Job Description O07325 Confidence 99.10% Date Tue Jul 17 17:05:02 BST 2012 Rank 50 Aligned Residues 344 % Identity 16% Template c4e1jA_ PDB info PDB header:transferase Chain: A: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of glycerol kinase in complex with glycerol from2 sinorhizobium meliloti 1021 Resolution 2.33 Å

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		4	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.		.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.		.	.	.	.	80	.
Predicted Secondary structure																																													.																														.
Query SS confidence																																													.																														.
Query Sequence		H	Y	Y	V	S	I	D	I	G	S	S	S	V	K	T	I	V	G	E	K	F	H	N	G	I	N	V	I	G	T	G	Q	T	Y	T	S	G	I	K	N	G	L	I	.	D	D	F	D	I	A	R	Q	A	I	K	D	T	I	K	K	A	S	I	A	S	G	V	D	I	K	E	V	F	.	L	K	L	P	I	I
Query Conservation																																													.																														.
Alig confidence																					.																								.																														.
Template Conservation																					.
Template Sequence		G	Y	I	L	A	I	D	Q	G	T	T	S	T	R	A	I	V	F	D	.	G	N	Q	K	I	A	G	V	G	Q	K	E	F	K	Q	H	F	P	K	S	G	W	V	E	H	D	P	E	E	I	W	Q	T	V	V	S	T	V	K	E	A	I	E	K	S	G	I	T	A	N	D	I	A	A	I	G	I	T	N	Q
Template Known Secondary structure											S	S									.	T	T	S													B		S	S	T	T																							T	T	T			G	G	G
Template Predicted Secondary structure																					.
Template SS confidence
		26	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.		.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.
		82	.	.	.	.	.	.	.	90	.		.	.	.	.	.	.	.	.	100						.	.	.	.	.	.	.	.	.	110	.	.																									.	.	.	.	.	.	.	120	.	.								.	.
Predicted Secondary structure												.										.	.	.	.	.													.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.											.	.	.	.	.	.	.
Query SS confidence												.										.	.	.	.	.													.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.											.	.	.	.	.	.	.
Query Sequence		G	T	E	V	Y	D	E	S	N	E	.	I	D	F	Y	E	D	T	E	I	.	.	.	.	.	N	G	S	H	I	E	K	V	L	E	G	I	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	R	E	K	N	D	V	Q	E	T	E	.	.	.	.	.	.	.	V	I
Query Conservation												.										.	.	.	.	.													.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.											.	.	.	.	.	.	.
Alig confidence												.										.	.	.	.	.													.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.											.	.	.	.	.	.	.
Template Conservation
Template Sequence		R	E	T	V	V	V	W	D	R	E	T	G	K	P	I	H	N	A	I	V	W	Q	D	R	R	T	A	A	F	C	D	K	L	K	K	K	G	L	E	K	T	F	V	K	K	T	G	L	L	L	D	P	Y	F	S	G	T	K	L	N	W	L	L	S	N	V	K	G	A	Q	V	R	A	A	K	G	E	L	C	F
Template Known Secondary structure		S	S							T	T	T			B	S	S					T	T			T	T										T	T										S	S		S	S	T	T	S												S	T	T							T	T
Template Predicted Secondary structure
Template SS confidence
		105	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.
		125	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.																																.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.
Predicted Secondary structure																																	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Query SS confidence																																	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Query Sequence		N	V	F	P	I	R	F	I	V	D	K	E	N	E	V	S	D	P	K	E	L	I	A	R	H	S	L	K	V	E	A	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	G	V	I	A	I	Q	K	S	I	L	I	N	M	I	K	C	V	E
Query Conservation																																	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Alig confidence										.	.	.	.	.	.	.	.	.	.														.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation										.	.	.	.	.	.	.	.	.	.
