Job Description Undefined Confidence 99.84% Date Thu Nov 13 08:26:15 GMT 2025 Rank 131 Aligned Residues 219 % Identity 20% Template c4wfiA_ PDB info PDB header: hydrolase Chain: A: PDB Molecule: Cutinase PDBTitle: crystal structure of pet-degrading cutinase cut190 s226p mutant in ca(2+)-free state PDB Entry: PDBe RCSB PDBj Resolution UNK

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		13	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.								.	.	40	.	.	.	.	.	.	.	.		.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.
Predicted Secondary structure																											.	.	.	.	.	.	.												.
Query Sequence		E	T	R	G	D	H	A	P	G	G	R	A	S	A	A	P	Q	V	H	Y	R	V	E	Q	V	.	.	.	.	.	.	.	G	D	V	K	V	F	Y	R	E	A	G	.	D	P	A	A	P	A	V	L	L	L	H	G	F	A	A	S
Query Conservation																											.	.	.	.	.	.	.												.
Alig confidence																											.	.	.	.	.	.	.												.
Template Conservation
Template Sequence		E	R	G	P	D	P	T	E	D	S	I	E	A	I	R	G	P	F	S	V	A	T	E	R	V	S	S	F	A	S	G	F	G	G	G	T	I	Y	Y	P	R	E	T	D	E	G	T	F	G	A	V	A	V	A	P	G	F	T	A	S
Template Known Secondary structure				S	S										S	S		S	S												T	T	T												T	T											S		S	S	G
Template Predicted Secondary structure
		65	.	.	.	.	70	.	.	.	.	.	.	.		.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.		.	.	.	.	.	.	120	.	.
Predicted Secondary structure															.																																					.
Query Sequence		S	Y	M	W	R	D	V	I	E	A	L	A	D	.	G	Y	H	V	I	A	P	D	L	P	A	F	G	Y	T	E	A	P	A	R	G	Q	Y	D	Y	T	F	A	N	L	T	K	T	I	E	R	.	F	T	D	Q	L	K	L	T	R
Query Conservation															.																																					.
Alig confidence															.																																					.
Template Conservation
Template Sequence		Q	G	S	M	S	W	Y	G	E	R	V	A	S	Q	G	F	I	V	F	T	I	D	T	N	T	R	L	D	Q	P	G	Q	R	G	R	Q	L	L	A	A	L	D	Y	L	V	E	R	S	D	R	K	V	R	E	R	L	D	P	N	R
Template Known Secondary structure		G	G			T	T							T	T	T								S		S																						T							T	T
Template Predicted Secondary structure
		123	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.
Predicted Secondary structure
Query Sequence		Y	A	M	A	V	H	D	Y	G	A	P	V	G	W	R	L	A	L	A	H	P	S	R	I	T	A	I	I	S	Q	N	G	N	A	Y	E	E	G	L	S	A	G	W	E	P	I	R	K	Y	W	N	D	P	T	A	E	N	R	Q	A
Query Conservation
Alig confidence																								.													.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation																								.													.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		L	A	V	M	G	H	S	M	G	G	G	G	S	L	E	A	T	V	M	R	P	S	.	L	K	A	S	I	P	L	T	P	W	N	L	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Known Secondary structure								T														T	T	.		S						S				S	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Predicted Secondary structure																								.													.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
		183	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.
Predicted Secondary structure
Query Sequence		L	S	D	F	P	T	P	A	S	I	K	W	Q	Y	V	E	G	V	S	D	T	S	L	V	A	P	D	A	Y	T	L	E	G	A	Q	I	L	R	P	G	M	A	D	I	Q	L	D	L	L	L	D	Y	A	T	N	V	E	Q	Y	P
Query Conservation
Template Conservation		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Known Secondary structure		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Predicted Secondary structure		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
		243	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270		.	.	.	.	.	.	.	.	.	280					.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.
Predicted Secondary structure																														.											.	.	.	.
Query Sequence		A	F	Q	A	Y	F	R	E	Y	Q	P	P	L	L	A	V	W	G	E	H	D	P	F	F	L	P	P	G	.	A	E	A	W	K	R	D	I	P	D	.	.	.	.	A	D	I	R	L	Y	D	T	G	H	F	A	L	E	T	H	S
Query Conservation																														.											.	.	.	.
Alig confidence		.	.																											.											.	.	.	.
Template Conservation		.	.
Template Sequence		.	.	D	K	T	W	G	Q	V	Q	V	P	T	F	I	I	G	A	E	L	D	T	I	A	P	V	R	T	H	A	K	P	F	Y	E	S	L	P	S	S	L	P	K	A	Y	M	E	L	D	G	A	T	H	F	A	P	N	I	P	N
Template Known Secondary structure		.	.					T	T			S								T	T		S	S	S		T	T	T	T								S		T	T	S								T	T			T	T	G	G	G	S
Template Predicted Secondary structure		.	.
		298	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.
Predicted Secondary structure
Query Sequence		E	V	I	I	P	T	I	R	Q	F	L	D	D	H	V	S	L	H	R	Q	E	G
Query Conservation
Alig confidence
Template Conservation
Template Sequence		T	T	I	A	K	Y	V	I	S	W	L	K	R	F	V	D	E	D	T	R	Y	S
Template Known Secondary structure																	S			G	G	G
Template Predicted Secondary structure

No model constructed - rank, confidence too low

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Powell HR et al. Journal of Molecular Biology (2025) in press DOI: https://doi.org/10.1016/j.jmb.2025.168960
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