Job Description Undefined Confidence 99.80% Date Thu Nov 13 08:26:15 GMT 2025 Rank 494 Aligned Residues 174 % Identity 12% Template c3d2bA_ PDB info PDB header: hydrolase Chain: A: PDB Molecule: Lipase PDBTitle: structure of 2d9, a thermostable mutant of bacillus subtilis lipase obtained through directed evolution PDB Entry: PDBe RCSB PDBj Resolution UNK

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		52	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.		.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110
Predicted Secondary structure																												.
Query Sequence		A	P	A	V	L	L	L	H	G	F	A	A	S	S	Y	M	W	R	D	V	I	E	A	L	A	D	.	G	Y	H	V	I	A	P	D	L	P	A	F	G	Y	T	E	A	P	A	R	G	Q	Y	D	Y	T	F	A	N	L	T	K	T
Query Conservation																												.
Alig confidence																												.								.	.											.	.
Template Conservation																																				.	.											.	.
Template Sequence		H	N	P	V	V	M	V	H	G	I	G	G	A	S	S	N	F	A	G	I	K	S	Y	L	V	S	Q	G	W	S	R	D	K	L	.	.	Y	A	V	D	F	W	D	K	T	G	.	.	T	N	Y	N	N	G	P	V	L	S	R	F
Template Known Secondary structure											T	T			G	G	G	G										T	T			G	G	G		.	.								T	T		.	.
Template Predicted Secondary structure																																				.	.											.	.
		111	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.			.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.
Predicted Secondary structure																																		.	.
Query Sequence		I	E	R	F	T	D	Q	L	K	L	T	R	Y	A	M	A	V	H	D	Y	G	A	P	V	G	W	R	L	A	L	A	H	.	.	P	S	R	I	T	A	I	I	S	Q	N	G	N	A	Y	E	E	G	L	S	A	G	W	E	P	I
Query Conservation																																		.	.
Alig confidence																																		.	.																									.	.
Template Conservation																																																												.	.
Template Sequence		V	Q	K	V	L	D	E	T	G	A	K	K	V	D	I	V	A	H	S	M	G	G	A	N	T	L	Y	Y	I	K	Y	L	D	G	G	N	K	V	A	N	V	V	T	L	G	G	A	N	R	L	T	T	G	K	A	P	P	G	.	.
Template Known Secondary structure												S								T													S	S	G	G	G	T								S			G	G	G	T		S	B					.	.
Template Predicted Secondary structure																																																												.	.
		169	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.
Predicted Secondary structure
Query Sequence		R	K	Y	W	N	D	P	T	A	E	N	R	Q	A	L	S	D	F	P	T	P	A	S	I	K	W	Q	Y	V	E	G	V	S	D	T	S	L	V	A	P	D	A	Y	T	L	E	G	A	Q	I	L	R	P	G	M	A	D	I	Q	L
Query Conservation
Template Conservation		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Known Secondary structure		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Predicted Secondary structure		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
		229	230	.	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.		.	.	.	.
Predicted Secondary structure																																																									.
Query Sequence		D	L	L	L	D	Y	A	T	N	V	E	Q	Y	P	A	F	Q	A	Y	F	R	E	Y	Q	P	P	L	L	A	V	W	G	E	H	D	P	F	F	L	P	P	G	A	E	A	W	K	R	D	I	P	D	A	D	I	.	R	L	Y	D
Query Conservation																																																									.
Alig confidence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.						.																				.	.	.	.	.									.
Template Conservation		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.						.																				.	.	.	.	.
Template Sequence		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	T	D	P	N	Q	.	K	I	L	Y	T	S	I	Y	S	S	D	D	M	I	V	M	N	Y	L	.	.	.	.	.	S	R	L	D	G	A	R	N	V	Q	I	H	G
Template Known Secondary structure		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S		T	T		.										T	T		S	S	S					.	.	.	.	.				B	T	S							S
Template Predicted Secondary structure		.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.						.																				.	.	.	.	.
		288	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.
Predicted Secondary structure
Query Sequence		T	G	H	F	A	L	E	T	H	S	E	V	I	I	P	T	I	R	Q	F	L	D	D	H	V	S	L	H
Query Conservation
Alig confidence												.
Template Conservation												.
Template Sequence		V	G	H	M	G	L	L	Y	S	S	.	Q	V	Y	S	L	I	K	E	G	L	N	G	G	G	Q	N	T
Template Known Secondary structure				T	G	G	G	G	G			.											T	T	T		B
Template Predicted Secondary structure												.

No model constructed - rank, confidence too low

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Please cite: Phyre2.2: A Community Resource for Template-based Protein Structure Prediction
Powell HR et al. Journal of Molecular Biology (2025) in press DOI: https://doi.org/10.1016/j.jmb.2025.168960
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