Job Description Undefined Confidence 99.82% Date Thu Nov 13 08:26:15 GMT 2025 Rank 354 Aligned Residues 200 % Identity 16% Template c4p92A_ PDB info PDB header: hydrolase Chain: A: PDB Molecule: Carboxymethylenebutenolidase PDBTitle: crystal structure of dienelactone hydrolase c123s mutant at 1.65 a resolution PDB Entry: PDBe RCSB PDBj Resolution UNK

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		28	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50			.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.		.	.	80	.	.	.	.
Predicted Secondary structure																									.	.																												.
Query Sequence		P	Q	V	H	Y	R	V	E	Q	V	G	D	V	K	V	F	Y	R	E	A	G	D	P	.	.	A	A	P	A	V	L	L	L	H	G	F	A	A	S	S	Y	M	W	R	D	V	I	E	A	L	A	D	.	G	Y	H	V	I	A	P
Query Conservation																									.	.																												.
Alig confidence																									.	.																												.
Template Conservation
Template Sequence		L	T	E	G	I	S	I	Q	S	Y	D	G	H	T	F	G	A	L	V	G	S	P	A	K	A	P	A	P	V	I	V	I	A	Q	E	I	F	G	V	N	A	F	M	R	E	T	V	S	W	L	V	D	Q	G	Y	A	A	V	C	P
Template Predicted Secondary structure
		85	.	.	.	.	90	.	.	.	.	.	.																	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120		.	.	.	.	.	.	.
Predicted Secondary structure														.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.																									.
Query Sequence		D	L	P	A	F	G	Y	T	E	A	P	A	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	R	G	Q	Y	D	Y	T	F	A	N	L	T	K	T	I	E	R	F	T	D	Q	L	K	L	.	T	R	Y	A	M	A	V
Query Conservation														.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.																									.
Alig confidence														.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.																									.
Template Conservation
Template Sequence		D	L	Y	A	R	Q	A	P	G	T	A	L	D	P	Q	D	E	R	Q	R	E	Q	A	Y	K	L	W	Q	A	F	D	M	E	A	G	V	G	D	L	E	A	A	I	R	Y	A	R	H	Q	P	Y	S	N	G	K	V	G	L	V	G
Template Predicted Secondary structure
		128	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.
Predicted Secondary structure
Query Sequence		H	D	Y	G	A	P	V	G	W	R	L	A	L	A	H	P	S	R	I	T	A	I	I	S	Q	N	G	N	A	Y	E	E	G	L	S	A	G	W	E	P	I	R	K	Y	W	N	D	P	T	A	E	N	R	Q	A	L	S	D	F	P
Query Conservation
Alig confidence																.	.																.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation																.	.																.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		Y	S	L	G	G	A	L	A	F	L	V	A	A	K	.	.	G	Y	V	D	R	A	V	G	Y	Y	G	V	G	L	E	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Predicted Secondary structure																.	.																.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
		188	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.
Predicted Secondary structure
Query Sequence		T	P	A	S	I	K	W	Q	Y	V	E	G	V	S	D	T	S	L	V	A	P	D	A	Y	T	L	E	G	A	Q	I	L	R	P	G	M	A	D	I	Q	L	D	L	L	L	D	Y	A	T	N	V	E	Q	Y	P	A	F	Q	A	Y
Query Conservation
Alig confidence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	K	Q	L	N	K
Template Predicted Secondary structure		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
		248	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280				.	.	.		.	.	.	.	.	.	290	.	.	.	.	.	.
Predicted Secondary structure																																			.	.	.				.														.	.	.	.	.	.	.
Query Sequence		F	R	E	Y	Q	P	P	L	L	A	V	W	G	E	H	D	P	F	F	L	P	P	G	A	E	A	W	K	R	D	I	P	D	.	.	.	A	D	I	.	R	L	Y	D	T	G	H	F	A	L	E	T	H	.	.	.	.	.	.	.
Query Conservation																																			.	.	.				.														.	.	.	.	.	.	.
Alig confidence																																			.	.	.				.														.	.	.	.	.	.	.
Template Conservation
Template Sequence		V	P	E	V	K	H	P	A	L	F	H	M	G	G	Q	D	H	F	V	P	A	P	S	R	Q	L	I	T	E	G	F	G	A	N	P	L	L	Q	V	H	W	Y	E	E	A	G	H	S	F	A	R	T	S	S	S	G	Y	V	A	S
Template Predicted Secondary structure
			297	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.
Predicted Secondary structure		.
Query Sequence		.	S	E	V	I	I	P	T	I	R	Q	F	L	D	D	H	V	S
Query Conservation		.
Alig confidence		.
Template Conservation
Template Sequence		A	A	A	L	A	N	E	R	T	L	D	F	L	A	P	L	Q	S
Template Predicted Secondary structure

No model constructed - rank, confidence too low

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Powell HR et al. Journal of Molecular Biology (2025) in press DOI: https://doi.org/10.1016/j.jmb.2025.168960
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