	.										.	.	.							.														.
Alig confidence																					.					.	.										.	.	.							.														.
Template Conservation																					.
Template Sequence		G	Y	A	I	D	L	P	N	H	G	Q	Q	S	D	T	V	E	E	V	.	N	N	S	V	D	D	E	E	Y	A	Y	Y	A	S	S	S	E	S	L	K	K	K	K	T	V	G	K	K	A	V	V	V	G	H	S	L	L	G	G	A
Template Known Secondary structure								T	T	S	T	T	.	S	.	.			.		.		.	S			.	.								.	.						.	.		T	S	S	.							T		.
Template Predicted Secondary structure																					.
		134	.	.	.	.					.	140	.	.	.	.	.	.	.			.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.
Predicted Secondary structure							.	.	.	.										.	.
Query Sequence		V	G	W	R	L	.	.	.	.	A	L	A	H	P	S	R	I	T	.	.	A	I	I	S	Q	N	G	N	A	Y	E	E	G	L	S	A	G	W	E	P	I	R	K	Y	W	N	D	P	T	A	E	N	R	Q	A	L	S	D	F	P
Query Conservation							.	.	.	.										.	.
Alig confidence							.	.	.	.										.	.															.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation																																				.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		V	A	Q	K	K	L	Y	L	L	R	R	R	N	P	E	E	I	C	C	L	A	L	V	L	V	G	T	G	A	R	L	R	V	L	.	.	.	.	.	.	.	.	.	.	.	.	.	.	L	P	E	I	L	E	E	G	L	K	K	K
Template Known Secondary structure						.				.	.	.			G	G	.	G		.							S			S	B					.	.	.	.	.	.	.	.	.	.	.	.	.	.	.					T	.					.
Template Predicted Secondary structure																																				.	.	.	.	.	.	.	.	.	.	.	.	.	.
						188	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.			.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.		.	.	.	.	.	.	.	240
Predicted Secondary structure		.	.	.	.																				.	.																											.
Query Sequence		.	.	.	.	T	P	A	S	I	K	W	Q	Y	V	E	G	V	S	D	T	S	L	V	.	.	A	P	D	A	Y	T	L	E	G	A	Q	I	L	R	P	G	M	A	D	I	Q	L	D	L	L	L	.	D	Y	A	T	N	V	E	Q
Query Conservation		.	.	.	.																				.	.																											.
Alig confidence		.	.	.	.																				.	.																											.				.	.	.	.
Template Conservation																																																									.	.	.	.
Template Sequence		K	E	E	P	E	K	K	A	V	D	L	M	L	S	M	A	F	A	S	K	K	G	G	E	E	E	E	Y	E	E	K	K	K	K	R	R	E	F	L	D	R	V	D	V	V	L	H	L	D	L	S	S	L	C	D	.	.	.	.	R
Template Known Secondary structure		.	.					.								T	T		S	S		.	.	S			.	.			.	.	.									T				.							.				.	.	.	.	T
Template Predicted Secondary structure																																																									.	.	.	.
		241	.	.	.	.	.	.	.	.						250	.					.	.	.	.	.	.	.	.	260	.	.			.	.	.	.	.	.		.	270	.	.	.	.	.	.	.			.	.	280	.	.			.
Predicted Secondary structure											.	.	.	.	.			.	.	.	.												.	.							.										.	.						.	.		.
Query Sequence		Y	P	A	F	Q	A	Y	F	R	.	.	.	.	.	E	Y	.	.	.	.	Q	P	P	L	L	A	V	W	G	E	H	.	.	D	P	F	F	L	P	.	P	G	A	E	A	W	K	R	D	.	.	I	P	D	A	D	.	.	I	.
Query Conservation											.	.	.	.	.			.	.	.	.												.	.							.										.	.						.	.		.
Alig confidence											.	.	.	.	.			.	.	.	.												.	.							.										.	.						.	.		.
Template Conservation
Template Sequence		R	F	D	L	L	E	E	D	Y	R	R	N	G	K	K	L	K	I	I	G	G	V	P	T	L	V	I	V	G	E	E	E	D	K	K	L	T	P	L	K	K	Y	H	E	F	F	H	K	K	H	I	I	P	N	S	E	E	L	V	V
Template Known Secondary structure		.		B				.				.	T	T		.	S			.	.		S								T	T	.		S	.	S	S				.								.			.	T	T			.
Template Predicted Secondary structure
		284	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.
Predicted Secondary structure
Query Sequence		R	L	Y	D	T	G	H	F	A	L	E	T	H	S	E	V	I	I	P	T	I	R	Q	F	L	D	D	H	V	S	L	H	R	Q	E	G	A	H	A
Query Conservation
Alig confidence
Template Conservation
Template Sequence		I	I	P	G	A	S	H	M	V	M	L	E	K	H	V	E	F	N	E	A	L	E	E	K	K	F	L	K	K	K	K	V	V	G	V	A	E	E	V
Template Known Secondary structure			.	T	T			T	T					S										.	.						.	.	.		T				.
Template Predicted Secondary structure

No model constructed - rank, confidence too low

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Powell HR et al. Journal of Molecular Biology (2025) in press DOI: https://doi.org/10.1016/j.jmb.2025.168960
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