Job Description Q21408 Confidence 99.87% Date Sat Apr 13 12:50:02 BST 2013 Rank 2 Aligned Residues 443 % Identity 25% Template c1hciB_ PDB info PDB header:triple-helix coiled coil Chain: B: PDB Molecule:alpha-actinin 2; PDBTitle: crystal structure of the rod domain of alpha-actinin Resolution 2.8 Å Model Dimensions (Å) X:221.086 Y:103.814 Z:78.625

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		1832	.	.	.	.	.	.	.	1840	.	.	.	.	.	.	.	.	.	1850	.	.	.	.	.	.	.	.	.	1860	.	.	.	.	.	.	.	.	.	1870	.	.	.	.	.	.	.	.	.	1880	.	.	.	.	.	.	.	.	.	1890	.	.	.	.	.	.	.	.	.	1900	.	.	.	.
Predicted Secondary structure																																																																											.	.	.	.	.	.	.
Query SS confidence																																																																											.	.	.	.	.	.	.
Query Sequence		D	E	S	E	A	F	Q	A	F	L	G	D	V	E	E	E	E	A	W	M	N	E	K	Q	Q	I	L	G	S	D	N	F	G	E	N	M	A	G	V	Q	G	L	L	K	K	H	D	T	F	Q	V	D	L	E	L	H	K	Q	R	V	A	D	L	I	N	K	G	D	T	L	I	H	N	.	.	.	.	.	.	.
Query Conservation																																																																											.	.	.	.	.	.	.
Alig confidence																																																																											.	.	.	.	.	.	.
Template Conservation
Template Sequence		E	N	E	R	L	M	E	E	Y	E	R	L	A	S	E	L	L	E	W	I	R	R	T	I	P	W	L	E	N	R	T	P	E	K	T	M	Q	A	M	Q	K	K	L	E	D	F	R	D	Y	R	R	K	H	K	P	P	K	V	Q	E	K	C	Q	L	E	I	N	F	N	T	L	Q	T	K	L	R	I	S	N	R
Template Known Secondary structure																			T									T	T						S	S	S	T	T	T	T													T											T	T	T					T	T	T	T	T	T	T	T	T	S
Template Predicted Secondary structure
Template SS confidence
		278	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.
								1905	.	.	.	.	1910	.	.	.	.	.	.	.	.	.	1920	.	.	.	.	.	.	.	.	.	1930	.	.	.	.	.	.	.	.	.	1940	.		.	.	.	.	.	.	.	.	1950	.	.	.	.	.	.	.	.	.	1960	.	.	.	.	.	.	.		.	.	1970	.	.	.	.	.	.
Predicted Secondary structure		.	.	.	.	.	.																																						.																											.
Query SS confidence		.	.	.	.	.	.																																						.																											.
Query Sequence		.	.	.	.	.	.	G	N	H	H	A	P	H	I	K	Q	R	C	D	Q	L	R	A	R	L	I	E	I	E	S	M	A	E	G	R	L	A	K	L	R	D	N	S	.	A	Y	L	Q	F	M	W	K	C	D	V	V	E	S	W	I	A	E	K	E	Q	Q	V	R	S	E	.	D	F	G	R	D	L	S	S	V
Query Conservation		.	.	.	.	.	.																																						.																											.
Alig confidence		.	.	.	.	.	.																																						.																											.
Template Conservation
Template Sequence		P	A	F	M	P	S	E	G	K	M	V	S	D	I	A	G	A	W	Q	R	L	E	Q	A	E	K	G	Y	E	E	W	L	L	N	E	I	R	R	L	E	R	L	E	H	L	A	E	K	F	R	Q	K	A	S	T	H	E	T	W	A	Y	G	K	E	Q	I	L	L	Q	K	D	Y	E	S	A	S	L	T	E	V
Template Known Secondary structure								G	G	G		S	S	S													T	T																																		T	T									G	G	G	S
Template Predicted Secondary structure
Template SS confidence
		358	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.
		1977	.	.	1980	.	.	.	.	.	.	.	.	.	1990	.	.	.	.	.	.	.	.	.	2000	.	.	.	.	.	.	.	.	.	2010	.	.	.	.	.	.	.	.	.	2020	.	.	.	.	.	.	.	.	.	2030	.	.	.	.	.	.	.	.	.	2040	.	.	.	.	.	.	.	.	.	2050	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		Q	I	L	L	T	K	Q	E	A	F	D	A	G	L	N	A	F	E	H	E	G	I	Q	R	I	T	E	L	K	D	Q	L	V	S	S	E	H	Q	Q	S	P	A	I	E	K	R	H	T	N	V	I	Q	R	W	Q	N	L	L	A	H	S	E	A	R	R	Q	K	L	L	K	M	Q	Q	Q	F	K	Q	I	E	E
Query Conservation
Alig confidence																		.
Template Conservation																		.
Template Sequence		R	A	L	L	R	K	H	E	A	F	E	S	D	L	A	A	.	H	Q	D	R	V	E	Q	I	A	A	I	A	Q	E	L	N	E	L	D	Y	H	D	A	V	N	V	N	D	R	C	Q	K	I	C	D	Q	W	D	R	L	G	T	L	T	Q	K	R	R	E	A	L	E	R	M	E	K	L	L	E	T	I	D	Q
Template Known Secondary structure																		.																		T	T		T	T
Template Predicted Secondary structure																		.
