Job Description O07325 Confidence 99.07% Date Tue Jul 17 17:05:02 BST 2012 Rank 52 Aligned Residues 354 % Identity 14% Template c3ezwD_ PDB info PDB header:transferase Chain: D: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of a hyperactive escherichia coli glycerol kinase2 mutant gly230 --> asp obtained using microfluidic crystallization3 devices Resolution 2.00 Å

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		2	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.		.	.	.	.	.	.	80
Predicted Secondary structure																																																																										.
Query SS confidence																																																																										.
Query Sequence		E	E	H	Y	Y	V	S	I	D	I	G	S	S	S	V	K	T	I	V	G	E	K	F	H	N	G	I	N	V	I	G	T	G	Q	T	Y	T	S	G	I	K	N	G	L	I	D	D	F	D	I	A	R	Q	A	I	K	D	T	I	K	K	A	S	I	A	S	G	V	D	I	K	E	.	V	F	L	K	L	P	I
Query Conservation																																																																										.
Alig confidence																																																																										.
Template Conservation
Template Sequence		E	K	K	Y	I	V	A	L	D	Q	G	T	T	S	S	R	A	V	V	M	D	H	D	A	N	I	I	S	V	S	Q	R	E	F	E	Q	I	Y	P	K	P	G	W	V	E	H	D	P	M	E	I	W	A	T	Q	S	S	T	L	V	E	V	L	A	K	A	D	I	S	S	D	Q	I	A	A	I	G	I	T	N
Template Known Secondary structure													S	S									T	T														B			S	T	T																								T	T			G	G	G
Template Predicted Secondary structure
Template SS confidence
		2	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.
		81	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.					.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.
Predicted Secondary structure																																												.	.	.	.
Query SS confidence																																												.	.	.	.
Query Sequence		I	G	T	E	V	Y	D	E	S	N	E	I	D	F	Y	E	D	T	E	I	N	G	S	H	I	E	K	V	L	E	G	I	R	E	K	N	D	V	Q	E	T	E	.	.	.	.	V	I	N	V	F	P	I	R	F	I	V	D	K	E	N	E	V	S	D	P	K	E	L	I	A	R	H	S	L	K	V	E	A	G
Query Conservation																																												.	.	.	.
Alig confidence																																												.	.	.	.
Template Conservation
Template Sequence		Q	R	E	T	T	I	V	W	E	K	E	T	G	K	P	I	Y	N	A	I	V	W	Q	C	R	R	T	A	E	I	C	E	H	L	K	R	D	G	L	E	D	Y	I	R	S	N	T	G	L	V	I	D	P	Y	F	S	G	T	K	V	K	W	I	L	D	H	V	E	G	S	R	E	R	A	R	R	G	E	L	L
Template Known Secondary structure			S	S		B					T	T	T			B	S	S					T	T			T	T										T	T										S			S	S	T	T	S												S	T	T							T	T	S
Template Predicted Secondary structure
Template SS confidence
		82	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.
		157	.	.	160	.	.	.	.	.	.	.	.										.	170	.	.	.	.	.	.	.																																								.	.	180	.	.	.	.	.	.	.	.
Predicted Secondary structure														.	.	.	.	.	.	.	.	.										.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Query SS confidence														.	.	.	.	.	.	.	.	.										.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Query Sequence		V	I	A	I	Q	K	S	I	L	I	N	M	.	.	.	.	.	.	.	.	.	I	K	C	V	E	A	C	G	V	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	D	V	L	D	V	Y	S	D	A	Y	N
Query Conservation														.	.	.	.	.	.	.	.	.										.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Alig confidence														.	.	.	.	.	.	.	.	.										.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation
Template Sequence		F	G	T	V	D	T	W	L	I	W	K	M	T	Q	G	R	V	H	V	T	D	Y	T	N	A	S	R	T	M	L	F	N	I	H	T	L	D	W	D	D	K	M	L	E	V	L	D	I	P	R	E	M	L	P	E	V	R	R	S	S	E	V	Y	G	Q	T	N	R	I	P	I	S	G	I	A	G	D	Q	Q	A
Template Known Secondary structure														T	T	T	S										G	G	G	S	S			T	T	T	T										T	T			G	G	G							S
Template Predicted Secondary structure
Template SS confidence
		162	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.