Template Sequence		G	T	I	D	T	F	L	I	.	.	.	.	.	.	.	.	.	.	W	R	L	T	G	G	E	C	F	C	T	D	A	T	N	A	S	R	T	L	L	Y	N	I	A	E	N	A	W	D	D	E	L	T	E	V	L	R	V	P	K	E	X	L	P	E	V	K	D	C	A	A	D	F	G	V	T	D	P	S	L	F
Template Known Secondary structure										.	.	.	.	.	.	.	.	.	.				T	T	T											T	T	S	S	S			T	T	T	T											T			G	G	G	S						S	B	S				B
Template Predicted Secondary structure										.	.	.	.	.	.	.	.	.	.
Template SS confidence
		185	.	.	.	.	190	.	.											.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.
		174	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220																					.	.	.	.	.	.	.	.	.	230	.	.	.
Predicted Secondary structure																																																	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Query SS confidence																																																	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Query Sequence		A	C	G	V	D	V	L	D	V	Y	S	D	A	Y	N	Y	G	S	I	L	T	A	T	E	K	E	L	G	A	C	V	I	D	I	G	E	D	V	T	Q	V	A	F	Y	E	R	G	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	E	L	V	D	A	D	S	I	E	M	A	G	R
Query Conservation																																																	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Alig confidence																													.	.																			.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation																													.	.
Template Sequence		G	A	A	I	P	I	L	G	V	A	G	D	Q	Q	A	A	T	I	G	Q	A	C	F	K	P	G	X	.	.	L	K	S	T	Y	G	T	G	C	F	A	L	L	N	T	G	K	D	X	V	R	S	K	N	R	L	L	T	T	I	A	Y	R	L	D	G	E	T	T	Y	A	L	E	G	S	I	F	V	A	G	A
Template Known Secondary structure		T	S			B															T	T			S	T	T		.	.						S	S	S								T	T						S	S	S										T	T											S		S
Template Predicted Secondary structure																													.	.
Template SS confidence
		255	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.			.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.
		234	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.			.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.
Predicted Secondary structure																																										.	.
Query SS confidence																																										.	.
Query Sequence		D	I	T	D	D	I	A	Q	G	L	N	T	S	Y	E	T	A	E	K	V	K	H	Q	Y	G	H	A	F	Y	D	S	A	S	D	Q	D	I	F	T	V	.	.	E	Q	V	D	S	D	E	T	V	Q	Y	T	Q	K	D	L	S	D	F	I	E	A	R	V	E	E	I	F	F	E	V	F	D	V	L	Q	D	L
Query Conservation																																										.	.
Alig confidence				.																																						.	.
Template Conservation				.
Template Sequence		A	V	.	Q	W	L	R	D	G	L	K	V	I	T	G	S	L	A	E	S	A	D	P	S	Q	E	V	Y	L	V	P	A	F	T	G	L	G	A	P	H	W	D	P	D	A	R	G	A	I	F	G	X	T	R	N	T	G	P	A	E	F	A	R	A	A	L	E	A	V	C	Y	Q	T	R	D	L	L	E	A	X
Template Known Secondary structure				.						T	T										T	S		T	T									T	T		B		T	T	T		B	T	T										T	T
Template Predicted Secondary structure				.
Template SS confidence
		333	.		.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.
		312	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370
Predicted Secondary structure
Query SS confidence
Query Sequence		G	L	T	K	V	N	G	G	F	I	V	T	G	G	S	A	N	L	L	G	V	K	E	L	L	S	D	M	V	S	E	K	V	R	I	H	T	P	S	Q	M	G	I	R	K	P	E	F	S	S	A	I	S	T	I	S	S	S	I
Query Conservation
Alig confidence			.																																							.	.	.	.	.	.	.	.
Template Conservation			.																																							.	.	.	.	.	.	.	.
Template Sequence		H	.	K	D	W	R	T	V	L	R	V	D	G	G	X	V	A	S	D	W	T	X	Q	R	L	S	D	L	L	D	A	P	V	D	R	P	V	I	L	E	.	.	.	.	.	.	.	.	T	T	A	L	G	V	A	W	L	A	G
Template Known Secondary structure			.											S	G	G	G	G													T	S						S				.	.	.	.	.	.	.	.
Template Predicted Secondary structure			.																																							.	.	.	.	.	.	.	.
Template SS confidence
		417		.	.	420	.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.									.	.	.	.	460	.	.	.	.	.	.