Template SS confidence
		438	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.		.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480	.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.
		2057	.	.	2060	.	.	.	.	.	.	.	.	.	2070	.	.	.	.	.	.	.	.	.	2080	.	.	.	.	.	.	.	.	.	2090	.	.	.	.	.	.	.	.	.	2100	.	.	.	.	.	.	.	.	.	2110	.	.	.	.	.	.	.	.	.	2120	.	.	.								.	.	.	.	.	.
Predicted Secondary structure																																																																					.	.	.	.	.	.	.
Query SS confidence																																																																					.	.	.	.	.	.	.
Query Sequence		L	Y	L	A	F	A	K	K	A	S	T	F	N	S	W	F	E	N	A	E	E	D	L	T	D	P	V	R	C	N	S	L	E	E	I	R	A	L	R	D	A	H	G	E	F	Q	R	S	L	S	S	A	E	E	D	F	R	Q	L	Q	D	L	D	R	R	I	K	.	.	.	.	.	.	.	S	F	N	V	G	P
Query Conservation																																																																					.	.	.	.	.	.	.
Alig confidence																																																																					.	.	.	.	.	.	.
Template Conservation
Template Sequence		L	H	L	E	F	A	K	R	A	A	P	F	N	N	W	M	E	G	A	M	E	D	L	Q	D	M	F	I	V	H	S	I	E	E	I	Q	S	L	I	T	A	H	E	Q	F	K	A	T	L	P	E	A	D	G	E	R	Q	S	I	M	A	I	Q	N	E	V	E	K	V	I	Q	S	Y	N	I	R	I	S	S	S
Template Known Secondary structure																								T	T						S	S																																									T	T	T			S		S
Template Predicted Secondary structure
Template SS confidence
		517	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540	.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.
		2130	.	.	.	.	.	.	.	.	.	2140	.	.	.	.	.	.	.	.	.	2150	.	.	.	.	.	.	.	.	.	2160	.	.	.	.	.	.	.	.	.	2170	.	.	.	.	.	.	.	.	.	2180	.	.	.	.	.	.	.	.	.	2190	.	.	.	.	.					.	.	.	.	2200	.	.	.	.	.
Predicted Secondary structure																																																																				.	.	.	.
Query SS confidence																																																																				.	.	.	.
Query Sequence		N	P	Y	T	W	F	T	M	D	A	L	E	D	T	W	R	N	L	Q	R	I	I	K	E	R	E	Q	E	L	A	R	E	H	Q	R	Q	E	E	N	D	K	L	R	R	E	F	A	K	L	A	N	A	F	H	A	W	L	T	N	T	R	Q	E	M	M	E	.	.	.	.	A	G	G	T	L	E	E	Q	L	D
Query Conservation																																																																				.	.	.	.
Alig confidence																																																																				.	.	.	.
Template Conservation
Template Sequence		N	P	Y	S	T	V	T	M	D	E	L	R	T	K	W	D	K	V	K	Q	L	V	P	I	R	D	Q	S	L	Q	E	E	L	A	R	Q	H	A	N	E	R	L	R	R	Q	F	A	A	Q	A	N	A	I	G	P	W	I	Q	N	K	M	E	E	I	A	R	S	S	I	Q	I	T	G	A	L	E	D	Q	M	N
Template Known Secondary structure			T	T		S	S																																																												S		S		T	T	T	T	S	T
Template Predicted Secondary structure
Template SS confidence
		597	.	.	600	.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.	.	.	.	.	.	.	.	630	.	.	.	.	.	.	.	.	.	640	.	.	.	.	.	.	.	.	.	650	.	.	.	.	.	.	.	.	.	660	.	.	.	.	.	.	.	.	.	670	.	.	.	.	.	.
		2206	.	.	.	2210	.	.	.	.	.	.	.	.	.	2220	.	.	.	.	.	.	.	.	.	2230	.	.	.	.	.	.	.	.	.	2240	.	.	.	.	.	.	.	.	.	2250	.	.	.	.	.	.	.	.	.	2260	.	.	.	.	.	.	.	.	.	2270	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		A	V	E	R	K	A	K	E	I	K	A	N	K	G	Q	L	R	Q	I	E	E	K	G	A	M	L	E	R	N	L	I	L	D	N	R	Y	T	E	H	S	T	V	G	I	A	Q	A	W	D	Q	L	D	Q	L	A	M	R	M	Q	H	N	L	E	Q	Q	I	Q	A	R	N
Query Conservation
Alig confidence
Template Conservation
Template Sequence		Q	L	K	Q	Y	E	H	N	I	I	N	Y	K	N	N	I	D	K	L	E	G	D	H	Q	L	I	Q	E	A	L	V	F	D	N	K	H	T	N	Y	T	M	E	H	I	R	V	G	W	E	L	L	L	T	T	I	A	R	T	I	N	E	V	E	T	Q	I	L	T	R	D
Template Known Secondary structure																														T	T					T	T		S																												T	T	S
Template Predicted Secondary structure
Template SS confidence
		677	.	.	680	.	.	.	.	.	.	.	.	.	690	.	.	.	.	.	.	.	.	.	700	.	.	.	.	.	.	.	.	.	710	.	.	.	.	.	.	.	.	.	720	.	.	.	.	.	.	.	.	.	730	.	.	.	.	.	.	.	.	.	740	.	.	.	.	.	.