		189	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.																					.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.	.
Predicted Secondary structure																																.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Query SS confidence																																.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Query Sequence		Y	G	S	I	L	T	A	T	E	K	E	L	G	A	C	V	I	D	I	G	E	D	V	T	Q	V	A	F	Y	E	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	R	G	E	L	V	D	A	D	S	I	E	M	A	G	R	D	I	T	D	D	I	A	Q	G	L	N	T	S	Y	E
Query Conservation																																.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Alig confidence														.	.																	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation														.	.
Template Sequence		A	L	F	G	Q	L	C	V	K	E	G	M	.	.	A	K	N	T	Y	G	T	G	C	F	M	L	M	N	T	G	E	K	A	V	K	S	E	N	G	L	L	T	T	I	A	C	G	P	T	G	E	V	N	Y	A	L	E	G	A	V	F	M	A	G	A	S	I	Q	W	L	R	D	E	M	K	L	I	D	S	E
Template Known Secondary structure						T	T			S	T	T		.	.						S	S	S								T	T	S					S	S	S									T	T	S											S		S										T	S
Template Predicted Secondary structure														.	.
Template SS confidence
		249	250	.	.	.	.	.	.	.	.	.	260			.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.
		249	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.						280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310		.	.	.	.	.	.	.	.	.	320	.	.
Predicted Secondary structure																																	.	.	.	.	.																																.
Query SS confidence																																	.	.	.	.	.																																.
Query Sequence		T	A	E	K	V	K	H	Q	Y	G	H	A	F	Y	D	S	A	S	D	Q	D	I	F	T	V	E	Q	V	D	S	D	.	.	.	.	.	E	T	V	Q	Y	T	Q	K	D	L	S	D	F	I	E	A	R	V	E	E	I	F	F	E	V	F	D	V	L	Q	D	.	L	G	L	T	K	V	N	G	G	F	I	V
Query Conservation																																	.	.	.	.	.																																.
Alig confidence																																	.	.	.	.	.																																.					.	.
Template Conservation																																																																										.	.
Template Sequence		Y	F	A	T	K	V	Q	N	T	N	G	V	Y	V	V	P	A	F	T	G	L	G	A	P	Y	W	D	P	Y	A	R	G	A	I	F	G	L	T	R	G	V	N	A	N	H	I	I	R	A	T	L	E	S	I	A	Y	Q	T	R	D	V	L	E	A	M	Q	A	D	S	G	I	R	.	.	L	H	A	L	R	V
Template Known Secondary structure						T	S	S	S		T	T							T	T		B		T	T	T		B	T	T										T	T																																	.	.		S
Template Predicted Secondary structure																																																																										.	.
Template SS confidence
		331	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.			.	.	.	.	.	.
		323	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370
Predicted Secondary structure
Query SS confidence
Query Sequence		T	G	G	S	A	N	L	L	G	V	K	E	L	L	S	D	M	V	S	E	K	V	R	I	H	T	P	S	Q	M	G	I	R	K	P	E	F	S	S	A	I	S	T	I	S	S	S	I
Query Conservation
Alig confidence																										.	.			.	.	.	.	.	.	.	.	.
Template Conservation																										.	.			.	.	.	.	.	.	.	.	.
Template Sequence		D	G	G	A	V	A	N	N	F	L	M	Q	F	Q	S	D	I	L	G	T	R	V	E	R	.	.	P	E	.	.	.	.	.	.	.	.	.	V	T	A	L	G	A	A	Y	L	A	G
Template Known Secondary structure			S	G	G	G	G													T	S					.	.			.	.	.	.	.	.	.	.	.
Template Predicted Secondary structure																										.	.			.	.	.	.	.	.	.	.	.
Template SS confidence
		409	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.			.	.										.	.	.	.	.	440	.	.	.	.	.