Job Description Undefined Confidence 99.93% Date Thu Nov 13 08:26:15 GMT 2025 Rank 90 Aligned Residues 274 % Identity 18% Template c4uhhA_ PDB info PDB header: hydrolase Chain: A: PDB Molecule: ESTERASE PDBTitle: structural studies of a thermophilic esterase from thermogutta terrifontis (cacodylate complex) PDB Entry: PDBe RCSB PDBj Resolution UNK

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		40	.	.	.	.	.	.	.	.	.	50	.	.			.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.				.	70	.	.	.	.	.	.	.		.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.
Predicted Secondary structure															.	.																	.	.	.										.
Query Sequence		V	K	V	F	Y	R	E	A	G	D	P	A	A	.	.	P	A	V	L	L	L	H	G	F	A	A	S	S	Y	M	W	.	.	.	R	D	V	I	E	A	L	A	D	.	G	Y	H	V	I	A	P	D	L	P	A	F	G	Y	T	E
Query Conservation															.	.																	.	.	.										.
Alig confidence															.	.																	.	.	.										.
Template Conservation
Template Sequence		E	E	L	A	F	E	D	T	G	T	T	G	G	L	L	P	V	L	L	L	V	H	G	F	P	L	D	R	T	M	M	W	K	K	A	Q	R	E	E	L	C	D	E	E	F	F	R	V	I	V	P	D	L	R	G	F	G	E	S	Q
Template Known Secondary structure			.								S	.	.	S	S	.					.				T	T			G	G	G	.	G		.							T	T	T	.	.	S						.	.	T	T	S	T	T	S	.
Template Predicted Secondary structure
		94	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120							.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.
Predicted Secondary structure																													.	.	.	.	.	.																									.	.	.
Query Sequence		A	P	A	R	G	Q	Y	D	Y	T	F	A	N	L	T	K	T	I	E	R	F	T	D	Q	L	K	L	.	.	.	.	.	.	T	R	Y	A	M	A	V	H	D	Y	G	A	P	V	G	W	R	L	A	L	A	H	P	S	.	.	.
Query Conservation																													.	.	.	.	.	.																									.	.	.
Alig confidence					.	.																							.	.	.	.	.	.																									.	.	.
Template Conservation					.	.
Template Sequence		V	I	P	.	.	G	V	A	T	M	E	A	M	M	A	D	D	L	A	G	L	C	C	N	H	H	L	G	L	L	T	T	G	K	K	I	V	L	G	G	L	S	M	G	G	Y	V	A	F	A	F	A	R	R	K	K	Y	R	R	D
Template Known Secondary structure		.			.	.	S								.									.			.		T		.	.		S		.							T												.	.			G	.	.
Template Predicted Secondary structure					.	.
				145	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.
Predicted Secondary structure		.	.																																																					.	.	.	.	.	.
Query Sequence		.	.	R	I	T	A	I	I	S	Q	N	G	N	A	Y	E	E	G	L	S	A	G	W	E	P	I	R	K	Y	W	N	D	P	T	A	E	N	R	Q	A	L	S	D	F	P	T	P	A	S	I	K	W	Q	Y	.	.	.	.	.	.
Query Conservation		.	.																																																					.	.	.	.	.	.
Alig confidence		.	.																																																					.	.	.	.	.	.
Template Conservation
Template Sequence		D	R	R	L	A	G	L	L	I	L	C	D	T	R	R	A	R	R	P	D	S	P	E	A	K	E	N	R	R	R	R	R	R	V	A	E	E	R	R	V	R	R	E	E	G	P	G	F	I	A	E	E	E	M	I	I	P	R	R	L
Template Known Secondary structure		G	G	.					.				S			.	S	S	.													.	.	.				.	.						.		T							.			.			.
Template Predicted Secondary structure
						197	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.
Predicted Secondary structure		.	.	.	.
Query Sequence		.	.	.	.	V	E	G	V	S	D	T	S	L	V	A	P	D	A	Y	T	L	E	G	A	Q	I	L	R	P	G	M	A	D	I	Q	L	D	L	L	L	D	Y	A	T	N	V	E	Q	Y	P	A	F	Q	A	Y	F	R	E	Y	Q
Query Conservation		.	.	.	.
Alig confidence		.	.	.	.																																										.	.
Template Conservation																																															.	.
Template Sequence		C	C	C	E	E	S	T	F	R	N	H	P	E	V	I	E	E	K	K	I	R	R	Q	M	M	I	L	S	S	A	P	P	E	E	G	V	A	A	A	A	L	G	M	A	E	.	.	E	R	P	D	S	T	D	L	L	P	A	L	S
Template Known Secondary structure		S		.	.													.	.				.			.			T	.	S				.											T	.	.	.	.	.	.		G	G	G	G	G	G
Template Predicted Secondary structure																																															.	.
		253	.		.	.		.	.	.	260	.	.	.	.	.	.	.	.	.	270	.				.	.	.	.	.		.	.	.	280	.		.	.			.	.	.			.	.	.	290	.	.	.		.	.	.	.	.	.
Predicted Secondary structure				.			.																.	.	.						.						.			.	.				.	.								.							.
Query Sequence		P	P	.	L	L	.	A	V	W	G	E	H	D	P	F	F	L	P	P	G	A	.	.	.	E	A	W	K	R	.	D	I	P	D	A	.	D	I	.	.	R	L	Y	.	.	D	T	G	H	F	A	L	.	E	T	H	S	E	V	.
Query Conservation				.			.																.	.	.						.						.			.	.				.	.								.							.
Alig confidence				.			.																.	.	.						.						.			.	.				.	.								.							.
Template Conservation
Template Sequence		C	P	P	T	L	V	L	L	V	G	Q	F	D	A	I	S	P	P	P	E	E	E	E	M	E	A	M	A	R	R	T	I	P	Q	S	Q	Q	F	V	V	V	I	P	P	D	D	A	G	H	L	P	P	M	M	E	Q	P	E	R	R
Template Known Secondary structure		S		.					.			T	T		S	S	S			.	.	.								T	.	T	S	T	T			.				.		T	.	.	T			S					.		S				.
Template Predicted Secondary structure
		300	.	.	.	.	.		.	.	.	.	310	.	.	.	.	.	.	.
Predicted Secondary structure								.
Query Sequence		I	I	P	T	I	R	.	Q	F	L	D	D	H	V	S	L	H	R	Q
Query Conservation								.
Alig confidence								.
Template Conservation
Template Sequence		V	T	Q	A	I	R	E	E	W	L	R	K	K	V	V	H	T	E	E
Template Known Secondary structure									.					.	.					.
Template Predicted Secondary structure

No model constructed - rank, confidence too low

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Powell HR et al. Journal of Molecular Biology (2025) in press DOI: https://doi.org/10.1016/j.jmb.2025.168960
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