Job Description Undefined Confidence 99.73% Date Thu Nov 13 08:26:15 GMT 2025 Rank 424 Aligned Residues 205 % Identity 14% Template c4zrsA_ PDB info PDB header: hydrolase Chain: A: PDB Molecule: Esterase PDBTitle: crystal structure of a cloned feruloyl esterase from a soil metagenomic library PDB Entry: PDBe RCSB PDBj Resolution UNK

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		42	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.									.	.	.	.	.	.	.	70	.	.	.	.	.	.	.		.	.	80	.	.	.	.	.	.	.	.	.	90	.	.
Predicted Secondary structure																							.	.	.	.	.	.	.	.																.
Query Sequence		V	F	Y	R	E	A	G	D	P	A	A	P	A	V	L	L	L	H	G	F	A	.	.	.	.	.	.	.	.	A	S	S	Y	M	W	R	D	V	I	E	A	L	A	D	.	G	Y	H	V	I	A	P	D	L	P	A	F	G	Y	T
Query Conservation																							.	.	.	.	.	.	.	.																.
Alig confidence																							.	.	.	.	.	.	.	.																.
Template Conservation
Template Sequence		D	L	F	R	K	A	D	T	R	D	A	P	V	L	V	F	V	H	G	G	G	F	V	M	G	D	K	R	L	A	E	T	P	F	Y	D	N	I	G	V	F	A	A	Q	Q	G	F	V	G	V	T	I	T	Y	R	L	A	P	A	H
Template Known Secondary structure							T	T			S										S	T	T	T	S			S			T	T	S		T	T										T	T	S								.	.	T	T	T	S
Template Predicted Secondary structure
		93	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.						.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.
Predicted Secondary structure																											.	.	.	.	.																													.	.
Query Sequence		E	A	P	A	R	G	Q	Y	D	Y	T	F	A	N	L	T	K	T	I	E	R	F	T	D	Q	.	.	.	.	.	L	K	L	T	R	Y	A	M	A	V	H	D	Y	G	A	P	V	G	W	R	L	A	L	A	H	P	S	R	.	.
Query Conservation																											.	.	.	.	.																													.	.
Alig confidence						.	.	.	.	.	.																.	.	.	.	.																													.	.
Template Conservation						.	.	.	.	.	.
Template Sequence		Q	F	P	S	.	.	.	.	.	.	G	P	E	D	L	A	A	V	V	R	W	L	K	A	N	V	A	Q	Y	G	G	D	P	D	K	I	V	L	S	G	Q	A	G	A	A	H	V	A	S	Y	I	A	H	K	A	H	H	A	T	E
Template Known Secondary structure			T	T		.	.	.	.	.	.																G	G	G	G	T											S	S												G	G	G		S	S	G
Template Predicted Secondary structure						.	.	.	.	.	.
					146	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.
Predicted Secondary structure		.	.	.
Query Sequence		.	.	.	I	T	A	I	I	S	Q	N	G	N	A	Y	E	E	G	L	S	A	G	W	E	P	I	R	K	Y	W	N	D	P	T	A	E	N	R	Q	A	L	S	D	F	P	T	P	A	S	I	K	W	Q	Y	V	E	G	V	S	D
Query Conservation		.	.	.
Alig confidence		.	.	.																.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation																				.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		G	G	G	I	A	G	A	I	L	M	S	G	I	Y	D	T	L	T	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Known Secondary structure		G	G	S		S						S					T	T	T	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Predicted Secondary structure																				.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
		203	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.
Predicted Secondary structure
Query Sequence		T	S	L	V	A	P	D	A	Y	T	L	E	G	A	Q	I	L	R	P	G	M	A	D	I	Q	L	D	L	L	L	D	Y	A	T	N	V	E	Q	Y	P	A	F	Q	A	Y	F	R	E	Y	Q	P	P	L	L	A	V	W	G	E	H
Query Conservation
Alig confidence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	A	T	P	N	E	F	L	I	A	Y	Y	G	D	D	P	K	G	W	G	P	A	S	S	M	A	G	L	I	N	T	E	I	P	L	M	L	T	V	S	E	F
Template Known Secondary structure		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	.	.										S		G	G	G		G	G	G											S								T	T
Template Predicted Secondary structure		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
		263	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.							.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.					.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.
Predicted Secondary structure																		.	.	.	.	.	.																			.	.	.	.
Query Sequence		D	P	F	F	L	P	P	G	A	E	A	W	K	R	D	I	.	.	.	.	.	.	P	D	A	D	I	R	L	Y	D	T	G	H	F	A	L	E	T	H	.	.	.	.	S	E	V	I	I	P	T	I	R	Q	F	L	D	D	H	V
Query Conservation																		.	.	.	.	.	.																			.	.	.	.
Alig confidence																		.	.	.	.	.	.																			.	.	.	.
Template Conservation
Template Sequence		D	P	E	D	F	Q	R	Q	A	A	Q	F	V	C	A	W	G	M	A	H	A	A	Y	P	E	M	H	Y	L	V	G	H	N	H	L	S	P	A	Q	S	I	G	T	E	I	K	A	I	G	R	M	V	A	G	F	V	R	R	V	T
Template Known Secondary structure																						S	S								T	T			S	S					T	T	T	S	S																T
Template Predicted Secondary structure
		313
Predicted Secondary structure
Query Sequence		S
Query Conservation
Alig confidence
Template Conservation
Template Sequence		R
Template Known Secondary structure
Template Predicted Secondary structure

No model constructed - rank, confidence too low

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Powell HR et al. Journal of Molecular Biology (2025) in press DOI: https://doi.org/10.1016/j.jmb.2025.168960
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